Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10j20f
(593 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 55 4e-08
At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta... 53 1e-07
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 46 2e-05
At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g... 44 8e-05
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 43 2e-04
At4g09020.1 68417.m01489 isoamylase, putative / starch debranchi... 42 3e-04
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 41 7e-04
At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi... 40 0.002
At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi... 40 0.002
At1g76130.1 68414.m08841 alpha-amylase, putative / 1,4-alpha-D-g... 38 0.007
At1g07510.1 68414.m00804 FtsH protease, putative similar to AAA-... 32 0.25
At5g61760.1 68418.m07749 inositol polyphosphate 6-/3-/5-kinase 2... 29 2.3
At2g23630.1 68415.m02819 multi-copper oxidase type I family prot... 29 2.3
At5g09390.1 68418.m01088 CD2-binding protein-related similar to ... 28 5.4
At4g37160.1 68417.m05261 multi-copper oxidase type I family prot... 27 7.1
At2g31580.1 68415.m03858 expressed protein contains Pfam profi... 27 7.1
At5g13710.1 68418.m01596 sterol 24-C-methyltransferase, putative... 27 9.4
At3g16340.1 68416.m02066 ABC transporter family protein similar ... 27 9.4
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 54.8 bits (126), Expect = 4e-08
Identities = 28/87 (32%), Positives = 49/87 (56%), Gaps = 1/87 (1%)
Frame = +1
Query: 238 LEYIKELGVGAVWLSPIFKSPM-VDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKV 414
L IK+LG AV + I + FGY + NF+ +GT +D ++L+ KA+EL + V
Sbjct: 353 LPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVV 412
Query: 415 VLDLVPNHTSNESVWFQEALNGNEKYY 495
++D+V +H S ++ + +G + Y
Sbjct: 413 LMDIVHSHASKNTLDGLDMFDGTDGQY 439
>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-2) identical to starch
branching enzyme class II [Arabidopsis thaliana]
GI:726490
Length = 716
Score = 53.2 bits (122), Expect = 1e-07
Identities = 28/87 (32%), Positives = 49/87 (56%), Gaps = 1/87 (1%)
Frame = +1
Query: 238 LEYIKELGVGAVWLSPIFK-SPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKV 414
L IK+LG AV + I + S FGY + NF+ GT E+ ++L+ +A+EL + V
Sbjct: 229 LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKSLIDRAHELGLVV 288
Query: 415 VLDLVPNHTSNESVWFQEALNGNEKYY 495
++D+V +H S ++ +G + +Y
Sbjct: 289 LMDIVHSHASKNTLDGLNMFDGTDAHY 315
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 46.0 bits (104), Expect = 2e-05
Identities = 25/90 (27%), Positives = 48/90 (53%), Gaps = 2/90 (2%)
Frame = +1
Query: 238 LEYIKELGVGAVWLSPIFK-SPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKV 414
L ++K G A+ L + + GY + NF+ YGT +DF+ L+ +A+ L + V
Sbjct: 272 LPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLV 331
Query: 415 VLDLVPNHTSNESVWFQEALNG-NEKYYNY 501
LD+V ++ + + + +G N+ Y++Y
Sbjct: 332 FLDIVHSYAAADQMVGLSLFDGSNDCYFHY 361
>At1g69830.1 68414.m08034 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
SP|P17859 Alpha-amylase precursor (EC 3.2.1.1)
(1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo},
alpha-amylase [Malus x domestica] GI:7532799; contains
Pfam profile PF00128: Alpha amylase, catalytic domain
Length = 887
Score = 44.0 bits (99), Expect = 8e-05
Identities = 22/71 (30%), Positives = 40/71 (56%)
Frame = +1
Query: 226 ITSKLEYIKELGVGAVWLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELD 405
+ K + + LG +WL P +S + GY + Y ++ YGT+++ + +KK +++
Sbjct: 516 LQEKADELASLGFTVLWLPPPTESVSPE-GYMPKDLYNLNSRYGTIDELKDTVKKFHKVG 574
Query: 406 IKVVLDLVPNH 438
IKV+ D V NH
Sbjct: 575 IKVLGDAVLNH 585
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 42.7 bits (96), Expect = 2e-04
Identities = 23/81 (28%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
Frame = +1
Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYDIANFYEIHH-EYGTMEDFEALLK 387
G N + + ++ I G+ +WL P +S + GY Y+++ +YG+ + ++L+K
Sbjct: 42 GFYNSLHNSIDDIANAGITHLWLPPPSQSVAPE-GYLPGKLYDLNSSKYGSEAELKSLIK 100
Query: 388 KANELDIKVVLDLVPNHTSNE 450
N+ IK + D+V NH + E
Sbjct: 101 ALNQKGIKALADIVINHRTAE 121
>At4g09020.1 68417.m01489 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 3
[Solanum tuberosum] GI:27728149, isoamylase [Oryza
sativa] GI:3252794; contains Pfam profiles PF00128:
Alpha amylase catalytic domain, PF02922: Isoamylase
N-terminal domain
Length = 764
Score = 41.9 bits (94), Expect = 3e-04
Identities = 33/123 (26%), Positives = 62/123 (50%), Gaps = 27/123 (21%)
Frame = +1
Query: 157 ILYQIYPRSFADSDGDGI-----GDLNGITSKLEYIKELGVGAVWLSPIFK--------- 294
++Y++ R+F + G+ G G K+ ++++LG+ AV L P+F+
Sbjct: 237 VIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRR 296
Query: 295 -SP---MVD-FGYDIANFYEIHHEYGTME--------DFEALLKKANELDIKVVLDLVPN 435
+P MV+ +GY NF+ Y + E +F+ ++K + I+V+LD+V N
Sbjct: 297 SNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYN 356
Query: 436 HTS 444
HT+
Sbjct: 357 HTN 359
>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase from [Solanum
tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
[Hordeum vulgare] GI:21314275, [Oryza sativa]
GI:3252794; contains Pfam profiles PF00128: Alpha
amylase catalytic domain, PF02922: Isoamylase N-terminal
domain
Length = 783
Score = 40.7 bits (91), Expect = 7e-04
Identities = 32/124 (25%), Positives = 59/124 (47%), Gaps = 28/124 (22%)
Frame = +1
Query: 157 ILYQIYPRSFADSDGDGI---GDLNGITSKLEYIKELGVGAVWLSP-------------- 285
++Y+++ R F + I G G+ KL+++KELG+ + L P
Sbjct: 219 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 278
Query: 286 IFKSPMVDF-GYDIANFYEIHHEYGT----------MEDFEALLKKANELDIKVVLDLVP 432
I V+F GY F+ Y + + +F+ L+K+A++ I+V++D+V
Sbjct: 279 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 338
Query: 433 NHTS 444
NHT+
Sbjct: 339 NHTA 342
>At1g03310.2 68414.m00310 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 2
[Solanum tuberosum] GI:27728147, isoamylase from
[Triticum aestivum] GI:17932898, [Hordeum vulgare]
GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
profiles PF00128: Alpha amylase catalytic domain,
PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
gb|AA712462, gb|AA651230 and gb|N95932 come from this
gene
Length = 882
Score = 39.5 bits (88), Expect = 0.002
Identities = 25/86 (29%), Positives = 42/86 (48%), Gaps = 7/86 (8%)
Frame = +1
Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEA---- 378
G +G+ K+ ++K LG AV L PIF Y +F+ YG E+
Sbjct: 388 GTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNS 447
Query: 379 ---LLKKANELDIKVVLDLVPNHTSN 447
++KK + I+V+L++V HT++
Sbjct: 448 MKVMVKKLHSEGIEVLLEVVFTHTAD 473
>At1g03310.1 68414.m00309 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 2
[Solanum tuberosum] GI:27728147, isoamylase from
[Triticum aestivum] GI:17932898, [Hordeum vulgare]
GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
profiles PF00128: Alpha amylase catalytic domain,
PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
gb|AA712462, gb|AA651230 and gb|N95932 come from this
gene
Length = 882
Score = 39.5 bits (88), Expect = 0.002
Identities = 25/86 (29%), Positives = 42/86 (48%), Gaps = 7/86 (8%)
Frame = +1
Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEA---- 378
G +G+ K+ ++K LG AV L PIF Y +F+ YG E+
Sbjct: 388 GTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNS 447
Query: 379 ---LLKKANELDIKVVLDLVPNHTSN 447
++KK + I+V+L++V HT++
Sbjct: 448 MKVMVKKLHSEGIEVLLEVVFTHTAD 473
>At1g76130.1 68414.m08841 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative strong
similarity to alpha-amylase GI:7532799 from [Malus x
domestica];contains Pfam profile PF00128: Alpha amylase,
catalytic domain
Length = 413
Score = 37.5 bits (83), Expect = 0.007
Identities = 19/71 (26%), Positives = 37/71 (52%)
Frame = +1
Query: 226 ITSKLEYIKELGVGAVWLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELD 405
+ K+ I + G + WL P +S + GY + Y ++ YG+ ++LL+K +
Sbjct: 45 LDGKVPDIAKSGFTSAWLPPPSQS-LAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYK 103
Query: 406 IKVVLDLVPNH 438
++ + D+V NH
Sbjct: 104 VRAMADIVINH 114
>At1g07510.1 68414.m00804 FtsH protease, putative similar to
AAA-metalloprotease FtsH [Pisum sativum] GI:15021761;
contains Pfam profiles PF01434: Peptidase family M41,
PF00004: ATPase AAA family
Length = 813
Score = 32.3 bits (70), Expect = 0.25
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +1
Query: 334 YEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEAL 474
Y+ + G++E FE L++A E D VP +E++W+QE L
Sbjct: 222 YKYYFNIGSVESFEEKLEEAQEAIGVNSHDFVPVTYVSETIWYQELL 268
>At5g61760.1 68418.m07749 inositol polyphosphate 6-/3-/5-kinase 2b
(IPK2b) contains Pfam domain, PF03770: inositol
polyphosphate kinase
Length = 300
Score = 29.1 bits (62), Expect = 2.3
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = +1
Query: 85 SLLFVACSGIIIKNGEVQDWWETSILY 165
S ++ C+GI+ + E++DW+ET LY
Sbjct: 191 SEVYGGCNGILAQLLELKDWFETQTLY 217
>At2g23630.1 68415.m02819 multi-copper oxidase type I family protein
contains Pfam profile: PF00394 Multicopper oxidase
Length = 541
Score = 29.1 bits (62), Expect = 2.3
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +1
Query: 508 WEDGIIDENGNRQPPNNWLSHFR 576
W+DG++ N QP ++W HF+
Sbjct: 96 WQDGVLGTNCPIQPKSSWTYHFQ 118
>At5g09390.1 68418.m01088 CD2-binding protein-related similar to CD2
cytoplasmic domain binding protein [Homo sapiens]
GI:3983427
Length = 351
Score = 27.9 bits (59), Expect = 5.4
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = +1
Query: 202 DGIGDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYDIANFYEIHHEYG 357
+G D +G + +EY++E V WL I K+PM G AN E+ + G
Sbjct: 111 EGYFDADG--NFVEYVREKEVKDAWLDSIEKNPMY-MGRSAANDTEMDDDSG 159
>At4g37160.1 68417.m05261 multi-copper oxidase type I family protein
contains Pfam profile: PF00394 Multicopper oxidase
Length = 541
Score = 27.5 bits (58), Expect = 7.1
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +1
Query: 508 WEDGIIDENGNRQPPNNWLSHFR 576
W+DG++ N QP +NW F+
Sbjct: 97 WQDGVLGTNCPIQPNSNWTYQFQ 119
>At2g31580.1 68415.m03858 expressed protein contains Pfam profile:
PF04446 family of unknown function (DUF549)
Length = 567
Score = 27.5 bits (58), Expect = 7.1
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +1
Query: 322 IANFYEIHHEYGTMEDFEALLKKANEL 402
+ F +IH YG +++ +LKK +EL
Sbjct: 375 LEEFEDIHFAYGVSDEYSFVLKKESEL 401
>At5g13710.1 68418.m01596 sterol 24-C-methyltransferase, putative
similar to SP:P25087 Sterol 24-C-methyltransferase,
Delta(24)-sterol C- methyltransferase, Saccharomyces
cerevisiae
Length = 336
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +1
Query: 199 GDGIGDLNGITSKLEYIKELGVGAVWLSPIFK-SPM 303
GDG+ D+ T LE +K+ G +W + K SP+
Sbjct: 223 GDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPV 258
>At3g16340.1 68416.m02066 ABC transporter family protein similar to
PDR5-like ABC transporter GI:1514643 from [Spirodela
polyrhiza]; contains Pfam profile: PF00005 ABC
transporter
Length = 1416
Score = 27.1 bits (57), Expect = 9.4
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +1
Query: 79 LLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSF 186
L+ LL G I+ GE+ WW+ + Y + P ++
Sbjct: 668 LVILLLFLLGGFIVPRGEIPKWWKWA--YWVSPMAY 701
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,641,345
Number of Sequences: 28952
Number of extensions: 302847
Number of successful extensions: 788
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 765
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 787
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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