BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10j13f (565 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1296.02 |cox4||cytochrome c oxidase subunit IV|Schizosacchar... 62 4e-11 SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 27 2.5 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 27 2.5 SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizos... 26 3.3 SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 26 3.3 SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity prote... 25 5.8 SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subuni... 25 7.7 SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity f... 25 7.7 >SPAC1296.02 |cox4||cytochrome c oxidase subunit IV|Schizosaccharomyces pombe|chr 1|||Manual Length = 164 Score = 62.5 bits (145), Expect = 4e-11 Identities = 40/95 (42%), Positives = 54/95 (56%), Gaps = 3/95 (3%) Frame = +2 Query: 107 GYADKMMPDPLEHATGLERKELLALQAGNDDPFNMKVVKKS-AGTRENPTLVPSCFDARI 283 G D +P LE ATGLER ELL+ +G D F+MK + S GT +P +V S R Sbjct: 59 GGRDGEVPTDLEQATGLERYELLSELSGR-DAFDMKPLDASRKGTLTDPIMVTSLDPYRH 117 Query: 284 VGCICEEHAT-AITWLWLHKDQPRRC-ECGHWYKL 382 +GC + + W+ ++KD+ RRC ECG YKL Sbjct: 118 IGCTGSPSGSHNLIWMTVYKDKLRRCPECGSVYKL 152 >SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 26.6 bits (56), Expect = 2.5 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 6/68 (8%) Frame = -3 Query: 287 QQYEHQSRKVPELGFHGFLL-TSSLPSC*RGHHCRPEGP-----GVPSFPSQWHVPRDQA 126 QQ +HQ +K P+ G + T+S P+ H + +GP +PS P P A Sbjct: 270 QQKQHQQKKTPQSGSTPQMQNTTSQPTTHDTHPPKQQGPISDFRSIPSSPKTEGAP-SNA 328 Query: 125 SFCRHIPA 102 F +PA Sbjct: 329 QFRPSLPA 336 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 26.6 bits (56), Expect = 2.5 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Frame = -1 Query: 361 LATA-RLVLV*PKPGDGCSVFFTNASNNT 278 LA A LV+V +PG GCS F + +++T Sbjct: 183 LANAGELVMVLGQPGSGCSTFLRSVTSDT 211 >SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1649 Score = 26.2 bits (55), Expect = 3.3 Identities = 19/47 (40%), Positives = 25/47 (53%) Frame = -2 Query: 264 EGTRVGFSRVPADFFTTFMLKGSSLPA*RARSSFLSKPVACSKGSGI 124 +GT + FS +P FT K +S A +SFLS ACSK S + Sbjct: 591 DGTYL-FSYIPERLFTEKKPKNASKEI--AVTSFLSSHAACSKLSNV 634 >SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 26.2 bits (55), Expect = 3.3 Identities = 10/32 (31%), Positives = 14/32 (43%) Frame = -3 Query: 197 HHCRPEGPGVPSFPSQWHVPRDQASFCRHIPA 102 HH + P P+ Q HVP H+P+ Sbjct: 309 HHSHDDDPSSPAEKKQNHVPSPSEKIQDHVPS 340 >SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity protein kinase Mph1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 678 Score = 25.4 bits (53), Expect = 5.8 Identities = 21/83 (25%), Positives = 42/83 (50%) Frame = -3 Query: 458 MRFVSAVTLLDNKLKELLFKGVASQSICTNVHTRNGAVGPCVTKAR*WL*RVLHKCIQQY 279 ++ + A+ + N+ + F VA + V + G++ P VT + V+ +C+++ Sbjct: 532 LKMIQAIAAIPNEQYHIHFPEVALPA--NAVQEKEGSL-PGVTVGPDLM-DVMKRCLERD 587 Query: 278 EHQSRKVPELGFHGFLLTSSLPS 210 + + +PEL H FL + LPS Sbjct: 588 QRKRLTIPELLVHPFL--NPLPS 608 >SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subunit Orc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 707 Score = 25.0 bits (52), Expect = 7.7 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Frame = -2 Query: 345 WSLCNQSQVMAVACSSQMH-PTIRASKQEGTRVGFSRVPADFFTTFMLK 202 W S+++ VA ++ M P S + +R+G SRVP + +T L+ Sbjct: 473 WPSLPHSRLIVVAVANTMDLPERILSNRISSRLGLSRVPFEPYTHTQLE 521 >SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity factor complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 25.0 bits (52), Expect = 7.7 Identities = 15/73 (20%), Positives = 33/73 (45%) Frame = -3 Query: 473 AVITTMRFVSAVTLLDNKLKELLFKGVASQSICTNVHTRNGAVGPCVTKAR*WL*RVLHK 294 ++IT R + L +L + + A + + V + G C+ + W+ + + Sbjct: 251 SIITDFRINIIIVLGSERLYSSMKRKYADATWLSVVKVSSS--GGCIDREEEWIQQFQAR 308 Query: 293 CIQQYEHQSRKVP 255 CI+QY + ++P Sbjct: 309 CIKQYFYGDDRMP 321 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,476,391 Number of Sequences: 5004 Number of extensions: 54066 Number of successful extensions: 122 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 121 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 238029836 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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