Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10j09f
(599 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65560.1 68414.m07437 allyl alcohol dehydrogenase, putative s... 72 3e-13
At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 70 1e-12
At3g03080.1 68416.m00304 NADP-dependent oxidoreductase, putative... 67 1e-11
At5g16990.1 68418.m01990 NADP-dependent oxidoreductase, putative... 66 1e-11
At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative... 62 3e-10
At1g26320.1 68414.m03210 NADP-dependent oxidoreductase, putative... 60 1e-09
At5g16960.1 68418.m01987 NADP-dependent oxidoreductase, putative... 58 6e-09
At5g38000.1 68418.m04576 NADP-dependent oxidoreductase, putative... 56 1e-08
At5g37940.1 68418.m04570 NADP-dependent oxidoreductase, putative... 56 2e-08
At5g37980.1 68418.m04574 NADP-dependent oxidoreductase, putative... 55 4e-08
At3g59845.1 68416.m06678 NADP-dependent oxidoreductase, putative... 53 1e-07
At5g16980.1 68418.m01989 NADP-dependent oxidoreductase, putative... 40 0.002
At3g08490.1 68416.m00984 hypothetical protein 33 0.14
At5g60710.1 68418.m07618 zinc finger (C3HC4-type RING finger) fa... 29 2.4
At1g76120.2 68414.m08840 tRNA pseudouridine synthase family prot... 27 9.5
At1g76120.1 68414.m08839 tRNA pseudouridine synthase family prot... 27 9.5
>At1g65560.1 68414.m07437 allyl alcohol dehydrogenase, putative
similar to allyl alcohol dehydrogenase from Nicotiana
tabacum [gi:6692816]; similar to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 350
Score = 71.7 bits (168), Expect = 3e-13
Identities = 53/183 (28%), Positives = 85/183 (46%), Gaps = 13/183 (7%)
Frame = +3
Query: 90 NMVIAKKIIVVNPFVGVPKHNDFDI----VXXXXXXXXXXXFLAEAIYFSVDPYQRMKL- 254
++V KK+I+ N G+P D ++ FL + +Y S DPY R ++
Sbjct: 5 SVVENKKVILKNYVDGIPTETDMEVKLGETIELKAPKGSSCFLVKNLYLSCDPYMRGRMR 64
Query: 255 ---GNVYPCDMIGGQI-----ARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQ 410
G+ P + G +I ARVI+S +T Y G +V G GW +++ + + +
Sbjct: 65 DFHGSYLPPFVPGQRIEGFGLARVIDSDDTNYKPGDIVSGIIGWEEYSLLRSSDNLQLRN 124
Query: 411 KPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGETXXXXXXXXXXXXXXXQ 590
+L D +P+S LG+ G G TAY GF++IC PK G++ Q
Sbjct: 125 ----IQLDD--DIPLSYHLGLLGMAGFTAYAGFNEICCPKKGDSVFVSAACGAVGQLVGQ 178
Query: 591 IAK 599
+AK
Sbjct: 179 LAK 181
>At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 345
Score = 69.7 bits (163), Expect = 1e-12
Identities = 47/161 (29%), Positives = 74/161 (45%), Gaps = 15/161 (9%)
Frame = +3
Query: 105 KKIIVVNPFVGVPKHNDFDI---VXXXXXXXXXXXFLAEAIYFSVDPYQRMKLGNVYPCD 275
K++++ N G PK +DFD L + +Y S DPY R+++G P
Sbjct: 6 KQVVLKNYVSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYMRIRMGKPDPST 65
Query: 276 ------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQKPY 419
+ G ++RVIES + +Y G L+ G GW +++ P
Sbjct: 66 AALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMTHMH------ 119
Query: 420 LYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
+K+ + +P+S G+ G G TAY GF ++C PK GET
Sbjct: 120 -FKIQHTD-IPLSYYTGLLGMPGMTAYAGFYEVCSPKEGET 158
>At3g03080.1 68416.m00304 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana; similar to allyl alcohol
dehydrogenase [Nicotiana tabacum] GI:6692816
Length = 350
Score = 66.9 bits (156), Expect = 1e-11
Identities = 48/167 (28%), Positives = 77/167 (46%), Gaps = 16/167 (9%)
Frame = +3
Query: 90 NMVIAKKIIVVNPFV-GVPKHNDF---DIVXXXXXXXXXXXFLAEAIYFSVDPYQRMKLG 257
N +A K +++ +V G PK +D D L + +Y S DPY R+++G
Sbjct: 6 NATVANKQVILRDYVTGFPKESDLIFTDSTIDLKIPEGSKTVLVKNLYLSCDPYMRIRMG 65
Query: 258 NVYPCD------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKL 401
P + G +++VI+S + +Y G L+ G GW +++ P+
Sbjct: 66 KPDPGTAALAPHYIPGEPIYGFSVSKVIDSGHPDYKKGDLLWGLVGWGEYSLITPDFSH- 124
Query: 402 CQQKPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
YK+ + +P+S G+ G G TAY GF +IC PK GET
Sbjct: 125 -------YKIQHTD-VPLSYYTGLLGMPGMTAYAGFYEICSPKKGET 163
>At5g16990.1 68418.m01990 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 343
Score = 66.5 bits (155), Expect = 1e-11
Identities = 46/164 (28%), Positives = 72/164 (43%), Gaps = 14/164 (8%)
Frame = +3
Query: 93 MVIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGNV 263
M K++I + G PK +DF+ L + +Y S DPY R ++G
Sbjct: 1 MTTNKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKP 60
Query: 264 YPCDMI-----------GGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQ 410
P + G ++RVIES + +Y G L+ G GW +++ P
Sbjct: 61 DPSSALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITP-------- 112
Query: 411 KPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
+++ +P+S G+ G G TAY GF ++C PK GET
Sbjct: 113 MAHMHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGET 156
>At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative
(P1) identical to probable NADP-dependent oxidoreductase
P1, zeta-crystallin homolog [SP|Q39172][gi:886428],
Arabidopsis thaliana; similar to allyl alcohol
dehydrogenase [Nicotiana tabacum] GI:6692816; contains
Pfam profile PF00107: oxidoreductase, zinc-binding
dehydrogenase family
Length = 345
Score = 62.1 bits (144), Expect = 3e-10
Identities = 48/185 (25%), Positives = 76/185 (41%), Gaps = 16/185 (8%)
Frame = +3
Query: 93 MVIAKKIIVVNPFV-GVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGN 260
M K +++ +V G P +DFD L + +Y S DPY R+++G
Sbjct: 1 MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYMRIRMGK 60
Query: 261 VYPCDMIGGQ------------IARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLC 404
P Q ++R+IES + +Y G L+ G W +++ P
Sbjct: 61 PDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAH- 119
Query: 405 QQKPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGETXXXXXXXXXXXXXX 584
+K+ + +P+S G+ G G TAY GF ++C PK GET
Sbjct: 120 ------FKIQHTD-VPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLV 172
Query: 585 XQIAK 599
Q+AK
Sbjct: 173 GQLAK 177
>At1g26320.1 68414.m03210 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana; similar
to allyl alcohol dehydrogenase GI:9758497 from
[Arabidopsis thaliana]
Length = 351
Score = 59.7 bits (138), Expect = 1e-09
Identities = 46/164 (28%), Positives = 71/164 (43%), Gaps = 15/164 (9%)
Frame = +3
Query: 96 VIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGNVY 266
V K+II + G PK +D I L + +Y S DPY R+ +G
Sbjct: 9 VTNKQIIFPDYVTGFPKESDLKITTTTIDLRLPEGSTSVLVKNLYLSCDPYMRICMGKPD 68
Query: 267 PCD------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQ 410
P +IG +++VI+S + +Y G L+ G GW +++
Sbjct: 69 PLSSSLVPPYKTGVPIIGLGVSKVIDSGHPDYKKGDLLWGLVGWEEYSVITLTT------ 122
Query: 411 KPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
Y + + +P+S G+ G G TAY GF ++C PK GET
Sbjct: 123 --YSHFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGET 164
>At5g16960.1 68418.m01987 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana
Length = 346
Score = 57.6 bits (133), Expect = 6e-09
Identities = 43/163 (26%), Positives = 71/163 (43%), Gaps = 15/163 (9%)
Frame = +3
Query: 99 IAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXXF---LAEAIYFSVDPYQRMKLGNVYP 269
I K++++++ G K +D I L + +Y S DPY R ++ P
Sbjct: 5 INKQVVLLDYVTGFAKESDLVITSTTIDLRVLKGSMTALVKNLYLSCDPYMRNRMRKPDP 64
Query: 270 CDMIGGQ------------IARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQK 413
Q +++VI+S +++Y G L+ G GW +++ P
Sbjct: 65 LSPATAQSFTPGKPISGFGVSKVIDSGHSDYEEGDLIWGAVGWEEYSVITPI-------- 116
Query: 414 PYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
P L+ + P+S G+ G G TAY GF +IC PK G+T
Sbjct: 117 PNLHFKIHHTNFPLSYYTGLLGMPGMTAYVGFYEICTPKKGDT 159
>At5g38000.1 68418.m04576 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana
Length = 353
Score = 56.4 bits (130), Expect = 1e-08
Identities = 44/161 (27%), Positives = 69/161 (42%), Gaps = 16/161 (9%)
Frame = +3
Query: 108 KIIVVNPFVGVPKHNDFDIVXXXXXXXXXXXFLA---EAIYFSVDPYQRMKLGNVYPCD- 275
++I N G PK +D I +A + +Y S DP+ R ++G P
Sbjct: 14 QVIFRNYVNGFPKESDLYIAASTVDLRVPPGSMAVLVKNLYLSCDPHSRTRMGKPDPSSP 73
Query: 276 ------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQKPY 419
+ G +A+ I+S + Y G L+ G GW +++ P
Sbjct: 74 ASMAHAFTIGKPISGFGVAKAIDSGHPNYKTGDLLWGRVGWEEYSVITPTPSSH------ 127
Query: 420 LYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
+K+ + +P+S G+ G G TAY GF +IC PK GET
Sbjct: 128 -FKIHHTD-VPLSFYTGLLGIPGLTAYIGFYEICSPKKGET 166
>At5g37940.1 68418.m04570 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428],
Arabidopsis thaliana
Length = 353
Score = 55.6 bits (128), Expect = 2e-08
Identities = 43/160 (26%), Positives = 69/160 (43%), Gaps = 17/160 (10%)
Frame = +3
Query: 114 IVVNPFV-GVPKHNDFDIVXXXXXXXXXXXFLA---EAIYFSVDPYQRMKLGNVYPCDMI 281
+++ +V G PK +D I +A + +Y S DP+ R ++G P
Sbjct: 15 VILRDYVSGFPKQSDLYIATTTIDLRVPPGSMAVLVKNLYLSCDPHSRTRMGKPDPSSQA 74
Query: 282 -------------GGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQKPYL 422
G +A+ I+S + Y G L+ G GW +++ P
Sbjct: 75 SMAHAFTIGKPISGFGVAKAIDSGHPNYKTGDLLWGRVGWEEYSVITPTPSSH------- 127
Query: 423 YKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
+K+ + +P+S G+ G G TAY GF +IC PK GET
Sbjct: 128 FKIHHTD-VPLSFYTGLLGIPGLTAYVGFYEICSPKKGET 166
>At5g37980.1 68418.m04574 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana
Length = 353
Score = 54.8 bits (126), Expect = 4e-08
Identities = 43/161 (26%), Positives = 70/161 (43%), Gaps = 16/161 (9%)
Frame = +3
Query: 108 KIIVVNPFVGVPKHNDFDIVXXXXXXXXXXXFLA---EAIYFSVDPYQRMKLGNVYPCD- 275
++I+ N G PK +D I +A + +Y S DP+ R+++ P
Sbjct: 14 QVILRNYVSGFPKESDLYIAATTVDLRVPPGSMAVLVKNLYLSCDPFSRIRMEKPDPSSP 73
Query: 276 ------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQKPY 419
+ G +A+ I+S + Y G L+ G GW +++ P
Sbjct: 74 ASVARAYSIGKPISGFGVAKAIDSCHPNYKTGDLLWGRVGWEEYSVITPTPSSH------ 127
Query: 420 LYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
+K+ + +P+S G+ G G TAY GF +IC PK GET
Sbjct: 128 -FKIHHTD-VPLSFYTGLLGIPGLTAYVGFYEICSPKKGET 166
>At3g59845.1 68416.m06678 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana; allyl
alcohol dehydrogenase - Nicotiana tabacum, EMBL:AB036735
Length = 346
Score = 53.2 bits (122), Expect = 1e-07
Identities = 42/170 (24%), Positives = 74/170 (43%), Gaps = 17/170 (10%)
Frame = +3
Query: 84 VENMVIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX-----FLAEAIYFSVDPYQRM 248
+E V K++I+ N G+PK +D I+ + +Y S DPY +
Sbjct: 1 METTVKNKQVILRNYVKGIPKESDLMIITASDPVELKVKPGSAAVMVRNLYLSCDPYMGI 60
Query: 249 KLGNVYPCDM------IGGQ------IARVIESKNTEYPVGTLVMGHFGWRTHTITKPEN 392
+ P + I G+ +++VI+S + + G ++ G W + P
Sbjct: 61 LMREPTPSTLALLDAFIPGKPIAGIGVSQVIDSDDPSFTKGDMIWGIVNWEEFSTINPAA 120
Query: 393 EKLCQQKPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
++K+ ++P+S G+ G +G TAY GF +IC PK G+T
Sbjct: 121 ---------IFKIDVNINVPLSYYTGILGMIGLTAYAGFFEICSPKKGDT 161
>At5g16980.1 68418.m01989 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 239
Score = 39.5 bits (88), Expect = 0.002
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = +3
Query: 447 LPVSLGLGVCGRVGNTAYFGFSQICQPKPGET 542
+P+S G+ G G TAY GF +IC PK GET
Sbjct: 21 VPLSYYTGLLGMPGMTAYVGFYEICSPKKGET 52
>At3g08490.1 68416.m00984 hypothetical protein
Length = 271
Score = 33.1 bits (72), Expect = 0.14
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -3
Query: 300 LWQFVHQSYHKDIHCLVSFFDKDRH*SILLLLETHFLV 187
+W ++ YH + CL+ DK+RH + ++ E LV
Sbjct: 233 VWNIINPKYHHKVECLLLLADKERHSHVTMIREKCRLV 270
>At5g60710.1 68418.m07618 zinc finger (C3HC4-type RING finger)
family protein low similarity to SP|P97280
Inter-alpha-trypsin inhibitor heavy chain H3 precursor
{Mesocricetus auratus}; contains Pfam profiles PF00097:
Zinc finger, C3HC4 type (RING finger), PF00092: von
Willebrand factor type A domain
Length = 704
Score = 29.1 bits (62), Expect = 2.4
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +3
Query: 294 ARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQKPYLYKLPDFEHLPV-SLGLG 470
ARV+ + + PV ++V+ G T+T+T P + K L K + +PV + G G
Sbjct: 337 ARVLIDRRFKNPVSSIVLLSDGQDTYTMTSPNGSRGTDYKALLPKEINGNRIPVHAFGFG 396
>At1g76120.2 68414.m08840 tRNA pseudouridine synthase family protein
similar to SP|Q9Y606 tRNA pseudouridine synthase A (EC
4.2.1.70) (Uracil hydrolyase) {Homo sapiens}; contains
Pfam profile PF01416: tRNA pseudouridine synthase
Length = 356
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +3
Query: 354 FGWRTHTITKPENEKLCQQKPYLYKLPDFEHLPVS 458
FG++ H ++K C ++ Y+Y LP F P+S
Sbjct: 117 FGYK-HVTPSFSSKKFCDRRRYVYLLPVFALDPIS 150
>At1g76120.1 68414.m08839 tRNA pseudouridine synthase family protein
similar to SP|Q9Y606 tRNA pseudouridine synthase A (EC
4.2.1.70) (Uracil hydrolyase) {Homo sapiens}; contains
Pfam profile PF01416: tRNA pseudouridine synthase
Length = 463
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +3
Query: 354 FGWRTHTITKPENEKLCQQKPYLYKLPDFEHLPVS 458
FG++ H ++K C ++ Y+Y LP F P+S
Sbjct: 117 FGYK-HVTPSFSSKKFCDRRRYVYLLPVFALDPIS 150
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,791,018
Number of Sequences: 28952
Number of extensions: 254360
Number of successful extensions: 625
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 597
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 613
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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