Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10j05f
(589 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21540.1 68417.m03112 diacylglycerol kinase family protein co... 65 3e-11
At5g23450.2 68418.m02752 diacylglycerol kinase family protein co... 60 8e-10
At5g23450.1 68418.m02751 diacylglycerol kinase family protein co... 60 8e-10
At2g18730.1 68415.m02181 diacylglycerol kinase, putative contain... 40 0.001
At4g30340.1 68417.m04312 diacylglycerol kinase family protein co... 39 0.003
At2g46090.1 68415.m05733 diacylglycerol kinase family protein co... 37 0.009
At5g63770.1 68418.m08004 diacylglycerol kinase, putative similar... 37 0.011
At5g57690.1 68418.m07211 diacylglycerol kinase, putative contain... 36 0.015
At1g73890.1 68414.m08558 protease inhibitor/seed storage/lipid t... 35 0.046
At4g28130.1 68417.m04033 diacylglycerol kinase accessory domain-... 32 0.33
At4g14760.1 68417.m02271 M protein repeat-containing protein con... 29 2.3
At3g04710.1 68416.m00505 ankyrin repeat family protein contains ... 29 3.0
At1g25450.1 68414.m03160 very-long-chain fatty acid condensing e... 29 3.0
At3g14690.1 68416.m01858 cytochrome P450, putative similar to GB... 28 4.0
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 28 4.0
At2g20900.3 68415.m02465 diacylglycerol kinase, putative contain... 28 4.0
At2g20900.2 68415.m02464 diacylglycerol kinase, putative contain... 28 4.0
At2g20900.1 68415.m02463 diacylglycerol kinase, putative contain... 28 4.0
At2g30340.1 68415.m03692 LOB domain protein 13 / lateral organ b... 28 5.3
At3g56130.2 68416.m06239 biotin/lipoyl attachment domain-contain... 27 7.0
At3g56130.1 68416.m06238 biotin/lipoyl attachment domain-contain... 27 7.0
At1g14350.1 68414.m01701 myb family transcription factor (MYB124... 27 7.0
At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-rel... 27 9.3
At2g19660.1 68415.m02297 DC1 domain-containing protein contains ... 27 9.3
At1g22760.1 68414.m02844 polyadenylate-binding protein 3 (PABP3) 27 9.3
>At4g21540.1 68417.m03112 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase catalytic
domain
Length = 1240
Score = 65.3 bits (152), Expect = 3e-11
Identities = 35/103 (33%), Positives = 58/103 (56%), Gaps = 4/103 (3%)
Frame = +2
Query: 236 PTVITVILNPVANKRKAKQEFEKYCEPXXXXXXXQVDVVQTASEGNAKEIVETLRGT--E 409
P + V +NP K+ A++ F++ +P Q+++ +T + +AKEIV ++ + +
Sbjct: 907 PKKLLVFVNPFGGKKTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYD 966
Query: 410 AIIVAGGDGTLSETVTGLLRRND--QANMFPVGVLPLGRTNSI 532
I+ GDG L E V GLL R D A P+G++P GRT S+
Sbjct: 967 GIVCVSGDGILVEVVNGLLEREDWKTAIKLPIGMVPAGRTRSL 1009
Score = 60.5 bits (140), Expect = 8e-10
Identities = 34/107 (31%), Positives = 57/107 (53%), Gaps = 4/107 (3%)
Frame = +2
Query: 236 PTVITVILNPVANKRKAKQEFEKYCEPXXXXXXXQVDVVQTASEGNAKEIVETLRGT--E 409
P + V +NP K+ A++ F K +P Q+++ +T + +AKE V+++ + +
Sbjct: 377 PKRLLVFVNPFGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYD 436
Query: 410 AIIVAGGDGTLSETVTGLLRRNDQAN--MFPVGVLPLGRTNSIGNTL 544
I+ GDG L E V GLL R D N P+G++P G N + +L
Sbjct: 437 GIVCVSGDGILVEVVNGLLERADWRNALKLPIGMVPAGTGNGMIKSL 483
>At5g23450.2 68418.m02752 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 763
Score = 60.5 bits (140), Expect = 8e-10
Identities = 32/99 (32%), Positives = 56/99 (56%), Gaps = 5/99 (5%)
Frame = +2
Query: 251 VILNPVANKRKAKQEFEKYCEPXXXXXXXQVDVVQTASEGNAKEIVETLR---GTEAIIV 421
VILNP + ++ + F EP +++VV+T G+A+E+ T+ ++ II
Sbjct: 252 VILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVDINLCSDGIIC 311
Query: 422 AGGDGTLSETVTGLLRRND--QANMFPVGVLPLGRTNSI 532
GGDG ++E + GLL R++ + P+G++P G NS+
Sbjct: 312 VGGDGIINEVLNGLLTRSNPKEGVSIPIGIVPAGSDNSL 350
>At5g23450.1 68418.m02751 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 763
Score = 60.5 bits (140), Expect = 8e-10
Identities = 32/99 (32%), Positives = 56/99 (56%), Gaps = 5/99 (5%)
Frame = +2
Query: 251 VILNPVANKRKAKQEFEKYCEPXXXXXXXQVDVVQTASEGNAKEIVETLR---GTEAIIV 421
VILNP + ++ + F EP +++VV+T G+A+E+ T+ ++ II
Sbjct: 252 VILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVDINLCSDGIIC 311
Query: 422 AGGDGTLSETVTGLLRRND--QANMFPVGVLPLGRTNSI 532
GGDG ++E + GLL R++ + P+G++P G NS+
Sbjct: 312 VGGDGIINEVLNGLLTRSNPKEGVSIPIGIVPAGSDNSL 350
>At2g18730.1 68415.m02181 diacylglycerol kinase, putative contains
INTERPRO domain, IPR001206, DAG-kinase catalytic domain
Length = 488
Score = 39.9 bits (89), Expect = 0.001
Identities = 23/65 (35%), Positives = 38/65 (58%), Gaps = 2/65 (3%)
Frame = +2
Query: 353 QTASEGNAKEIVETLRGTEAIIVAGGDGTLSETV--TGLLRRNDQANMFPVGVLPLGRTN 526
+ A+EG+ E + R I+VAGGDGT+ + G L ++ ++ + PVGV+PLG N
Sbjct: 142 KVAAEGD--ECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPLGTGN 199
Query: 527 SIGNT 541
+ +
Sbjct: 200 DLSRS 204
>At4g30340.1 68417.m04312 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 374
Score = 38.7 bits (86), Expect = 0.003
Identities = 23/69 (33%), Positives = 40/69 (57%), Gaps = 2/69 (2%)
Frame = +2
Query: 341 VDVVQTASEGNAKEIVETLRGTEAIIVAGGDGTLSETV--TGLLRRNDQANMFPVGVLPL 514
+D + + A+E E +R I+VAGGDGT+ + G L ++ ++++ PVGV+PL
Sbjct: 143 LDTLAAKGDECARECREKIR----IMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPL 198
Query: 515 GRTNSIGNT 541
G N + +
Sbjct: 199 GTGNDLSRS 207
>At2g46090.1 68415.m05733 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 364
Score = 37.1 bits (82), Expect = 0.009
Identities = 36/111 (32%), Positives = 50/111 (45%), Gaps = 5/111 (4%)
Frame = +2
Query: 245 ITVILNPV-ANKRKAKQEFEKYCEPXXXXXXXQVDVVQTASEG--NAKEIV-ETLR-GTE 409
+ ++NP AN R AK E++K +V + + G +A +I E +R G +
Sbjct: 54 LVFVVNPQGANGRTAK-EWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDGAD 112
Query: 410 AIIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLGRTNSIGNTLFPNGKG 562
A+I GGDGTL E V G PVG L + S L P G G
Sbjct: 113 AVIAVGGDGTLHEVVNGFFWEGK-----PVGYLSGEASRSTALGLIPLGTG 158
>At5g63770.1 68418.m08004 diacylglycerol kinase, putative similar to
diacylglycerol kinase, theta (diglyceride kinase, DGK-
theta, DAG kinase theta). [Homo sapiens]
SWISS-PROT:P52824
Length = 712
Score = 36.7 bits (81), Expect = 0.011
Identities = 19/54 (35%), Positives = 32/54 (59%)
Frame = +2
Query: 413 IIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLGRTNSIGNTLFPNGKGVQKV 574
++V GGDGT++ + + +RN ++ PV +LPLG N + L G+G+ V
Sbjct: 396 VLVCGGDGTVAWVLDAIEKRNFESPP-PVAILPLGTGNDLSRVL-QWGRGISVV 447
>At5g57690.1 68418.m07211 diacylglycerol kinase, putative contains
INTERPRO domain, IPR001206, DAG-kinase catalytic domain
Length = 498
Score = 36.3 bits (80), Expect = 0.015
Identities = 25/65 (38%), Positives = 37/65 (56%), Gaps = 4/65 (6%)
Frame = +2
Query: 359 ASEGN--AKEIVETLRGTEAIIVAGGDGTLSETV--TGLLRRNDQANMFPVGVLPLGRTN 526
AS G+ AKEI E +R I+VAGGDGT+ + G L ++ + PV ++PLG N
Sbjct: 143 ASRGDECAKEIREKMR----IVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGN 198
Query: 527 SIGNT 541
+ +
Sbjct: 199 DLSRS 203
>At1g73890.1 68414.m08558 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 193
Score = 34.7 bits (76), Expect = 0.046
Identities = 19/52 (36%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
Frame = -2
Query: 459 PVTVSDKVPSPPATIIASVPLNVSTISFAFPSEAVC-TTSTCKPPNNSNGSQ 307
P+ + + PP + ++P N ST S +FP EA ++S PP++S GSQ
Sbjct: 85 PINQTLALQLPP---LCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQ 133
>At4g28130.1 68417.m04033 diacylglycerol kinase accessory
domain-containing protein similar to diacylglycerol
kinase [Lycopersicon esculentum] GI:10798892; contains
Pfam profile PF00609: Diacylglycerol kinase accessory
domain (presumed)
Length = 466
Score = 31.9 bits (69), Expect = 0.33
Identities = 17/50 (34%), Positives = 29/50 (58%)
Frame = +2
Query: 383 IVETLRGTEAIIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLGRTNSI 532
+ +R IIVAGGDGT + + G++ + +N P+ +PLG N++
Sbjct: 104 LASKIRDKLKIIVAGGDGT-AGWLLGVVSDLNLSNPPPIATVPLGTGNNL 152
>At4g14760.1 68417.m02271 M protein repeat-containing protein
contains Pfam profile: PF02370 M protein repeat
Length = 1676
Score = 29.1 bits (62), Expect = 2.3
Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = +3
Query: 54 KE*RSLPKQYET----IGRNQSLVQSLYTTLVRLQKKNMKLIYLCEQHVKRLQFTAKL 215
+E RS ++YE + Q + L + L++KN L+ C++HV+ +F+ KL
Sbjct: 767 EECRSRKREYEDELDRVVNKQVEIFILQKLIEDLEQKNFSLLIECQKHVEASEFSEKL 824
>At3g04710.1 68416.m00505 ankyrin repeat family protein contains
Pfam profile: PF00023 ankyrin repeat
Length = 456
Score = 28.7 bits (61), Expect = 3.0
Identities = 21/67 (31%), Positives = 31/67 (46%)
Frame = +2
Query: 338 QVDVVQTASEGNAKEIVETLRGTEAIIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLG 517
Q + V+ E NA ET ++ A G+LS + L++ +AN+F G PL
Sbjct: 166 QKNAVEVLLEHNANPNAETEDNITPLLSAVAAGSLS-CLELLVKAGAKANVFAGGATPLH 224
Query: 518 RTNSIGN 538
IGN
Sbjct: 225 IAADIGN 231
>At1g25450.1 68414.m03160 very-long-chain fatty acid condensing
enzyme, putative nearly identical to fatty acid
condensing enzyme CUT1 GI:5001734 from [Arabidopsis
thaliana]
Length = 492
Score = 28.7 bits (61), Expect = 3.0
Identities = 21/71 (29%), Positives = 28/71 (39%)
Frame = -2
Query: 315 GSQYFSNSCFAFLLLATGLRITVMTVGFLSMGRRASP*IAASLHAARISILISYFSFAVA 136
G QY N+ LL+ I + + L MG + SLH + IL S F
Sbjct: 16 GYQYLINNFLTLLLIPV---IATVAIELLRMGPEEILSVLNSLHFELLHILCSSFLIIFV 72
Query: 135 LA*YIATAPRT 103
Y + PRT
Sbjct: 73 STVYFMSKPRT 83
>At3g14690.1 68416.m01858 cytochrome P450, putative similar to
GB:Q05047 from [Catharanthus roseus]
Length = 512
Score = 28.3 bits (60), Expect = 4.0
Identities = 23/64 (35%), Positives = 29/64 (45%)
Frame = +3
Query: 72 PKQYETIGRNQSLVQSLYTTLVRLQKKNMKLIYLCEQHVKRLQFTAKLSCPSKETPQS*Q 251
PK E R Q L + YT LV KKN + L E K L+ T +S + P Q
Sbjct: 32 PKMLEHYLRRQGLAGTPYTPLVGDLKKNFTM--LSEARSKPLKLTDDIS--PRVVPYPLQ 87
Query: 252 LFST 263
+F T
Sbjct: 88 MFKT 91
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 28.3 bits (60), Expect = 4.0
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = -2
Query: 435 PSPPATIIASVPLNVSTISFAFPSEAVCTTSTCKPPNNSNGSQYFSNSCFA 283
P PP+ + S P+N ++ S P + + PP+ + ++ + C+A
Sbjct: 62 PFPPSLTVESTPVNANSASLTTPL-MIDPLRSWNPPDEQSRNESIARFCYA 111
>At2g20900.3 68415.m02465 diacylglycerol kinase, putative contains
INTERPRO domain, IPR001206, DAG-kinase catalytic domain
Length = 491
Score = 28.3 bits (60), Expect = 4.0
Identities = 19/53 (35%), Positives = 32/53 (60%)
Frame = +2
Query: 374 AKEIVETLRGTEAIIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLGRTNSI 532
A++I E L+ IIVAGGDGT + + G++ ++ P+ +PLG N++
Sbjct: 98 ARQIREKLK----IIVAGGDGT-AGWLLGVVCDLKLSHPPPIATVPLGTGNNL 145
>At2g20900.2 68415.m02464 diacylglycerol kinase, putative contains
INTERPRO domain, IPR001206, DAG-kinase catalytic domain
Length = 491
Score = 28.3 bits (60), Expect = 4.0
Identities = 19/53 (35%), Positives = 32/53 (60%)
Frame = +2
Query: 374 AKEIVETLRGTEAIIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLGRTNSI 532
A++I E L+ IIVAGGDGT + + G++ ++ P+ +PLG N++
Sbjct: 98 ARQIREKLK----IIVAGGDGT-AGWLLGVVCDLKLSHPPPIATVPLGTGNNL 145
>At2g20900.1 68415.m02463 diacylglycerol kinase, putative contains
INTERPRO domain, IPR001206, DAG-kinase catalytic domain
Length = 509
Score = 28.3 bits (60), Expect = 4.0
Identities = 19/53 (35%), Positives = 32/53 (60%)
Frame = +2
Query: 374 AKEIVETLRGTEAIIVAGGDGTLSETVTGLLRRNDQANMFPVGVLPLGRTNSI 532
A++I E L+ IIVAGGDGT + + G++ ++ P+ +PLG N++
Sbjct: 98 ARQIREKLK----IIVAGGDGT-AGWLLGVVCDLKLSHPPPIATVPLGTGNNL 145
>At2g30340.1 68415.m03692 LOB domain protein 13 / lateral organ
boundaries domain protein 13 (LBD13) identical to LOB
DOMAIN 13 [Arabidopsis thaliana] GI:17227158 SP|Q9AT61
Length = 268
Score = 27.9 bits (59), Expect = 5.3
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = -2
Query: 459 PVTVSDKVPSPPATIIASVPLNVSTISFAFPSEAVCTTSTCKPPNNSNGSQYFSNS 292
P + P+PP T S+P +S + S + T S PP +S +SNS
Sbjct: 204 PPRLLSSQPAPPPTPPVSLPSPSMVVSSSSSSNSSATNSMYNPPPSSTAG--YSNS 257
>At3g56130.2 68416.m06239 biotin/lipoyl attachment domain-containing
protein low similarity to SP|Q06881 Biotin carboxyl
carrier protein of acetyl-CoA carboxylase (BCCP)
{Anabaena sp.}; contains Pfam profile PF00364:
Biotin-requiring enzyme
Length = 205
Score = 27.5 bits (58), Expect = 7.0
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -2
Query: 462 NPVTVSDKVPSPPATIIASVPLNVSTISFAFPSEAVCTTSTCKPPNNSN 316
NP+ V+D P+ A I S P+N S S PS+A ++ P N++
Sbjct: 52 NPIPVADISPTV-APPIPSEPMNKSASSAPSPSQAKPSSEKVSPFKNTS 99
>At3g56130.1 68416.m06238 biotin/lipoyl attachment domain-containing
protein low similarity to SP|Q06881 Biotin carboxyl
carrier protein of acetyl-CoA carboxylase (BCCP)
{Anabaena sp.}; contains Pfam profile PF00364:
Biotin-requiring enzyme
Length = 281
Score = 27.5 bits (58), Expect = 7.0
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -2
Query: 462 NPVTVSDKVPSPPATIIASVPLNVSTISFAFPSEAVCTTSTCKPPNNSN 316
NP+ V+D P+ A I S P+N S S PS+A ++ P N++
Sbjct: 128 NPIPVADISPTV-APPIPSEPMNKSASSAPSPSQAKPSSEKVSPFKNTS 175
>At1g14350.1 68414.m01701 myb family transcription factor (MYB124)
contains PFAM profile: PF00249 myb-like DNA binding
domain
Length = 436
Score = 27.5 bits (58), Expect = 7.0
Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +3
Query: 93 GRNQSLVQSLYTTLVRLQKKNMKLIYLCEQHVKRLQFTAKLSCPSK---ETP 239
GR + V++ +TTL + + K+ + + KR+ F +S P K ETP
Sbjct: 108 GRTDNAVKNRFTTLCKKRAKHEAMTKDSNSNTKRMLFLDGISTPRKSENETP 159
>At2g45540.1 68415.m05663 WD-40 repeat family protein /
beige-related contains Pfam PF02138: Beige/BEACH domain;
contains Pfam PF00400: WD domain, G-beta repeat (3
copies)
Length = 2946
Score = 27.1 bits (57), Expect = 9.3
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = -2
Query: 459 PVTVSDKVPSPPATIIASVPLNVSTISFAFPSEAVCTTSTCKPPNN 322
P+TVS + ++PL++ST++ A P + + + P NN
Sbjct: 689 PLTVSSVHKDSLEPCLGNLPLSLSTVTLAAPVFRIMSVAIQHPGNN 734
>At2g19660.1 68415.m02297 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 662
Score = 27.1 bits (57), Expect = 9.3
Identities = 16/41 (39%), Positives = 19/41 (46%)
Frame = -1
Query: 301 LKFLFCFPLVGYRVENNCYDCGVSFDGQESFAVNCSLFTCC 179
LK L C Y +N C CG FDG+ C+ FT C
Sbjct: 183 LKLLRCEEAPDY-ADNKCLLCGNKFDGRIHHCDVCN-FTIC 221
>At1g22760.1 68414.m02844 polyadenylate-binding protein 3 (PABP3)
Length = 660
Score = 27.1 bits (57), Expect = 9.3
Identities = 18/47 (38%), Positives = 22/47 (46%)
Frame = -2
Query: 459 PVTVSDKVPSPPATIIASVPLNVSTISFAFPSEAVCTTSTCKPPNNS 319
P T+ D+VPSP A VP +S PS V T PN+S
Sbjct: 11 PATMVDQVPSPTAQTSVQVP-----VSIPLPSPVVVADQT--HPNSS 50
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,743,369
Number of Sequences: 28952
Number of extensions: 233149
Number of successful extensions: 825
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 795
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 818
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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