Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10i21f
         (663 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g42050.1 68416.m04311 vacuolar ATP synthase subunit H family ...    66   3e-11
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-...    37   0.010
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-...    37   0.010
At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to SP...    33   0.22 
At5g02100.1 68418.m00131 oxysterol-binding family protein simila...    28   4.8  
At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein...    28   4.8  
At4g31570.1 68417.m04483 expressed protein                             27   8.4  

>At3g42050.1 68416.m04311 vacuolar ATP synthase subunit H family
           protein identical to probable vacuolar ATP synthase
           subunit H (EC 3.6.3.14)(V-ATPase H subunit) (Vacuolar
           proton pump H subunit) (Vacuolar proton pump subunit
           SFD) SP:Q9LX65 from [Arabidopsis thaliana]; contains
           Pfam PF03224: V-ATPase subunit H
          Length = 441

 Score = 65.7 bits (153), Expect = 3e-11
 Identities = 30/133 (22%), Positives = 77/133 (57%), Gaps = 4/133 (3%)
 Frame = +2

Query: 161 QIRASEIRQTRINWQSYLQSQMITQRDHDFIVNLDQRGQK---DLPDKNPDACAEVFLNL 331
           ++   ++ +  I W++Y+ +++++ +    +   D++ +     L D++  A   +F+++
Sbjct: 5   ELSIEQVLKRDIPWETYMNTKLVSAKGLQLLRRYDKKPESARAQLLDEDGPAYVHLFVSI 64

Query: 332 LTHISKDHTIQYILVLIDDILSEDKSRVKIFRETKFSG-NVWQPFLNLLNRQDEFVQHMT 508
           L  I K+ T++Y+L LI ++LS + +R ++F +   +  + ++PFL LL + + F+Q  +
Sbjct: 65  LRDIFKEETVEYVLALIYEMLSANPTRARLFHDESLANEDTYEPFLRLLWKGNWFIQEKS 124

Query: 509 ARIIAKLACWHPQ 547
            +I+A +    P+
Sbjct: 125 CKILAWIISARPK 137


>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
           beta-coat protein, putative / beta-COP, putative similar
           to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
           from {Rattus norvegicus} SP|P23514, {Mus musculus}
           SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 948

 Score = 37.1 bits (82), Expect = 0.010
 Identities = 19/80 (23%), Positives = 45/80 (56%), Gaps = 2/80 (2%)
 Frame = +2

Query: 296 NPDACAEVFLNLLTHI--SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLN 469
           N +   ++F+ ++ ++  S+DHTIQ +L+L  +++ +  S+ K+  E      + Q   N
Sbjct: 47  NGETIPQLFITIIRYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMIL---ICQNLRN 103

Query: 470 LLNRQDEFVQHMTARIIAKL 529
            L   +E+++ +T R + ++
Sbjct: 104 NLQHPNEYIRGVTLRFLCRM 123


>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
           beta-coat protein, putative / beta-COP, putative similar
           to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
           from {Rattus norvegicus} SP|P23514, {Mus musculus}
           SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 971

 Score = 37.1 bits (82), Expect = 0.010
 Identities = 19/80 (23%), Positives = 45/80 (56%), Gaps = 2/80 (2%)
 Frame = +2

Query: 296 NPDACAEVFLNLLTHI--SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLN 469
           N +   ++F+ ++ ++  S+DHTIQ +L+L  +++ +  S+ K+  E      + Q   N
Sbjct: 70  NGETIPQLFITIIRYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMIL---ICQNLRN 126

Query: 470 LLNRQDEFVQHMTARIIAKL 529
            L   +E+++ +T R + ++
Sbjct: 127 NLQHPNEYIRGVTLRFLCRM 146


>At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to
           SP|Q9UPM8 Adapter-related protein complex 4 epsilon 1
           subunit (Epsilon subunit of AP-4) (AP-4 adapter complex
           epsilon subunit) {Homo sapiens}; contains Pfam profile:
           PF01602 Adaptin N terminal region
          Length = 938

 Score = 32.7 bits (71), Expect = 0.22
 Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
 Frame = +2

Query: 209 YLQSQMITQRDHDFIVNLDQRGQKDL-PDKNPDACAEVFLNLLTHISKDHTIQYILVLID 385
           YL   +    DHD I+ +    QKDL  D     CA   LN +  +  + TI  +L  + 
Sbjct: 106 YLAVTLFLNEDHDLIILIVNTIQKDLRSDNYLVVCAA--LNAICRLINEETIPAVLPQVV 163

Query: 386 DILSEDKSRVK 418
           ++L+  K  V+
Sbjct: 164 ELLNHQKEAVR 174


>At5g02100.1 68418.m00131 oxysterol-binding family protein similar
           to SWH1 [Saccharomyces cerevisiae] GI:402658; contains
           Pfam profile PF01237: Oxysterol-binding protein
          Length = 453

 Score = 28.3 bits (60), Expect = 4.8
 Identities = 11/33 (33%), Positives = 15/33 (45%)
 Frame = +2

Query: 452 WQPFLNLLNRQDEFVQHMTARIIAKLACWHPQL 550
           W+PF  +L    E   H     IA+  C HP +
Sbjct: 142 WKPFNPILGETYEMTNHNGINFIAEQVCHHPPM 174


>At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein
           contains Pfam profile: PF01068 ATP dependent DNA ligase
           domain
          Length = 1417

 Score = 28.3 bits (60), Expect = 4.8
 Identities = 13/53 (24%), Positives = 23/53 (43%)
 Frame = -2

Query: 581 RDRSANHFCPSAADASKLAWQ*CEQSCAGQIHPDGSANSGRAAKRCQRISSHE 423
           R+   +H       +  + +  C ++C+ Q     S NSG A   C + S+ E
Sbjct: 685 REIPGDHAGERGLSSKTIQYPKCSEACSSQEDKKASENSGHAVNICVQTSAEE 737


>At4g31570.1 68417.m04483 expressed protein
          Length = 2712

 Score = 27.5 bits (58), Expect = 8.4
 Identities = 14/44 (31%), Positives = 22/44 (50%), Gaps = 3/44 (6%)
 Frame = +2

Query: 428  ETKFSGNVWQPFLNLLNRQDEFV---QHMTARIIAKLACWHPQL 550
            ET   GN+ +   +L    D+FV    H+  ++   LA WH Q+
Sbjct: 2135 ETSSGGNIAEICGSLSQNLDQFVVGVSHLEEKVSKHLATWHDQI 2178


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,897,704
Number of Sequences: 28952
Number of extensions: 265603
Number of successful extensions: 744
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)