Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10i16f
(646 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 250 2e-67
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 248 5e-67
SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 29 0.57
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 28 1.0
SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces pombe... 27 2.3
SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5 |Schizo... 26 4.0
SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 26 4.0
SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 26 5.3
SPCC162.01c |||RNA-binding protein |Schizosaccharomyces pombe|ch... 26 5.3
SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizos... 26 5.3
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 25 7.1
SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos... 25 9.3
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 25 9.3
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 250 bits (611), Expect = 2e-67
Identities = 123/177 (69%), Positives = 148/177 (83%)
Frame = +3
Query: 114 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 293
+ RE++VY AKLAEQAERY+EMVE MK VA N ++L+VEERNLLSVAYKN+IGARRA
Sbjct: 3 NSRENSVYLAKLAEQAERYEEMVENMKKVACSN---DKLSVEERNLLSVAYKNIIGARRA 59
Query: 294 SWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESK 473
SWRIISSIEQKEE++G + +I+ YR ++E EL DIC D+L VL+K+LIP++ TGESK
Sbjct: 60 SWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESK 119
Query: 474 VFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLAL 644
VFYYKMKGDY+RYLAEF G KEAA++SL AYKAASDIA+ ELPPT P+RLGLAL
Sbjct: 120 VFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLAL 176
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 248 bits (607), Expect = 5e-67
Identities = 123/177 (69%), Positives = 146/177 (82%)
Frame = +3
Query: 114 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 293
+ RED VY AKLAEQAERY+ MVE MK+VAS +D ELTVEERNLLSVAYKNVIGARRA
Sbjct: 4 TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVIGARRA 60
Query: 294 SWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESK 473
SWRI+SSIEQKEE+KG ++ +I+ YR ++E+EL IC DIL VL+K+LIP++ + ESK
Sbjct: 61 SWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESK 120
Query: 474 VFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLAL 644
VFYYKMKGDY+RYLAEFA G R+ +A+ SL YKAAS+IA EL PTHPIRLGLAL
Sbjct: 121 VFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLAL 177
>SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID
complex subunit Taf111|Schizosaccharomyces pombe|chr
1|||Manual
Length = 979
Score = 29.1 bits (62), Expect = 0.57
Identities = 21/85 (24%), Positives = 38/85 (44%)
Frame = +3
Query: 129 NVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRII 308
N + +A+Q Y+E + + N R +S N NL +A K I + +
Sbjct: 779 NAHAYNVAKQQRAYEEEINRIWNAQKRGLSIN-------NLEELAKKYGINSIHDDYVES 831
Query: 309 SSIEQKEETKGAEDKLNMIRAYRSQ 383
+ +EET ++ L ++R YR +
Sbjct: 832 NEETTREETPSSDKVLRIVRLYRDK 856
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 28.3 bits (60), Expect = 1.0
Identities = 23/100 (23%), Positives = 46/100 (46%)
Frame = +3
Query: 102 SSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIG 281
SST S N ++ Q+E Y ++ + + + ++SD E +E R V +N +
Sbjct: 45 SSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERRISEEVVRENSV- 103
Query: 282 ARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELR 401
+S +SS ET+ + + L + S +K+++
Sbjct: 104 ---SSIENVSSSAVARETQSSANILVKDNHFFSHKQKKIQ 140
>SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 520
Score = 27.1 bits (57), Expect = 2.3
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 469 DSPVCELGMRYLSRTPRMSEQMSRSSFSTW 380
DSP +G R +S+ R + RSS S+W
Sbjct: 481 DSPTKVIGKRNVSKASRTGQGRKRSSRSSW 510
>SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1065
Score = 26.2 bits (55), Expect = 4.0
Identities = 9/27 (33%), Positives = 19/27 (70%)
Frame = +3
Query: 357 NMIRAYRSQVEKELRDICSDILGVLDK 437
N +RA + +++K+L+D+ + +LDK
Sbjct: 243 NQLRADKKKLKKDLKDLVEEFQPILDK 269
>SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 599
Score = 26.2 bits (55), Expect = 4.0
Identities = 10/37 (27%), Positives = 22/37 (59%)
Frame = -1
Query: 424 PRMSEQMSRSSFSTWLRYALIMFNLSSAPFVSSFCSM 314
P ++E + +S S+W+ A ++ + + P V FC++
Sbjct: 121 PTIAEDFNDTSSSSWIGSAYVLASNAVLPAVGVFCNI 157
>SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 5.3
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = -2
Query: 231 SAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYT--HYLPFPTL 109
S+H + + H P F S QLTLL + YLP P +
Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528
>SPCC162.01c |||RNA-binding protein |Schizosaccharomyces pombe|chr
3|||Manual
Length = 244
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +2
Query: 266 QERDRRPPRLLADHLLHRTEGRNKRRRRQIEHDQS 370
Q RD P R + HR R + RR +EH+++
Sbjct: 53 QRRDYSPARRRDRYERHRESVREESPRRPVEHERN 87
>SPBC18H10.03 |tif35||translation initiation factor
eIF3g|Schizosaccharomyces pombe|chr 2|||Manual
Length = 282
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +3
Query: 387 EKELRDICSDILGVLDKYLIPSSQTGESKVFYY 485
E+ELRD+ G+ YL +TG +K F +
Sbjct: 215 EEELRDLFRRFGGIQRVYLAKDKETGRAKGFAF 247
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 25.4 bits (53), Expect = 7.1
Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Frame = +3
Query: 132 VYKAKLAEQAE----RYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARR 290
+YK KL E R DE+V MKN+ +N + +EL + ++N + + R
Sbjct: 202 IYKEKLRFSLESRKSRVDEIVNEMKNMCGKNPT-SELPTNYSDKTLTLWENELSSMR 257
>SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 584
Score = 25.0 bits (52), Expect = 9.3
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -2
Query: 135 THYLPFPTLWMTNDFTLTKS 76
T+YL FP + ND+TL+ +
Sbjct: 455 TYYLEFPGRFSVNDYTLSSN 474
>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
1|||Manual
Length = 809
Score = 25.0 bits (52), Expect = 9.3
Identities = 9/31 (29%), Positives = 18/31 (58%)
Frame = +3
Query: 123 EDNVYKAKLAEQAERYDEMVEAMKNVASRNV 215
ED++++ + +Q Y E +E K ++NV
Sbjct: 713 EDSMHEIAIKKQHSTYSEALEVAKKAGTKNV 743
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,268,967
Number of Sequences: 5004
Number of extensions: 43004
Number of successful extensions: 184
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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