BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10i02r (721 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 3.6 >SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 2344 Score = 26.6 bits (56), Expect = 3.6 Identities = 9/14 (64%), Positives = 12/14 (85%) Frame = +2 Query: 269 ILHLCLIYICLKNK 310 I+H CLI +CLKN+ Sbjct: 787 IMHACLIDVCLKNR 800 Score = 25.8 bits (54), Expect = 6.2 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 216 YKQHE*HHKCSFSVAVCLYYTFALFIF 296 Y QHE +K S +++ L YTF L F Sbjct: 2 YSQHELRNKVSLALSSLLRYTFELTPF 28 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,597,691 Number of Sequences: 5004 Number of extensions: 49804 Number of successful extensions: 90 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 86 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 90 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 337208592 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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