BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10h23r (707 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 140 2e-34 SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 139 4e-34 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 28 1.1 SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 28 1.1 SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 25 8.0 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 8.0 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 140 bits (338), Expect = 2e-34 Identities = 73/152 (48%), Positives = 99/152 (65%), Gaps = 2/152 (1%) Frame = -1 Query: 662 KGPXIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSAFGRLDPLF 483 +GP ++FN+D G+ +AFRNIPGVE LAPGGHLGRFVIWT+SAFG LD +F Sbjct: 205 RGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVF 264 Query: 482 GSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNN 309 GS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV RA +K NPL N Sbjct: 265 GSTTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNK 324 Query: 308 KAMLKLNPYAAVLKRKAILELRRRKNLKALAD 213 + +LNPYA KA ++L K KA + Sbjct: 325 AVLARLNPYAKAY--KANVKLNTGKTPKAAGE 354 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 139 bits (336), Expect = 4e-34 Identities = 72/152 (47%), Positives = 99/152 (65%), Gaps = 2/152 (1%) Frame = -1 Query: 662 KGPXIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSAFGRLDPLF 483 +GP ++FN+D G+ +AFRNIPGVE LAPGGHLGRFVIWT+SAFG LD +F Sbjct: 205 RGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVF 264 Query: 482 GSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNN 309 GS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV RA +K NPL N Sbjct: 265 GSTTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNK 324 Query: 308 KAMLKLNPYAAVLKRKAILELRRRKNLKALAD 213 + +LNPYA KA +++ K KA + Sbjct: 325 AVLSRLNPYAKAY--KANVKINSEKTPKAAGE 354 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 28.3 bits (60), Expect = 1.1 Identities = 27/109 (24%), Positives = 46/109 (42%) Frame = -1 Query: 461 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 282 KQK + + Q K LT+ S E + VL +R T+K LTN+ ++K +P Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPT 787 Query: 281 AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRERRRK 135 + ++ + + L ++ L+KRN K + RK Sbjct: 788 PQITVKQNTTKSSSAPRMGMLEQLKQE--YLTKRNFESKLKSSAVSSRK 834 >SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 28.3 bits (60), Expect = 1.1 Identities = 19/90 (21%), Positives = 43/90 (47%), Gaps = 2/90 (2%) Frame = -1 Query: 395 KSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKA-- 222 +S + + +K+ IR R+L+P ++ + N Y+ + + ++ R R Sbjct: 659 RSRSVTPINNINHKKYIRKDRELSPRGRERSSNR-NSYSDLSRSSSLSRGRSRSYTPEGR 717 Query: 221 LADAEKSGLKLSKRNPAMKAEKLRERRRKN 132 L ++E G + +PA + + R+R R++ Sbjct: 718 LIESEDKGYRSRSSSPASRKYRSRQRYRRS 747 >SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 25.4 bits (53), Expect = 8.0 Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 1/93 (1%) Frame = -1 Query: 413 DLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAM-LKLNPYAAVLKRKAILELRRR 237 ++T LK DE+RK+ N + L + +++ + L+ + K + +EL Sbjct: 366 EVTETLKGDELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMEL--- 422 Query: 236 KNLKALADAEKSGLKLSKRNPAMKAEKLRERRR 138 N LA+ E LKL + + R+RRR Sbjct: 423 -NKDYLAEEEAKNLKLQEDLKKGIVRQPRKRRR 454 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 25.4 bits (53), Expect = 8.0 Identities = 17/54 (31%), Positives = 32/54 (59%) Frame = +3 Query: 279 RVRIEFQHRLVVSERVQFACSTDHAFVGSTEDLPDLIRLEKTCEVSVGHLRLGQ 440 +V +EF+ RL + +RV+ AF+GS E + L+ + +T + ++ L LG+ Sbjct: 207 QVAVEFRKRLNIGDRVKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLA-LLLGR 258 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,267,269 Number of Sequences: 5004 Number of extensions: 40507 Number of successful extensions: 141 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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