Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10h23r
(707 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 142 2e-34
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 137 6e-33
At1g53510.1 68414.m06068 mitogen-activated protein kinase, putat... 35 0.046
At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 31 0.75
At3g10180.1 68416.m01219 kinesin motor protein-related similar t... 28 5.3
At3g58160.1 68416.m06485 myosin heavy chain, putative similar to... 28 7.0
At2g46980.2 68415.m05869 expressed protein 28 7.0
At2g46980.1 68415.m05868 expressed protein 28 7.0
At1g30950.1 68414.m03790 unusual floral organ (UFO) / F-box fami... 28 7.0
At3g47740.1 68416.m05201 ABC transporter family protein ATP bind... 27 9.2
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 142 bits (345), Expect = 2e-34
Identities = 74/168 (44%), Positives = 102/168 (60%), Gaps = 1/168 (0%)
Frame = -1
Query: 662 KGPXIIFNKDQG-LTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSAFGRLDPL 486
KGP +++ + + +AFRN+PGVE LAPGGHLGRFVIWT+SAF +L+ +
Sbjct: 210 KGPLVVYGTEGSKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESI 269
Query: 485 FGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNK 306
+GS++ PS++KK + LP+ KM N DL R++ SDEI+ V+ K RA K NPL N
Sbjct: 270 YGSFEKPSEKKKGYVLPRAKMVNADLARIINSDEIQSVVNPIKKDAKRAVLKKNPLKNLN 329
Query: 305 AMLKLNPYAAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKA 162
MLKLNPYA KR ++L +R K A+K + A+KA
Sbjct: 330 VMLKLNPYAKTAKRMSLLAEAQRVKAKKEKLAKKRKTVTKEEALAIKA 377
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 137 bits (332), Expect = 6e-33
Identities = 71/168 (42%), Positives = 99/168 (58%), Gaps = 1/168 (0%)
Frame = -1
Query: 662 KGPXIIFNKDQG-LTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSAFGRLDPL 486
KGP ++F + + +AFRN+PGVE LAPGGHLGRFVIWT+SAF +L+ +
Sbjct: 211 KGPLVVFGTEGAKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESI 270
Query: 485 FGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNK 306
+GS++ PS++KK + LP+ KM N DL R++ SDE++ V+ RA K NPL N
Sbjct: 271 YGSFEKPSEKKKGYVLPRAKMVNADLARIINSDEVQSVVNPIKDGSKRAVLKKNPLKNLN 330
Query: 305 AMLKLNPYAAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKA 162
M KLNPYA KR ++L R K +K + + A+KA
Sbjct: 331 VMFKLNPYAKTAKRMSLLAEASRVKAKKEKLEKKRKVVTKEEAQAIKA 378
>At1g53510.1 68414.m06068 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK18) mitogen-activated protein
kinase (MAPK)(AtMPK18), PMID:12119167
Length = 603
Score = 35.1 bits (77), Expect = 0.046
Identities = 32/99 (32%), Positives = 47/99 (47%), Gaps = 6/99 (6%)
Frame = -1
Query: 416 TDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYA-AVLKRKAILELRR 240
TDL KS+ I V ++ + RK NP+T ++ K +P A +L+R + +
Sbjct: 232 TDLLGTPKSETISGVRNDKARKYLTEMRKKNPVTFSQKFSKADPLALRLLQRLLAFDPKD 291
Query: 239 RKN-LKALADAEKSGLKLSKRNPA----MKAEKLRERRR 138
R +ALAD GL +R P+ K E ERRR
Sbjct: 292 RPTPAEALADPYFKGLSKIEREPSSQQISKMEFEFERRR 330
>At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N
domain-containing protein weak similarity to SP|P45974
Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)
{Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N
domain
Length = 413
Score = 31.1 bits (67), Expect = 0.75
Identities = 33/110 (30%), Positives = 49/110 (44%), Gaps = 1/110 (0%)
Frame = -1
Query: 461 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 282
K N N+ K A T LK+ E+R+ RA K+ K K +++
Sbjct: 162 KVPSNSNVGPAKPALTPEEVKLKAQELRE--RARKKK--EEEEKRMEREREKERIRIGK- 216
Query: 281 AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLR-ERRRK 135
+L+ K + E+ RK L L AEK K ++ K E+ + ERRRK
Sbjct: 217 -ELLEAKRMEEVNERKRLMFLRKAEKEEEKRAREKIRQKLEEDKAERRRK 265
>At3g10180.1 68416.m01219 kinesin motor protein-related similar to
centromere protein E GB:4502781 [Homo sapiens]
Length = 1348
Score = 28.3 bits (60), Expect = 5.3
Identities = 28/110 (25%), Positives = 49/110 (44%), Gaps = 2/110 (1%)
Frame = -1
Query: 467 PSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLN 288
P + K + QP + T ++ + + + K ++ + +TN + ++
Sbjct: 278 PYRDSKLTRILQPALGGNANTAIICNITLAPIHADETKSSLQFASRALRVTNCAHVNEIL 337
Query: 287 PYAAVLKR--KAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRER 144
AA+LKR K I ELR + +E+ L L RN +K+E RER
Sbjct: 338 TDAALLKRQKKEIEELRSKLKTSHSDHSEEEILNL--RNTLLKSELERER 385
>At3g58160.1 68416.m06485 myosin heavy chain, putative similar to
myosin heavy chain [Arabidopsis thaliana]
gi|602328|emb|CAA84067.
Length = 1242
Score = 27.9 bits (59), Expect = 7.0
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = -1
Query: 230 LKALADAEKSGLKLSKRNPAMKAEKLRERRRKNI 129
LKAL EK +S+R A +A +L ERRRK +
Sbjct: 1092 LKALLQVEKQRADISERKCA-EARELGERRRKRL 1124
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 27.9 bits (59), Expect = 7.0
Identities = 29/119 (24%), Positives = 53/119 (44%), Gaps = 4/119 (3%)
Frame = -1
Query: 473 KTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLK 294
+TP +K NF L Q K +N D L+K + ++ ATR+ P+T + +
Sbjct: 218 ETPEVEKTNFKLSQDKGSNDD--PLIKPRHNSDSIETDSESPENATRR--PVTRSLLQRR 273
Query: 293 LNPYAAVLKRKAILELRRR--KNLKALADAEKS--GLKLSKRNPAMKAEKLRERRRKNI 129
+ K KA L R+ + + ++ E+ G + N ++ +K R RR+ +
Sbjct: 274 VGAKGVQKKTKAGANLGRKCTEQVNSVFSFEEGLRGKIGTAVNSSVMPKKQRGRRKNTV 332
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 27.9 bits (59), Expect = 7.0
Identities = 29/119 (24%), Positives = 53/119 (44%), Gaps = 4/119 (3%)
Frame = -1
Query: 473 KTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLK 294
+TP +K NF L Q K +N D L+K + ++ ATR+ P+T + +
Sbjct: 218 ETPEVEKTNFKLSQDKGSNDD--PLIKPRHNSDSIETDSESPENATRR--PVTRSLLQRR 273
Query: 293 LNPYAAVLKRKAILELRRR--KNLKALADAEKS--GLKLSKRNPAMKAEKLRERRRKNI 129
+ K KA L R+ + + ++ E+ G + N ++ +K R RR+ +
Sbjct: 274 VGAKGVQKKTKAGANLGRKCTEQVNSVFSFEEGLRGKIGTAVNSSVMPKKQRGRRKNTV 332
>At1g30950.1 68414.m03790 unusual floral organ (UFO) / F-box family
protein (FBX1) E3 ubiquitin ligase SCF complex F-box
subunit; almost identical to unusual floral organs
(UFO)GI:4376159 from [Arabidopsis thaliana]
Landsberg-erecta; one amino acid difference
Length = 442
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 29 RPFQLSASYLPSSWEEAGSS*QPASWVS 112
R ++LS +Y+PS + +GSS SWVS
Sbjct: 138 RWYRLSFAYIPSGFYPSGSSGGLVSWVS 165
>At3g47740.1 68416.m05201 ABC transporter family protein ATP binding
cassette transporter ABC1, Homo sapiens, PIR2:A54774
Length = 947
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/39 (41%), Positives = 18/39 (46%)
Frame = +1
Query: 214 SARAFKFFLLRSSKIAFLFSTAA*GLSFSIALLLVSGFN 330
S KFFLL + F F L SIA LL S F+
Sbjct: 423 SVIGLKFFLLNDFSLQFSFYFVYINLQISIAFLLSSAFS 461
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,349,447
Number of Sequences: 28952
Number of extensions: 233788
Number of successful extensions: 677
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 664
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 676
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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