BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10h10f
(616 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g69730.1 68414.m08024 protein kinase family protein contains ... 32 0.35
At1g54450.1 68414.m06211 calcium-binding EF-hand family protein ... 30 1.4
At1g69790.1 68414.m08030 protein kinase, putative similar to pro... 29 3.2
At5g63540.1 68418.m07975 expressed protein ; expression support... 28 5.7
At4g14310.2 68417.m02205 peroxisomal membrane protein-related co... 27 7.5
At4g14310.1 68417.m02204 peroxisomal membrane protein-related co... 27 7.5
At3g19380.1 68416.m02458 U-box domain-containing protein contain... 27 7.5
At2g30250.1 68415.m03682 WRKY family transcription factor 27 7.5
At1g58060.1 68414.m06580 helicase domain-containing protein cont... 27 7.5
At4g23990.1 68417.m03448 cellulose synthase family protein simil... 27 9.9
>At1g69730.1 68414.m08024 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 792
Score = 31.9 bits (69), Expect = 0.35
Identities = 18/58 (31%), Positives = 27/58 (46%)
Frame = +3
Query: 264 PVYIHRGNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNN 437
P Y+ + N L G L + ++ CT S T H ++ LAV ++CQN N
Sbjct: 128 PFYVSQHNELVAVGCNNTASLTNVKPSIVQCTSSCSTKPHTHIKDYLAV--LNCQNYN 183
>At1g54450.1 68414.m06211 calcium-binding EF-hand family protein
contains Pfam profile: PF00036 EF hand
Length = 535
Score = 29.9 bits (64), Expect = 1.4
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +3
Query: 96 LPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDFRSGCTFRV 239
LPE ++ N+D + D +LAL ++ L ND ++G V
Sbjct: 13 LPETSPMSMKSNQDFVKKLFDQWLALPETNRLVTSLVNDAKAGVALNV 60
>At1g69790.1 68414.m08030 protein kinase, putative similar to
protein kinase APK1A [Arabidopsis thaliana]
SWISS-PROT:Q06548
Length = 387
Score = 28.7 bits (61), Expect = 3.2
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +3
Query: 360 GSGRTIRHPNVASNLAVGTV-SCQNNNLVTANW 455
GS R R PN +S L+ T+ S NN+ T++W
Sbjct: 20 GSSRISRKPNQSSRLSSLTIPSYSNNSFTTSSW 52
>At5g63540.1 68418.m07975 expressed protein ; expression supported
by MPSS
Length = 602
Score = 27.9 bits (59), Expect = 5.7
Identities = 23/92 (25%), Positives = 42/92 (45%), Gaps = 5/92 (5%)
Frame = +3
Query: 270 YIH-RGNYL--SPTGNTGQIRLNRGEQVLIACT-GSGRTIRHPNVASNLAVGTVSCQNNN 437
++H GN++ S +GN + N+G V + T S R + P ++N+ ++
Sbjct: 270 HVHDTGNHVNSSASGNASHTQANQGIPVHVTRTHNSSRAMDEPLASTNVGATVSRVEHMQ 329
Query: 438 LVTANWLRGNSAFGQL-TCSSHAHHDAQQTNT 530
+ TA+ G F + + SS+ H A T
Sbjct: 330 IDTAS-AHGERTFSDIHSTSSNIHRAASTAGT 360
>At4g14310.2 68417.m02205 peroxisomal membrane protein-related
contains weak similarity to Peroxisomal membrane protein
2 (22 kDa peroxisomal membrane protein)
(Swiss-Prot:P42925) [Mus musculus]
Length = 965
Score = 27.5 bits (58), Expect = 7.5
Identities = 21/78 (26%), Positives = 32/78 (41%)
Frame = +3
Query: 282 GNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLR 461
G + G Q +RG+ V + CT T++ +AS+ V S + LV
Sbjct: 816 GAKIPKLGVNAQCVSSRGDSVFLGCTNQKSTVK-KQMASSSQVQQFSIRKQRLV------ 868
Query: 462 GNSAFGQLTCSSHAHHDA 515
S + +SH HH A
Sbjct: 869 --STYSLPDSNSHPHHSA 884
>At4g14310.1 68417.m02204 peroxisomal membrane protein-related
contains weak similarity to Peroxisomal membrane protein
2 (22 kDa peroxisomal membrane protein)
(Swiss-Prot:P42925) [Mus musculus]
Length = 1087
Score = 27.5 bits (58), Expect = 7.5
Identities = 21/78 (26%), Positives = 32/78 (41%)
Frame = +3
Query: 282 GNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLR 461
G + G Q +RG+ V + CT T++ +AS+ V S + LV
Sbjct: 789 GAKIPKLGVNAQCVSSRGDSVFLGCTNQKSTVK-KQMASSSQVQQFSIRKQRLV------ 841
Query: 462 GNSAFGQLTCSSHAHHDA 515
S + +SH HH A
Sbjct: 842 --STYSLPDSNSHPHHSA 857
>At3g19380.1 68416.m02458 U-box domain-containing protein contains
similarity to immediate-early fungal elicitor protein
CMPG1 GI:14582200 [Petroselinum crispum]; contains Pfam
profile PF04564: U-box domain
Length = 421
Score = 27.5 bits (58), Expect = 7.5
Identities = 19/87 (21%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = +1
Query: 226 AHSESTVILVSLNLYISTEEII*VQLVIQVRFVLIVGNKFLSLAQDPA-VRSVTPMLLLT 402
AH+ + +++ L ++ E++ L + V + N+F+S++ DP V +T +L +
Sbjct: 148 AHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDS 207
Query: 403 LLSGRSAVKTIISSQPIGCVVTVLSGN 483
+ R +I G L G+
Sbjct: 208 SIETRVNAAALIEIVSTGTKSADLKGS 234
>At2g30250.1 68415.m03682 WRKY family transcription factor
Length = 393
Score = 27.5 bits (58), Expect = 7.5
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = +3
Query: 81 KLRTDLPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDF 215
K +T P P ++ + F DYLD+ L L S L + T F
Sbjct: 45 KFKTAQPPPLPISQSSHNFTFSDYLDSPLLLSSSHSLISPTTGTF 89
>At1g58060.1 68414.m06580 helicase domain-containing protein contains
similarity to SP|P24785 Dosage compensation regulator
(Male-less protein) (No action potential protein)
{Drosophila melanogaster}; contains Pfam profiles
PF04408: Helicase associated domain (HA2), PF00271:
Helicase conserved C-terminal domain
Length = 1459
Score = 27.5 bits (58), Expect = 7.5
Identities = 17/70 (24%), Positives = 31/70 (44%)
Frame = +3
Query: 381 HPNVASNLAVGTVSCQNNNLVTANWLRGNSAFGQLTCSSHAHHDAQQTNTRCFNNHFVIR 560
+PN+A+N T + N+ N + SA+ H H + +N + F N F++
Sbjct: 1299 YPNIAANDKGITETTFNSLTKQGNQTKSYSAWYDGRREVHIHPSSINSNFKAFQNPFLVF 1358
Query: 561 VGFIVNNVFY 590
+ + N Y
Sbjct: 1359 LEKVETNKVY 1368
>At4g23990.1 68417.m03448 cellulose synthase family protein similar
to cellulose synthase catalytic subunit from Arabidopsis
thaliana [gi:5230423], cellulose synthase-5 from Zea
mays [gi:9622882]
Length = 732
Score = 27.1 bits (57), Expect = 9.9
Identities = 12/43 (27%), Positives = 25/43 (58%)
Frame = +1
Query: 460 VVTVLSGNSHALLTLTMTLNKPTLDASIITLLFVWDSSSTMYF 588
++ ++ + H+LLT TL L S I L F+W +++++ +
Sbjct: 37 IIALMYHHVHSLLTANTTLITSLLLLSDIVLAFMWATTTSLRY 79
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,098,046
Number of Sequences: 28952
Number of extensions: 269249
Number of successful extensions: 721
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 707
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 721
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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