Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10h04r
(714 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g23880.1 68418.m02805 cleavage and polyadenylation specificit... 93 2e-19
At4g11040.1 68417.m01795 protein phosphatase 2C, putative / PP2C... 31 0.76
At2g01730.1 68415.m00101 metallo-beta-lactamase family protein s... 30 1.3
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 29 2.3
At1g66150.1 68414.m07508 leucine-rich repeat protein kinase, put... 29 4.0
At5g25520.2 68418.m03037 transcription elongation factor-related... 28 5.3
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 28 7.1
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 28 7.1
At2g43110.1 68415.m05352 expressed protein 28 7.1
>At5g23880.1 68418.m02805 cleavage and polyadenylation specificity
factor identical to cleavage and polyadenylation
specificity factor [Arabidopsis thaliana]
SWISS-PROT:Q9LKF9
Length = 739
Score = 93.1 bits (221), Expect = 2e-19
Identities = 62/227 (27%), Positives = 108/227 (47%), Gaps = 10/227 (4%)
Frame = -1
Query: 690 PSKCVSSVRQVHVKAHVEYIELEGRCDGESLLRVVSQTKPWAVVGLRASEAALHTLKKHC 511
PSK +S+ V V + ++ EGR DG S+ +++ P +V + A A LK+HC
Sbjct: 523 PSKVMSNELIVTVSCSLVKMDYEGRSDGRSIKSMIAHVSPLKLVLVHAIAEATEHLKQHC 582
Query: 510 ESEGIEKVFLPSVGETVDATTESHIYQVKLTDGLMRGLVWRQAGDAELAWLSAVVAPRTH 331
+ V+ P + ETVD T++ Y+V+L++ LM +++++ GD+E+AW+ + V +T
Sbjct: 583 LNNICPHVYAPQIEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSEVG-KTE 641
Query: 330 ARESDAAPEEGGGAVSLEAGAGRPRAAAFVNTVXXXXXXXXXXXXXLQPDLGAGTLTCCS 151
P GA P V + +Q + G C
Sbjct: 642 RDMRSLLP---------MPGAASPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCG 692
Query: 150 GTLAVRRM----ENG------QVSLEGVLSEEYYKVRELLYEQFAIV 40
+ +R++ + G Q+ +EG L E+YYK+R+ LY QF ++
Sbjct: 693 EYVTLRKVGPTGQKGGASGPQQILIEGPLCEDYYKIRDYLYSQFYLL 739
>At4g11040.1 68417.m01795 protein phosphatase 2C, putative / PP2C,
putative phosphoprotein phosphatase (EC 3.1.3.16) 2C -
Arabidopsis thaliana, PIR2:S55457
Length = 295
Score = 31.1 bits (67), Expect = 0.76
Identities = 18/45 (40%), Positives = 21/45 (46%)
Frame = +1
Query: 457 VDGFPNGR*EHLFDSFRLTMFFEGVQRRLAGPEPHHCPGLGLRHD 591
VD P+ +FD RL FFE RRL E C G G+ D
Sbjct: 99 VDEIPSYDIFGIFDGLRLAKFFEDRLRRLVKEEVKACHGRGVAAD 143
>At2g01730.1 68415.m00101 metallo-beta-lactamase family protein
simliar to SP|P79101 Cleavage and polyadenylation
specificity factor, 73 kDa subunit (CPSF 73 kDa subunit)
{Bos taurus}; contains Pfam profile PF00753:
Metallo-beta-lactamase superfamily
Length = 613
Score = 30.3 bits (65), Expect = 1.3
Identities = 18/76 (23%), Positives = 35/76 (46%)
Frame = -1
Query: 663 QVHVKAHVEYIELEGRCDGESLLRVVSQTKPWAVVGLRASEAALHTLKKHCESEGIEKVF 484
+V V+ V + D + ++ + P VV + + ++ LK+ SE F
Sbjct: 377 KVDVRCKVHQVAFSPHTDAKGIMDLTKFLSPKNVVLVHGEKPSMMILKEKITSELDIPCF 436
Query: 483 LPSVGETVDATTESHI 436
+P+ GETV + ++I
Sbjct: 437 VPANGETVSFASTTYI 452
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 29.5 bits (63), Expect = 2.3
Identities = 23/95 (24%), Positives = 43/95 (45%)
Frame = -1
Query: 696 EVPSKCVSSVRQVHVKAHVEYIELEGRCDGESLLRVVSQTKPWAVVGLRASEAALHTLKK 517
+ P+K + + + A + E++ + DG+S S K VG ++S++ +K
Sbjct: 822 QTPAKTIVKKKIIKRVAKRKVAEIDNKMDGDSKKDGDSDEKKVMEVGKKSSDSGSVEMKP 881
Query: 516 HCESEGIEKVFLPSVGETVDATTESHIYQVKLTDG 412
ES +E V + +TVD E+ K +G
Sbjct: 882 TAES--LEDVKDENASKTVDVKQETGSPDTKKKEG 914
>At1g66150.1 68414.m07508 leucine-rich repeat protein kinase,
putative (TMK1) identical to protein kinase TMK1
gi|166888|gb|AAA32876, SP|P43298 Putative receptor
protein kinase TMK1 precursor (EC 2.7.1.-) {Arabidopsis
thaliana}
Length = 942
Score = 28.7 bits (61), Expect = 4.0
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -1
Query: 186 PDLGAGTLTCCSGTLAVRRMENGQVSLEGVLSEEYYKVREL 64
PD T C+GT V R++ G L+G LS + + EL
Sbjct: 50 PDPCKWTHIVCTGTKRVTRIQIGHSGLQGTLSPDLRNLSEL 90
>At5g25520.2 68418.m03037 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 997
Score = 28.3 bits (60), Expect = 5.3
Identities = 19/75 (25%), Positives = 42/75 (56%), Gaps = 6/75 (8%)
Frame = -1
Query: 588 VSQTKPWAVVGLRASEAALHTLKKHCESEGIEKVFLPSVGETVD----ATTESHIYQVKL 421
+SQ++ +++ + S A + + G+E P++GET+D ++ ++ +VK
Sbjct: 726 ISQSQRDSLIEVAKSYVADQRVGYAEPTSGVELYLCPTLGETLDLLSKIISKDYLDEVKC 785
Query: 420 TD--GLMRGLVWRQA 382
++ GL+ +VWR+A
Sbjct: 786 SEDIGLIGVVVWRRA 800
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 27.9 bits (59), Expect = 7.1
Identities = 33/142 (23%), Positives = 58/142 (40%), Gaps = 5/142 (3%)
Frame = -1
Query: 708 EEMSEVPSKCVSSVRQVHVKAHVEYIELEGRCDGE---SLLRVVSQTKPWAVVGLRASEA 538
EE++ + S+ S + H + +EL D E SL + + + + +
Sbjct: 422 EEINRLQSEFSSYKIRAHALLQKKDMELAAAKDSEQIKSLEEALKEAEKEVYLVSAERDR 481
Query: 537 ALHTLKKHCESEGIEKVFLPSVGETVDATTESHIYQVKLTDGLMRGLVWRQAGDAELAWL 358
A L+ S +EK G DA+ + +VKL + R +QA + +L L
Sbjct: 482 AQQDLQSALAS--LEKELEERAGALKDASEQIKSLEVKLDSTVARNQAEKQAWEEDLRVL 539
Query: 357 SAVVAPRTHA--RESDAAPEEG 298
R A +++A+P EG
Sbjct: 540 EETWRRRCEALTAQNEASPAEG 561
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 27.9 bits (59), Expect = 7.1
Identities = 33/142 (23%), Positives = 58/142 (40%), Gaps = 5/142 (3%)
Frame = -1
Query: 708 EEMSEVPSKCVSSVRQVHVKAHVEYIELEGRCDGE---SLLRVVSQTKPWAVVGLRASEA 538
EE++ + S+ S + H + +EL D E SL + + + + +
Sbjct: 422 EEINRLQSEFSSYKIRAHALLQKKDMELAAAKDSEQIKSLEEALKEAEKEVYLVSAERDR 481
Query: 537 ALHTLKKHCESEGIEKVFLPSVGETVDATTESHIYQVKLTDGLMRGLVWRQAGDAELAWL 358
A L+ S +EK G DA+ + +VKL + R +QA + +L L
Sbjct: 482 AQQDLQSALAS--LEKELEERAGALKDASEQIKSLEVKLDSTVARNQAEKQAWEEDLRVL 539
Query: 357 SAVVAPRTHA--RESDAAPEEG 298
R A +++A+P EG
Sbjct: 540 EETWRRRCEALTAQNEASPAEG 561
>At2g43110.1 68415.m05352 expressed protein
Length = 288
Score = 27.9 bits (59), Expect = 7.1
Identities = 24/71 (33%), Positives = 35/71 (49%), Gaps = 6/71 (8%)
Frame = -1
Query: 705 EMSEVPSKCVSSVRQVHVKAHVEYIELEGRCDGESLLRVVSQTKPWAVV----GLRASE- 541
E+S+ + VS++ + H+K E C+GESL R V P +V LR+ E
Sbjct: 110 ELSQGLDQDVSNLGE-HIKLSCGSSWRETLCEGESLERKVEPGNPSVLVISSSALRSLEL 168
Query: 540 -AALHTLKKHC 511
LH+L K C
Sbjct: 169 LRGLHSLTKQC 179
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,876,914
Number of Sequences: 28952
Number of extensions: 186003
Number of successful extensions: 552
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 543
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 552
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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