Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10h04f
         (635 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g23880.1 68418.m02805 cleavage and polyadenylation specificit...   173   6e-44
At2g01730.1 68415.m00101 metallo-beta-lactamase family protein s...    55   5e-08
At1g61010.2 68414.m06870 cleavage and polyadenylation specificit...    49   2e-06
At1g61010.1 68414.m06869 cleavage and polyadenylation specificit...    49   2e-06
At5g63420.1 68418.m07962 metallo-beta-lactamase family protein         36   0.017
At3g07530.1 68416.m00899 expressed protein ; expression supporte...    36   0.017
At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative s...    31   0.85 
At5g64050.1 68418.m08042 glutamate-tRNA ligase family protein / ...    29   2.6  
At5g60610.1 68418.m07606 F-box family protein contains F-box dom...    28   4.5  
At2g21890.1 68415.m02601 mannitol dehydrogenase, putative simila...    28   4.5  
At1g74700.1 68414.m08652 RNase Z identical to RNase Z (GI:209756...    28   6.0  
At1g58200.2 68414.m06607 mechanosensitive ion channel domain-con...    28   6.0  
At1g58200.1 68414.m06606 mechanosensitive ion channel domain-con...    28   6.0  
At3g13150.1 68416.m01645 pentatricopeptide (PPR) repeat-containi...    27   7.9  

>At5g23880.1 68418.m02805 cleavage and polyadenylation specificity
           factor identical to cleavage and polyadenylation
           specificity factor [Arabidopsis thaliana]
           SWISS-PROT:Q9LKF9
          Length = 739

 Score =  173 bits (422), Expect = 6e-44
 Identities = 77/156 (49%), Positives = 110/156 (70%)
 Frame = +3

Query: 168 MTSIIKLHCLSGAGEETPPCYVLQIDEFKFLLDCGWDEKFDMVFIKELKRHVNSIDAVLI 347
           M + +++  L G   E P  Y++ ID F FL+DCGW++ FD   ++ L R  ++IDAVL+
Sbjct: 1   MGTSVQVTPLCGVYNENPLSYLVSIDGFNFLIDCGWNDLFDTSLLEPLSRVASTIDAVLL 60

Query: 348 SHSDPLHLGALPYAVGKLGLSCPIYATLPVYKMGQMFMYDLYQAHKNVSEFDLFTLDDVD 527
           SH D LH+GALPYA+ +LGLS P+YAT PV+++G + MYD + + K VS+FDLFTLDD+D
Sbjct: 61  SHPDTLHIGALPYAMKQLGLSAPVYATEPVHRLGLLTMYDQFLSRKQVSDFDLFTLDDID 120

Query: 528 MAFDRIVQLKYNQSIEMKGKGLGLRITPLPAGHSLG 635
            AF  +++L Y+Q+  + GKG G+ I P  AGH LG
Sbjct: 121 SAFQNVIRLTYSQNYHLSGKGEGIVIAPHVAGHMLG 156


>At2g01730.1 68415.m00101 metallo-beta-lactamase family protein
           simliar to SP|P79101 Cleavage and polyadenylation
           specificity factor, 73 kDa subunit (CPSF 73 kDa subunit)
           {Bos taurus}; contains Pfam profile PF00753:
           Metallo-beta-lactamase superfamily
          Length = 613

 Score = 54.8 bits (126), Expect = 5e-08
 Identities = 44/160 (27%), Positives = 71/160 (44%), Gaps = 10/160 (6%)
 Frame = +3

Query: 186 LHCLS-GAGEET-PPCYVLQIDEFKFLLDCGWDEKFD-------MVFIKELKRHVNSIDA 338
           + CL  GAG+E    C V+ I+  K + DCG     D          I +     N+I  
Sbjct: 3   IDCLVLGAGQEIGKSCVVVTINGKKIMFDCGMHMGCDDHNRYPNFSLISKSGDFDNAISC 62

Query: 339 VLISHSDPLHLGALPYAVGKLGLSCPIYATLPVYKMGQMFMYDLYQAH-KNVSEFDLFTL 515
           ++I+H    H+GALPY     G + PIY + P   +  + + D  +       E +LFT 
Sbjct: 63  IIITHFHMDHVGALPYFTEVCGYNGPIYMSYPTKALSPLMLEDYRRVMVDRRGEEELFTT 122

Query: 516 DDVDMAFDRIVQLKYNQSIEMKGKGLGLRITPLPAGHSLG 635
             +     +++ +   Q+I++      L+I    AGH LG
Sbjct: 123 THIANCMKKVIAIDLKQTIQVDE---DLQIRAYYAGHVLG 159


>At1g61010.2 68414.m06870 cleavage and polyadenylation specificity
           factor, putative similar to cleavage and polyadenylation
           specificity factor 73 kDa subunit [Homo sapiens]
           SWISS-PROT:Q9UKF6
          Length = 693

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 37/148 (25%), Positives = 66/148 (44%), Gaps = 3/148 (2%)
 Frame = +3

Query: 201 GAGEETP-PCYVLQIDEFKFLLDCGWDEKFDMVFIKELKRHVN--SIDAVLISHSDPLHL 371
           GAG E    C  +       L DCG    +  +        ++  SID +LI+H    H 
Sbjct: 28  GAGSEVGRSCVYMSFRGKNILFDCGIHPAYSGMAALPYFDEIDPSSIDVLLITHFHIDHA 87

Query: 372 GALPYAVGKLGLSCPIYATLPVYKMGQMFMYDLYQAHKNVSEFDLFTLDDVDMAFDRIVQ 551
            +LPY + K   +  ++ T     + ++ + D  +  K   E  LF   D++ + D+I  
Sbjct: 88  ASLPYFLEKTTFNGRVFMTHATKAIYKLLLTDYVKVSKVSVEDMLFDEQDINKSMDKIEV 147

Query: 552 LKYNQSIEMKGKGLGLRITPLPAGHSLG 635
           + ++Q++E+     G++     AGH LG
Sbjct: 148 IDFHQTVEVN----GIKFWCYTAGHVLG 171


>At1g61010.1 68414.m06869 cleavage and polyadenylation specificity
           factor, putative similar to cleavage and polyadenylation
           specificity factor 73 kDa subunit [Homo sapiens]
           SWISS-PROT:Q9UKF6
          Length = 693

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 37/148 (25%), Positives = 66/148 (44%), Gaps = 3/148 (2%)
 Frame = +3

Query: 201 GAGEETP-PCYVLQIDEFKFLLDCGWDEKFDMVFIKELKRHVN--SIDAVLISHSDPLHL 371
           GAG E    C  +       L DCG    +  +        ++  SID +LI+H    H 
Sbjct: 28  GAGSEVGRSCVYMSFRGKNILFDCGIHPAYSGMAALPYFDEIDPSSIDVLLITHFHIDHA 87

Query: 372 GALPYAVGKLGLSCPIYATLPVYKMGQMFMYDLYQAHKNVSEFDLFTLDDVDMAFDRIVQ 551
            +LPY + K   +  ++ T     + ++ + D  +  K   E  LF   D++ + D+I  
Sbjct: 88  ASLPYFLEKTTFNGRVFMTHATKAIYKLLLTDYVKVSKVSVEDMLFDEQDINKSMDKIEV 147

Query: 552 LKYNQSIEMKGKGLGLRITPLPAGHSLG 635
           + ++Q++E+     G++     AGH LG
Sbjct: 148 IDFHQTVEVN----GIKFWCYTAGHVLG 171


>At5g63420.1 68418.m07962 metallo-beta-lactamase family protein 
          Length = 911

 Score = 36.3 bits (80), Expect = 0.017
 Identities = 15/40 (37%), Positives = 27/40 (67%)
 Frame = +3

Query: 309 LKRHVNSIDAVLISHSDPLHLGALPYAVGKLGLSCPIYAT 428
           ++R  + I+AV+I+H    H+GALP+ +  L  + PI+A+
Sbjct: 161 IRRWKHKIEAVVITHGHEDHIGALPWVIPALDPNTPIFAS 200


>At3g07530.1 68416.m00899 expressed protein ; expression supported
           by MPSS
          Length = 699

 Score = 36.3 bits (80), Expect = 0.017
 Identities = 21/52 (40%), Positives = 27/52 (51%)
 Frame = +3

Query: 330 IDAVLISHSDPLHLGALPYAVGKLGLSCPIYATLPVYKMGQMFMYDLYQAHK 485
           ID VLIS+  P+ L  LP+     G    IY T    K+GQ+ M D+   HK
Sbjct: 103 IDIVLISN--PMGLLGLPFLTQNPGFFAKIYMTEVTAKIGQLMMEDIVSMHK 152



 Score = 33.5 bits (73), Expect = 0.12
 Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
 Frame = +3

Query: 180 IKLHCLS-GAGEETPPCYVLQIDEFKFLLDCGWD 278
           ++L CLS G G   PPC++L +  F+ L+DC  D
Sbjct: 1   MELTCLSKGDGFHYPPCHMLNLCGFRILIDCPLD 34


>At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative
           similar to Swiss-Prot:Q9NG98 DNA topoisomerase III alpha
           [Drosophila melanogaster]
          Length = 926

 Score = 30.7 bits (66), Expect = 0.85
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +1

Query: 400 SVLAVQYMQLYRYTKWGRCSCTIYTKPIRMCPSSI 504
           ++LA  Y+++YR+  WG     +Y K  +  P+++
Sbjct: 457 AILAKNYLEVYRFESWGGSVIPVYEKGQQFIPTTL 491


>At5g64050.1 68418.m08042 glutamate-tRNA ligase family protein /
           glutamyl-tRNA synthetase family protein similar to
           SP|P22250 from Bacillus subtilis, SP|P22249 from
           Bacillus stearothermophilus; contains Pfam tRNA
           synthetases class I (E and Q), catalytic domain PF00749
          Length = 570

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 17/37 (45%), Positives = 19/37 (51%)
 Frame = +2

Query: 371 RSSSICSWKTRS*LSNICNSTGIQNGADVHVRFIPSP 481
           R SS+     RS     C ST + NG  V VRF PSP
Sbjct: 27  RQSSLFYCSRRSFAVVAC-STPVNNGGSVRVRFAPSP 62


>At5g60610.1 68418.m07606 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 388

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 21/66 (31%), Positives = 31/66 (46%), Gaps = 4/66 (6%)
 Frame = +1

Query: 361 LYI*ELFHMQLEN--SVLAVQYMQLYRYTKWGRCSCTIYTKPIRMCPSSICS-HW-MMLI 528
           +Y+ +     LE+  SV  +   QLY      +C   I  K +     SICS +W  ++I
Sbjct: 248 IYVTQHIETLLESVTSVKRLTLRQLYNSIDEYKCRAGIVFKQLEQLELSICSDNWTKLVI 307

Query: 529 WLLTES 546
           WLL  S
Sbjct: 308 WLLQNS 313


>At2g21890.1 68415.m02601 mannitol dehydrogenase, putative similar
           to ELI3-2 (SP|Q02972), sinapyl alcohol dehydrogenase
           (Populus tremuloides) (gi:14279694); contains Pfam
           zinc-binding dehydrogenase domain PF00107
          Length = 375

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = +3

Query: 459 MYDLYQAHKNVSEFDLFTLDDVDMAFDRIVQ 551
           M +    HK VS+ +L  + D++ A DR+V+
Sbjct: 307 MLEFCAKHKIVSDIELIKMSDINSAMDRLVK 337


>At1g74700.1 68414.m08652 RNase Z identical to RNase Z (GI:20975609)
           [Arabidopsis thaliana]
          Length = 280

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 18/60 (30%), Positives = 28/60 (46%)
 Frame = +3

Query: 327 SIDAVLISHSDPLHLGALPYAVGKLGLSCPIYATLPVYKMGQMFMYDLYQAHKNVSEFDL 506
           S D + ISHS   H+G LP  V   GL      T+ V    +  +  L++ H+ +   +L
Sbjct: 46  SQDFLFISHSHMDHIGGLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSEL 105


>At1g58200.2 68414.m06607 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 678

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 21/73 (28%), Positives = 39/73 (53%), Gaps = 5/73 (6%)
 Frame = -2

Query: 445 ILYTGRVAYIGQLRPS-FPTAYGRAPKCRGSECDISTASI-EFTCLLSSLMKTISNF--- 281
           +L++G +     L P   P++ G+A K R      S +++  F+C LSSL++ +  F   
Sbjct: 165 LLWSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFME 224

Query: 280 SSHPQSRRNLNSS 242
           +++P   RN+  S
Sbjct: 225 TNNPADTRNMGFS 237


>At1g58200.1 68414.m06606 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 678

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 21/73 (28%), Positives = 39/73 (53%), Gaps = 5/73 (6%)
 Frame = -2

Query: 445 ILYTGRVAYIGQLRPS-FPTAYGRAPKCRGSECDISTASI-EFTCLLSSLMKTISNF--- 281
           +L++G +     L P   P++ G+A K R      S +++  F+C LSSL++ +  F   
Sbjct: 165 LLWSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFME 224

Query: 280 SSHPQSRRNLNSS 242
           +++P   RN+  S
Sbjct: 225 TNNPADTRNMGFS 237


>At3g13150.1 68416.m01645 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 551

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 15/45 (33%), Positives = 28/45 (62%)
 Frame = -2

Query: 340 TASIEFTCLLSSLMKTISNFSSHPQSRRNLNSSIWST*HGGVSSP 206
           ++S + +  +SS   + S+FSS P S  +++SS++S+     SSP
Sbjct: 465 SSSPDSSSSVSSSPDSYSSFSSSPDSSSSVSSSLFSSSRENSSSP 509


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,869,873
Number of Sequences: 28952
Number of extensions: 285879
Number of successful extensions: 620
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 620
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)