BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10g20r (666 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 82 6e-17 SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 66 4e-12 SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 26 5.6 SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 26 5.6 SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 25 7.4 SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha... 25 9.8 SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch... 25 9.8 SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 25 9.8 >SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E 4F complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 243 Score = 82.2 bits (194), Expect = 6e-17 Identities = 42/135 (31%), Positives = 66/135 (48%), Gaps = 3/135 (2%) Frame = -1 Query: 552 DGDTSLQSESSPAIKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMK 379 +G +++ +E++ HPL+H W+ W ++ EW L E+ +F TVE++W ++ + Sbjct: 49 EGLSAVNAETAFVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTIS 108 Query: 378 LPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXLMIGENF 199 S L DY F KGI+P WEDP N GG+W N IGE Sbjct: 109 KASMLPAKSDYSYFLKGIRPEWEDPQNMNGGKWAYQSKHKGSNLDELWLYMVLAAIGETL 168 Query: 198 EHPDL-ICGVVVNIK 157 + + GVV N++ Sbjct: 169 DPTGKEVTGVVCNMR 183 >SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 218 Score = 66.1 bits (154), Expect = 4e-12 Identities = 34/123 (27%), Positives = 58/123 (47%), Gaps = 4/123 (3%) Frame = -1 Query: 513 IKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 340 +KHPL W+ W + EW + TF +VE++W +++ + S L DY Sbjct: 36 LKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDYSF 95 Query: 339 FKKGIQPTWEDPINEKGGRWII-CESKSARNXXXXXXXXXXLMIGENFEHP-DLICGVVV 166 F++G++P WED N+ GG+W + + IGE + + GVV+ Sbjct: 96 FREGVRPEWEDVHNKTGGKWAFQNKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMGVVI 155 Query: 165 NIK 157 N++ Sbjct: 156 NMR 158 >SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1233 Score = 25.8 bits (54), Expect = 5.6 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +2 Query: 206 SPIISRIRSNHRGSRLRADLLS 271 SP+ + R H+G+RL D+L+ Sbjct: 586 SPVNQKFRGTHKGARLAIDVLN 607 >SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual Length = 935 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/22 (50%), Positives = 13/22 (59%) Frame = +3 Query: 543 YHHLASHVDWTVQTSLLIFFKI 608 + H V W+V TSLL FF I Sbjct: 597 FSHKTISVSWSVLTSLLCFFTI 618 >SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 25.4 bits (53), Expect = 7.4 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = -1 Query: 396 LYHYMKLPSELNHGQDYMIFKKGIQPTW 313 L +Y KLPSE N+G + + P W Sbjct: 394 LENYFKLPSEYNNGISFAAKGRRKTPLW 421 >SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 25.0 bits (52), Expect = 9.8 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = +3 Query: 357 HGLTLKVVSCSGINTSNLLQ*RMLIIQPSCVSILYSYLCKASSS 488 H LT+ V +C+ + N L+ S +L+SYLCK +S Sbjct: 220 HPLTV-VYTCAQVVLDNRTSIIELLWDNSGADLLHSYLCKLPAS 262 >SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr 2|||Manual Length = 333 Score = 25.0 bits (52), Expect = 9.8 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = -1 Query: 498 EHTWSYWLYTNKSKEWIH 445 +H W+ +Y+NK + W+H Sbjct: 189 DHVWTE-VYSNKQQRWVH 205 >SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1367 Score = 25.0 bits (52), Expect = 9.8 Identities = 11/41 (26%), Positives = 23/41 (56%) Frame = +2 Query: 74 SLLLFCRSSYFSVCLHHVSIMSHILLFALILTTTPQIKSGC 196 + +LF Y +++ ++++LF L+ T+ P I +GC Sbjct: 1104 TFILFWYQFYNEFDGNYIFDYTYVMLFNLLFTSLPVIIAGC 1144 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,716,664 Number of Sequences: 5004 Number of extensions: 56434 Number of successful extensions: 158 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 303841898 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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