Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10g20f
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18040.1 68417.m02685 eukaryotic translation initiation facto... 118 3e-27
At5g35620.2 68418.m04252 eukaryotic translation initiation facto... 88 5e-18
At5g35620.1 68418.m04251 eukaryotic translation initiation facto... 88 5e-18
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 85 3e-17
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 85 5e-17
At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identi... 77 8e-15
At5g47850.1 68418.m05912 protein kinase, putative contains simil... 29 2.7
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 29 3.5
At1g64590.1 68414.m07321 short-chain dehydrogenase/reductase (SD... 28 6.1
At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR... 27 8.1
At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR... 27 8.1
At1g08470.1 68414.m00938 strictosidine synthase family protein s... 27 8.1
>At4g18040.1 68417.m02685 eukaryotic translation initiation factor
4E 1 / eIF-4E1 / mRNA cap-binding protein 1 (EIF4E1)
identical to SP|O23252 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-4F 25 kDa subunit) (eIF-4F P26 subunit)
{Arabidopsis thaliana}
Length = 235
Score = 118 bits (284), Expect = 3e-27
Identities = 57/140 (40%), Positives = 79/140 (56%), Gaps = 4/140 (2%)
Frame = +1
Query: 115 DGDTSLQSESSPAIKHPLEHTWSYWL----YTNKSKEWIHNLVELSTFATVEDYWCLYHY 282
DG+ S+S HPLEH+W++W +K W +L + TF+TVE++W LY+
Sbjct: 45 DGNVDESSKSGVPESHPLEHSWTFWFDNPAVKSKQTSWGSSLRPVFTFSTVEEFWSLYNN 104
Query: 283 MKLPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGE 462
MK PS+L HG D+ FK I+P WEDPI GG+W + K + +IGE
Sbjct: 105 MKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKSD--KSWLYTLLALIGE 162
Query: 463 NFEHPDLICGVVVNIKAKSK 522
F+H D ICG VVNI+ K +
Sbjct: 163 QFDHGDEICGAVVNIRGKQE 182
>At5g35620.2 68418.m04252 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 167
Score = 87.8 bits (208), Expect = 5e-18
Identities = 47/135 (34%), Positives = 71/135 (52%), Gaps = 4/135 (2%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE---WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMIF 330
H LE WS+W + N+SK+ W +L + TF TVED+W L+ + S+L + +F
Sbjct: 26 HKLERKWSFW-FDNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 331 KKGIQPTWEDPINEKGGRWI-ICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
K G++P WEDP GG+W + + +IGE F+ D ICGVV ++
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGEQFDEADEICGVVASV 144
Query: 508 KAKSKICDIMDTW*R 552
+ +SK D + W R
Sbjct: 145 RPQSK-QDKLSLWTR 158
>At5g35620.1 68418.m04251 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 198
Score = 87.8 bits (208), Expect = 5e-18
Identities = 47/135 (34%), Positives = 71/135 (52%), Gaps = 4/135 (2%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE---WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMIF 330
H LE WS+W + N+SK+ W +L + TF TVED+W L+ + S+L + +F
Sbjct: 26 HKLERKWSFW-FDNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 331 KKGIQPTWEDPINEKGGRWI-ICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
K G++P WEDP GG+W + + +IGE F+ D ICGVV ++
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGEQFDEADEICGVVASV 144
Query: 508 KAKSKICDIMDTW*R 552
+ +SK D + W R
Sbjct: 145 RPQSK-QDKLSLWTR 158
Score = 28.7 bits (61), Expect = 3.5
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +2
Query: 515 KAKYVTLWTRGEDKQKN-MKIGKTVKEKLNINRNISYFNHN 634
K ++LWTR + + M IGK KE L++ I++ NH+
Sbjct: 149 KQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHD 189
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 85.4 bits (202), Expect = 3e-17
Identities = 41/123 (33%), Positives = 64/123 (52%), Gaps = 4/123 (3%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE----WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 327
H +++W++W SK W +L L TF T+E++W LY+ + P++ G D
Sbjct: 65 HCFQNSWTFWFDNPSSKSNQVIWGSSLRSLYTFGTIEEFWSLYNNIHPPTKWVSGADLYC 124
Query: 328 FKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
FK I+P WEDPI GG+W + K+ ++GE F+ D ICG V+N
Sbjct: 125 FKDKIEPKWEDPICANGGKWSMMFPKA--TLECNWLNTLLALVGEQFDQGDEICGAVLNF 182
Query: 508 KAK 516
+A+
Sbjct: 183 RAR 185
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 84.6 bits (200), Expect = 5e-17
Identities = 40/123 (32%), Positives = 64/123 (52%), Gaps = 4/123 (3%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE----WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 327
H +++W++W SK W +L L TFAT+E++W LY+ + P++ G D
Sbjct: 110 HCFQNSWTFWFDNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNIHPPTKWVSGSDLYC 169
Query: 328 FKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
FK I+P WEDPI GG+W + ++ ++GE F+ D ICG V+N
Sbjct: 170 FKDKIEPKWEDPICANGGKWTMFFPRA--TLESNWLNTLLALVGEQFDQGDEICGAVLNF 227
Query: 508 KAK 516
+ +
Sbjct: 228 RTR 230
>At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identical
to novel cap-binding protein nCBP [Arabidopsis thaliana]
GI:3108209; contains Pfam profile PF01652: Eukaryotic
initiation factor 4E
Length = 221
Score = 77.4 bits (182), Expect = 8e-15
Identities = 50/170 (29%), Positives = 80/170 (47%), Gaps = 7/170 (4%)
Frame = +1
Query: 82 MSALSSQHETRDGDTSLQSESSPAIKHPLEHTWSYWLYTNKS-----KEWIHNLVELSTF 246
M ++ S + T +S HPL + +S W YT ++ + + N+ ++ F
Sbjct: 17 MDSIKSHYVTDSVSEERRSRELKDGDHPLRYKFSIW-YTRRTPGVRNQSYEDNIKKMVEF 75
Query: 247 ATVEDYWCLYHYMKLPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESK--SARNX 420
+TVE +W Y ++ S L D FK GI+P WED N GG+WII SK SAR
Sbjct: 76 STVEGFWACYCHLARSSLLPSPTDLHFFKDGIRPLWEDGANCNGGKWIIRFSKVVSAR-- 133
Query: 421 XXXXXXXXXXMIGENFEHPDLICGVVVNIKAKSKICDIMDTW*RQTEKYE 570
++G+ + D ICG V++++ DI+ W R ++
Sbjct: 134 --FWEDLLLALVGDQLDDADNICGAVLSVRFNE---DIISVWNRNASDHQ 178
>At5g47850.1 68418.m05912 protein kinase, putative contains
similarity to cytokinin-regulated kinase 1 [Nicotiana
tabacum] gi|10998537|gb|AAG25966; contains protein
kinase domain, Pfam:PF00069
Length = 751
Score = 29.1 bits (62), Expect = 2.7
Identities = 22/53 (41%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = -2
Query: 614 CFC*YSISLLLFCRSSYFSVC--LHHVSIMSHILLFALILTTTPQIKSGCSKF 462
CF Y +SLLL SS+ VC L VSI SHI L+ CS F
Sbjct: 9 CFSSYFVSLLLLVLSSFSFVCFSLSTVSI-SHISNQTLVCALNNHSYLQCSSF 60
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +1
Query: 106 ETRDGDTSLQSESSPAIKHPLEHTWSYWLYTNKSKE-WIHNLVELSTFATVEDY 264
ET+ D Q+E K +E W W TN+++ W+ N E++T E Y
Sbjct: 323 ETKKDDQDDQTEKKKKTKKVVERYWD-WELTNETQPIWLRNPKEVTTAEYNEFY 375
>At1g64590.1 68414.m07321 short-chain dehydrogenase/reductase (SDR)
family protein contains INTERPRO family IPR002198
short-chain dehydrogenase/reductase (SDR) superfamily
Length = 334
Score = 27.9 bits (59), Expect = 6.1
Identities = 17/55 (30%), Positives = 29/55 (52%), Gaps = 4/55 (7%)
Frame = -2
Query: 503 LTTTPQIKSGCSKFSPIISRIRSNHRGS-RLRADLL---SQIIQRPPFSLIGSSH 351
+ T+P++++ C K+ + RS+ GS L+A L S ++ PP S SH
Sbjct: 277 VATSPRLRNVCGKYFSDCNEARSSKSGSCNLKAQRLWTASDLLVSPPNSTTNLSH 331
>At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1049
Score = 27.5 bits (58), Expect = 8.1
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +1
Query: 262 YWCLYHYMKLPSELNHGQ--DYMIFKKGIQPTWEDPINEKGGRWI-ICESKSARN 417
+W Y Y LPS+ + + D + I+ WED N + RW+ + +SK N
Sbjct: 592 HWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLN 646
>At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1049
Score = 27.5 bits (58), Expect = 8.1
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +1
Query: 262 YWCLYHYMKLPSELNHGQ--DYMIFKKGIQPTWEDPINEKGGRWI-ICESKSARN 417
+W Y Y LPS+ + + D + I+ WED N + RW+ + +SK N
Sbjct: 592 HWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLN 646
>At1g08470.1 68414.m00938 strictosidine synthase family protein
similar to strictosidine synthase [Rauvolfia
serpentina][SP|P15324]
Length = 390
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +2
Query: 485 FAALSSILKRKAKYVTLWTRGEDKQKNMKIGKTVKEKLNINRNISYFN 628
F SS +R+ K++ L GED + +K KE + RN+ + N
Sbjct: 191 FTDSSSFFQRR-KFMLLIVSGEDSGRVLKYNPKTKETTTLVRNLQFPN 237
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,542,833
Number of Sequences: 28952
Number of extensions: 278411
Number of successful extensions: 765
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 741
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 757
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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