BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10g14f (637 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 67 2e-12 SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb... 67 2e-12 SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 63 4e-11 SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pomb... 29 0.74 SPAC22H10.08 |||DUF2009 protein|Schizosaccharomyces pombe|chr 1|... 27 1.7 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 4.0 SPBC21C3.06 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 5.2 SPBC947.12 |kms2||spindle pole body protein Kms2|Schizosaccharom... 25 6.9 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 25 6.9 SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schi... 25 9.1 SPAC23C4.16c |atg15||triacylglycerol lipase Atg15 |Schizosacchar... 25 9.1 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 66.9 bits (156), Expect = 2e-12 Identities = 47/155 (30%), Positives = 75/155 (48%), Gaps = 5/155 (3%) Frame = +1 Query: 64 LAVLFSKSVSGQFGPFEQDFGYVTVREGAHMFWWLFYTTAPVEKYTERPLIVWLQXXXXX 243 L V SK S +Q GY+ V + H+F+W F + E P+++WL Sbjct: 571 LRVKDSKPESLGIDTVKQYTGYLDVEDDRHLFFWFFESRNDPEN---DPVVLWLNGGPGC 627 Query: 244 XXXXXXNFEILGPLDLSLQ----ERN-HSWVKNFNVLFVDNPVGTGFSYVDNVMQLTKTN 408 E LGP ++++ E N HSW N +V+F+D P+ TGFS D+ + T T Sbjct: 628 SSLTGLFME-LGPSSINIETLKPEYNPHSWNSNASVIFLDQPINTGFSNGDDSVLDTVTA 686 Query: 409 DEIALDFVELMRGFYNRRPEFNDVPLYIYGQSYGG 513 + F+ L F+ + P++ + +I G+SY G Sbjct: 687 GKDVYAFLNL---FFAKFPQYAHLDFHIAGESYAG 718 >SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 510 Score = 66.9 bits (156), Expect = 2e-12 Identities = 47/160 (29%), Positives = 74/160 (46%), Gaps = 4/160 (2%) Frame = +1 Query: 154 MFWWLFYTTAPVEKYTERPLIVWLQXXXXXXXXXXXNFEILGPLDL----SLQERNHSWV 321 +F+W+F + P +Y R I+WL E+ GP L + Q W Sbjct: 60 LFFWMFESVKP--EYEHRS-ILWLNGGPGCSSEDGSLMEV-GPFRLDDNNTFQLNPGRWD 115 Query: 322 KNFNVLFVDNPVGTGFSYVDNVMQLTKTNDEIALDFVELMRGFYNRRPEFNDVPLYIYGQ 501 + N+LFVD P+GTG+SY N+++A DF F PE + +I G+ Sbjct: 116 ELGNLLFVDQPLGTGYSY-SLAKDFQSNNEKMANDFSIFFEKFLEEFPERANDEWFIAGE 174 Query: 502 SYGGKMAIDMGLRMHEAEKAGTIRSNLKGIAMGNAWISPV 621 S+ G+ + ++ E NL G+A+GN WI+P+ Sbjct: 175 SFAGQYIPHIAAKLKEKNLV-----NLGGLAIGNGWINPL 209 >SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 62.9 bits (146), Expect = 4e-11 Identities = 41/120 (34%), Positives = 57/120 (47%), Gaps = 5/120 (4%) Frame = +1 Query: 166 LFYTTAPVEKYTERPLIVWLQXXXXXXXXXXXNFEILGPLDLSLQERN-----HSWVKNF 330 LFYT AP +E IVWLQ F GP+++S + SW Sbjct: 87 LFYTYAPAVVDSET-FIVWLQGGPGCAGTLGF-FSENGPIEISQSSPSPSLNPESWTNFA 144 Query: 331 NVLFVDNPVGTGFSYVDNVMQLTKTNDEIALDFVELMRGFYNRRPEFNDVPLYIYGQSYG 510 N+L++D P GTG+S T T +E + DFV ++ FY + P LY+ G+SYG Sbjct: 145 NMLWLDQPFGTGYS--QGQAAYTTTIEEASSDFVNALKSFYQKFPHLMKKKLYLVGESYG 202 >SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pombe|chr 2|||Manual Length = 600 Score = 28.7 bits (61), Expect = 0.74 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = -2 Query: 378 NITKASAYRIINEQNIEILHP 316 +I KA R+ EQN+E++HP Sbjct: 218 DIAKAECARLAKEQNLEVIHP 238 >SPAC22H10.08 |||DUF2009 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 27.5 bits (58), Expect = 1.7 Identities = 12/47 (25%), Positives = 23/47 (48%) Frame = +1 Query: 298 QERNHSWVKNFNVLFVDNPVGTGFSYVDNVMQLTKTNDEIALDFVEL 438 QE++ +W+ + + D V ++D MQ+ + + L F EL Sbjct: 362 QEQSETWIGSSTIHLGDTAVPNALLFIDKYMQVPRILTPLVLLFKEL 408 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.2 bits (55), Expect = 4.0 Identities = 12/34 (35%), Positives = 14/34 (41%) Frame = +1 Query: 85 SVSGQFGPFEQDFGYVTVREGAHMFWWLFYTTAP 186 S+ GPF + F V A WWL T P Sbjct: 1350 SIVSTTGPFNESFSAKPVEPCASEKWWLNSTAVP 1383 >SPBC21C3.06 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 122 Score = 25.8 bits (54), Expect = 5.2 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +2 Query: 443 VDSTIDVLSSTMFLSTFTVSRTVVKWP 523 VD+ + SST ST+ + RT++K P Sbjct: 12 VDAIFAISSSTFLWSTWVLQRTILKRP 38 >SPBC947.12 |kms2||spindle pole body protein Kms2|Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 25.4 bits (53), Expect = 6.9 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = +1 Query: 145 GAHMFWWLFYTTAPVEKYTERPLIVWL 225 G H+F++LF+ A ++++ P + WL Sbjct: 422 GIHIFYFLFFHVATIQQW---PFLFWL 445 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 25.4 bits (53), Expect = 6.9 Identities = 9/30 (30%), Positives = 19/30 (63%) Frame = +1 Query: 352 PVGTGFSYVDNVMQLTKTNDEIALDFVELM 441 P+ + FSY ++ LTK E +++F++ + Sbjct: 721 PLNSMFSYSSDIRALTKGKGEFSMEFLKYL 750 >SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/26 (42%), Positives = 11/26 (42%) Frame = -2 Query: 222 PNDEWTFSIFLYWSCCVE*PPKHVSS 145 PND W I L CC P K S Sbjct: 219 PNDVWALGIILINLCCKRNPWKRACS 244 >SPAC23C4.16c |atg15||triacylglycerol lipase Atg15 |Schizosaccharomyces pombe|chr 1|||Manual Length = 424 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = +1 Query: 448 FYNRRPEFNDVPLYIYGQSYGGKMAIDMGL 537 FY+ + + D +++ G S GG A MGL Sbjct: 265 FYSVKELYPDAQIWLTGHSLGGATAALMGL 294 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,651,502 Number of Sequences: 5004 Number of extensions: 54735 Number of successful extensions: 117 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 110 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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