SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10f21r
         (757 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...   105   7e-24
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    87   2e-18
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    68   1e-12
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa...    48   1e-06
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    30   0.31 
SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces p...    28   1.7  
SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch...    26   5.0  
SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|...    26   5.0  
SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha...    26   6.7  
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr...    26   6.7  

>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score =  105 bits (252), Expect = 7e-24
 Identities = 63/203 (31%), Positives = 110/203 (54%), Gaps = 4/203 (1%)
 Frame = -1

Query: 727 ASALYFHIAEGERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDP-- 554
           + A++F+    + KCF++++P    V    K E ++ ++  ++ T+  I + V+V +   
Sbjct: 17  SQAVHFYFDGSKPKCFLKDMPKGMMVTGIVKAEQWNNEANKWV-TSNNIKIRVQVDETFA 75

Query: 553 NDRILLSRVYSLEGMISFTSSTPGEHVICMYSNST-SWFSGSQLRVHLDIQVGEHAVDYA 377
           N+ ++          + FT++  G H IC  S S  +WFS ++ R+H+D+   +    Y 
Sbjct: 76  NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDK---YN 132

Query: 376 NVAQKE-KLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTG 200
            +A  E +  +L  R+  L  ++  I  EQ  QR RE  FR TSES N RV+ W+++Q  
Sbjct: 133 LIASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIV 192

Query: 199 VLVIIGYWQMRHLKSFFEAKKLV 131
           VL++   WQ+ HL+ FF  +KLV
Sbjct: 193 VLLVTCIWQLSHLQRFFVKEKLV 215


>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 87.4 bits (207), Expect = 2e-18
 Identities = 56/188 (29%), Positives = 95/188 (50%)
 Frame = -1

Query: 694 ERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSRVYSLE 515
           +R+CF E + ++  + V Y+  +     GG    +      + + +P  +I+   V +  
Sbjct: 30  QRECFYENLRNNDQMSVTYQTNV-----GGDQLVS------MSIYNPAGQIMHQEVPNSM 78

Query: 514 GMISFTSSTPGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVAQKEKLTELQLR 335
              SFT   PG+++ C Y+++     G    V  ++    H V Y +    +    L  +
Sbjct: 79  AQYSFTVKNPGKYMYCFYNDA---LDGESKEVLFNV----HGVIYISDEDLDANNPLLGK 131

Query: 334 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVIIGYWQMRHLKS 155
           +RQL D + ++  EQ Y   RE   R T+ESTN RV WWS+LQT +LV +  +Q+ +LK 
Sbjct: 132 VRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLKR 191

Query: 154 FFEAKKLV 131
            FE K++V
Sbjct: 192 LFEVKRVV 199


>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 53/188 (28%), Positives = 86/188 (45%), Gaps = 3/188 (1%)
 Frame = -1

Query: 685 CFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSRVYSLEGMI 506
           C  E + +   VIVN K         G M     + M +     N    +  V   E  +
Sbjct: 36  CLREYVGEKNLVIVNLKTT-------GNMGDGQTLSMMITDSSGNTHSSIQNVLG-EKSV 87

Query: 505 SFTSSTPGEHVICMYSNSTSWFSGSQLR---VHLDIQVGEHAVDYANVAQKEKLTELQLR 335
           +F         IC  +  T     S+ +   V L+  VG  A DY+++ +   L  ++  
Sbjct: 88  AFDVDASAMLDICFLNTLTPGAIESEHKKRSVKLEFTVGADADDYSSLQKANNLEPVEAD 147

Query: 334 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVIIGYWQMRHLKS 155
           IR+  D + +I  +  Y + RE RFR T+ESTN+RV  ++ L    L ++  WQ+ +L+S
Sbjct: 148 IRRARDFIEEIKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVLVIWQILYLRS 207

Query: 154 FFEAKKLV 131
           FF+ K L+
Sbjct: 208 FFQRKHLI 215


>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 46/208 (22%), Positives = 95/208 (45%), Gaps = 5/208 (2%)
 Frame = -1

Query: 754 LVIALLADCASA--LYFHIAEGERKCF-IEEIPDDTTVIVNYKVELYDPKSGGFMQTTPG 584
           + + LLA  ASA  L F +   E++C+ ++           Y V+     SGG       
Sbjct: 9   ITLFLLASLASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQ-----SGG------S 57

Query: 583 IGMHVEVRDPNDRILLSRVYSLEGMISFTSSTPGEHVICMYSNSTSWFSGS--QLRVHLD 410
             +   ++ P+ + +       +  + FT    GE+  C + N  S F+     + + ++
Sbjct: 58  FDVDYMIKAPSQKTVALGKKRRQADVFFTLEEKGEYEFC-FDNHMSTFTDKIVTMEITME 116

Query: 409 IQVGEHAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQR 230
            ++   A+   + A+  K   +Q  + ++   + +I + Q Y + RE R   T +ST  R
Sbjct: 117 NELSLPALT-RDEAKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQAR 175

Query: 229 VLWWSLLQTGVLVIIGYWQMRHLKSFFE 146
           + W+SL ++ ++V +   Q+  +K+FF+
Sbjct: 176 IFWFSLAESIMVVALSALQVFIVKTFFK 203


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 30.3 bits (65), Expect = 0.31
 Identities = 12/40 (30%), Positives = 23/40 (57%)
 Frame = -2

Query: 321 WIKYTRSQRSRLTRGKEKSVFDKHQKAQTSECSGGVCYRQ 202
           W+  + S+R  L + ++++V  K ++ Q   CS  VC R+
Sbjct: 266 WLSLSESERRSLVKVEKEAVLQKMKEQQKYSCSCSVCGRK 305


>SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 392

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = -1

Query: 685 CFIEEIPDDTTVI-VNYKVELYDPKSGGFMQTTPGIGMHV 569
           C++ ++ DDT ++ +NY +  Y PK     Q   GI + V
Sbjct: 49  CYMFKLMDDTGIVELNYSLSKYSPKFEMLRQLQLGISVFV 88


>SPAC4F10.18 |||WD repeat protein, human NUP37
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 391

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 14/50 (28%), Positives = 26/50 (52%)
 Frame = -1

Query: 679 IEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSR 530
           I  + DD T+I+ +++    P   G+  ++PGI +     +PN  I+  R
Sbjct: 157 IASVGDDCTLII-WRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGER 205


>SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 785

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = -3

Query: 248 RKHKPASALVEFATDRCPCHHRLLADETSQEL 153
           RKH+  + +V    DR  C H  L  E +++L
Sbjct: 716 RKHQADACIVRVMKDRKVCEHNQLMAEVTRQL 747


>SPAC20G8.08c |fft1||fun thirty related protein
           Fft1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 944

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = -1

Query: 676 EEIPDDTTVIVNYKVELYDPKSGGF 602
           +++ DD     NYKV L   KSGGF
Sbjct: 820 QQLIDDFHTNENYKVFLLSTKSGGF 844


>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 661

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +3

Query: 489 VLDVNEIIPSNE*TRDNSILSFGSRTS 569
           V D+ EI+PS   T  NS+   GS T+
Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTT 559


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,925,427
Number of Sequences: 5004
Number of extensions: 56764
Number of successful extensions: 167
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -