BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10f21r (757 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 105 7e-24 SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 87 2e-18 SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 68 1e-12 SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 48 1e-06 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 30 0.31 SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces p... 28 1.7 SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch... 26 5.0 SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|... 26 5.0 SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha... 26 6.7 SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 26 6.7 >SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 105 bits (252), Expect = 7e-24 Identities = 63/203 (31%), Positives = 110/203 (54%), Gaps = 4/203 (1%) Frame = -1 Query: 727 ASALYFHIAEGERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDP-- 554 + A++F+ + KCF++++P V K E ++ ++ ++ T+ I + V+V + Sbjct: 17 SQAVHFYFDGSKPKCFLKDMPKGMMVTGIVKAEQWNNEANKWV-TSNNIKIRVQVDETFA 75 Query: 553 NDRILLSRVYSLEGMISFTSSTPGEHVICMYSNST-SWFSGSQLRVHLDIQVGEHAVDYA 377 N+ ++ + FT++ G H IC S S +WFS ++ R+H+D+ + Y Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDK---YN 132 Query: 376 NVAQKE-KLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTG 200 +A E + +L R+ L ++ I EQ QR RE FR TSES N RV+ W+++Q Sbjct: 133 LIASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIV 192 Query: 199 VLVIIGYWQMRHLKSFFEAKKLV 131 VL++ WQ+ HL+ FF +KLV Sbjct: 193 VLLVTCIWQLSHLQRFFVKEKLV 215 >SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schizosaccharomyces pombe|chr 3|||Manual Length = 199 Score = 87.4 bits (207), Expect = 2e-18 Identities = 56/188 (29%), Positives = 95/188 (50%) Frame = -1 Query: 694 ERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSRVYSLE 515 +R+CF E + ++ + V Y+ + GG + + + +P +I+ V + Sbjct: 30 QRECFYENLRNNDQMSVTYQTNV-----GGDQLVS------MSIYNPAGQIMHQEVPNSM 78 Query: 514 GMISFTSSTPGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVAQKEKLTELQLR 335 SFT PG+++ C Y+++ G V ++ H V Y + + L + Sbjct: 79 AQYSFTVKNPGKYMYCFYNDA---LDGESKEVLFNV----HGVIYISDEDLDANNPLLGK 131 Query: 334 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVIIGYWQMRHLKS 155 +RQL D + ++ EQ Y RE R T+ESTN RV WWS+LQT +LV + +Q+ +LK Sbjct: 132 VRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLKR 191 Query: 154 FFEAKKLV 131 FE K++V Sbjct: 192 LFEVKRVV 199 >SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 68.1 bits (159), Expect = 1e-12 Identities = 53/188 (28%), Positives = 86/188 (45%), Gaps = 3/188 (1%) Frame = -1 Query: 685 CFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSRVYSLEGMI 506 C E + + VIVN K G M + M + N + V E + Sbjct: 36 CLREYVGEKNLVIVNLKTT-------GNMGDGQTLSMMITDSSGNTHSSIQNVLG-EKSV 87 Query: 505 SFTSSTPGEHVICMYSNSTSWFSGSQLR---VHLDIQVGEHAVDYANVAQKEKLTELQLR 335 +F IC + T S+ + V L+ VG A DY+++ + L ++ Sbjct: 88 AFDVDASAMLDICFLNTLTPGAIESEHKKRSVKLEFTVGADADDYSSLQKANNLEPVEAD 147 Query: 334 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVIIGYWQMRHLKS 155 IR+ D + +I + Y + RE RFR T+ESTN+RV ++ L L ++ WQ+ +L+S Sbjct: 148 IRRARDFIEEIKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVLVIWQILYLRS 207 Query: 154 FFEAKKLV 131 FF+ K L+ Sbjct: 208 FFQRKHLI 215 >SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 210 Score = 48.0 bits (109), Expect = 1e-06 Identities = 46/208 (22%), Positives = 95/208 (45%), Gaps = 5/208 (2%) Frame = -1 Query: 754 LVIALLADCASA--LYFHIAEGERKCF-IEEIPDDTTVIVNYKVELYDPKSGGFMQTTPG 584 + + LLA ASA L F + E++C+ ++ Y V+ SGG Sbjct: 9 ITLFLLASLASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQ-----SGG------S 57 Query: 583 IGMHVEVRDPNDRILLSRVYSLEGMISFTSSTPGEHVICMYSNSTSWFSGS--QLRVHLD 410 + ++ P+ + + + + FT GE+ C + N S F+ + + ++ Sbjct: 58 FDVDYMIKAPSQKTVALGKKRRQADVFFTLEEKGEYEFC-FDNHMSTFTDKIVTMEITME 116 Query: 409 IQVGEHAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQR 230 ++ A+ + A+ K +Q + ++ + +I + Q Y + RE R T +ST R Sbjct: 117 NELSLPALT-RDEAKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQAR 175 Query: 229 VLWWSLLQTGVLVIIGYWQMRHLKSFFE 146 + W+SL ++ ++V + Q+ +K+FF+ Sbjct: 176 IFWFSLAESIMVVALSALQVFIVKTFFK 203 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 30.3 bits (65), Expect = 0.31 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = -2 Query: 321 WIKYTRSQRSRLTRGKEKSVFDKHQKAQTSECSGGVCYRQ 202 W+ + S+R L + ++++V K ++ Q CS VC R+ Sbjct: 266 WLSLSESERRSLVKVEKEAVLQKMKEQQKYSCSCSVCGRK 305 >SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 27.9 bits (59), Expect = 1.7 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = -1 Query: 685 CFIEEIPDDTTVI-VNYKVELYDPKSGGFMQTTPGIGMHV 569 C++ ++ DDT ++ +NY + Y PK Q GI + V Sbjct: 49 CYMFKLMDDTGIVELNYSLSKYSPKFEMLRQLQLGISVFV 88 >SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 26.2 bits (55), Expect = 5.0 Identities = 14/50 (28%), Positives = 26/50 (52%) Frame = -1 Query: 679 IEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSR 530 I + DD T+I+ +++ P G+ ++PGI + +PN I+ R Sbjct: 157 IASVGDDCTLII-WRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGER 205 >SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 785 Score = 26.2 bits (55), Expect = 5.0 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = -3 Query: 248 RKHKPASALVEFATDRCPCHHRLLADETSQEL 153 RKH+ + +V DR C H L E +++L Sbjct: 716 RKHQADACIVRVMKDRKVCEHNQLMAEVTRQL 747 >SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccharomyces pombe|chr 1|||Manual Length = 944 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = -1 Query: 676 EEIPDDTTVIVNYKVELYDPKSGGF 602 +++ DD NYKV L KSGGF Sbjct: 820 QQLIDDFHTNENYKVFLLSTKSGGF 844 >SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 661 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 489 VLDVNEIIPSNE*TRDNSILSFGSRTS 569 V D+ EI+PS T NS+ GS T+ Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTT 559 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,925,427 Number of Sequences: 5004 Number of extensions: 56764 Number of successful extensions: 167 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 361294920 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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