SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10f21f
         (644 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    89   5e-19
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    74   2e-14
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    55   8e-09
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    30   0.25 
SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces p...    28   1.3  
SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch...    26   4.0  
SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha...    26   5.3  
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr...    26   5.3  
SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa...    25   9.3  
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb...    25   9.3  
SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc...    25   9.3  
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    25   9.3  

>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 89.0 bits (211), Expect = 5e-19
 Identities = 55/188 (29%), Positives = 99/188 (52%), Gaps = 4/188 (2%)
 Frame = +3

Query: 93  ASALYFHIAEGERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDP-- 266
           + A++F+    + KCF++++P    V    K E ++ ++  ++ T+  I + V+V +   
Sbjct: 17  SQAVHFYFDGSKPKCFLKDMPKGMMVTGIVKAEQWNNEANKWV-TSNNIKIRVQVDETFA 75

Query: 267 NDRILLSRVYSLEGMISFTSSTPGEHVICMYSNST-SWFSGSQLRVHLDIQVGEHAVDYA 443
           N+ ++          + FT++  G H IC  S S  +WFS ++ R+H+D+   +    Y 
Sbjct: 76  NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDK---YN 132

Query: 444 NVAQKE-KLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTG 620
            +A  E +  +L  R+  L  ++  I  EQ  QR RE  FR TSES N RV+ W+++Q  
Sbjct: 133 LIASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIV 192

Query: 621 VLVIIGYW 644
           VL++   W
Sbjct: 193 VLLVTCIW 200


>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 74.1 bits (174), Expect = 2e-14
 Identities = 49/170 (28%), Positives = 83/170 (48%)
 Frame = +3

Query: 126 ERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSRVYSLE 305
           +R+CF E + ++  + V Y+  +     GG    +      + + +P  +I+   V +  
Sbjct: 30  QRECFYENLRNNDQMSVTYQTNV-----GGDQLVS------MSIYNPAGQIMHQEVPNSM 78

Query: 306 GMISFTSSTPGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVAQKEKLTELQLR 485
              SFT   PG+++ C Y+++     G    V  ++    H V Y +    +    L  +
Sbjct: 79  AQYSFTVKNPGKYMYCFYNDA---LDGESKEVLFNV----HGVIYISDEDLDANNPLLGK 131

Query: 486 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVII 635
           +RQL D + ++  EQ Y   RE   R T+ESTN RV WWS+LQT +LV +
Sbjct: 132 VRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSV 181


>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 55.2 bits (127), Expect = 8e-09
 Identities = 55/202 (27%), Positives = 84/202 (41%), Gaps = 6/202 (2%)
 Frame = +3

Query: 57  SSLLVIALLADCASALYFHI---AEGERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQT 227
           SSL  +  L     AL F I      E  C  E + +   VIVN K         G M  
Sbjct: 7   SSLFFMLALLTVVHALNFDIPAKTNPEPFCLREYVGEKNLVIVNLKTT-------GNMGD 59

Query: 228 TPGIGMHVEVRDPNDRILLSRVYSLEGMISFTSSTPGEHVICMYSNSTSWFSGSQLR--- 398
              + M +     N    +  V   E  ++F         IC  +  T     S+ +   
Sbjct: 60  GQTLSMMITDSSGNTHSSIQNVLG-EKSVAFDVDASAMLDICFLNTLTPGAIESEHKKRS 118

Query: 399 VHLDIQVGEHAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSES 578
           V L+  VG  A DY+++ +   L  ++  IR+  D + +I  +  Y + RE RFR T+ES
Sbjct: 119 VKLEFTVGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNES 178

Query: 579 TNQRVLWWSLLQTGVLVIIGYW 644
           TN+RV  ++ L    L ++  W
Sbjct: 179 TNERVKNFAYLTFISLFVLVIW 200


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 12/40 (30%), Positives = 23/40 (57%)
 Frame = +1

Query: 499 WIKYTRSQRSRLTRGKEKSVFDKHQKAQTSECSGGVCYRQ 618
           W+  + S+R  L + ++++V  K ++ Q   CS  VC R+
Sbjct: 266 WLSLSESERRSLVKVEKEAVLQKMKEQQKYSCSCSVCGRK 305


>SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 392

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +3

Query: 135 CFIEEIPDDTTVI-VNYKVELYDPKSGGFMQTTPGIGMHV 251
           C++ ++ DDT ++ +NY +  Y PK     Q   GI + V
Sbjct: 49  CYMFKLMDDTGIVELNYSLSKYSPKFEMLRQLQLGISVFV 88


>SPAC4F10.18 |||WD repeat protein, human NUP37
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 391

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 14/50 (28%), Positives = 26/50 (52%)
 Frame = +3

Query: 141 IEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSR 290
           I  + DD T+I+ +++    P   G+  ++PGI +     +PN  I+  R
Sbjct: 157 IASVGDDCTLII-WRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGER 205


>SPAC20G8.08c |fft1||fun thirty related protein
           Fft1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 944

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +3

Query: 144 EEIPDDTTVIVNYKVELYDPKSGGF 218
           +++ DD     NYKV L   KSGGF
Sbjct: 820 QQLIDDFHTNENYKVFLLSTKSGGF 844


>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 661

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = -2

Query: 331 VLDVNEIIPSNE*TRDNSILSFGSRTS 251
           V D+ EI+PS   T  NS+   GS T+
Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTT 559


>SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 618

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
 Frame = +3

Query: 426 HAVDYANVAQKEKLTELQLRIRQL--LDQVHQITKEQAYQ 539
           +AVDY   + K+K+ +L+  I +L   D V  +  E  Y+
Sbjct: 147 NAVDYIIQSAKDKVQKLEAEIEELSTADDVDDVLLESKYE 186


>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 398

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = +3

Query: 270 DRILLSRVYSLEGMISFTSS 329
           DR+ LS +Y+L+ ++ F SS
Sbjct: 170 DRVFLSEIYALKILVEFLSS 189


>SPAC1687.03c |rfc4||DNA replication factor C complex subunit
           Rfc4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 342

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 16/71 (22%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
 Frame = +3

Query: 333 PGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVA-QKEKLTELQLRIRQLLDQV 509
           PG H I +   + S  +G+Q  +   +++  +   +A    Q  K+ E  ++ R  + + 
Sbjct: 117 PGRHKIIILDEADSMTAGAQQALRRTMEIYSNTTRFALACNQSNKIIE-PIQSRCAILRY 175

Query: 510 HQITKEQAYQR 542
            ++T +Q  QR
Sbjct: 176 SRLTDQQVLQR 186


>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 673

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 10/35 (28%), Positives = 20/35 (57%)
 Frame = +3

Query: 504 QVHQITKEQAYQRQREERFRQTSESTNQRVLWWSL 608
           ++ +  K++  Q  REER+RQ +E   +  ++  L
Sbjct: 535 EISKRRKQRKAQEMREERYRQRAERDTEEQIYSEL 569


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,680,323
Number of Sequences: 5004
Number of extensions: 52689
Number of successful extensions: 155
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -