BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10f21f (644 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 89 5e-19 SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 74 2e-14 SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 55 8e-09 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 30 0.25 SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces p... 28 1.3 SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch... 26 4.0 SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha... 26 5.3 SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 26 5.3 SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa... 25 9.3 SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.3 SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc... 25 9.3 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.3 >SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 89.0 bits (211), Expect = 5e-19 Identities = 55/188 (29%), Positives = 99/188 (52%), Gaps = 4/188 (2%) Frame = +3 Query: 93 ASALYFHIAEGERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDP-- 266 + A++F+ + KCF++++P V K E ++ ++ ++ T+ I + V+V + Sbjct: 17 SQAVHFYFDGSKPKCFLKDMPKGMMVTGIVKAEQWNNEANKWV-TSNNIKIRVQVDETFA 75 Query: 267 NDRILLSRVYSLEGMISFTSSTPGEHVICMYSNST-SWFSGSQLRVHLDIQVGEHAVDYA 443 N+ ++ + FT++ G H IC S S +WFS ++ R+H+D+ + Y Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDK---YN 132 Query: 444 NVAQKE-KLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTG 620 +A E + +L R+ L ++ I EQ QR RE FR TSES N RV+ W+++Q Sbjct: 133 LIASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIV 192 Query: 621 VLVIIGYW 644 VL++ W Sbjct: 193 VLLVTCIW 200 >SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schizosaccharomyces pombe|chr 3|||Manual Length = 199 Score = 74.1 bits (174), Expect = 2e-14 Identities = 49/170 (28%), Positives = 83/170 (48%) Frame = +3 Query: 126 ERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSRVYSLE 305 +R+CF E + ++ + V Y+ + GG + + + +P +I+ V + Sbjct: 30 QRECFYENLRNNDQMSVTYQTNV-----GGDQLVS------MSIYNPAGQIMHQEVPNSM 78 Query: 306 GMISFTSSTPGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVAQKEKLTELQLR 485 SFT PG+++ C Y+++ G V ++ H V Y + + L + Sbjct: 79 AQYSFTVKNPGKYMYCFYNDA---LDGESKEVLFNV----HGVIYISDEDLDANNPLLGK 131 Query: 486 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVII 635 +RQL D + ++ EQ Y RE R T+ESTN RV WWS+LQT +LV + Sbjct: 132 VRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSV 181 >SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 55.2 bits (127), Expect = 8e-09 Identities = 55/202 (27%), Positives = 84/202 (41%), Gaps = 6/202 (2%) Frame = +3 Query: 57 SSLLVIALLADCASALYFHI---AEGERKCFIEEIPDDTTVIVNYKVELYDPKSGGFMQT 227 SSL + L AL F I E C E + + VIVN K G M Sbjct: 7 SSLFFMLALLTVVHALNFDIPAKTNPEPFCLREYVGEKNLVIVNLKTT-------GNMGD 59 Query: 228 TPGIGMHVEVRDPNDRILLSRVYSLEGMISFTSSTPGEHVICMYSNSTSWFSGSQLR--- 398 + M + N + V E ++F IC + T S+ + Sbjct: 60 GQTLSMMITDSSGNTHSSIQNVLG-EKSVAFDVDASAMLDICFLNTLTPGAIESEHKKRS 118 Query: 399 VHLDIQVGEHAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSES 578 V L+ VG A DY+++ + L ++ IR+ D + +I + Y + RE RFR T+ES Sbjct: 119 VKLEFTVGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNES 178 Query: 579 TNQRVLWWSLLQTGVLVIIGYW 644 TN+RV ++ L L ++ W Sbjct: 179 TNERVKNFAYLTFISLFVLVIW 200 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 30.3 bits (65), Expect = 0.25 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = +1 Query: 499 WIKYTRSQRSRLTRGKEKSVFDKHQKAQTSECSGGVCYRQ 618 W+ + S+R L + ++++V K ++ Q CS VC R+ Sbjct: 266 WLSLSESERRSLVKVEKEAVLQKMKEQQKYSCSCSVCGRK 305 >SPAP27G11.08c |meu32|mug11|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +3 Query: 135 CFIEEIPDDTTVI-VNYKVELYDPKSGGFMQTTPGIGMHV 251 C++ ++ DDT ++ +NY + Y PK Q GI + V Sbjct: 49 CYMFKLMDDTGIVELNYSLSKYSPKFEMLRQLQLGISVFV 88 >SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 26.2 bits (55), Expect = 4.0 Identities = 14/50 (28%), Positives = 26/50 (52%) Frame = +3 Query: 141 IEEIPDDTTVIVNYKVELYDPKSGGFMQTTPGIGMHVEVRDPNDRILLSR 290 I + DD T+I+ +++ P G+ ++PGI + +PN I+ R Sbjct: 157 IASVGDDCTLII-WRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGER 205 >SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccharomyces pombe|chr 1|||Manual Length = 944 Score = 25.8 bits (54), Expect = 5.3 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +3 Query: 144 EEIPDDTTVIVNYKVELYDPKSGGF 218 +++ DD NYKV L KSGGF Sbjct: 820 QQLIDDFHTNENYKVFLLSTKSGGF 844 >SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 661 Score = 25.8 bits (54), Expect = 5.3 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -2 Query: 331 VLDVNEIIPSNE*TRDNSILSFGSRTS 251 V D+ EI+PS T NS+ GS T+ Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTT 559 >SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 25.0 bits (52), Expect = 9.3 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Frame = +3 Query: 426 HAVDYANVAQKEKLTELQLRIRQL--LDQVHQITKEQAYQ 539 +AVDY + K+K+ +L+ I +L D V + E Y+ Sbjct: 147 NAVDYIIQSAKDKVQKLEAEIEELSTADDVDDVLLESKYE 186 >SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 25.0 bits (52), Expect = 9.3 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = +3 Query: 270 DRILLSRVYSLEGMISFTSS 329 DR+ LS +Y+L+ ++ F SS Sbjct: 170 DRVFLSEIYALKILVEFLSS 189 >SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 342 Score = 25.0 bits (52), Expect = 9.3 Identities = 16/71 (22%), Positives = 34/71 (47%), Gaps = 1/71 (1%) Frame = +3 Query: 333 PGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVA-QKEKLTELQLRIRQLLDQV 509 PG H I + + S +G+Q + +++ + +A Q K+ E ++ R + + Sbjct: 117 PGRHKIIILDEADSMTAGAQQALRRTMEIYSNTTRFALACNQSNKIIE-PIQSRCAILRY 175 Query: 510 HQITKEQAYQR 542 ++T +Q QR Sbjct: 176 SRLTDQQVLQR 186 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/35 (28%), Positives = 20/35 (57%) Frame = +3 Query: 504 QVHQITKEQAYQRQREERFRQTSESTNQRVLWWSL 608 ++ + K++ Q REER+RQ +E + ++ L Sbjct: 535 EISKRRKQRKAQEMREERYRQRAERDTEEQIYSEL 569 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,680,323 Number of Sequences: 5004 Number of extensions: 52689 Number of successful extensions: 155 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 289756512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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