Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10f10f
(544 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 111 8e-26
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 81 8e-17
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 58 8e-10
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 44 2e-05
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 38 0.001
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 38 0.001
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.005
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.0
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 26 3.1
SPBP16F5.05c |||ribosome biogenesis protein Nop8|Schizosaccharom... 25 5.5
SPAC664.07c |rad9||checkpoint clamp complex protein Rad9|Schizos... 25 9.5
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 111 bits (266), Expect = 8e-26
Identities = 52/123 (42%), Positives = 77/123 (62%)
Frame = +3
Query: 138 YLCKAAEEDVLDLTDSDFSAXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPP 317
+ C +AE V + + ++ +V FYAPWCGHCK L PEY AA L+ D
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKD--G 72
Query: 318 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSS 497
++L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+
Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131
Query: 498 KEL 506
K +
Sbjct: 132 KPI 134
Score = 64.1 bits (149), Expect = 1e-11
Identities = 41/122 (33%), Positives = 59/122 (48%), Gaps = 5/122 (4%)
Frame = +3
Query: 153 AEEDVLDLTDSDFS-AXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALA 329
++ED++ L +F + + LV FYAPWCGHCK L P Y A D V +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDD-SNVVVA 411
Query: 330 KVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM--RAQVGPSS 497
K+D TE S S+SG+PT+ F+ + + Y G R + ++ A P
Sbjct: 412 KIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFEPIK 467
Query: 498 KE 503
KE
Sbjct: 468 KE 469
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 81.4 bits (192), Expect = 8e-17
Identities = 48/152 (31%), Positives = 79/152 (51%), Gaps = 6/152 (3%)
Frame = +3
Query: 75 KAPAKFKMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAXLSQHDTALVMFYAPWCGH 254
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 255 CKRLKPEYAVAAGLLKTDVPPVALAKVDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE- 419
CK+L P Y A L + +P A VDC + ++ C Q+ V G+PT+K+ KG
Sbjct: 63 CKKLVPTYQKLASNLHSLLPVTA---VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSS 119
Query: 420 -LSSEYNGPRESNGIVKYMRAQVGPSSKELLT 512
S++YNG R + K++ + PS ++LT
Sbjct: 120 LSSTDYNGDRSYKSLQKFVSDSI-PSKVKILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 58.0 bits (134), Expect = 8e-10
Identities = 29/98 (29%), Positives = 52/98 (53%), Gaps = 1/98 (1%)
Frame = +3
Query: 219 ALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTCEQFSVSGYPTL 398
AL+ FYA WCGHCK L P Y G L D V + K+D + +++ ++G+PTL
Sbjct: 42 ALIEFYATWCGHCKSLAPVYE-ELGALFEDHNDVLIGKID-ADTHSDVADKYHITGFPTL 99
Query: 399 KIF-RKGELSSEYNGPRESNGIVKYMRAQVGPSSKELL 509
F G +Y+ R+ + + +++ + G ++++
Sbjct: 100 IWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIV 137
Score = 56.8 bits (131), Expect = 2e-09
Identities = 32/112 (28%), Positives = 53/112 (47%), Gaps = 3/112 (2%)
Frame = +3
Query: 162 DVLDLTDSDFS-AXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVD 338
+V++L +F + LV FYA WCG+CKRL P Y + K + P V + K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNE-PNVEIVKIN 199
Query: 339 CTEGGKSTCEQFSVSGYPTLKIFRKGELSSE--YNGPRESNGIVKYMRAQVG 488
+ V+ +PT+K F K + Y G R +++Y+ + G
Sbjct: 200 -ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 43.6 bits (98), Expect = 2e-05
Identities = 29/112 (25%), Positives = 53/112 (47%), Gaps = 4/112 (3%)
Frame = +3
Query: 174 LTDSDFSAXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDV--PPVALAKVDCTE 347
LTD+D + +S+ T + +Y P CG CKRL P + K V +VDC++
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 348 GGKSTCEQFSVSGYPTLKIFRKGELSSE--YNGPRESNGIVKYMRAQVGPSS 497
S+C ++ PTL +++ GE+ E + ++ ++ + P +
Sbjct: 90 -ELSSCA--NIRAVPTLYLYQNGEIVEEVPFGASTSEASLLDFVETHLNPDT 138
Score = 39.5 bits (88), Expect = 3e-04
Identities = 24/97 (24%), Positives = 44/97 (45%)
Frame = +3
Query: 180 DSDFSAXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKS 359
D+D A L+ + + FY+ C C + + A ++ + +A ++C K
Sbjct: 288 DADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLN---VAHINCAVS-KR 343
Query: 360 TCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 470
C+Q+S+ +PT +F K E EY G +V +
Sbjct: 344 ACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 37.9 bits (84), Expect = 0.001
Identities = 23/78 (29%), Positives = 35/78 (44%)
Frame = +3
Query: 186 DFSAXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTC 365
D++ +S +V FYA WCG CK LKP L + V+ +
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVN-ADKFSDIA 81
Query: 366 EQFSVSGYPTLKIFRKGE 419
++ V PT+ +FRKG+
Sbjct: 82 QKNGVYALPTMVLFRKGQ 99
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 37.5 bits (83), Expect = 0.001
Identities = 22/86 (25%), Positives = 36/86 (41%)
Frame = +3
Query: 183 SDFSAXLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKST 362
S+F + + Q +V F+A WCG CK + P++ KVD + +
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFE----QFSNTYSDATFIKVDVDQLSEIA 64
Query: 363 CEQFSVSGYPTLKIFRKGELSSEYNG 440
E V P+ +++ GE E G
Sbjct: 65 AEA-GVHAMPSFFLYKNGEKIEEIVG 89
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 35.5 bits (78), Expect = 0.005
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +3
Query: 234 YAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRK 413
YA WCG CK + P ++ A K P AKV+ E + V PT F
Sbjct: 27 YADWCGPCKAISPLFSQLAS--KYASPKFVFAKVNVDE-QRQIASGLGVKAMPTFVFFEN 83
Query: 414 GE 419
G+
Sbjct: 84 GK 85
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 27.9 bits (59), Expect = 1.0
Identities = 17/73 (23%), Positives = 29/73 (39%)
Frame = +3
Query: 222 LVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTCEQFSVSGYPTLK 401
L+ FYAPW CK++ + A D K++ E E F V+ P
Sbjct: 24 LLNFYAPWAAPCKQMNQVFDQFA----KDTKNAVFLKIE-AEKFSDIAESFDVNAVPLFV 78
Query: 402 IFRKGELSSEYNG 440
+ ++ + +G
Sbjct: 79 LIHGAKVLARISG 91
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.2 bits (55), Expect = 3.1
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1
Query: 166 FSILQIPTFRLXYLNMIQPWSCF 234
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPBP16F5.05c |||ribosome biogenesis protein
Nop8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 146
Score = 25.4 bits (53), Expect = 5.5
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 132 IIYLCKAAEEDVLDLTDSDFSAXLSQHD 215
+IY C+AA+E++LD LS+ D
Sbjct: 6 LIYACRAADEELLDEIIEKCPQELSRRD 33
>SPAC664.07c |rad9||checkpoint clamp complex protein
Rad9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 351 GKSTCEQFSVSGYPTLKIFRKGELS 425
G S C + V G P L F KG+ S
Sbjct: 265 GSSICAYYGVPGKPILLTFAKGKNS 289
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,065,441
Number of Sequences: 5004
Number of extensions: 39375
Number of successful extensions: 110
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -