BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10e08f (610 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 34 0.014 SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.8 SPBP23A10.09 |||GINS complex subunit Psf1 |Schizosaccharomyces p... 26 3.7 SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 5.0 SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch... 26 5.0 SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 25 8.7 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 25 8.7 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 25 8.7 SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 25 8.7 SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 25 8.7 >SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 451 Score = 34.3 bits (75), Expect = 0.014 Identities = 16/52 (30%), Positives = 27/52 (51%) Frame = +2 Query: 449 ILKLMEPCDHILEIQFPLRRDSGDYEMILGYRAQHSTHRTPTKGGIRFSTDV 604 +L ++ + +LE + D G+ + GYR Q ++ P KGG+RF V Sbjct: 35 VLPIISIPERVLEFRVTWEDDKGNCRVNTGYRVQFNSALGPYKGGLRFHPSV 86 >SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 575 Score = 26.6 bits (56), Expect = 2.8 Identities = 8/13 (61%), Positives = 12/13 (92%) Frame = +2 Query: 470 CDHILEIQFPLRR 508 CDHI+ +Q+PL+R Sbjct: 120 CDHIVNLQYPLKR 132 >SPBP23A10.09 |||GINS complex subunit Psf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 202 Score = 26.2 bits (55), Expect = 3.7 Identities = 24/87 (27%), Positives = 40/87 (45%), Gaps = 1/87 (1%) Frame = -3 Query: 545 RDSLISFRNRQNRASEEIESQECDRMVP*VLEYRLLSFSSLQWVSLTSNLQQACLRQLDR 366 R+SL +N + AS+ + Q+ +L + S + + + NL+ LRQ Sbjct: 45 RESLGILQNVTHEASQPFQPQDHPSEAAALLMFHSSSIYNKRCLMAYHNLRLQRLRQYCW 104 Query: 365 LGGKNILPCGRTS-DSHL*EYL*AYLE 288 GGK + C TS ++ +YL Y E Sbjct: 105 SGGKRMESCLDTSLSTYERDYLTRYSE 131 >SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 25.8 bits (54), Expect = 5.0 Identities = 22/76 (28%), Positives = 34/76 (44%) Frame = +2 Query: 206 QNEALNTMFRIIPAGVNVCCRTYASHEIPDKLKDIPTSANPKFFHMVEYFFHRACQVVED 385 QN+++N R+I ++ R Y IP +D A H E F+ R Q Sbjct: 667 QNKSMNERLRVIGKRIDHLERAYRREAIPLWEEDAKQQAE----HDREIFYEREKQ---R 719 Query: 386 KLVEDLKSRTPIEEKK 433 K V++ K I++KK Sbjct: 720 KEVQERKHEQAIKDKK 735 >SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosaccharomyces pombe|chr 1|||Manual Length = 558 Score = 25.8 bits (54), Expect = 5.0 Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 1/59 (1%) Frame = -3 Query: 548 VRDSLISFRNRQNRASEEIES-QECDRMVP*VLEYRLLSFSSLQWVSLTSNLQQACLRQ 375 ++D + + + + + EIES + + + EYR LS + + VSL S + Q R+ Sbjct: 287 IKDYVSHLQTKSTKKASEIESIADMKQFLEAYPEYRRLSGNVSKHVSLVSEISQVVQRE 345 >SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1469 Score = 25.0 bits (52), Expect = 8.7 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -1 Query: 286 LMTSVCAAADIHSSWYDSEHCIEGFIL 206 LM V +A DIH+S + +HC + ++ Sbjct: 762 LMDDVFSALDIHTSNWIYKHCFQSSLM 788 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 25.0 bits (52), Expect = 8.7 Identities = 8/27 (29%), Positives = 14/27 (51%) Frame = +1 Query: 313 YKCESEVLPHGRIFFPPSLSSCRRQAC 393 Y CE ++ PP ++CR++ C Sbjct: 89 YNCEKQICRKCYARLPPRATNCRKKKC 115 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 25.0 bits (52), Expect = 8.7 Identities = 8/27 (29%), Positives = 14/27 (51%) Frame = +1 Query: 313 YKCESEVLPHGRIFFPPSLSSCRRQAC 393 Y CE ++ PP ++CR++ C Sbjct: 89 YNCEKQICRKCYARLPPRATNCRKKKC 115 >SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 682 Score = 25.0 bits (52), Expect = 8.7 Identities = 10/29 (34%), Positives = 18/29 (62%), Gaps = 1/29 (3%) Frame = -3 Query: 572 LESCVWNVVRDSLISFRNRQN-RASEEIE 489 + C+WN++R NR+N RA+ E++ Sbjct: 612 MRRCMWNILRVEHEEIYNRENLRAARELK 640 >SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 4196 Score = 25.0 bits (52), Expect = 8.7 Identities = 9/34 (26%), Positives = 21/34 (61%) Frame = +2 Query: 341 MVEYFFHRACQVVEDKLVEDLKSRTPIEEKKKKV 442 +++ ++H AC+V+ D+LV + + E +K + Sbjct: 2744 LIKVWYHEACRVLLDRLVSQKECSWGMTELQKVI 2777 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,605,269 Number of Sequences: 5004 Number of extensions: 54288 Number of successful extensions: 144 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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