Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10e04f
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 183 2e-47
SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 97 3e-21
SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 59 5e-10
SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharo... 35 0.012
SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 32 0.083
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 29 0.59
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 4.1
SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex... 26 4.1
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 5.5
SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch... 26 5.5
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 26 5.5
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 5.5
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 7.2
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 7.2
>SPAC23H4.09 |cdb4||curved DNA-binding protein
Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 381
Score = 183 bits (446), Expect = 2e-47
Identities = 93/209 (44%), Positives = 129/209 (61%), Gaps = 2/209 (0%)
Frame = +2
Query: 32 EMADEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEET 211
E E V+ +++ V KYK+AG++ V+++V+ C P A +IC GD+L+ E
Sbjct: 5 EATSETAVDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYDICVRGDELLNEAI 64
Query: 212 NKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIASEPD--YILKKGDLAKIDLGAHIDGFI 385
KV++ KD+ KGIAF T VS N+ H SP+ S+P+ LK GD+ KI LGAHIDGF
Sbjct: 65 KKVYRT-KDAYKGIAFPTAVSPNDMAAHLSPLKSDPEANLALKSGDVVKILLGAHIDGFA 123
Query: 386 AVVAHTVVVGESEVSGRAADVXXXXXXXXXXXXXXXKPGTENYAVTEAIQKISAEYGCKP 565
++VA T VV E V+G AADV KPG N+ VT+ + KI+ YGCKP
Sbjct: 124 SLVATTTVVSEEPVTGPAADVIAAASAALKAAQRTIKPGNTNWQVTDIVDKIATSYGCKP 183
Query: 566 IEGMLSHQLKQFRIDGEKSIIQNPSEAQR 652
+ GMLSHQ ++ IDG+K +I NPS++QR
Sbjct: 184 VAGMLSHQQEREVIDGKKQVILNPSDSQR 212
>SPBC23E6.05 |arx1||ribosomal export complex Arx1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 417
Score = 96.7 bits (230), Expect = 3e-21
Identities = 46/130 (35%), Positives = 78/130 (60%), Gaps = 6/130 (4%)
Frame = +2
Query: 41 DEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKV 220
D + + + +KY+ AG +V++ QV ++CVP AS REI +GD L+ E + +
Sbjct: 4 DPSDSNSRVVDASQFSKYRDAGALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSI 63
Query: 221 FKKEKDSKKGIAFSTCVSVNNCICHFSP------IASEPDYILKKGDLAKIDLGAHIDGF 382
+K ++ +KGIA T + VNNC +++P ++ Y L+ GD+ KI +G H DG+
Sbjct: 64 YKSQR-FEKGIAEPTSICVNNCAYNYAPGPESVIAGNDNSYHLQVGDVTKISMGLHFDGY 122
Query: 383 IAVVAHTVVV 412
A+++HT+VV
Sbjct: 123 TALISHTIVV 132
>SPBC14C8.03 |fma2||methionine aminopeptidase Fma2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 59.3 bits (137), Expect = 5e-10
Identities = 40/147 (27%), Positives = 65/147 (44%), Gaps = 5/147 (3%)
Frame = +2
Query: 200 LEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIASEPDYILKKGDLAKIDLGAHIDG 379
+E T + +E K GI F T VS+N+C H++P A + ILK+ D+ K+D+G H++G
Sbjct: 148 IENTTRALVEEDGLKSGIGFPTGVSLNHCAAHYTPNAGDTT-ILKEKDVMKVDIGVHVNG 206
Query: 380 FIAVVAHTVVVGESEVSGRAADVXXXXXXXXXXXXXXXKPGTENYAVTEAIQKISAE--- 550
I A T+ + + A V + A+ E ++ E
Sbjct: 207 RIVDSAFTMSF-DPQYDNLLAAVKAATNKGIEEAGIDARLNEIGEAIQEVMESYEVEING 265
Query: 551 --YGCKPIEGMLSHQLKQFRIDGEKSI 625
+ K I + H L + I G KS+
Sbjct: 266 KTHQVKSIRNLCGHNLDPYIIHGGKSV 292
>SPBC3E7.10 |fma1||methionine aminopeptidase Fma1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 379
Score = 34.7 bits (76), Expect = 0.012
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +2
Query: 260 STCVSVNNCICHFSPIASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGE 418
S C SVN ICH P P L+ GD+ ID+ + +GF + T VG+
Sbjct: 181 SVCTSVNEIICHGIP-DQRP---LEDGDIVNIDVSLYHNGFHGDLNETYYVGD 229
>SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 117
Score = 31.9 bits (69), Expect = 0.083
Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 3/71 (4%)
Frame = -3
Query: 565 WLTTIFSTYFLNGFSDCIVLSTR---LQETKSRFTSMMSCQQNICSTSRDFTFTHNYCMS 395
W++++ L G S I LST L + K+ + M + C S +T + N C +
Sbjct: 5 WVSSLLG---LLGLSTAIPLSTEADALLDRKTIYFGMKAYDDVNCKGSTSYTISFNKCQA 61
Query: 394 YNSYKSINVST 362
Y + SIN T
Sbjct: 62 YPNINSINALT 72
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 29.1 bits (62), Expect = 0.59
Identities = 11/30 (36%), Positives = 22/30 (73%)
Frame = -2
Query: 344 NLLFSECNPVRSQWAKSDKCSCSQKRMLKM 255
+L+ S+C V+ QW+ ++CSCS + +L++
Sbjct: 2135 DLICSKCKTVK-QWSLKERCSCSGEWVLQL 2163
>SPAC343.11c |msc1||multi-copy suppressor of Chk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1588
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +3
Query: 147 AFQTHQQEKYVNLAINLYWRRLTRFSKRRKTP 242
AF T +Q Y N NLY+ ++ S R TP
Sbjct: 474 AFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTP 505
>SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex
subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 95
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/40 (30%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = +2
Query: 41 DEKEV-EKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPD 157
D+K + K I ++L V + AG++++++ E KC+P+
Sbjct: 16 DKKSIFMKQIRQELAVAQ---AGELISKINENCFDKCIPE 52
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.8 bits (54), Expect = 5.5
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = -3
Query: 406 YCMSYNSYKSINVSTKIYLG*ISF 335
YC +YKS+ + T +Y+G F
Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167
>SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 658
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = -2
Query: 572 PQLAYNHIQHLFSE---WLQ*LHSSQYQASGDEEPLH*HDELPTKHLQHVQRLHFHPQLL 402
P LAY + + FS + +SS + +P ++ L ++ LQ +LH+H +L
Sbjct: 375 PSLAYLQVVYSFSMRALLVNRQYSSAFAFFWKIDPYIRNERLVSQMLQAASQLHYHDLIL 434
Query: 401 Y 399
Y
Sbjct: 435 Y 435
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 25.8 bits (54), Expect = 5.5
Identities = 9/34 (26%), Positives = 17/34 (50%)
Frame = -3
Query: 637 WILYNTLLSINTELLQLV*EHTLNWLTTIFSTYF 536
W++Y+ L+ + E + V WL + T+F
Sbjct: 1095 WVIYHELIQTSKEYMHTVSTVNPKWLVEVAPTFF 1128
>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 681
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -3
Query: 196 KFIAKFTYFSC*CVWNAFCDDLLKDSVHYLSC*LV 92
K + KF+ C +++A C+D L S H C +V
Sbjct: 472 KCLNKFSQEDCQFIFDAICEDPLDVSTHRHGCCVV 506
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 158 ASAREICEFGDKLVLEETNKVFKKEKD 238
++ +E FG+KL E T K+ EKD
Sbjct: 865 SNLQEKINFGNKLAKEHTEKISSLEKD 891
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +2
Query: 314 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 409
EP+Y+L G+L+ +L ID F T V
Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,603,795
Number of Sequences: 5004
Number of extensions: 52317
Number of successful extensions: 179
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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