BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10d22f (601 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23C4.07 |tht2|mug22|meiotically upregulated gene Mug22|Schiz... 29 0.69 SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr ... 28 1.2 SPAC1071.01c |pta1|SPAC4H3.15c|mRNA cleavage and polyadenylation... 28 1.2 SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 25 6.4 SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 25 8.5 SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch... 25 8.5 >SPAC23C4.07 |tht2|mug22|meiotically upregulated gene Mug22|Schizosaccharomyces pombe|chr 1|||Manual Length = 201 Score = 28.7 bits (61), Expect = 0.69 Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 3/63 (4%) Frame = +2 Query: 29 YSDRANVKIYLISTRNRVFFLFVNTLEQVEQNTDFIAQTFS---AGFEMTCNIDQVVPAT 199 Y +++ +K +ST N V +F++ +Q+EQ F +S FE T + + Sbjct: 80 YKNQSLMKKTSVSTSNTVKMVFMSLAKQIEQMLKFCMMVYSKLCEAFETTLKVAKEFQIC 139 Query: 200 DAS 208 D+S Sbjct: 140 DSS 142 >SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 774 Score = 27.9 bits (59), Expect = 1.2 Identities = 19/74 (25%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Frame = +2 Query: 59 LISTRNRVFFLFVNTLEQVEQNTDFIAQTFSAGFEMTCNIDQVVPATD--ASGVIMNGIT 232 ++++ ++ FF+F N E N+D + Q F G E+ VP + + ++ GI Sbjct: 332 MLASLSKAFFIFDNQNEIFNFNSDSLQQQFRQGEEIVKQSFFQVPDYNILVATLLREGIE 391 Query: 233 EVRGHF-IRDNVPV 271 ++ + IR +PV Sbjct: 392 NLKDNMSIRPGIPV 405 >SPAC1071.01c |pta1|SPAC4H3.15c|mRNA cleavage and polyadenylation specificity factor complex subunit Pta1|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 27.9 bits (59), Expect = 1.2 Identities = 24/94 (25%), Positives = 43/94 (45%), Gaps = 5/94 (5%) Frame = +2 Query: 92 FVNT-LEQVEQNTDFIAQTFSAGFEMTCNIDQVVPATDASGVIMNGITEVR----GHFIR 256 F+N+ + ++E N D I + FS F + N+ + + V++ E+ G + Sbjct: 180 FINSNILRIEAN-DLIEKIFSILFSDSLNVLYITSVLNILPVLVKRRKELAPKIIGSLLE 238 Query: 257 DNVPVPADEIETLRGDMVLLTAIQSTLATRLLVL 358 ++P P DEIE + + I+ L LL L Sbjct: 239 FHLPNPKDEIELSNESKLAIRCIEKNLKLILLHL 272 >SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 697 Score = 25.4 bits (53), Expect = 6.4 Identities = 11/37 (29%), Positives = 22/37 (59%), Gaps = 3/37 (8%) Frame = +2 Query: 128 DFIAQTFSA---GFEMTCNIDQVVPATDASGVIMNGI 229 +FIA T + GF C+++ ++PA ++S + G+ Sbjct: 617 EFIAVTDAQCLEGFRALCHLEGIIPALESSHAVYGGM 653 >SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 25.0 bits (52), Expect = 8.5 Identities = 7/10 (70%), Positives = 8/10 (80%) Frame = -3 Query: 596 HVYWCPAPRC 567 H+ WCPAP C Sbjct: 213 HLRWCPAPDC 222 >SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 25.0 bits (52), Expect = 8.5 Identities = 8/23 (34%), Positives = 16/23 (69%) Frame = +2 Query: 182 QVVPATDASGVIMNGITEVRGHF 250 + + T+ +G++ G+T+V GHF Sbjct: 306 EAITRTNRAGLLPVGVTKVHGHF 328 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,031,572 Number of Sequences: 5004 Number of extensions: 34064 Number of successful extensions: 93 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 91 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 93 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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