BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10d17r (381 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosacc... 85 3e-18 SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Sch... 85 4e-18 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 27 0.75 SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb... 27 0.99 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 26 1.7 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 26 2.3 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 3.0 SPAC4F10.06 |||BUD22 family protein|Schizosaccharomyces pombe|ch... 25 3.0 SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 25 4.0 SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 25 4.0 SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc... 25 4.0 SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 25 4.0 SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 24 9.3 SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 24 9.3 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 24 9.3 >SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosaccharomyces pombe|chr 3|||Manual Length = 99 Score = 85.4 bits (202), Expect = 3e-18 Identities = 44/86 (51%), Positives = 59/86 (68%) Frame = -2 Query: 275 KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRALKFLK 96 K +T + + RP+R KG +K + FVR +VREV G A YE+R MEL++ S+DKRA K K Sbjct: 13 KVLTKRQLPERPSRRKGQLSKRTSFVRSIVREVAGFAPYERRVMELIRNSQDKRARKLAK 72 Query: 95 RRLGTHIRAKRKREELSNVLAQMRKA 18 +RLGT RAK K EEL++V+ R A Sbjct: 73 KRLGTLKRAKGKIEELTSVIQSSRLA 98 >SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Schizosaccharomyces pombe|chr 2|||Manual Length = 99 Score = 85.0 bits (201), Expect = 4e-18 Identities = 44/86 (51%), Positives = 59/86 (68%) Frame = -2 Query: 275 KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRALKFLK 96 K +T + + RP+R KG +K + FVR +VREV G A YE+R MEL++ S+DKRA K K Sbjct: 13 KTLTKRQLPERPSRRKGHLSKRTAFVRSIVREVAGFAPYERRVMELIRNSQDKRARKLAK 72 Query: 95 RRLGTHIRAKRKREELSNVLAQMRKA 18 +RLGT RAK K EEL++V+ R A Sbjct: 73 KRLGTLKRAKGKIEELTSVIQSSRLA 98 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 27.5 bits (58), Expect = 0.75 Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 1/53 (1%) Frame = -2 Query: 179 VVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAK-RKREELSNVLAQMR 24 ++ HA ++ L+VSKD KRR+ THI + +R LS V Q+R Sbjct: 1119 LINHADILSSILDYLQVSKD-------KRRMATHILGQINQRLTLSQVRNQLR 1164 >SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 27.1 bits (57), Expect = 0.99 Identities = 14/37 (37%), Positives = 26/37 (70%), Gaps = 3/37 (8%) Frame = -1 Query: 189 STRSCRTRSI*EEGY---GVT*GVKRQACSEVLEATI 88 +TR+C T+S+ EEG+ V+ +KR+ +++L+A I Sbjct: 928 ATRACLTQSLEEEGFPSRNVSNEIKRRFLTDLLKARI 964 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 26.2 bits (55), Expect = 1.7 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = +3 Query: 6 SLGGCLPHLSEHVAKFFTLPLGADVCAQSSLQELQSTLV 122 SL G L + A+FFT PL ++VCA + + + ++ Sbjct: 547 SLNGEYIALEKIEAQFFTSPLVSNVCAYADVNHAKPVVI 585 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 25.8 bits (54), Expect = 2.3 Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 4/48 (8%) Frame = -2 Query: 191 LVREVVGHAQYEKRAMELLKVSKDKRALKFLKRRL----GTHIRAKRK 60 ++RE +G + K + ++ K ALKF+ R+L H+R +R+ Sbjct: 35 IIRETLGEGSFGKVKLATHYKTQQKVALKFISRQLLKKSDMHMRVERE 82 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 25.4 bits (53), Expect = 3.0 Identities = 13/39 (33%), Positives = 27/39 (69%) Frame = -2 Query: 143 ELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLAQM 27 +LLK ++ + K K+RL +I K K+EE+++++A++ Sbjct: 75 QLLKENEGSISSK-KKKRLDKYIENKLKKEEVASLIAKL 112 >SPAC4F10.06 |||BUD22 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 25.4 bits (53), Expect = 3.0 Identities = 15/61 (24%), Positives = 30/61 (49%) Frame = -2 Query: 185 REVVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLAQMRKAAAQA 6 R+ + +Y K A L+K + ++R+++ ++R +AKR E + Q + Q Sbjct: 303 RQAIWEKKYGKGANHLIKKATEERSIREERQRKYEERQAKRAARENAFTEHQTPQKPEQL 362 Query: 5 H 3 H Sbjct: 363 H 363 >SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 25.0 bits (52), Expect = 4.0 Identities = 22/102 (21%), Positives = 46/102 (45%), Gaps = 2/102 (1%) Frame = -2 Query: 302 KTTKISAGRKGITDKAIRIR--PARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKV 129 K ++ ++ + + +R++ P L+ Q S+ R+L ++ Q EK+ EL++ Sbjct: 424 KQNVVNEAKRYLQETILRLQAIPYHLQVGQAWTSESERELEKK---KEQVEKKQEELMQT 480 Query: 128 SKDKRALKFLKRRLGTHIRAKRKREELSNVLAQMRKAAAQAH 3 FL + +AKRK E+ +L+ + + H Sbjct: 481 RIVLEEQVFLVENMIEDAKAKRKFSEVETLLSSLAPLHEEIH 522 >SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 25.0 bits (52), Expect = 4.0 Identities = 8/22 (36%), Positives = 17/22 (77%) Frame = +1 Query: 118 LSFDTLSNSIALFSY*ACPTTS 183 +S+D+LS+ +++ S+ CP T+ Sbjct: 79 MSYDSLSDDVSVLSFLDCPLTT 100 >SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly scaffold protein Isu1|Schizosaccharomyces pombe|chr 1|||Manual Length = 192 Score = 25.0 bits (52), Expect = 4.0 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = -2 Query: 245 RPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAME 141 RPA L+ L+T SKFV + V Y K ++ Sbjct: 27 RPANLQFLKTNSSKFVPQVTAN-VSRRMYHKNVLD 60 >SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 941 Score = 25.0 bits (52), Expect = 4.0 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = +1 Query: 130 TLSNSIALFSY*ACPTTSRTKSRTNLECF 216 T+++S LF+ A T+ TKS N+EC+ Sbjct: 850 TINHSRTLFATHAHQLTNLTKSFKNVECY 878 >SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|Schizosaccharomyces pombe|chr 3|||Manual Length = 551 Score = 23.8 bits (49), Expect = 9.3 Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 1/54 (1%) Frame = +3 Query: 9 LGGCLPHLSEHVAKFFTLPLGA-DVCAQSSLQELQSTLVF*HLK*LHSPLLILS 167 +GG + SEHV K F L V ++ ++ S + LK + S LL ++ Sbjct: 127 IGGLIAEQSEHVEKAFNLLRQVLSVALKAQKPDMDSPELLEFLKPIQSELLTIT 180 >SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 490 Score = 23.8 bits (49), Expect = 9.3 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +2 Query: 74 GCVCPIVASRTSEHACLLT 130 GCVCP++ SE A +L+ Sbjct: 189 GCVCPLLIDLESEDADVLS 207 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 23.8 bits (49), Expect = 9.3 Identities = 21/63 (33%), Positives = 31/63 (49%), Gaps = 7/63 (11%) Frame = -2 Query: 308 GHKTTKISAGRKGITDKAIRIRPARLKGL------QTKHSKFV-RDLVREVVGHAQYEKR 150 G+ K S TDK I+P LKGL TK ++ + RDL+R V+G E + Sbjct: 730 GNPVDKNSTSPSKSTDKLDPIKPVFLKGLFSVSTTSTKSTESIQRDLIR-VMGMLDIEYK 788 Query: 149 AME 141 ++ Sbjct: 789 EIK 791 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,513,846 Number of Sequences: 5004 Number of extensions: 26986 Number of successful extensions: 77 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 77 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 77 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 124270298 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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