SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10d15r
         (675 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34...    57   1e-08
At5g15090.1 68418.m01768 porin, putative / voltage-dependent ani...    56   3e-08
At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34...    42   3e-04
At3g20000.1 68416.m02530 porin family protein low similarity to ...    36   0.019
At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34...    35   0.043
At5g37610.1 68418.m04530 hypothetical protein                          33   0.23 
At2g33860.1 68415.m04157 auxin-responsive factor (ARF3) / ETTIN ...    28   5.0  
At2g30575.1 68415.m03725 glycosyl transferase family 8 protein c...    28   5.0  
At2g27770.1 68415.m03365 expressed protein                             28   5.0  
At1g05080.1 68414.m00510 F-box family protein contains F-box dom...    28   5.0  
At3g04460.1 68416.m00473 Pex2/Pex12 N-terminal domain-containing...    27   8.7  

>At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34
           kDa outer mitochondrial membrane protein porin
           (Voltage-dependent anion-selective channel protein)
           (VDAC) {Solanum tuberosum}; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 276

 Score = 56.8 bits (131), Expect = 1e-08
 Identities = 37/135 (27%), Positives = 61/135 (45%), Gaps = 3/135 (2%)
 Frame = -3

Query: 667 GVHTQFDTQKAKFSKNNFALGYQSGDFALHTNV-DNGKDFGGSIYQKVSDKLD--CGVSM 497
           G    FDT+   F+K N  L +   D      V D G     S Y  V+   +   G  +
Sbjct: 143 GTDVSFDTKSGNFTKINAGLSFTKEDLIASLTVNDKGDLLNASYYHIVNPLFNTAVGAEV 202

Query: 496 KWTAGSADTLFGVGAKYALDQDASLHAKINNKSLIGLGYQQKLRPGVTLTLSAAIDGQNF 317
                S D+   VG +++LD   S+ A++N+  +     Q + +P    T+S  +D ++ 
Sbjct: 203 SHKLSSKDSTITVGTQHSLDPLTSVKARVNSAGIASALIQHEWKPKSFFTISGEVDTKSI 262

Query: 316 NAGGHKVGVALELEP 272
           +    KVG+AL L+P
Sbjct: 263 DKSA-KVGLALALKP 276


>At5g15090.1 68418.m01768 porin, putative / voltage-dependent
           anion-selective channel protein, putative similar to
           SP|P42055 34 kDa outer mitochondrial membrane protein
           porin (Voltage-dependent anion-selective channel
           protein) (VDAC) {Solanum tuberosum}; contains Pfam
           profile PF01459: Eukaryotic porin; identical to cDNA
           voltage-dependent anion-selective channel protein
           GI:4006940
          Length = 274

 Score = 55.6 bits (128), Expect = 3e-08
 Identities = 34/133 (25%), Positives = 57/133 (42%), Gaps = 1/133 (0%)
 Frame = -3

Query: 667 GVHTQFDTQKAKFSKNNFALGYQSGDFALHTNV-DNGKDFGGSIYQKVSDKLDCGVSMKW 491
           G    ++T+   F   N    +   D      + D G+    S YQ VS     G  +  
Sbjct: 143 GTDVAYNTESGNFKHFNAGFNFTKDDLTASLILNDKGEKLNASYYQIVSPSTVVGAEISH 202

Query: 490 TAGSADTLFGVGAKYALDQDASLHAKINNKSLIGLGYQQKLRPGVTLTLSAAIDGQNFNA 311
              + +    VG ++ALD   ++ A++NN  +     Q + RP    T+S  +D +  + 
Sbjct: 203 NFTTKENAITVGTQHALDPLTTVKARVNNAGVANALIQHEWRPKSFFTVSGEVDSKAIDK 262

Query: 310 GGHKVGVALELEP 272
              KVG+AL L+P
Sbjct: 263 SA-KVGIALALKP 274


>At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34
           kDa outer mitochondrial membrane protein porin
           (Voltage-dependent anion-selective channel protein)
           (VDAC) {Solanum tuberosum}; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 276

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 34/135 (25%), Positives = 56/135 (41%), Gaps = 3/135 (2%)
 Frame = -3

Query: 667 GVHTQFDTQKAKFSKNNFALGYQSGDFALHTNV-DNGKDFGGSIYQKVSD-KLDCGVSMK 494
           G    +DT    F+K N  +     D  L   + D G     S      + K    V   
Sbjct: 143 GAEAGYDTTSKTFTKYNAGISVTKPDACLSIILGDKGDSLKASYLHHFDEFKRTAAVGEV 202

Query: 493 WTAGSA-DTLFGVGAKYALDQDASLHAKINNKSLIGLGYQQKLRPGVTLTLSAAIDGQNF 317
           +   S  +    VG  YA+D   ++ AK+NN   +G   Q ++ P   +T+S+ ID +  
Sbjct: 203 YRKFSTNENTITVGGLYAIDHSTAVKAKLNNHGTLGALLQHEVLPRSLVTVSSEIDTKAL 262

Query: 316 NAGGHKVGVALELEP 272
                + G++L L+P
Sbjct: 263 EK-HPRFGLSLALKP 276


>At3g20000.1 68416.m02530 porin family protein low similarity to
           haymaker protein [Mus musculus] GI:17834089,
           mitochondrial outer membrane protein MOM35 [Mus
           musculus] GI:6650562; contains Pfam profile PF01459:
           Eukaryotic porin
          Length = 309

 Score = 36.3 bits (80), Expect = 0.019
 Identities = 21/69 (30%), Positives = 33/69 (47%)
 Frame = -3

Query: 535 QKVSDKLDCGVSMKWTAGSADTLFGVGAKYALDQDASLHAKINNKSLIGLGYQQKLRPGV 356
           QK+SDK+       +   S D    VG  Y L Q A +  KI++  +     +++L  G+
Sbjct: 227 QKISDKVSLATDFMYNYFSRDVTASVGYDYMLRQ-ARVRGKIDSNGVASALLEERLSMGL 285

Query: 355 TLTLSAAID 329
              LSA +D
Sbjct: 286 NFLLSAELD 294


>At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34
           kDa outer mitochondrial membrane protein porin
           (Voltage-dependent anion-selective channel protein)
           (VDAC) {Solanum tuberosum}; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 226

 Score = 35.1 bits (77), Expect = 0.043
 Identities = 32/132 (24%), Positives = 56/132 (42%), Gaps = 3/132 (2%)
 Frame = -3

Query: 658 TQFDTQKAKFSKNNFALGYQSGDFALHTNV-DNGKDFGGSI--YQKVSDKLDCGVSMKWT 488
           T +DT    F+K N  +     D  +   + D G     S   Y   S +      +   
Sbjct: 96  TGYDTTSRTFTKYNVGVSVTKPDQCVSIILGDKGDSIKASYVYYLDESTRSATVGEVIRK 155

Query: 487 AGSADTLFGVGAKYALDQDASLHAKINNKSLIGLGYQQKLRPGVTLTLSAAIDGQNFNAG 308
             + +T   VG  YA+D   ++ AK+N+    G   Q +  P   +T+S  ID +  +  
Sbjct: 156 ISTNETTVTVGGLYAVDHLTNVKAKLNSNGKFGALLQHEGLPKSIVTISGEIDTKTLDK- 214

Query: 307 GHKVGVALELEP 272
             ++G++L L+P
Sbjct: 215 YPRLGLSLSLKP 226


>At5g37610.1 68418.m04530 hypothetical protein
          Length = 163

 Score = 32.7 bits (71), Expect = 0.23
 Identities = 29/132 (21%), Positives = 49/132 (37%), Gaps = 2/132 (1%)
 Frame = -3

Query: 667 GVHTQFDTQKAKFSKNNFALGYQSGDFALHTNVDNGKD-FGGSIYQKVSDKLDCGVSMKW 491
           G     D      +K N  L   + D     ++D     F  S YQ++  K  CG   K+
Sbjct: 34  GFELTLDLVNRIITKANTVLSLITEDTTTTFSIDKKASLFTASYYQRLHSKTVCGAEAKY 93

Query: 490 TAGSADTLFGVGAKYALDQDASLHAKINNKSLIGLGYQQKLRPGVTLTLSAAIDGQN-FN 314
                   F +G ++    D  + A ++  +  G   +   +   TL     I G+   N
Sbjct: 94  ILSDKSNSFSIGKRHLFGDDDLIQALLSVSN--GGSMRVLFQHHWTLKSFFTIAGERLLN 151

Query: 313 AGGHKVGVALEL 278
               + G+A+EL
Sbjct: 152 HSSQRFGLAVEL 163


>At2g33860.1 68415.m04157 auxin-responsive factor (ARF3) / ETTIN
           protein (ETT) identical to ETTIN GB:AF007788 from
           [Arabidopsis thaliana]
          Length = 608

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 12/21 (57%), Positives = 16/21 (76%)
 Frame = -1

Query: 432 TRLCTPRSTTSPSSVLVTNRN 370
           TR+  P  ++SPSSVL+TN N
Sbjct: 514 TRILPPSVSSSPSSVLLTNSN 534


>At2g30575.1 68415.m03725 glycosyl transferase family 8 protein
           contains Pfam profile: PF01501 glycosyl transferase
           family 8
          Length = 610

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 12/37 (32%), Positives = 21/37 (56%)
 Frame = -2

Query: 284 RTRALENITKPTLVDKYILLSQPNSVYRESISIVEIT 174
           RT+ LE  T  T  DKY+  S PN +    +++ +++
Sbjct: 207 RTKELERATGDTTKDKYLPKSSPNRLKAMEVALYKVS 243


>At2g27770.1 68415.m03365 expressed protein
          Length = 320

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 12/37 (32%), Positives = 21/37 (56%)
 Frame = -1

Query: 138 LSCFPKNAVRLFH*LFLNVCKQFASKYSLTRLLINKS 28
           +SCF +N++ + H L ++      SKYS   + I+ S
Sbjct: 5   VSCFSENSINVTHPLSISSSSSSCSKYSTNNVCISPS 41


>At1g05080.1 68414.m00510 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 439

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 16/48 (33%), Positives = 32/48 (66%)
 Frame = -2

Query: 266 NITKPTLVDKYILLSQPNSVYRESISIVEITCIVPGAFKTQYSFPAFL 123
           ++++ TL D+ IL++ P+S Y  S++ +E+ C+V   +K + S  +FL
Sbjct: 141 SLSELTLSDQ-ILVNVPSSAYLPSLTELELICVV---YKDEDSLVSFL 184


>At3g04460.1 68416.m00473 Pex2/Pex12 N-terminal domain-containing
           protein similar to SP|O00623 Peroxisome assembly protein
           12 (Peroxin-12) (Peroxisome assembly factor-3) (PAF-3)
           {Homo sapiens}; contains Pfam profile PF04757: Pex2 /
           Pex12 amino terminal region
          Length = 393

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
 Frame = +3

Query: 234 VFIYKSRFGYIF*GSSSRATPTLWPPALKF-CPSMAADNVRVTPGRSFC 377
           VFI+K    +        + PT++PP      P MA + + + P RS C
Sbjct: 291 VFIFKMMEWWYQSAEERLSAPTVYPPPPPPPAPKMAKEGIPLPPDRSLC 339


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,452,975
Number of Sequences: 28952
Number of extensions: 343870
Number of successful extensions: 878
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 848
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 878
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -