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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10d04r
         (731 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot...   254   4e-68
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ...   254   6e-68
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S...   195   2e-50
At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma...    30   1.4  
At5g16290.2 68418.m01904 acetolactate synthase small subunit, pu...    29   4.2  
At5g16290.1 68418.m01903 acetolactate synthase small subunit, pu...    29   4.2  
At4g37290.1 68417.m05277 expressed protein                             29   4.2  
At3g59870.1 68416.m06681 expressed protein hypothetical protein ...    29   4.2  
At4g27060.1 68417.m03891 expressed protein                             27   9.7  

>At1g74030.1 68414.m08573 enolase, putative similar to
           Swiss-Prot:P15007 enolase (EC 4.2.1.11)
           (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
           hydro-lyase) [Drosophila melanogaster]
          Length = 477

 Score =  254 bits (622), Expect = 4e-68
 Identities = 120/171 (70%), Positives = 142/171 (83%)
 Frame = -2

Query: 703 KNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGRTPIQIVGDDL 524
           K   ++    LS++ LAD+Y +FIKDFP+VSIEDPFDQDDWS+WA+L     IQ+VGDDL
Sbjct: 308 KKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDL 367

Query: 523 TVTNPKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETEDTF 344
            VTNPKRIA A++K++CN LLLKVNQIG+VTESI A L +K  GWG MVSHRSGETED F
Sbjct: 368 LVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNF 427

Query: 343 IADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGVNAKYAGKNFRRP 191
           IADL VGL++GQIKTGAPCRSERL+KYNQ+LRIEEELG N +YAG+ FR P
Sbjct: 428 IADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELG-NVRYAGEAFRSP 477


>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
           enolase (EC 4.2.1.11) (2-phosphoglycerate
           dehydratase)(2-phospho-D- glycerate hydro-lyase)
           [Arabidopsis thaliana]
          Length = 444

 Score =  254 bits (621), Expect = 6e-68
 Identities = 120/174 (68%), Positives = 140/174 (80%), Gaps = 2/174 (1%)
 Frame = -2

Query: 703 KNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGR--TPIQIVGD 530
           K  ++N    +S D L D+Y  F+ ++P+VSIEDPFDQDDW  +A +T    T +QIVGD
Sbjct: 268 KEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQDDWEHYAKMTTECGTEVQIVGD 327

Query: 529 DLTVTNPKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETED 350
           DL VTNPKR+A A+ +K+CN LLLKVNQIGSVTESI+A  ++KK GWG M SHRSGETED
Sbjct: 328 DLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEAVKMSKKAGWGVMTSHRSGETED 387

Query: 349 TFIADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGVNAKYAGKNFRRPV 188
           TFIADL VGLSTGQIKTGAPCRSERLAKYNQ+LRIEEELG  A YAG NFR+PV
Sbjct: 388 TFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEELGSEAIYAGVNFRKPV 441


>At2g29560.1 68415.m03590 enolase, putative similar to enolase
           [Spinacia oleracea] gi|8919731|emb|CAB96173
          Length = 475

 Score =  195 bits (476), Expect = 2e-50
 Identities = 87/170 (51%), Positives = 125/170 (73%)
 Frame = -2

Query: 706 LKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGRTPIQIVGDD 527
           +K+P+ +  ++ S++ + D+Y +   D+P+VSIEDPFD++DW      +     Q+VGDD
Sbjct: 303 IKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVGDD 362

Query: 526 LTVTNPKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETEDT 347
           L ++N KR+  A+++ +CN LLLKVNQIG+VTE+I+   +A+   WG + SHR GETED+
Sbjct: 363 LLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETEDS 422

Query: 346 FIADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGVNAKYAGKNFR 197
           FI+DL VGL+TG IK GAPCR ER  KYNQ+LRIEEELG  A YAG++++
Sbjct: 423 FISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472


>At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain,
           PF04937: Protein of unknown function (DUF 659)
          Length = 433

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 19/40 (47%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
 Frame = +3

Query: 219 LALTPSSSSMRRIWLYLARRSER----HGAPVLI*PVDKP 326
           L+LTPSSSS  R W      SER    +G PV   P +KP
Sbjct: 358 LSLTPSSSSFERNWSAFEGPSERDEEPNGEPVYQTPNEKP 397


>At5g16290.2 68418.m01904 acetolactate synthase small subunit,
           putative similar to gi:5931761 from Nicotiana
           plumbaginifolia
          Length = 477

 Score = 28.7 bits (61), Expect = 4.2
 Identities = 15/53 (28%), Positives = 26/53 (49%)
 Frame = +2

Query: 479 LLLNCSSDTLRVCHR*IITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 637
           L+   S +T+    +  +T N N  +  +V P  P    K +L+ H G ++DE
Sbjct: 248 LVKESSHETVAEKTKLALTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300


>At5g16290.1 68418.m01903 acetolactate synthase small subunit,
           putative similar to gi:5931761 from Nicotiana
           plumbaginifolia
          Length = 477

 Score = 28.7 bits (61), Expect = 4.2
 Identities = 15/53 (28%), Positives = 26/53 (49%)
 Frame = +2

Query: 479 LLLNCSSDTLRVCHR*IITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 637
           L+   S +T+    +  +T N N  +  +V P  P    K +L+ H G ++DE
Sbjct: 248 LVKESSHETVAEKTKLALTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300


>At4g37290.1 68417.m05277 expressed protein
          Length = 84

 Score = 28.7 bits (61), Expect = 4.2
 Identities = 13/28 (46%), Positives = 16/28 (57%)
 Frame = -2

Query: 361 ETEDTFIADLVVGLSTGQIKTGAPCRSE 278
           +TE+ F+A L  GLS G IK   P   E
Sbjct: 31  KTEEKFVASLFDGLSLGSIKDSGPSPGE 58


>At3g59870.1 68416.m06681 expressed protein hypothetical protein
           F6E13.7 - Arabidopsis thaliana, PIR:T00674
          Length = 288

 Score = 28.7 bits (61), Expect = 4.2
 Identities = 15/46 (32%), Positives = 19/46 (41%)
 Frame = -2

Query: 622 PMVSIEDPFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVE 485
           PM   E   DQDD  +W N       +   D+ T T   RI T  +
Sbjct: 66  PMCFPEQTRDQDDVDSWVNKEDNVTCRFDSDEDTTTTGLRIPTQAQ 111


>At4g27060.1 68417.m03891 expressed protein
          Length = 864

 Score = 27.5 bits (58), Expect = 9.7
 Identities = 14/44 (31%), Positives = 21/44 (47%)
 Frame = -2

Query: 607 EDPFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVEKKA 476
           E P  +  W A  +      I++ G+D  V  P R+A A E +A
Sbjct: 665 EGPSARSVWQASKDEATLEAIRVAGEDGAVPRPTRVAVAPEAEA 708


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,042,282
Number of Sequences: 28952
Number of extensions: 336605
Number of successful extensions: 753
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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