BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10d04r (731 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 254 4e-68 At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ... 254 6e-68 At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 195 2e-50 At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 30 1.4 At5g16290.2 68418.m01904 acetolactate synthase small subunit, pu... 29 4.2 At5g16290.1 68418.m01903 acetolactate synthase small subunit, pu... 29 4.2 At4g37290.1 68417.m05277 expressed protein 29 4.2 At3g59870.1 68416.m06681 expressed protein hypothetical protein ... 29 4.2 At4g27060.1 68417.m03891 expressed protein 27 9.7 >At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot:P15007 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Drosophila melanogaster] Length = 477 Score = 254 bits (622), Expect = 4e-68 Identities = 120/171 (70%), Positives = 142/171 (83%) Frame = -2 Query: 703 KNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGRTPIQIVGDDL 524 K ++ LS++ LAD+Y +FIKDFP+VSIEDPFDQDDWS+WA+L IQ+VGDDL Sbjct: 308 KKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDL 367 Query: 523 TVTNPKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETEDTF 344 VTNPKRIA A++K++CN LLLKVNQIG+VTESI A L +K GWG MVSHRSGETED F Sbjct: 368 LVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNF 427 Query: 343 IADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGVNAKYAGKNFRRP 191 IADL VGL++GQIKTGAPCRSERL+KYNQ+LRIEEELG N +YAG+ FR P Sbjct: 428 IADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELG-NVRYAGEAFRSP 477 >At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Arabidopsis thaliana] Length = 444 Score = 254 bits (621), Expect = 6e-68 Identities = 120/174 (68%), Positives = 140/174 (80%), Gaps = 2/174 (1%) Frame = -2 Query: 703 KNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGR--TPIQIVGD 530 K ++N +S D L D+Y F+ ++P+VSIEDPFDQDDW +A +T T +QIVGD Sbjct: 268 KEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQDDWEHYAKMTTECGTEVQIVGD 327 Query: 529 DLTVTNPKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETED 350 DL VTNPKR+A A+ +K+CN LLLKVNQIGSVTESI+A ++KK GWG M SHRSGETED Sbjct: 328 DLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEAVKMSKKAGWGVMTSHRSGETED 387 Query: 349 TFIADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGVNAKYAGKNFRRPV 188 TFIADL VGLSTGQIKTGAPCRSERLAKYNQ+LRIEEELG A YAG NFR+PV Sbjct: 388 TFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEELGSEAIYAGVNFRKPV 441 >At2g29560.1 68415.m03590 enolase, putative similar to enolase [Spinacia oleracea] gi|8919731|emb|CAB96173 Length = 475 Score = 195 bits (476), Expect = 2e-50 Identities = 87/170 (51%), Positives = 125/170 (73%) Frame = -2 Query: 706 LKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGRTPIQIVGDD 527 +K+P+ + ++ S++ + D+Y + D+P+VSIEDPFD++DW + Q+VGDD Sbjct: 303 IKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVGDD 362 Query: 526 LTVTNPKRIATAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETEDT 347 L ++N KR+ A+++ +CN LLLKVNQIG+VTE+I+ +A+ WG + SHR GETED+ Sbjct: 363 LLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETEDS 422 Query: 346 FIADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGVNAKYAGKNFR 197 FI+DL VGL+TG IK GAPCR ER KYNQ+LRIEEELG A YAG++++ Sbjct: 423 FISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472 >At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain, PF04937: Protein of unknown function (DUF 659) Length = 433 Score = 30.3 bits (65), Expect = 1.4 Identities = 19/40 (47%), Positives = 22/40 (55%), Gaps = 4/40 (10%) Frame = +3 Query: 219 LALTPSSSSMRRIWLYLARRSER----HGAPVLI*PVDKP 326 L+LTPSSSS R W SER +G PV P +KP Sbjct: 358 LSLTPSSSSFERNWSAFEGPSERDEEPNGEPVYQTPNEKP 397 >At5g16290.2 68418.m01904 acetolactate synthase small subunit, putative similar to gi:5931761 from Nicotiana plumbaginifolia Length = 477 Score = 28.7 bits (61), Expect = 4.2 Identities = 15/53 (28%), Positives = 26/53 (49%) Frame = +2 Query: 479 LLLNCSSDTLRVCHR*IITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 637 L+ S +T+ + +T N N + +V P P K +L+ H G ++DE Sbjct: 248 LVKESSHETVAEKTKLALTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300 >At5g16290.1 68418.m01903 acetolactate synthase small subunit, putative similar to gi:5931761 from Nicotiana plumbaginifolia Length = 477 Score = 28.7 bits (61), Expect = 4.2 Identities = 15/53 (28%), Positives = 26/53 (49%) Frame = +2 Query: 479 LLLNCSSDTLRVCHR*IITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 637 L+ S +T+ + +T N N + +V P P K +L+ H G ++DE Sbjct: 248 LVKESSHETVAEKTKLALTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300 >At4g37290.1 68417.m05277 expressed protein Length = 84 Score = 28.7 bits (61), Expect = 4.2 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = -2 Query: 361 ETEDTFIADLVVGLSTGQIKTGAPCRSE 278 +TE+ F+A L GLS G IK P E Sbjct: 31 KTEEKFVASLFDGLSLGSIKDSGPSPGE 58 >At3g59870.1 68416.m06681 expressed protein hypothetical protein F6E13.7 - Arabidopsis thaliana, PIR:T00674 Length = 288 Score = 28.7 bits (61), Expect = 4.2 Identities = 15/46 (32%), Positives = 19/46 (41%) Frame = -2 Query: 622 PMVSIEDPFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVE 485 PM E DQDD +W N + D+ T T RI T + Sbjct: 66 PMCFPEQTRDQDDVDSWVNKEDNVTCRFDSDEDTTTTGLRIPTQAQ 111 >At4g27060.1 68417.m03891 expressed protein Length = 864 Score = 27.5 bits (58), Expect = 9.7 Identities = 14/44 (31%), Positives = 21/44 (47%) Frame = -2 Query: 607 EDPFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVEKKA 476 E P + W A + I++ G+D V P R+A A E +A Sbjct: 665 EGPSARSVWQASKDEATLEAIRVAGEDGAVPRPTRVAVAPEAEA 708 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,042,282 Number of Sequences: 28952 Number of extensions: 336605 Number of successful extensions: 753 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 738 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 752 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1604469728 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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