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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10d04f
         (624 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot...   229   2e-60
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ...   198   3e-51
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S...   178   2e-45
At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) fa...    29   1.9  
At3g55110.1 68416.m06120 ABC transporter family protein ATP-bind...    29   3.3  
At1g64050.1 68414.m07255 expressed protein                             28   5.8  
At4g15420.1 68417.m02359 PRLI-interacting factor K nearly identi...    27   7.7  
At1g76520.2 68414.m08905 auxin efflux carrier family protein con...    27   7.7  
At1g76520.1 68414.m08904 auxin efflux carrier family protein con...    27   7.7  

>At1g74030.1 68414.m08573 enolase, putative similar to
           Swiss-Prot:P15007 enolase (EC 4.2.1.11)
           (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
           hydro-lyase) [Drosophila melanogaster]
          Length = 477

 Score =  229 bits (559), Expect = 2e-60
 Identities = 113/168 (67%), Positives = 134/168 (79%)
 Frame = +3

Query: 120 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGV 299
           +K +KARQI DSRGNPTVEVDL+T+  L+R+AVPSGASTG++EALELRD  KS Y GKGV
Sbjct: 51  VKGVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 109

Query: 300 LTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXXXX 479
           L AIKNINEL+AP+L    ++V  Q ++D LML+LDGT NKSKLGANAILGVSL      
Sbjct: 110 LQAIKNINELVAPKLI--GVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAG 167

Query: 480 XXXXNVPLYKHLADLAGNNDIVLPVPAFNVINGGSHAGNKLAMQEFMI 623
                VPLYKH+ + +G  ++V+PVPAFNVINGGSHAGN LAMQEFMI
Sbjct: 168 AGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMI 215


>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
           enolase (EC 4.2.1.11) (2-phosphoglycerate
           dehydratase)(2-phospho-D- glycerate hydro-lyase)
           [Arabidopsis thaliana]
          Length = 444

 Score =  198 bits (482), Expect = 3e-51
 Identities = 110/175 (62%), Positives = 126/175 (72%), Gaps = 7/175 (4%)
 Frame = +3

Query: 120 IKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGASTGVHEALELRDNIKSEYHGKG 296
           I  +KARQIFDSRGNPTVEVD+ T  G+   AAVPSGASTG++EALELRD   S+Y GKG
Sbjct: 4   ITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDG-GSDYLGKG 62

Query: 297 VLTAIKNINELIAPELTKANLEVTQQREIDELML-KLDGTEN-----KSKLGANAILGVS 458
           V  A+ N+N +I P L     + TQQ  ID  M+ +LDGT+N     K KLGANAIL VS
Sbjct: 63  VSKAVGNVNNIIGPALI--GKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 120

Query: 459 LXXXXXXXXXXNVPLYKHLADLAGNNDIVLPVPAFNVINGGSHAGNKLAMQEFMI 623
           L           +PLYKH+A+LAGN  IVLPVPAFNVINGGSHAGNKLAMQEFMI
Sbjct: 121 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMI 175


>At2g29560.1 68415.m03590 enolase, putative similar to enolase
           [Spinacia oleracea] gi|8919731|emb|CAB96173
          Length = 475

 Score =  178 bits (434), Expect = 2e-45
 Identities = 97/183 (53%), Positives = 123/183 (67%)
 Frame = +3

Query: 75  ISLNLRKSSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEAL 254
           I+ +L+K+ S    VI  +KARQI DSRG PTVEVDL T  G+FRA+VPSG S+G +EA+
Sbjct: 35  IANHLKKAVS---SVITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAI 91

Query: 255 ELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLG 434
           ELRD  K  Y G  V  A+KNINE I+  L    ++   Q +ID+ M+ LD TE KS+LG
Sbjct: 92  ELRDGDKGMYLGNSVAKAVKNINEKISEALI--GMDPKLQGQIDQAMIDLDKTEKKSELG 149

Query: 435 ANAILGVSLXXXXXXXXXXNVPLYKHLADLAGNNDIVLPVPAFNVINGGSHAGNKLAMQE 614
           ANAIL VS+           VPL KHL+DL+G  ++VLPVPAF V++GG HA N  A+QE
Sbjct: 150 ANAILAVSIAACKAGAAEKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQE 209

Query: 615 FMI 623
            MI
Sbjct: 210 IMI 212


>At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger)
           family protein contains a Zinc finger, C3HC4 type (RING
           finger) signature, PROSITE:PS00518
          Length = 688

 Score = 29.5 bits (63), Expect = 1.9
 Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 3/53 (5%)
 Frame = +3

Query: 246 EALELRDNIKSEYHGKG--VLTAIKNINELIA-PELTKANLEVTQQREIDELM 395
           E+ E+ D +  EY  K   +LT +K++   ++ PELT+  L++  Q + DEL+
Sbjct: 143 ESEEIADGV-DEYETKEDIMLTILKDLRSSVSEPELTEEQLKMNDQLQEDELL 194


>At3g55110.1 68416.m06120 ABC transporter family protein ATP-binding
           cassette-sub-family G-member 2, Mus musculus,
           EMBL:AF140218
          Length = 708

 Score = 28.7 bits (61), Expect = 3.3
 Identities = 16/71 (22%), Positives = 30/71 (42%)
 Frame = +3

Query: 147 FDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINE 326
           F S G P  E + +TE  L       G+S G  + +E  +  +     +    +  ++ E
Sbjct: 308 FSSFGRPIPEKENITEFALDVIRELEGSSEGTRDLVEFNEKWQQNQTARATTQSRVSLKE 367

Query: 327 LIAPELTKANL 359
            IA  +++  L
Sbjct: 368 AIAASVSRGKL 378


>At1g64050.1 68414.m07255 expressed protein
          Length = 668

 Score = 27.9 bits (59), Expect = 5.8
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = -3

Query: 241 TPVDAPEGTAARNKPSSVTRSTSTVGLPRESKI 143
           +P+ +PE   +  K   ++RS+S  G PR +K+
Sbjct: 107 SPIASPESAESPRKRGKLSRSSSGNGTPRRTKL 139


>At4g15420.1 68417.m02359 PRLI-interacting factor K nearly identical
           to PRLI-interacting factor K [Arabidopsis thaliana]
           GI:11139266; contains Pfam profiles PF03152: Ubiquitin
           fusion degradation protein UFD1, PF00096: Zinc finger,
           C2H2 type
          Length = 561

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 20/89 (22%), Positives = 42/89 (47%)
 Frame = +3

Query: 114 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGK 293
           +V +SI   ++ +   + + +VD V E    + ++PS  S  +HE    R N+   +HG 
Sbjct: 377 LVQESIDGAKVGERAVSSSSDVDTV-ECRNCKHSIPS-RSIALHEVYCSRHNVVCNHHGC 434

Query: 294 GVLTAIKNINELIAPELTKANLEVTQQRE 380
           G++  ++     +  E     L+ T+  +
Sbjct: 435 GIVLRVEEAKNHLHCEKCGKALQPTEMEK 463


>At1g76520.2 68414.m08905 auxin efflux carrier family protein
           contains auxin efflux carrier domain, Pfam:PF03547
          Length = 390

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 14/48 (29%), Positives = 26/48 (54%)
 Frame = +3

Query: 129 IKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNI 272
           +K  ++F +   P VE+ L+T +G + A    G +   H+A +  +NI
Sbjct: 2   VKLLELFITSSKPVVEILLITSVGFYMAL--DGVNLLGHDARKYLNNI 47


>At1g76520.1 68414.m08904 auxin efflux carrier family protein
           contains auxin efflux carrier domain, Pfam:PF03547
          Length = 390

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 14/48 (29%), Positives = 26/48 (54%)
 Frame = +3

Query: 129 IKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNI 272
           +K  ++F +   P VE+ L+T +G + A    G +   H+A +  +NI
Sbjct: 2   VKLLELFITSSKPVVEILLITSVGFYMAL--DGVNLLGHDARKYLNNI 47


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,862,101
Number of Sequences: 28952
Number of extensions: 235351
Number of successful extensions: 588
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 576
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 582
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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