BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10c22r (342 letters) Database: rice 37,544 sequences; 14,793,348 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value 03_05_0107 - 20883353-20883627,20883739-20883801,20884608-208847... 73 8e-14 08_01_0417 - 3692247-3692329,3692442-3692504,3693233-3693350,369... 71 2e-13 06_01_0108 - 860080-860162,860276-860338,860857-860974,861481-86... 63 5e-11 03_06_0582 - 34891613-34891795,34892407-34892466,34893134-348932... 45 1e-05 04_04_1623 - 34846056-34846223,34846547-34846644,34846738-348468... 42 1e-04 03_01_0463 + 3551764-3551897,3551989-3552046,3552148-3552255,355... 29 0.72 04_01_0396 + 5191653-5191874,5192281-5192396,5192891-5193455 29 0.95 01_05_0573 + 23359968-23361044,23361112-23361255,23361458-23361841 29 1.3 11_06_0715 + 26552962-26553510,26553578-26555746 28 1.7 07_01_0703 - 5295561-5297523,5299344-5299462 28 1.7 05_02_0150 - 7111090-7111225,7111251-7111458,7111705-7111774,711... 27 2.9 08_02_0818 - 21524539-21524664,21524911-21524961,21525800-215261... 27 3.9 07_01_0751 - 5777017-5778032,5779718-5780111,5780830-5781936 27 3.9 03_05_0440 + 24322332-24322388,24323478-24323615,24323654-243237... 27 3.9 01_04_0143 + 16689471-16689498,16689687-16689818,16689974-166900... 27 3.9 12_02_0691 - 22195142-22196197 27 5.1 03_06_0051 + 31290431-31290610,31290727-31290849,31291771-312919... 27 5.1 02_01_0365 + 2621302-2621711,2622442-2622620,2623381-2623496,262... 27 5.1 01_07_0064 + 40834649-40834786,40834934-40834997,40835333-408353... 27 5.1 11_04_0348 - 16632102-16632188,16632326-16632391,16632487-166325... 26 6.7 10_01_0307 + 3382432-3382608,3382690-3382863,3383600-3383695,338... 26 6.7 09_04_0689 - 19480409-19480599,19480780-19480889,19481032-194811... 26 6.7 07_03_0191 - 14920321-14920511,14920844-14921177 26 6.7 04_01_0081 - 890347-890603,890871-891204 26 6.7 02_05_0753 + 31510269-31510473,31510989-31511035,31511123-315111... 26 6.7 11_06_0368 + 22756988-22758205 26 8.9 10_08_0931 - 21647562-21649037 26 8.9 09_03_0179 - 13131404-13131514,13131834-13131920,13132316-131324... 26 8.9 01_06_1208 + 35424241-35424370,35424415-35424728,35424782-354249... 26 8.9 >03_05_0107 - 20883353-20883627,20883739-20883801,20884608-20884725, 20885012-20885023 Length = 155 Score = 72.5 bits (170), Expect = 8e-14 Identities = 34/87 (39%), Positives = 52/87 (59%), Gaps = 2/87 (2%) Frame = -1 Query: 282 MSLDEQFKQVADKVRNWKTKPSDDENLALYSLYKQATIGDVNIAQPSGLV--ESAKWKAW 109 M L E F++ A+KV+ S+++ L LY LYKQAT+GDVN ++P + AKW AW Sbjct: 1 MGLQEDFEEYAEKVKTLPESTSNEDKLILYGLYKQATVGDVNTSRPGIFAQRDRAKWDAW 60 Query: 108 NGRKGISQDDAKKQYIENAEKLHSKYA 28 +G S+++A YI ++L + A Sbjct: 61 KAVEGKSKEEAMSDYITKVKQLQEEAA 87 >08_01_0417 - 3692247-3692329,3692442-3692504,3693233-3693350, 3694084-3694095 Length = 91 Score = 71.3 bits (167), Expect = 2e-13 Identities = 34/82 (41%), Positives = 48/82 (58%), Gaps = 2/82 (2%) Frame = -1 Query: 282 MSLDEQFKQVADKVRNWKTKPSDDENLALYSLYKQATIGDVNIAQPSGLV--ESAKWKAW 109 M L E F+Q A+K + S++ L LY LYKQAT+GDVN A+P + AKW AW Sbjct: 1 MGLQEDFEQYAEKAKTLPESTSNENKLILYGLYKQATVGDVNTARPGIFAQRDRAKWDAW 60 Query: 108 NGRKGISQDDAKKQYIENAEKL 43 +G S+++A YI ++L Sbjct: 61 KAVEGKSKEEAMSDYITKVKQL 82 >06_01_0108 - 860080-860162,860276-860338,860857-860974,861481-861492 Length = 91 Score = 63.3 bits (147), Expect = 5e-11 Identities = 30/82 (36%), Positives = 47/82 (57%), Gaps = 2/82 (2%) Frame = -1 Query: 282 MSLDEQFKQVADKVRNWKTKPSDDENLALYSLYKQATIGDVNIAQPS--GLVESAKWKAW 109 M L E+F++ A+K + S+++ L LY LYKQAT+G V +P L + KW AW Sbjct: 1 MGLQEEFEEFAEKAKTLPDTISNEDKLLLYGLYKQATVGPVTTGRPGIFNLKDRYKWDAW 60 Query: 108 NGRKGISQDDAKKQYIENAEKL 43 +G S+++A YI ++L Sbjct: 61 KAVEGKSKEEAMADYITKVKQL 82 >03_06_0582 - 34891613-34891795,34892407-34892466,34893134-34893280, 34893502-34893657,34893745-34893828,34893907-34893995, 34894575-34894689,34894776-34894899,34895080-34895219, 34895868-34895929,34896042-34896108,34896185-34896295, 34896868-34896945,34897074-34897274,34897492-34897563, 34897630-34897693,34898084-34898154,34898328-34898474 Length = 656 Score = 45.2 bits (102), Expect = 1e-05 Identities = 20/59 (33%), Positives = 35/59 (59%), Gaps = 2/59 (3%) Frame = -1 Query: 216 DDENLALYSLYKQATIGDVNIAQPSGL--VESAKWKAWNGRKGISQDDAKKQYIENAEK 46 +D L LY LY+QAT+G N+ +P VE +KW +W+G + +A + +++ E+ Sbjct: 37 NDVALLLYGLYQQATVGPCNVPKPRAWNPVEQSKWTSWHGLGSMPSAEAMRLFVKILEE 95 >04_04_1623 - 34846056-34846223,34846547-34846644,34846738-34846829, 34847096-34847202,34847385-34847490,34848108-34848547 Length = 336 Score = 41.9 bits (94), Expect = 1e-04 Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Frame = -1 Query: 228 TKPSDDENLALYSLYKQATIGDVNIAQPSG--LVESAKWKAWNGRKGISQDDAKKQYIEN 55 T + L LY LYK AT G QPS L AKW AW+ + ++A ++YI Sbjct: 109 TSVPEQAQLQLYGLYKIATEGPCTAPQPSALKLKARAKWNAWHKLGAMPTEEAMQKYITV 168 Query: 54 AEKLHSKYA 28 ++L ++ Sbjct: 169 VDELFPNWS 177 >03_01_0463 + 3551764-3551897,3551989-3552046,3552148-3552255, 3552664-3552721,3552788-3553014,3553085-3553145, 3553311-3553615 Length = 316 Score = 29.5 bits (63), Expect = 0.72 Identities = 20/93 (21%), Positives = 39/93 (41%) Frame = -1 Query: 315 TLPPDLKR*HKMSLDEQFKQVADKVRNWKTKPSDDENLALYSLYKQATIGDVNIAQPSGL 136 T+ PD + DE + A++ + KP+ + +SLY AT ++ + Sbjct: 189 TVFPDAEARLSQPKDETWSDCAEQ--GFAVKPTKGSAVLFFSLYPNATFDPGSLHGSCPV 246 Query: 135 VESAKWKAWNGRKGISQDDAKKQYIENAEKLHS 37 ++ KW A S D+ ++ + E H+ Sbjct: 247 IQGEKWSATKWIHVRSYDENGRRSSDKCEDQHA 279 >04_01_0396 + 5191653-5191874,5192281-5192396,5192891-5193455 Length = 300 Score = 29.1 bits (62), Expect = 0.95 Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 1/42 (2%) Frame = -2 Query: 341 TVPIGTLQLPYLLISKDNT-KCLSTSNSNRSPIRLGTGRPSP 219 ++ L + +L+IS NT K L++ S+ SP +LG G P P Sbjct: 114 SISASLLGIIFLIISLRNTNKGLTSLQSSSSPTKLGDGLPLP 155 >01_05_0573 + 23359968-23361044,23361112-23361255,23361458-23361841 Length = 534 Score = 28.7 bits (61), Expect = 1.3 Identities = 19/62 (30%), Positives = 28/62 (45%) Frame = +2 Query: 98 LRPFHAFHLALSTRPLGWAMLTSPMVACLYREYSARFSSSLGLVFQFLTLSATCLNCSSR 277 LR + +A+ R L + + SP++ CL YS R L L L C+ C + Sbjct: 230 LRELGLYCVAMEDRDLDFVLANSPVLECLGIYYSQRQIVLLRLASHSLRCVQICM-CIAE 288 Query: 278 DI 283 DI Sbjct: 289 DI 290 >11_06_0715 + 26552962-26553510,26553578-26555746 Length = 905 Score = 28.3 bits (60), Expect = 1.7 Identities = 15/32 (46%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +1 Query: 181 LVQGVQRKVLIVTGLGLP---VPNLIGDLFEL 267 LV G R + ++ +GLP +PN+IGDLF L Sbjct: 560 LVAGKCRYMSVLELIGLPKDNIPNVIGDLFNL 591 >07_01_0703 - 5295561-5297523,5299344-5299462 Length = 693 Score = 28.3 bits (60), Expect = 1.7 Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 2/45 (4%) Frame = +2 Query: 185 YREYSARFSSSLGLVFQFLTLSAT--CLNCSSRDILCYLLRSGGR 313 +RE A+ + +G + Q L+ C NCS +L L +S GR Sbjct: 454 FREDDAQLAEEIGDLVQLRVLNVVLNCSNCSEAKVLTELAKSMGR 498 >05_02_0150 - 7111090-7111225,7111251-7111458,7111705-7111774, 7112290-7112367 Length = 163 Score = 27.5 bits (58), Expect = 2.9 Identities = 14/46 (30%), Positives = 18/46 (39%) Frame = -2 Query: 164 MLTLPSPAVLWRAPSGRHGTVAKASPKTMPRSNTSKMRRNSTPNTH 27 M+ SP W AP G G +A+ P+ S NTH Sbjct: 35 MVINSSPKHTWFAPVGNKGALARIQPQVTELEGKVTSLSTSMENTH 80 >08_02_0818 - 21524539-21524664,21524911-21524961,21525800-21526170, 21526497-21526852,21527926-21527996 Length = 324 Score = 27.1 bits (57), Expect = 3.9 Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 4/32 (12%) Frame = -1 Query: 255 VADKVRNWKTKP----SDDENLALYSLYKQAT 172 V D++ W P +D+EN + +LYK+AT Sbjct: 241 VKDRIEEWYRPPWDRTADEENASFNALYKEAT 272 >07_01_0751 - 5777017-5778032,5779718-5780111,5780830-5781936 Length = 838 Score = 27.1 bits (57), Expect = 3.9 Identities = 20/69 (28%), Positives = 30/69 (43%), Gaps = 1/69 (1%) Frame = +1 Query: 19 NNLCVFGVEFLRIFDVLLLGIVLGDAFATVP-CLPLGALHKTAGLGNVNITYGSLLVQGV 195 NN C F + L++F L L +FA C LG +T L +V G+ L G Sbjct: 76 NNYCKFDDQRLQLFTSSLYLAALAASFAASRLCTRLGR-RRTMQLASVFFLGGTALCAGA 134 Query: 196 QRKVLIVTG 222 +++ G Sbjct: 135 ANLAMLIVG 143 >03_05_0440 + 24322332-24322388,24323478-24323615,24323654-24323736, 24325038-24325128,24326091-24326189,24326356-24326418, 24327182-24327259,24329354-24329543,24329812-24330065, 24330159-24330531,24331193-24331276,24332085-24332371, 24332495-24332542,24333194-24333220,24333951-24333987, 24334063-24334250,24334343-24334521,24334595-24334877, 24335481-24335717,24335798-24335988,24336552-24336561, 24336844-24336984,24337073-24337288,24338437-24338727, 24338852-24339025 Length = 1272 Score = 27.1 bits (57), Expect = 3.9 Identities = 15/29 (51%), Positives = 20/29 (68%) Frame = -1 Query: 213 DENLALYSLYKQATIGDVNIAQPSGLVES 127 DE+ AL +L+ A + VNI QPS +VES Sbjct: 476 DESSALDALHTLADLS-VNILQPSSIVES 503 >01_04_0143 + 16689471-16689498,16689687-16689818,16689974-16690013, 16690214-16690280,16690349-16690431,16690755-16690821, 16692741-16692821,16693696-16693791,16694172-16694198 Length = 206 Score = 27.1 bits (57), Expect = 3.9 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -1 Query: 234 WKTKPSDDENLALYSLYKQATIGDVNIAQ 148 WK S ++NLA +++Y T V++ Q Sbjct: 138 WKVSRSSEQNLAFFAVYNMETTDIVSLYQ 166 >12_02_0691 - 22195142-22196197 Length = 351 Score = 26.6 bits (56), Expect = 5.1 Identities = 23/81 (28%), Positives = 32/81 (39%), Gaps = 3/81 (3%) Frame = +2 Query: 104 PFHAFHLALSTRPLGWAMLTSPMVACLYREYSARFSSSLG---LVFQFLTLSATCLNCSS 274 PF + LA P G PM+AC +A ++ L L + L A N S+ Sbjct: 57 PFSVYALAFLRAPRGLHQTPYPMLACAVAAPAALLAAVLAVPVLAADDVAL-AWAANASA 115 Query: 275 RDILCYLLRSGGRVVARFQLV 337 L + L +GG F V Sbjct: 116 AAALWWCLTNGGYTAVAFSRV 136 >03_06_0051 + 31290431-31290610,31290727-31290849,31291771-31291928, 31292395-31292497,31293077-31293172,31293367-31293432, 31293500-31293619,31293905-31293991 Length = 310 Score = 26.6 bits (56), Expect = 5.1 Identities = 13/46 (28%), Positives = 20/46 (43%) Frame = -1 Query: 249 DKVRNWKTKPSDDENLALYSLYKQATIGDVNIAQPSGLVESAKWKA 112 +K K KP + L YSL TI ++ +++ KW A Sbjct: 254 EKCIGLKVKPRKGDGLLFYSLMVNGTIDPTSLHGSCPVIKGEKWVA 299 >02_01_0365 + 2621302-2621711,2622442-2622620,2623381-2623496, 2623626-2623964,2624113-2624307,2624505-2624594, 2625288-2625518,2625632-2625952 Length = 626 Score = 26.6 bits (56), Expect = 5.1 Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 1/71 (1%) Frame = -1 Query: 240 RNWKTKPSDDENLALYSLYKQATIGDVNIAQPSGLVESAKWKAWNGRKGISQDDAK-KQY 64 R K K +DE+ + + K+A + I Q L+++ WNG S++ K ++ Sbjct: 90 RKHKQKRKNDESDEVSRMEKRARYLLIKIKQEQNLLDAYSGDGWNGH---SREKIKPEKE 146 Query: 63 IENAEKLHSKY 31 ++ A+K KY Sbjct: 147 LQRAKKQIMKY 157 >01_07_0064 + 40834649-40834786,40834934-40834997,40835333-40835391, 40835602-40835677,40835775-40835846,40837641-40837728, 40838239-40838497,40838766-40839000,40839447-40839470, 40839910-40839997,40840110-40840300,40841082-40841322, 40841803-40842556 Length = 762 Score = 26.6 bits (56), Expect = 5.1 Identities = 16/59 (27%), Positives = 25/59 (42%) Frame = -1 Query: 312 LPPDLKR*HKMSLDEQFKQVADKVRNWKTKPSDDENLALYSLYKQATIGDVNIAQPSGL 136 L P + K D FK+ D+V +W T D+ N+A Y + + P G+ Sbjct: 161 LSPRILEDFKAYADVCFKEFGDRVAHWIT--IDEPNVASIGSYDSGQLAPGRCSDPFGI 217 >11_04_0348 - 16632102-16632188,16632326-16632391,16632487-16632571, 16632951-16633044,16633417-16633474,16633767-16633807, 16633919-16634018 Length = 176 Score = 26.2 bits (55), Expect = 6.7 Identities = 12/47 (25%), Positives = 27/47 (57%) Frame = -1 Query: 171 IGDVNIAQPSGLVESAKWKAWNGRKGISQDDAKKQYIENAEKLHSKY 31 +G VN+ + K+ + ++ ++ D+A+ IE AEK+H+++ Sbjct: 75 LGKVNVMYEDDMELMVKFYQFVAKEEMAIDEAELDPIEFAEKIHAQH 121 >10_01_0307 + 3382432-3382608,3382690-3382863,3383600-3383695, 3384640-3384709,3385878-3386002,3387128-3387268, 3387362-3387519,3387644-3387803,3390136-3390213, 3390326-3390385,3390473-3390528,3391886-3392090, 3394107-3394192,3394296-3394406,3394536-3394607, 3395453-3395538,3395730-3395878,3396107-3396250, 3396335-3396442,3396997-3397027,3397609-3397713, 3398283-3398407,3398807-3398950,3399073-3399177, 3400748-3400799,3401549-3401696,3401936-3402130, 3402404-3402647,3403152-3403259,3403887-3404034, 3405467-3405525,3405876-3405945,3407008-3407333, 3407666-3409204,3410526-3410733,3410870-3410906, 3411024-3411072,3411143-3411271 Length = 2025 Score = 26.2 bits (55), Expect = 6.7 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = -1 Query: 342 DSTNWNLATTLPPDLKR*HKMSLDEQ 265 DS NWNL+ PP +K +M L+E+ Sbjct: 1756 DSPNWNLSFGCPPIIK--EEMKLNEE 1779 >09_04_0689 - 19480409-19480599,19480780-19480889,19481032-19481117, 19481410-19481523,19481697-19482066,19482216-19482346, 19482628-19482736,19482901-19483022,19483113-19483219, 19483447-19483551,19483654-19483812,19484071-19484126, 19484200-19484251,19486724-19486821,19487086-19487210 Length = 644 Score = 26.2 bits (55), Expect = 6.7 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = +1 Query: 235 VPNLIGDLFELLVERHFVLSF 297 VP G+ ++LL E+HFVL + Sbjct: 447 VPVTAGNFYKLLAEKHFVLLY 467 >07_03_0191 - 14920321-14920511,14920844-14921177 Length = 174 Score = 26.2 bits (55), Expect = 6.7 Identities = 17/44 (38%), Positives = 19/44 (43%), Gaps = 4/44 (9%) Frame = -2 Query: 155 LPSPA-VLWRAP---SGRHGTVAKASPKTMPRSNTSKMRRNSTP 36 L PA V WR P SGRH T A P + T +R P Sbjct: 46 LSDPAQVWWRCPLPASGRHATTVAALAALSPDTMTHPLRLRPLP 89 >04_01_0081 - 890347-890603,890871-891204 Length = 196 Score = 26.2 bits (55), Expect = 6.7 Identities = 17/44 (38%), Positives = 19/44 (43%), Gaps = 4/44 (9%) Frame = -2 Query: 155 LPSPA-VLWRAP---SGRHGTVAKASPKTMPRSNTSKMRRNSTP 36 L PA V WR P SGRH T A P + T +R P Sbjct: 46 LSDPAQVWWRCPLPASGRHATTVAALAALSPDTMTHPLRLRPLP 89 >02_05_0753 + 31510269-31510473,31510989-31511035,31511123-31511177, 31511282-31511354,31511441-31511528,31511658-31511740, 31511841-31511970,31512183-31512261,31512409-31512473, 31512581-31512682 Length = 308 Score = 26.2 bits (55), Expect = 6.7 Identities = 17/50 (34%), Positives = 27/50 (54%) Frame = -1 Query: 219 SDDENLALYSLYKQATIGDVNIAQPSGLVESAKWKAWNGRKGISQDDAKK 70 SD ALY +A IG + +A+P+ + ++A W G K ++ AKK Sbjct: 19 SDRRRSALYGGEGRARIGSLRVAEPA-VAKAAVWA--RGSKPVAPLRAKK 65 >11_06_0368 + 22756988-22758205 Length = 405 Score = 25.8 bits (54), Expect = 8.9 Identities = 13/39 (33%), Positives = 18/39 (46%) Frame = -3 Query: 139 SCGERQVEGMERSQRHLPRRCQEAIHRKCGETPLQIRIN 23 SC E + L RC+ A HR C +T L+ +N Sbjct: 221 SCAVCLTEFRDGETLRLLPRCRHAFHRGCIDTWLRAHVN 259 >10_08_0931 - 21647562-21649037 Length = 491 Score = 25.8 bits (54), Expect = 8.9 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -2 Query: 155 LPSPAVLWRAPSGRHGTVAKASPKTMPR 72 LPSP + WR+ SGR A + P P+ Sbjct: 214 LPSP-IPWRSRSGRFDASAPSPPSPSPK 240 >09_03_0179 - 13131404-13131514,13131834-13131920,13132316-13132448, 13133079-13133319,13133423-13133540,13133546-13133731, 13133864-13134049,13134443-13134652,13135171-13135347, 13135823-13135964,13136385-13136927,13137565-13138022 Length = 863 Score = 25.8 bits (54), Expect = 8.9 Identities = 12/24 (50%), Positives = 13/24 (54%) Frame = -2 Query: 113 HGTVAKASPKTMPRSNTSKMRRNS 42 HGT A K MP NTS+ NS Sbjct: 413 HGTSDAAGSKKMPTGNTSQNMTNS 436 >01_06_1208 + 35424241-35424370,35424415-35424728,35424782-35424967, 35425361-35425523,35425601-35425787,35425875-35426016 Length = 373 Score = 25.8 bits (54), Expect = 8.9 Identities = 18/64 (28%), Positives = 25/64 (39%) Frame = -2 Query: 269 SNSNRSPIRLGTGRPSPVTMRTLRCTPCTSRLP*VMLTLPSPAVLWRAPSGRHGTVAKAS 90 + S+RSP+ T +RL + L PA + A G HG AS Sbjct: 52 AGSSRSPVFSSLVEDDGETRHLQALDGADTRLRLFQMDLLDPASITPAVDGAHGVFHLAS 111 Query: 89 PKTM 78 P T+ Sbjct: 112 PLTL 115 Database: rice Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 14,793,348 Number of sequences in database: 37,544 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,003,406 Number of Sequences: 37544 Number of extensions: 247667 Number of successful extensions: 893 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 875 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 888 length of database: 14,793,348 effective HSP length: 73 effective length of database: 12,052,636 effective search space used: 482105440 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -