BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10c19f (654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25B8.17 |||peptidase family A22|Schizosaccharomyces pombe|ch... 43 4e-05 SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 3.1 SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 27 3.1 SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 27 3.1 SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 26 5.5 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 26 5.5 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 7.2 SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 25 7.2 >SPAC25B8.17 |||peptidase family A22|Schizosaccharomyces pombe|chr 1|||Manual Length = 295 Score = 42.7 bits (96), Expect = 4e-05 Identities = 22/110 (20%), Positives = 54/110 (49%), Gaps = 1/110 (0%) Frame = +2 Query: 254 QKKAGERHETMSNKDALMFPLVASCALFGLYIFFQFFSKEYINLLLTGYFFFLGVLALSH 433 Q++ E + ++ + A++FP+ L +Y+ ++ SKEYI L+L GY ++ Sbjct: 23 QEEEPEEKQLINKRLAVLFPIFGGVTLVLMYLALRYLSKEYIQLILQGYASLASIICFVR 82 Query: 434 LLSPIISF-LVPASIPNIPFHIHFTRGERDNKQDIINYKFTSYDVICLLI 580 +P +F + A++ +I + + + + +I+ + + + + I Sbjct: 83 SFNPKTTFGKITATMSSIAIALFYFKTKHWMASNILAWALAANSISIMRI 132 Score = 27.1 bits (57), Expect = 2.4 Identities = 11/37 (29%), Positives = 19/37 (51%) Frame = +2 Query: 539 NYKFTSYDVICLLISLCLGAWYLLKKHWIANNLFGIA 649 N K T + + S+ + +Y KHW+A+N+ A Sbjct: 85 NPKTTFGKITATMSSIAIALFYFKTKHWMASNILAWA 121 >SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1958 Score = 26.6 bits (56), Expect = 3.1 Identities = 7/23 (30%), Positives = 18/23 (78%) Frame = +2 Query: 485 PFHIHFTRGERDNKQDIINYKFT 553 PF+IH++R +R+ ++I+ +++ Sbjct: 928 PFNIHYSRNQREEAENILRRRYS 950 >SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1717 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +3 Query: 6 KTIRRGKLS*KLYFCIIFYKKWQIWPQKY 92 K + R K S +L FCI+F+K W+ +Y Sbjct: 64 KELARFK-SARLNFCIVFFKNWEALYSQY 91 >SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1|||Manual Length = 881 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +2 Query: 155 SIEGIAIAYLSLVIMAILPIFFGSFRS 235 +I+GI I Y ++ + I IFFG F S Sbjct: 403 TIDGIYIIYFDMLALIIPTIFFGFFGS 429 Score = 25.4 bits (53), Expect = 7.2 Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 1/45 (2%) Frame = -1 Query: 144 FSVTF-CIVSLTLSSMFIGISEAISAISYRK*CRNTIFKTTFPYG 13 F V F C + T+ S F AI Y K CR +F +P+G Sbjct: 210 FLVIFICYIFFTVLSFF----NVPDAIVYSKLCRGLMFYLLYPFG 250 >SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/38 (28%), Positives = 21/38 (55%) Frame = +2 Query: 80 ASEIPINIEDSVKETIQNVTEKPPSSIEGIAIAYLSLV 193 A + I ++ + E ++ E+PPS + I + LSL+ Sbjct: 79 AFRLQIASKEFLNELVRRFPERPPSRLNKIQVMILSLI 116 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/36 (30%), Positives = 19/36 (52%), Gaps = 1/36 (2%) Frame = -2 Query: 374 TPLRRIGRKY-IVRKEHMMQPTGTSEHLCWTLFHDA 270 T + G Y ++ K ++ P + LCWT+ +DA Sbjct: 591 TQCKSCGPSYRLLPKIELLLPCSGRDDLCWTILNDA 626 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/40 (25%), Positives = 21/40 (52%) Frame = +2 Query: 68 MADMASEIPINIEDSVKETIQNVTEKPPSSIEGIAIAYLS 187 + ++ IP I+DS K T+ TE+ S++ ++ + Sbjct: 126 LEQQSNNIPTGIQDSSKYTVNGPTERDNKSMKSFVRSFFT 165 >SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/40 (32%), Positives = 20/40 (50%) Frame = -3 Query: 622 PVLFKQVPSSKTERDEQTNHIIGCELVIDDILFVVSLTTG 503 P L + VP + +E+ ++ + DDI VVS TG Sbjct: 440 PELERSVPKEEKTLEEKKPSMVHDSVTSDDIAVVVSRATG 479 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,751,377 Number of Sequences: 5004 Number of extensions: 57067 Number of successful extensions: 183 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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