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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10c19f
         (654 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g03120.1 68415.m00265 signal peptide peptidase family protein...    95   3e-20
At4g33410.1 68417.m04748 signal peptide peptidase family protein...    51   6e-07
At3g28770.1 68416.m03591 expressed protein                             31   0.88 
At5g61730.1 68418.m07746 ABC transporter family protein contains...    29   3.6  
At4g25930.1 68417.m03729 hypothetical protein contains Pfam prof...    28   6.2  

>At2g03120.1 68415.m00265 signal peptide peptidase family protein
           contains Pfam domain PF04258: Membrane protein of
           unknown function (DUF435)
          Length = 344

 Score = 95.5 bits (227), Expect = 3e-20
 Identities = 55/157 (35%), Positives = 85/157 (54%)
 Frame = +2

Query: 182 LSLVIMAILPIFFGSFRSVKYLNEQKKAGERHETMSNKDALMFPLVASCALFGLYIFFQF 361
           L++++ A + ++ G FRSVK            ETMS + A+ FPLV S  L  L++ F+F
Sbjct: 28  LNVILTACITVYVGCFRSVKDTPPT-------ETMSKEHAMRFPLVGSAMLLSLFLLFKF 80

Query: 362 FSKEYINLLLTGYFFFLGVLALSHLLSPIISFLVPASIPNIPFHIHFTRGERDNKQDIIN 541
            SK+ +N +LT YFF LG++ALS  L P I   +P      P++ +         + +  
Sbjct: 81  LSKDLVNAVLTAYFFVLGIVALSATLLPAIRRFLPN-----PWNDNLIVWRFPYFKSL-E 134

Query: 542 YKFTSYDVICLLISLCLGAWYLLKKHWIANNLFGIAF 652
            +FT   V+  +      AWY  KKHW+ANN+ G++F
Sbjct: 135 VEFTKSQVVAGIPGTFFCAWYAWKKHWLANNILGLSF 171


>At4g33410.1 68417.m04748 signal peptide peptidase family protein
           contains Pfam domain PF04258: Membrane protein of
           unknown function (DUF435)
          Length = 372

 Score = 51.2 bits (117), Expect = 6e-07
 Identities = 44/159 (27%), Positives = 80/159 (50%), Gaps = 4/159 (2%)
 Frame = +2

Query: 185 SLVIMAILPIFFGSFRSVKYLNEQKKAGERHE---TMSNKDALMFPLVASCALFGLYIFF 355
           +L++ A+   F  +FR++ Y  E ++  +  E   T+ +  ALM P+++SC+L  L +F+
Sbjct: 17  TLIVTAVTVTFASAFRALNYGKEMERNRDFSEASITLDSSQALMIPVMSSCSL--LLMFY 74

Query: 356 QFFSKEYINLLLTGYFFFLGVLALSHLLSPIISFLVPASIPNIPFHIHFTRGERDNKQDI 535
            F S   ++ LLT +     V +L + LSP   ++      + PF    +R         
Sbjct: 75  LFSS---VSQLLTAFTAIASVSSLFYWLSPYAVYMKTQLGLSDPF---LSR--------C 120

Query: 536 INYKFTSYDVICLL-ISLCLGAWYLLKKHWIANNLFGIA 649
            +  FT    + L+  ++ + AW L+  HW+ NNL GI+
Sbjct: 121 CSKSFTRIQGLLLVACAMTVVAW-LISGHWVLNNLLGIS 158


>At3g28770.1 68416.m03591 expressed protein 
          Length = 2081

 Score = 30.7 bits (66), Expect = 0.88
 Identities = 23/77 (29%), Positives = 38/77 (49%)
 Frame = +2

Query: 74   DMASEIPINIEDSVKETIQNVTEKPPSSIEGIAIAYLSLVIMAILPIFFGSFRSVKYLNE 253
            D + E+  N E+S+K+  + V     SS + +     ++ I     +  GS  SVKY  +
Sbjct: 864  DRSVEVKANKEESMKKKREEVQRNDKSSTKEVRDFANNMDI----DVQKGSGESVKYKKD 919

Query: 254  QKKAGERHETMSNKDAL 304
            +KK G + E   NKD +
Sbjct: 920  EKKEGNKEE---NKDTI 933


>At5g61730.1 68418.m07746 ABC transporter family protein contains
           Pfam profile: PF00005 ABC transporter
          Length = 940

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +2

Query: 332 LFGLYIFFQFFSK-EYINLLLTGYFFFLGVLALSHLLSPIIS 454
           LFG+   F+FF K  ++ + L  + F   ++ L+  LS IIS
Sbjct: 293 LFGMMFQFEFFLKNSFVLVFLLFFLFQFNMIGLAFALSSIIS 334


>At4g25930.1 68417.m03729 hypothetical protein contains Pfam profile
           PF03478: Protein of unknown function (DUF295)
          Length = 434

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 17/44 (38%), Positives = 23/44 (52%)
 Frame = -1

Query: 147 GFSVTFCIVSLTLSSMFIGISEAISAISYRK*CRNTIFKTTFPY 16
           G S  FCI + + SS+   I  AI+  S+ K  R    K T+PY
Sbjct: 60  GHSEAFCIQASSGSSLTASILWAITLRSHSKQVRMFSSKPTYPY 103


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,873,747
Number of Sequences: 28952
Number of extensions: 277462
Number of successful extensions: 778
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 755
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 778
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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