BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10c01r
(720 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protei... 121 4e-28
At5g28140.1 68418.m03398 hypothetical protein 29 4.1
At1g63520.1 68414.m07181 expressed protein 28 7.2
At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) / c... 27 9.5
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 27 9.5
>At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protein
contains weak similarity to vacuolar ATP synthase
subunit F (EC 3.6.3.14) (V-ATPase F subunit) (Vacuolar
proton pump F subunit) (V-ATPase 14 kDa subunit)
(Swiss-Prot:P50408) [Rattus norvegicus]; contains Pfam
PF01990: ATP synthase (F/14-kDa) subunit
Length = 128
Score = 121 bits (292), Expect = 4e-28
Identities = 53/112 (47%), Positives = 80/112 (71%)
Frame = -3
Query: 616 LISVIGDEDTCVGFLLGGIGEINKNRHPNFMVVDKNTPVSEIEECFKRFVKRXXXXXILI 437
LI++I DEDT VGFL+ G+G ++ R N+++VD T V +IE+ FK F R IL+
Sbjct: 14 LIAMIADEDTVVGFLMAGVGNVDIRRKTNYLIVDSKTTVRQIEDAFKEFSARDDIAIILL 73
Query: 436 NQNIAELIRHVIDAHSAPVPSVLEIPSKDHPYDASKDSILRRAKGMFNPDDL 281
+Q IA +IR ++D+++ PVP++LEIPSKDHPYD + DS+L R K +F+ + +
Sbjct: 74 SQYIANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLFSAESV 125
>At5g28140.1 68418.m03398 hypothetical protein
Length = 212
Score = 28.7 bits (61), Expect = 4.1
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 400 DAHSAPVPSVLEIPSKDHPYDASK 329
DAHS P+ ++ EI S+ HP A K
Sbjct: 137 DAHSRPMTNISEIASEKHPEPALK 160
>At1g63520.1 68414.m07181 expressed protein
Length = 528
Score = 27.9 bits (59), Expect = 7.2
Identities = 24/77 (31%), Positives = 39/77 (50%), Gaps = 2/77 (2%)
Frame = +2
Query: 395 RINHMSDQFSN--VLIDENDVNVIAFNKTLETFLDFTHRCILIDHHEIRMSVFIDLTDAP 568
RI MS+ FSN L+ E+ + + NK+ L H + +++ E+ M VFI D P
Sbjct: 150 RIKKMSNPFSNRNPLLPESGQEMFSRNKSSPVHL---HAHLRMEY-ELGMPVFIFSLDLP 205
Query: 569 E*ESDASVLIPDNTNQF 619
+ AS + D ++F
Sbjct: 206 DDVYMASTRMDDKESRF 222
>At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) /
cytochrome P450 identical to GA3 [Arabidopsis thaliana]
GI:3342249; similar to ent-kaurene oxidase [Cucurbita
maxima] GI:11934675; contains Pfam profile PF00067:
Cytochrome P450
Length = 509
Score = 27.5 bits (58), Expect = 9.5
Identities = 23/93 (24%), Positives = 39/93 (41%), Gaps = 3/93 (3%)
Frame = -3
Query: 607 VIGDEDTCVGFLLGGIGEINKNRHPNFMVV---DKNTPVSEIEECFKRFVKRXXXXXILI 437
V+ +T ++ I+ + N + V DK+ + + F + VKR L+
Sbjct: 90 VLNSTETAKEAMVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNG-LL 148
Query: 436 NQNIAELIRHVIDAHSAPVPSVLEIPSKDHPYD 338
N + RH DA V S L ++DHP +
Sbjct: 149 GANAQKRKRHYRDALIENVSSKLHAHARDHPQE 181
>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
HMM hit: AMP-binding enzymes PF00501
Length = 691
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +2
Query: 446 DVNVIAFNKTLETFLDFTHRCILIDHH-EIRMSVFID 553
D + F KTLE DF + I H E R+++F D
Sbjct: 103 DYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFAD 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,826,284
Number of Sequences: 28952
Number of extensions: 312375
Number of successful extensions: 856
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 856
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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