BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10c01f (660 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protei... 121 4e-28 At5g28140.1 68418.m03398 hypothetical protein 29 3.6 At2g17930.1 68415.m02076 FAT domain-containing protein / phospha... 29 3.6 At1g63520.1 68414.m07181 expressed protein 28 6.3 At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) / c... 27 8.4 At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 27 8.4 >At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protein contains weak similarity to vacuolar ATP synthase subunit F (EC 3.6.3.14) (V-ATPase F subunit) (Vacuolar proton pump F subunit) (V-ATPase 14 kDa subunit) (Swiss-Prot:P50408) [Rattus norvegicus]; contains Pfam PF01990: ATP synthase (F/14-kDa) subunit Length = 128 Score = 121 bits (292), Expect = 4e-28 Identities = 52/112 (46%), Positives = 79/112 (70%) Frame = +1 Query: 106 LISVIGDEDTCVGFLLGGIGEINKNRHPNFMVVDKNTPVSEIEECFKRFVKRXXXXXXLI 285 LI++I DEDT VGFL+ G+G ++ R N+++VD T V +IE+ FK F R L+ Sbjct: 14 LIAMIADEDTVVGFLMAGVGNVDIRRKTNYLIVDSKTTVRQIEDAFKEFSARDDIAIILL 73 Query: 286 NQNIAELIRHVIDAHSAPVPSVLEIPSKDHPYDASKDSILRRAKGMFNPDDL 441 +Q IA +IR ++D+++ PVP++LEIPSKDHPYD + DS+L R K +F+ + + Sbjct: 74 SQYIANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLFSAESV 125 >At5g28140.1 68418.m03398 hypothetical protein Length = 212 Score = 28.7 bits (61), Expect = 3.6 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = +1 Query: 322 DAHSAPVPSVLEIPSKDHPYDASK 393 DAHS P+ ++ EI S+ HP A K Sbjct: 137 DAHSRPMTNISEIASEKHPEPALK 160 >At2g17930.1 68415.m02076 FAT domain-containing protein / phosphatidylinositol 3- and 4-kinase family protein contains Pfam profiles PF02259 FAT domain, PF00454 Phosphatidylinositol 3- and 4-kinase, PF02260: FATC domain Length = 3795 Score = 28.7 bits (61), Expect = 3.6 Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = -2 Query: 215 VFLSTTMKFGCLFLLISPMPPSK-NPTQVSSSPITLINFPLTAA*RAIFLDLV*FFKFIF 39 V+ S +KF L L+S MPPS+ +P+ + + ++N L + + I Sbjct: 2055 VWPSANVKFNYLEKLLSSMPPSQSDPSTALAQGLDVMNKVLEKQPHLFIRNNINQISQIL 2114 Query: 38 QWCFSHK 18 + CF HK Sbjct: 2115 EPCFKHK 2121 >At1g63520.1 68414.m07181 expressed protein Length = 528 Score = 27.9 bits (59), Expect = 6.3 Identities = 24/77 (31%), Positives = 39/77 (50%), Gaps = 2/77 (2%) Frame = -1 Query: 327 RINHMSDQFSN--VLIDENDVNVIAFNKTLETFLDFTHRCILIDHHEIRMSVFIDLTDAP 154 RI MS+ FSN L+ E+ + + NK+ L H + +++ E+ M VFI D P Sbjct: 150 RIKKMSNPFSNRNPLLPESGQEMFSRNKSSPVHL---HAHLRMEY-ELGMPVFIFSLDLP 205 Query: 153 E*ESDASVLIPDNTNQF 103 + AS + D ++F Sbjct: 206 DDVYMASTRMDDKESRF 222 >At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) / cytochrome P450 identical to GA3 [Arabidopsis thaliana] GI:3342249; similar to ent-kaurene oxidase [Cucurbita maxima] GI:11934675; contains Pfam profile PF00067: Cytochrome P450 Length = 509 Score = 27.5 bits (58), Expect = 8.4 Identities = 23/93 (24%), Positives = 39/93 (41%), Gaps = 3/93 (3%) Frame = +1 Query: 115 VIGDEDTCVGFLLGGIGEINKNRHPNFMVV---DKNTPVSEIEECFKRFVKRXXXXXXLI 285 V+ +T ++ I+ + N + V DK+ + + F + VKR L+ Sbjct: 90 VLNSTETAKEAMVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNG-LL 148 Query: 286 NQNIAELIRHVIDAHSAPVPSVLEIPSKDHPYD 384 N + RH DA V S L ++DHP + Sbjct: 149 GANAQKRKRHYRDALIENVSSKLHAHARDHPQE 181 >At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family protein / long-chain acyl-CoA synthetase family protein (LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens, LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam HMM hit: AMP-binding enzymes PF00501 Length = 691 Score = 27.5 bits (58), Expect = 8.4 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Frame = -1 Query: 276 DVNVIAFNKTLETFLDFTHRCILIDHH-EIRMSVFID 169 D + F KTLE DF + I H E R+++F D Sbjct: 103 DYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFAD 139 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,178,385 Number of Sequences: 28952 Number of extensions: 298878 Number of successful extensions: 832 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 797 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 832 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1383534864 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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