Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10b24f
(582 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 157 8e-40
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 108 7e-25
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 27 2.0
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 26 4.6
SPAPYUG7.05 |||delta-1-pyrroline-5-carboxylate reductase |Schizo... 25 6.1
SPBC146.09c |lsd1|swm1, saf110|histone demethylase SWIRM1|Schizo... 25 6.1
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 25 8.1
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 8.1
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 157 bits (382), Expect = 8e-40
Identities = 78/127 (61%), Positives = 97/127 (76%), Gaps = 3/127 (2%)
Frame = +2
Query: 209 VLSEIQQRCFSVSPLTAA--AAQVAMSKFDKVP-LPYEKLTKNLEVVKKRLGRELTLSEK 379
+ ++ R FS +P+ A A +VAMS F+K + Y+++ NLE+VKKRL R LT SEK
Sbjct: 5 IFTQSTLRSFSCAPVAANIDAKKVAMSNFEKNKFINYQRIKDNLEIVKKRLNRPLTYSEK 64
Query: 380 ILYSHLDDPKGQEIERGASYLRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDH 559
ILY HLDDP Q+IERG SYL+LRPDRVA QDATAQMA+LQF+S+G+P VAVP T+HCDH
Sbjct: 65 ILYGHLDDPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDH 124
Query: 560 LIEAQVG 580
LIEA G
Sbjct: 125 LIEAYEG 131
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 108 bits (259), Expect = 7e-25
Identities = 59/117 (50%), Positives = 78/117 (66%), Gaps = 7/117 (5%)
Frame = +2
Query: 251 LTAAAAQVAMSKFDKVPLPYEKLTKNLEVVKKRL-GRELTLSEKILYSHLDDPK------ 409
L AA Q S+ V PYEKL L+ V+K L G++LTL+EK+LYSHL +P+
Sbjct: 19 LVAARFQSTASRASYVTPPYEKLMGKLQQVRKFLPGQKLTLAEKVLYSHLVNPEESFSGV 78
Query: 410 GQEIERGASYLRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVG 580
RG+ YL+L PDRVAMQDA+AQMA+LQF++ GL + +P++IHCDHLI G
Sbjct: 79 SPSDIRGSLYLKLNPDRVAMQDASAQMALLQFMTCGLEKTMIPASIHCDHLIVGHRG 135
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 27.1 bits (57), Expect = 2.0
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 205 GGTL*NPTKMFQRISIDRRRGPGGDVQVRQGTLA 306
GG++ N + ++ ID R PG ++ V QG+ A
Sbjct: 1226 GGSIINKIRKIAQVKIDVPRTPGDEIVVVQGSRA 1259
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 25.8 bits (54), Expect = 4.6
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +2
Query: 281 SKFDKVPLPYEKLTKNLEVVKKR 349
+KFD+V +PY+ + ++E KR
Sbjct: 396 AKFDEVDMPYDTIWLDIEYASKR 418
>SPAPYUG7.05 |||delta-1-pyrroline-5-carboxylate reductase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 282
Score = 25.4 bits (53), Expect = 6.1
Identities = 18/72 (25%), Positives = 33/72 (45%)
Frame = +2
Query: 170 MRVLHGQGSRTRVVLSEIQQRCFSVSPLTAAAAQVAMSKFDKVPLPYEKLTKNLEVVKKR 349
+ +L G+ + + + R + P TA+ + +MS P E+ K E V
Sbjct: 109 LSILAGKTISSLQSMLDESTRVIRIMPNTASRIRESMSVICPGPNATEEDIKFAEWVFNG 168
Query: 350 LGRELTLSEKIL 385
+GR + L EK++
Sbjct: 169 IGRSMKLPEKLI 180
>SPBC146.09c |lsd1|swm1, saf110|histone demethylase
SWIRM1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1000
Score = 25.4 bits (53), Expect = 6.1
Identities = 20/84 (23%), Positives = 34/84 (40%), Gaps = 1/84 (1%)
Frame = +2
Query: 260 AAAQVAMSKFDKVPLPYEK-LTKNLEVVKKRLGRELTLSEKILYSHLDDPKGQEIERGAS 436
AA V S V LP + +NLE +K G + + + + K + I
Sbjct: 750 AARDVLASLIGNVVLPNTLVIEENLEQPRKTYGTKRNAQQALGKEGERENKEKRISYHTE 809
Query: 437 YLRLRPDRVAMQDATAQMAMLQFI 508
YLRLR R+ + + + + +
Sbjct: 810 YLRLRQKRLDKEQQECDLLIAELL 833
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 25.0 bits (52), Expect = 8.1
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = -1
Query: 237 KHLCWISESTTLVLLPWPCKTLIQCAMVAGLQYIKRINLEKF 112
++ CWIS TL+LL T +Q + L I RI F
Sbjct: 320 QYKCWISRDVTLILL-----TALQLVNIYWLILILRIGYRAF 356
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 25.0 bits (52), Expect = 8.1
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = -2
Query: 578 RLGLRSSDHNEWWTVQLHGEGRKR*IVTLPFVQWRLAWPHGRD 450
RLG + H + +L E K ++T P V +R+ + +GR+
Sbjct: 391 RLGFLGTLHLSVFIDRLKDEYGKELLITSPTVPYRIKYKNGRE 433
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,405,939
Number of Sequences: 5004
Number of extensions: 46998
Number of successful extensions: 114
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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