BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10b18r (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schi... 37 0.004 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 35 0.014 SPAC1751.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.2 SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe... 27 2.2 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 5.1 SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protei... 26 5.1 SPCC4E9.01c |rec11|SPCC550.16c|meiotic cohesin complex subunit R... 26 5.1 SPAC1F3.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 26 5.1 SPCP1E11.03 |mug170||arrestin|Schizosaccharomyces pombe|chr 3|||... 26 6.7 SPCC61.05 |||S. pombe specific multicopy membrane protein family... 26 6.7 SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 26 6.7 SPAC1782.04 |cox24||mitochondrial mRNA processing protein Cox24 ... 25 8.9 SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 25 8.9 >SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 36.7 bits (81), Expect = 0.004 Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 1/61 (1%) Frame = -3 Query: 351 PMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIHFHTPN-SNTLFDYVHKDDLPKDLG 175 P R+ + +VIN P ++L K L +KIH N + L + + D+LP LG Sbjct: 201 PERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGSNYKSALLEQIPADNLPAKLG 260 Query: 174 G 172 G Sbjct: 261 G 261 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 34.7 bits (76), Expect = 0.014 Identities = 29/94 (30%), Positives = 42/94 (44%), Gaps = 1/94 (1%) Frame = -3 Query: 345 RLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIHFHTPNSNTLFDYVHKDDLPKDLGGIR 166 RL + VIN P V + L P + +EK+ F+ P L YV KD L + GG Sbjct: 769 RLGRALVINIPWAVWGFFKLISPFIDPITREKLKFNEP----LDRYVPKDQLDSNFGGSL 824 Query: 165 DSLDIYTKKMKEMM-LTKRDVLARDDLWRKNTAK 67 + K +++ L K L + WRK +K Sbjct: 825 HFEYHHEKYWPQLVELCKSRRLGILEKWRKMGSK 858 >SPAC1751.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 123 Score = 27.5 bits (58), Expect = 2.2 Identities = 17/80 (21%), Positives = 35/80 (43%) Frame = -3 Query: 336 QIHVINTPLIVDKIYSLFKPLLKSDVKEKIHFHTPNSNTLFDYVHKDDLPKDLGGIRDSL 157 Q+ V++ +I +Y +K + V ++ + N +FD + DDL L + L Sbjct: 22 QVSVVDVTMINISLYKSYKSYPTNKVLSELAALFESCNLIFDLIWFDDLQNILNQVTYEL 81 Query: 156 DIYTKKMKEMMLTKRDVLAR 97 D K ++ + V ++ Sbjct: 82 DDKIKSKLQVAHEREAVFSK 101 >SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 652 Score = 27.5 bits (58), Expect = 2.2 Identities = 23/93 (24%), Positives = 43/93 (46%), Gaps = 7/93 (7%) Frame = +1 Query: 361 PLVFRYCFAENR---IILSNEFIVKILNV----YNNNVVVSQTIG*EPSVCGQQEQSRIM 519 P FRY F+ N L +F K + + YN++++V+ +G ++ + ++ R + Sbjct: 308 PEDFRYLFSGNTDDMFRLGVKFTRKTVKLFSQFYNSDIIVASPLGLRLAIGNKGDKKRDL 367 Query: 520 *SFKSIESRVVDLPNPYMFFIRRFINCHFHNLH 618 SIE +VD + + I C F L+ Sbjct: 368 DYLSSIEIAMVDQAHALVMQNWEHIECIFDELN 400 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 26.2 bits (55), Expect = 5.1 Identities = 12/56 (21%), Positives = 26/56 (46%) Frame = -3 Query: 669 PELFTNRDPLSPSIQETMKIVKMAVYETTDKKHIWIWQVNDPGFDRFETLHDSRLF 502 P +F + P + +++VK+ + ET K +++ + G + D RL+ Sbjct: 392 PAIFQDVKIPEPVVGNALEVVKLLLKETGGKAFVFVSALPTMGLGKLRHREDQRLY 447 >SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protein Mcp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 26.2 bits (55), Expect = 5.1 Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 1/64 (1%) Frame = -2 Query: 319 YTADSRQNIFVVQAPPQKRRKRKDTFSHAKF-EHTIRLRTQRRLTKGLGRDQRQSGYLYK 143 + DS Q +++ K +++ ++ K T+R +TQ L K G + ++GYLY+ Sbjct: 68 FERDSTQRTLQIESLESKIGEQERDLNNEKLASETLREKTQL-LEKENGALKVENGYLYE 126 Query: 142 KNER 131 K+ + Sbjct: 127 KSRK 130 >SPCC4E9.01c |rec11|SPCC550.16c|meiotic cohesin complex subunit Rec11|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 26.2 bits (55), Expect = 5.1 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 2/46 (4%) Frame = -2 Query: 322 KYTADSRQNIFVVQAPPQKRRK--RKDTFSHAKFEHTIRLRTQRRL 191 +Y S QN+ + +P +K+RK +D FS + E + T + Sbjct: 29 EYEGPSLQNVSICASPSEKKRKWGTQDEFSLTENEENNEIETNEEM 74 >SPAC1F3.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 108 Score = 26.2 bits (55), Expect = 5.1 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +1 Query: 562 NPYMFFIRRFINCHFHNLHSFLYR 633 +P+ F I+R H + LHSF+YR Sbjct: 78 SPHFFPIKRIRPFHENPLHSFVYR 101 >SPCP1E11.03 |mug170||arrestin|Schizosaccharomyces pombe|chr 3|||Manual Length = 426 Score = 25.8 bits (54), Expect = 6.7 Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 2/60 (3%) Frame = -3 Query: 186 KDLGGIRDSLD--IYTKKMKEMMLTKRDVLARDDLWRKNTAKPEADNAKSFRNLGID*LF 13 K L I + D + E++ K DVL + L RK++ PEAD NL + +F Sbjct: 21 KTLSSIENPFDDPYILSEESEILTIKTDVLRPNILNRKSSFYPEADAPVRKSNLQVKKIF 80 >SPCC61.05 |||S. pombe specific multicopy membrane protein family 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 469 Score = 25.8 bits (54), Expect = 6.7 Identities = 13/22 (59%), Positives = 14/22 (63%) Frame = +1 Query: 232 WRVKMYLFFYVAFEEGLEQRIY 297 W V +LF V FEEGLEQ Y Sbjct: 264 WYVVSFLFSLV-FEEGLEQSAY 284 >SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 25.8 bits (54), Expect = 6.7 Identities = 11/36 (30%), Positives = 23/36 (63%) Frame = -2 Query: 379 NIEIPRGGNADAFEADTRHKYTADSRQNIFVVQAPP 272 ++E PRG N +AFE + +++ + ++F + +PP Sbjct: 82 SVEKPRGRNMNAFEETSWNRFRKNG--SLFPLSSPP 115 >SPAC1782.04 |cox24||mitochondrial mRNA processing protein Cox24 |Schizosaccharomyces pombe|chr 1|||Manual Length = 175 Score = 25.4 bits (53), Expect = 8.9 Identities = 15/31 (48%), Positives = 19/31 (61%) Frame = -2 Query: 265 RRKRKDTFSHAKFEHTIRLRTQRRLTKGLGR 173 +RKRK + KF+ RLR QR L K LG+ Sbjct: 147 KRKRKLKMNKHKFKK--RLRRQRALRKRLGK 175 >SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Leu1|Schizosaccharomyces pombe|chr 2|||Manual Length = 371 Score = 25.4 bits (53), Expect = 8.9 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = -3 Query: 126 MLTKRDVLARDDLWRKNTAK 67 +L K +VLA LWRK AK Sbjct: 188 LLDKANVLATSRLWRKTVAK 207 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,948,552 Number of Sequences: 5004 Number of extensions: 60907 Number of successful extensions: 172 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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