Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10b18r
(758 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /... 36 0.029
At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / poly... 31 1.1
At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative / phos... 30 1.5
At1g72160.1 68414.m08343 SEC14 cytosolic factor family protein /... 30 1.5
At1g26830.1 68414.m03270 cullin, putative similar to Cullin homo... 30 1.9
At1g69670.1 68414.m08018 cullin, putative contains similarity to... 29 2.5
At1g22180.3 68414.m02773 SEC14 cytosolic factor family protein /... 29 2.5
At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /... 29 2.5
At1g22180.1 68414.m02772 SEC14 cytosolic factor family protein /... 29 2.5
At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative / phos... 29 3.4
At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosph... 29 3.4
At4g11080.1 68417.m01800 high mobility group (HMG1/2) family pro... 29 4.4
At3g50950.2 68416.m05579 disease resistance protein (CC-NBS-LRR ... 29 4.4
At3g50950.1 68416.m05578 disease resistance protein (CC-NBS-LRR ... 29 4.4
At4g36490.1 68417.m05181 SEC14 cytosolic factor, putative / phos... 28 5.9
At1g67140.1 68414.m07638 expressed protein 28 5.9
At2g38720.1 68415.m04755 microtubule associated protein (MAP65/A... 28 7.7
At1g48820.1 68414.m05463 terpene synthase/cyclase family protein... 28 7.7
At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 28 7.7
>At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max};; contains Pfam PF00650 : CRAL/TRIO
domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 409
Score = 35.9 bits (79), Expect = 0.029
Identities = 18/59 (30%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -3
Query: 324 INTPLIVDKIYSLFKPLLKSDVKEKIHFHTPN--SNTLFDYVHKDDLPKDLGGIRDSLD 154
IN P IYS+F P L K K + TL+ ++ +D+P GG+ D
Sbjct: 236 INVPWYFSVIYSMFSPFLTQRTKSKFVMSKEGNAAETLYKFIRPEDIPVQYGGLSRPTD 294
>At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative /
polyphosphoinositide-binding protein, putative similar
to polyphosphoinositide binding protein SEC14 homolog
Ssh1p (GI:2739044) {Glycine max}
Length = 341
Score = 30.7 bits (66), Expect = 1.1
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = -3
Query: 351 PMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIH 244
P + +V+N P I + + KPLL+ ++K+H
Sbjct: 184 PEKTNTYYVVNAPYIFSACWKVVKPLLQERTRKKVH 219
>At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus] and
phosphatidylinositol-phosphatidylcholine transfer
protein SEC14, Yarrowia lipolytica, PIR2:S43745;contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 614
Score = 30.3 bits (65), Expect = 1.5
Identities = 23/83 (27%), Positives = 33/83 (39%), Gaps = 1/83 (1%)
Frame = -3
Query: 417 KFITKYNSILGKTISKYQGGAMPMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIH-F 241
K TK L + K G P L Q+ +IN ++S K L KIH
Sbjct: 236 KNFTKSARELITRLQKIDGDNYPETLHQMFIINAGPGFRLLWSTVKSFLDPKTTSKIHVL 295
Query: 240 HTPNSNTLFDYVHKDDLPKDLGG 172
+ L + + +LP+ LGG
Sbjct: 296 GCKYQSKLLEIIDSSELPEFLGG 318
>At1g72160.1 68414.m08343 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
GI:807956 from [Saccharomyces cerevisiae]similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max}; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 490
Score = 30.3 bits (65), Expect = 1.5
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = -3
Query: 324 INTPLIVDKIYSLFKPLLKSDVKEKIHFHTPN--SNTLFDYVHKDDLPKDLGGI 169
IN P Y++ P + K K+ F P+ + TLF Y+ + +P GG+
Sbjct: 319 INVPWWYLVFYTVIGPFMTPRSKSKLVFAGPSRSAETLFKYISPEQVPVQYGGL 372
>At1g26830.1 68414.m03270 cullin, putative similar to Cullin homolog
3 (CUL-3) SP:Q13618, GI:3639052 from [Homo sapiens];
contains Pfam profile PF00888: Cullin family
Length = 732
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/82 (24%), Positives = 37/82 (45%)
Frame = -3
Query: 423 NYKFITKYNSILGKTISKYQGGAMPMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIH 244
++++ N+ + IS + + LK I ++ +I+DK+ LF+ L + DV EK +
Sbjct: 364 SFEYFINLNARSPEFISLFVDDKLRKGLKGITDVDVEVILDKVMMLFRYLQEKDVFEKYY 423
Query: 243 FHTPNSNTLFDYVHKDDLPKDL 178
L DD + L
Sbjct: 424 KQHLAKRLLSGKTVSDDAERSL 445
>At1g69670.1 68414.m08018 cullin, putative contains similarity to
Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from
[Homo sapiens]; contains Pfam profile PF00888: Cullin
family
Length = 732
Score = 29.5 bits (63), Expect = 2.5
Identities = 20/82 (24%), Positives = 37/82 (45%)
Frame = -3
Query: 423 NYKFITKYNSILGKTISKYQGGAMPMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIH 244
++++ N+ + IS + + LK + + LI+DK+ LF+ L + DV EK +
Sbjct: 364 SFEYFVNLNTRSPEFISLFVDDKLRKGLKGVGEEDVDLILDKVMMLFRYLQEKDVFEKYY 423
Query: 243 FHTPNSNTLFDYVHKDDLPKDL 178
L DD ++L
Sbjct: 424 KQHLAKRLLSGKTVSDDAERNL 445
>At1g22180.3 68414.m02773 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 249
Score = 29.5 bits (63), Expect = 2.5
Identities = 22/80 (27%), Positives = 29/80 (36%), Gaps = 4/80 (5%)
Frame = -3
Query: 351 PMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIHFHTPNSN----TLFDYVHKDDLPK 184
P RL V N P I + Y + KP L+ K+ F + N L D + L
Sbjct: 113 PERLGLAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEV 172
Query: 183 DLGGIRDSLDIYTKKMKEMM 124
GG +K E M
Sbjct: 173 AFGGKNSDAGFNFEKYAERM 192
>At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 314
Score = 29.5 bits (63), Expect = 2.5
Identities = 22/80 (27%), Positives = 29/80 (36%), Gaps = 4/80 (5%)
Frame = -3
Query: 351 PMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIHFHTPNSN----TLFDYVHKDDLPK 184
P RL V N P I + Y + KP L+ K+ F + N L D + L
Sbjct: 178 PERLGLAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEV 237
Query: 183 DLGGIRDSLDIYTKKMKEMM 124
GG +K E M
Sbjct: 238 AFGGKNSDAGFNFEKYAERM 257
>At1g22180.1 68414.m02772 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 249
Score = 29.5 bits (63), Expect = 2.5
Identities = 22/80 (27%), Positives = 29/80 (36%), Gaps = 4/80 (5%)
Frame = -3
Query: 351 PMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIHFHTPNSN----TLFDYVHKDDLPK 184
P RL V N P I + Y + KP L+ K+ F + N L D + L
Sbjct: 113 PERLGLAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEV 172
Query: 183 DLGGIRDSLDIYTKKMKEMM 124
GG +K E M
Sbjct: 173 AFGGKNSDAGFNFEKYAERM 192
>At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar to SEC14
CYTOSOLIC FACTOR (PHOSPHATIDYLINOSITOL/
PHOSPHATIDYLCHOLINE TRANSFER PROTEIN) GB:P46250 from
[Candida albicans] (Yeast (1996) 12(11), 1097-1105);
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 579
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 1/70 (1%)
Frame = -3
Query: 378 ISKYQGGAMPMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIH-FHTPNSNTLFDYVH 202
+ K G P L Q+++IN +++ K L KIH + L + +
Sbjct: 244 MQKIDGDNYPETLNQMYIINAGNGFKLVWNTVKGFLDPKTTSKIHVLGNKYRSHLLEIID 303
Query: 201 KDDLPKDLGG 172
+LP+ LGG
Sbjct: 304 PSELPEFLGG 313
>At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) /
phosphoglyceride transfer protein similar to
polyphosphoinositide binding protein SEC14 homolog Ssh1p
(GB:AAB94598) [Glycine max]; identified in Eur J Biochem
1998 Dec 1;258(2):402-10 as AtSEC14, characterized by
functional complementation in S. cerevisiae.
Length = 325
Score = 29.1 bits (62), Expect = 3.4
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -3
Query: 351 PMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKI 247
P + + +V+N P I + KPLL+ K+KI
Sbjct: 184 PEKTETYYVVNVPYIFSACWKTIKPLLQERTKKKI 218
>At4g11080.1 68417.m01800 high mobility group (HMG1/2) family
protein similar to SP|P40618 High mobility group protein
HMG2A {Gallus gallus}; contains Pfam profile PF00505:
HMG (high mobility group) box
Length = 446
Score = 28.7 bits (61), Expect = 4.4
Identities = 12/56 (21%), Positives = 30/56 (53%)
Frame = -2
Query: 265 RRKRKDTFSHAKFEHTIRLRTQRRLTKGLGRDQRQSGYLYKKNERNDANETRRPSS 98
++K ++ F + ++L ++ T + + +++ KKNE D N+ ++P+S
Sbjct: 322 QKKEEEEFMKLHKQEALQLLKKKEKTDNIIKKTKETAKNKKKNENVDPNKPKKPTS 377
>At3g50950.2 68416.m05579 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 852
Score = 28.7 bits (61), Expect = 4.4
Identities = 10/32 (31%), Positives = 20/32 (62%)
Frame = -3
Query: 171 IRDSLDIYTKKMKEMMLTKRDVLARDDLWRKN 76
+ D + +K+++ +L KR ++ DD+W KN
Sbjct: 242 VGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKN 273
>At3g50950.1 68416.m05578 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 852
Score = 28.7 bits (61), Expect = 4.4
Identities = 10/32 (31%), Positives = 20/32 (62%)
Frame = -3
Query: 171 IRDSLDIYTKKMKEMMLTKRDVLARDDLWRKN 76
+ D + +K+++ +L KR ++ DD+W KN
Sbjct: 242 VGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKN 273
>At4g36490.1 68417.m05181 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus; supporting cDNA gi|23463078|gb|BT000834.1|
Length = 543
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/70 (24%), Positives = 30/70 (42%), Gaps = 1/70 (1%)
Frame = -3
Query: 378 ISKYQGGAMPMRLKQIHVINTPLIVDKIYSLFKPLLKSDVKEKIH-FHTPNSNTLFDYVH 202
+ K G P L ++ +IN +++ K L KIH + L + +
Sbjct: 219 LQKVDGDNYPETLNRMFIINAGSGFRMLWNTVKSFLDPKTTAKIHVLGNKYQSKLLEIID 278
Query: 201 KDDLPKDLGG 172
+ +LP+ LGG
Sbjct: 279 ESELPEFLGG 288
>At1g67140.1 68414.m07638 expressed protein
Length = 2158
Score = 28.3 bits (60), Expect = 5.9
Identities = 13/50 (26%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = +2
Query: 263 SLLRRGLNNEYILSTISGVFM-TCICFKRIGIAPP-WYFDIVLPRIELYL 406
S++++ + + ST+ + C C+ IGI+P YF +++P + +L
Sbjct: 1444 SVVQQLVGTKIYYSTVGEIISYPCFCYPAIGISPDIMYFMVIIPLFDEFL 1493
>At2g38720.1 68415.m04755 microtubule associated protein
(MAP65/ASE1) family protein low similarity to myosin
[Schistosoma japonicum] GI:3941320; contains Pfam
profile PF03999: Microtubule associated protein
(MAP65/ASE1 family)
Length = 587
Score = 27.9 bits (59), Expect = 7.7
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -3
Query: 183 DLGGIRDSLDIYTKKMKEMMLTKRDVLARDDLWR 82
DL I S+D +K +E L+++++L + D WR
Sbjct: 348 DLSDILASIDGQIEKAREEALSRKEILDKVDKWR 381
>At1g48820.1 68414.m05463 terpene synthase/cyclase family protein
similar to terpene cyclase GI:9293912 from [Arabidopsis
thaliana]
Length = 561
Score = 27.9 bits (59), Expect = 7.7
Identities = 17/59 (28%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
Frame = +1
Query: 574 FFIRRFINCHFHNLHSF---LYRRR*WISISEKLRCFISDTNKKF*STLGXIQVIVXTM 741
+F R + CHF +L F R R ++S ++ FI DT ++ ++LG ++ + T+
Sbjct: 292 YFRDRLVECHFASLTCFEPKYARARIFLSKIFTVQIFIDDTCDRY-ASLGEVESLADTI 349
>At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24280) [Saccharomyces
cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700
come from this gene
Length = 540
Score = 27.9 bits (59), Expect = 7.7
Identities = 18/72 (25%), Positives = 30/72 (41%), Gaps = 4/72 (5%)
Frame = -3
Query: 324 INTPLIVDKIYSLFKPLLKSDVKEKIHFHTPNS--NTLFDYVHKDDLPKDLGGIR--DSL 157
IN P + ++ P L K K P TL Y+ D+LP GG + D
Sbjct: 370 INVPFWFYAMRAVLSPFLTQRTKSKFVVARPAKVRETLLKYIPADELPVQYGGFKTVDDT 429
Query: 156 DIYTKKMKEMML 121
+ + + E+++
Sbjct: 430 EFSNETVSEVVV 441
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,995,512
Number of Sequences: 28952
Number of extensions: 298106
Number of successful extensions: 978
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 946
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 977
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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