BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10b11f
(604 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 34 0.014
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 30 0.23
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.40
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 28 0.91
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 28 0.91
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 28 1.2
SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce... 27 1.6
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 27 2.1
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 26 3.7
SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 26 3.7
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 26 3.7
SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom... 26 3.7
SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||... 26 4.9
SPBC3B9.05 |||helper of TIM |Schizosaccharomyces pombe|chr 2|||M... 25 6.4
SPBC1289.14 ||SPBC8E4.10c|adducin|Schizosaccharomyces pombe|chr ... 25 6.4
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 25 8.5
SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb... 25 8.5
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 25 8.5
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 25 8.5
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 34.3 bits (75), Expect = 0.014
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 407 INLPLFLECGHTVCENCIRNIVKFHEPIECRICQ 508
I P +CGHT C CI + +K + C IC+
Sbjct: 205 ITTPYTTDCGHTYCYACIMSRLKLVNNVSCPICK 238
>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 30.3 bits (65), Expect = 0.23
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 416 PLFLECGHTVCENCIRNIVKFHEPIECRICQ 508
PL C HT C CIR+ ++ H P+ C C+
Sbjct: 39 PLITSCSHTFCSFCIRDYLREH-PM-CPACR 67
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 29.5 bits (63), Expect = 0.40
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +2
Query: 416 PLFLECGHTVCENCIRNIVKFHEPIECRICQKCMEIDSSESCLL 547
P CGHT C C+ N +K E C C++ + S + L+
Sbjct: 95 PFTTHCGHTYCYECLLNWLK--ESKSCPTCRQKLYTQPSPAYLV 136
>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
subunit |Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 28.3 bits (60), Expect = 0.91
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +2
Query: 455 CIRNIVKFHEPIECRICQKCMEID 526
C+R + EP++C +C +C+E D
Sbjct: 169 CLRKTIS--EPVKCNLCYECIESD 190
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 28.3 bits (60), Expect = 0.91
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +2
Query: 416 PLFLECGHTVCENCIRN 466
P+ L CG TVC C RN
Sbjct: 29 PVTLPCGGTVCRKCFRN 45
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 27.9 bits (59), Expect = 1.2
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 407 INLPLFLECGHTVCENCIRNIVKFHEPIECRICQ 508
I+ P ECGH C +CI + EC +C+
Sbjct: 263 IHCPAATECGHIFCWSCINGWTS--KKSECPLCR 294
>SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1297
Score = 27.5 bits (58), Expect = 1.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 94 FFNDILRFHTLLKPEIAIHFFLIGQCA 174
F N +LRF+ LK ++ +HF L C+
Sbjct: 783 FTNILLRFYLCLKDDVDMHFLLNMDCS 809
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 27.1 bits (57), Expect = 2.1
Identities = 21/116 (18%), Positives = 45/116 (38%), Gaps = 5/116 (4%)
Frame = +2
Query: 179 PLVFVENTIQTFLFSQIKHKEIDLLTIRKMFPLK*SKVIAKIFKVVKMEPIDLETKKCCP 358
P++ TI + F + ++ F + + + I K+ ++ + +C
Sbjct: 820 PVLLSNMTINSETFDDFEFSVEQFNSLINQFVVTGKPIPSDILKIDTLKSFEALITECPI 879
Query: 359 NCSQPYQFQILSTISKINLPLFLECGHTVCENCIRNIVKFHE-----PIECRICQK 511
C++P Q PL L C H C +C+ +++ + P C C++
Sbjct: 880 CCNEPIQN-----------PLLLNCKHACCGDCLSEHIQYQKRRNIIPPLCHTCRQ 924
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 26.2 bits (55), Expect = 3.7
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = +2
Query: 419 LFLECGHTVCENCIRNIVKFHEPIECRICQ 508
L CGH C NC+ + H+ C C+
Sbjct: 651 LIPNCGHAFCSNCMEPFYE-HKTSTCPQCE 679
>SPAC688.11 |end4|sla2|Huntingtin-interacting protein
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1092
Score = 26.2 bits (55), Expect = 3.7
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +2
Query: 215 LFSQIKHKEIDLLTIRKMFPLK*SKVIAKIFKVVKME 325
L++Q++ + +DLL+ K LK S I K KME
Sbjct: 487 LYTQLRQEHLDLLSKYKQIQLKASSAQEAIDKKEKME 523
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 26.2 bits (55), Expect = 3.7
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = +2
Query: 428 ECGHTVCENCIRNIVKFHEPIECRICQK 511
+CGH C C + + K P + C+K
Sbjct: 166 KCGHLFCSTCAKELRKKTVPCPVQHCRK 193
>SPCC1235.10c |sec6||exocyst complex subunit
Sec6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 730
Score = 26.2 bits (55), Expect = 3.7
Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Frame = +3
Query: 366 ANLINFKYFQLYQRSTYHCSWNVVTLFVKIV-LETLSSFTNRLNAGYARNVWKLIHLNHV 542
+ L F+ LYQ S S +TL L TLS +RL + RN+++L+ H+
Sbjct: 130 SKLREFREEALYQASLEGQSDLPITLENSFSNLNTLSDNFDRLVLNFCRNIFQLVKSGHI 189
>SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr
2|||Manual
Length = 525
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +2
Query: 380 FQILSTISKINLPLFLECGHTVCENCI 460
F +LS LPLFLE V + CI
Sbjct: 443 FPLLSNCLLEELPLFLEMARNVTDECI 469
>SPBC3B9.05 |||helper of TIM |Schizosaccharomyces pombe|chr
2|||Manual
Length = 116
Score = 25.4 bits (53), Expect = 6.4
Identities = 11/45 (24%), Positives = 22/45 (48%)
Frame = +2
Query: 287 KVIAKIFKVVKMEPIDLETKKCCPNCSQPYQFQILSTISKINLPL 421
K K F++ + +D E+ + CPNC + ++ + K P+
Sbjct: 46 KKCRKAFRIQFDQTMD-ESDEYCPNCDNHFVIDAITKVEKPKAPI 89
>SPBC1289.14 ||SPBC8E4.10c|adducin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 324
Score = 25.4 bits (53), Expect = 6.4
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -3
Query: 545 VDMIQMNQFPYISGISCIQ 489
V I N +PY +G+SC++
Sbjct: 171 VKSIMHNHYPYAAGVSCVK 189
>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 536
Score = 25.0 bits (52), Expect = 8.5
Identities = 9/28 (32%), Positives = 11/28 (39%)
Frame = +2
Query: 431 CGHTVCENCIRNIVKFHEPIECRICQKC 514
CG +C C+ I P IC C
Sbjct: 300 CGRVICRFCVLEISLPQHPQPLLICMSC 327
>SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 25.0 bits (52), Expect = 8.5
Identities = 6/16 (37%), Positives = 12/16 (75%)
Frame = +2
Query: 443 VCENCIRNIVKFHEPI 490
+C+ C+ I+ F+EP+
Sbjct: 346 ICDQCLEAIIDFYEPV 361
>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 677
Score = 25.0 bits (52), Expect = 8.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +2
Query: 416 PLFLECGHTVCENCIRNIVKFHEPIECRICQK 511
P L CGH + +C+RN ++ + C IC++
Sbjct: 323 PKRLPCGHILHFHCLRNWLERQQ--TCPICRR 352
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 25.0 bits (52), Expect = 8.5
Identities = 8/28 (28%), Positives = 13/28 (46%)
Frame = +2
Query: 425 LECGHTVCENCIRNIVKFHEPIECRICQ 508
L C H +C C + ++ EC C+
Sbjct: 94 LPCNHRMCHVCALRLRALYKTKECTFCK 121
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,511,827
Number of Sequences: 5004
Number of extensions: 51789
Number of successful extensions: 151
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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