SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10b11f
         (604 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    34   0.014
SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb...    30   0.23 
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    29   0.40 
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su...    28   0.91 
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    28   0.91 
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi...    28   1.2  
SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce...    27   1.6  
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    27   2.1  
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B...    26   3.7  
SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch...    26   3.7  
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar...    26   3.7  
SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom...    26   3.7  
SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||...    26   4.9  
SPBC3B9.05 |||helper of TIM |Schizosaccharomyces pombe|chr 2|||M...    25   6.4  
SPBC1289.14 ||SPBC8E4.10c|adducin|Schizosaccharomyces pombe|chr ...    25   6.4  
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo...    25   8.5  
SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb...    25   8.5  
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch...    25   8.5  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    25   8.5  

>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 251

 Score = 34.3 bits (75), Expect = 0.014
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +2

Query: 407 INLPLFLECGHTVCENCIRNIVKFHEPIECRICQ 508
           I  P   +CGHT C  CI + +K    + C IC+
Sbjct: 205 ITTPYTTDCGHTYCYACIMSRLKLVNNVSCPICK 238


>SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 387

 Score = 30.3 bits (65), Expect = 0.23
 Identities = 13/31 (41%), Positives = 18/31 (58%)
 Frame = +2

Query: 416 PLFLECGHTVCENCIRNIVKFHEPIECRICQ 508
           PL   C HT C  CIR+ ++ H P+ C  C+
Sbjct: 39  PLITSCSHTFCSFCIRDYLREH-PM-CPACR 67


>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 468

 Score = 29.5 bits (63), Expect = 0.40
 Identities = 14/44 (31%), Positives = 21/44 (47%)
 Frame = +2

Query: 416 PLFLECGHTVCENCIRNIVKFHEPIECRICQKCMEIDSSESCLL 547
           P    CGHT C  C+ N +K  E   C  C++ +    S + L+
Sbjct: 95  PFTTHCGHTYCYECLLNWLK--ESKSCPTCRQKLYTQPSPAYLV 136


>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
           subunit |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 271

 Score = 28.3 bits (60), Expect = 0.91
 Identities = 9/24 (37%), Positives = 16/24 (66%)
 Frame = +2

Query: 455 CIRNIVKFHEPIECRICQKCMEID 526
           C+R  +   EP++C +C +C+E D
Sbjct: 169 CLRKTIS--EPVKCNLCYECIESD 190


>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 486

 Score = 28.3 bits (60), Expect = 0.91
 Identities = 10/17 (58%), Positives = 11/17 (64%)
 Frame = +2

Query: 416 PLFLECGHTVCENCIRN 466
           P+ L CG TVC  C RN
Sbjct: 29  PVTLPCGGTVCRKCFRN 45


>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 306

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 12/34 (35%), Positives = 17/34 (50%)
 Frame = +2

Query: 407 INLPLFLECGHTVCENCIRNIVKFHEPIECRICQ 508
           I+ P   ECGH  C +CI       +  EC +C+
Sbjct: 263 IHCPAATECGHIFCWSCINGWTS--KKSECPLCR 294


>SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1297

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = +1

Query: 94  FFNDILRFHTLLKPEIAIHFFLIGQCA 174
           F N +LRF+  LK ++ +HF L   C+
Sbjct: 783 FTNILLRFYLCLKDDVDMHFLLNMDCS 809


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 21/116 (18%), Positives = 45/116 (38%), Gaps = 5/116 (4%)
 Frame = +2

Query: 179  PLVFVENTIQTFLFSQIKHKEIDLLTIRKMFPLK*SKVIAKIFKVVKMEPIDLETKKCCP 358
            P++    TI +  F   +       ++   F +    + + I K+  ++  +    +C  
Sbjct: 820  PVLLSNMTINSETFDDFEFSVEQFNSLINQFVVTGKPIPSDILKIDTLKSFEALITECPI 879

Query: 359  NCSQPYQFQILSTISKINLPLFLECGHTVCENCIRNIVKFHE-----PIECRICQK 511
             C++P Q            PL L C H  C +C+   +++ +     P  C  C++
Sbjct: 880  CCNEPIQN-----------PLLLNCKHACCGDCLSEHIQYQKRRNIIPPLCHTCRQ 924


>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
           Brl1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 692

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 10/30 (33%), Positives = 14/30 (46%)
 Frame = +2

Query: 419 LFLECGHTVCENCIRNIVKFHEPIECRICQ 508
           L   CGH  C NC+    + H+   C  C+
Sbjct: 651 LIPNCGHAFCSNCMEPFYE-HKTSTCPQCE 679


>SPAC688.11 |end4|sla2|Huntingtin-interacting protein
           homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1092

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = +2

Query: 215 LFSQIKHKEIDLLTIRKMFPLK*SKVIAKIFKVVKME 325
           L++Q++ + +DLL+  K   LK S     I K  KME
Sbjct: 487 LYTQLRQEHLDLLSKYKQIQLKASSAQEAIDKKEKME 523


>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
           Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 205

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 9/28 (32%), Positives = 14/28 (50%)
 Frame = +2

Query: 428 ECGHTVCENCIRNIVKFHEPIECRICQK 511
           +CGH  C  C + + K   P   + C+K
Sbjct: 166 KCGHLFCSTCAKELRKKTVPCPVQHCRK 193


>SPCC1235.10c |sec6||exocyst complex subunit
           Sec6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 730

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
 Frame = +3

Query: 366 ANLINFKYFQLYQRSTYHCSWNVVTLFVKIV-LETLSSFTNRLNAGYARNVWKLIHLNHV 542
           + L  F+   LYQ S    S   +TL      L TLS   +RL   + RN+++L+   H+
Sbjct: 130 SKLREFREEALYQASLEGQSDLPITLENSFSNLNTLSDNFDRLVLNFCRNIFQLVKSGHI 189


>SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 525

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 12/27 (44%), Positives = 14/27 (51%)
 Frame = +2

Query: 380 FQILSTISKINLPLFLECGHTVCENCI 460
           F +LS      LPLFLE    V + CI
Sbjct: 443 FPLLSNCLLEELPLFLEMARNVTDECI 469


>SPBC3B9.05 |||helper of TIM |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 116

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 11/45 (24%), Positives = 22/45 (48%)
 Frame = +2

Query: 287 KVIAKIFKVVKMEPIDLETKKCCPNCSQPYQFQILSTISKINLPL 421
           K   K F++   + +D E+ + CPNC   +    ++ + K   P+
Sbjct: 46  KKCRKAFRIQFDQTMD-ESDEYCPNCDNHFVIDAITKVEKPKAPI 89


>SPBC1289.14 ||SPBC8E4.10c|adducin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 324

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 8/19 (42%), Positives = 13/19 (68%)
 Frame = -3

Query: 545 VDMIQMNQFPYISGISCIQ 489
           V  I  N +PY +G+SC++
Sbjct: 171 VKSIMHNHYPYAAGVSCVK 189


>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
           component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 536

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 9/28 (32%), Positives = 11/28 (39%)
 Frame = +2

Query: 431 CGHTVCENCIRNIVKFHEPIECRICQKC 514
           CG  +C  C+  I     P    IC  C
Sbjct: 300 CGRVICRFCVLEISLPQHPQPLLICMSC 327


>SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 431

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 6/16 (37%), Positives = 12/16 (75%)
 Frame = +2

Query: 443 VCENCIRNIVKFHEPI 490
           +C+ C+  I+ F+EP+
Sbjct: 346 ICDQCLEAIIDFYEPV 361


>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 677

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = +2

Query: 416 PLFLECGHTVCENCIRNIVKFHEPIECRICQK 511
           P  L CGH +  +C+RN ++  +   C IC++
Sbjct: 323 PKRLPCGHILHFHCLRNWLERQQ--TCPICRR 352


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 8/28 (28%), Positives = 13/28 (46%)
 Frame = +2

Query: 425 LECGHTVCENCIRNIVKFHEPIECRICQ 508
           L C H +C  C   +   ++  EC  C+
Sbjct: 94  LPCNHRMCHVCALRLRALYKTKECTFCK 121


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,511,827
Number of Sequences: 5004
Number of extensions: 51789
Number of successful extensions: 151
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -