BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10a07f (609 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g45140.1 68418.m05542 DNA-directed RNA polymerase, putative s... 27 9.7 At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 27 9.7 At3g50710.1 68416.m05548 F-box family protein contains F-box dom... 27 9.7 At3g01780.1 68416.m00118 expressed protein est hit, 27 9.7 At1g05490.1 68414.m00559 C protein immunoglobulin-A-binding beta... 27 9.7 >At5g45140.1 68418.m05542 DNA-directed RNA polymerase, putative similar to SP|P22276 DNA-directed RNA polymerase III 130 kDa polypeptide (EC 2.7.7.6) (RNA polymerase III subunit 2) {Saccharomyces cerevisiae}; contains Pfam profiles PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567: RNA polymerase Rpb2 domain 5 Length = 1150 Score = 27.1 bits (57), Expect = 9.7 Identities = 10/34 (29%), Positives = 23/34 (67%) Frame = -3 Query: 226 LSSAVSPDLILSKSSSLKPVNCDMFYLFLHRYVR 125 ++++V+ ++KS ++ + + YLFLHR+V+ Sbjct: 208 INASVTSSTEMTKSKTVIQMEKEKIYLFLHRFVK 241 >At4g30160.1 68417.m04289 villin, putative similar to villin 2 (VLN2) [Arabidopsis thaliana] GI:3415115, villin 3 (VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam profiles PF00626: Gelsolin repeat, PF02209: Villin headpiece domain Length = 974 Score = 27.1 bits (57), Expect = 9.7 Identities = 25/87 (28%), Positives = 42/87 (48%), Gaps = 10/87 (11%) Frame = +3 Query: 366 KLSLKTPTSPS--KNAKMEEDKQNQVILQNNVEGNDE---VQVDTIEA--DKSDMTS--- 515 K S KTP SP+ N+K +E+K+ + ++ E +Q D E D+ D+ + Sbjct: 858 KASPKTPESPAPESNSKEQEEKKENDKEEGSMSSRIESLTIQEDAKEGVEDEEDLPAHPY 917 Query: 516 SRRSSTAPDIVRGVDFQYTEHELASHK 596 R +T+ D V +D E L+S + Sbjct: 918 DRLKTTSTDPVSDIDVTRREAYLSSEE 944 >At3g50710.1 68416.m05548 F-box family protein contains F-box domain Pfam:PF00646 Length = 427 Score = 27.1 bits (57), Expect = 9.7 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -2 Query: 317 LINGCLIFIWLGFH**FNSFYIRIFYVLVFTL 222 L++GC + L +NSF++R FYV + TL Sbjct: 175 LLSGCPVLEELVLDKSYNSFHVRSFYVEIPTL 206 >At3g01780.1 68416.m00118 expressed protein est hit, Length = 1176 Score = 27.1 bits (57), Expect = 9.7 Identities = 15/72 (20%), Positives = 32/72 (44%), Gaps = 1/72 (1%) Frame = +3 Query: 366 KLSLKTPTSPSKNAKMEEDKQNQVILQNNVEGNDEVQVDTIEADK-SDMTSSRRSSTAPD 542 +++L P K +K+EE+ +N+ + E +DE + E ++ D ++ Sbjct: 1081 RIALLKAAQPKKTSKIEEESENEEEEEGEEEDDDEEVKEKKEKEEGKDKEEKKKKEKEKG 1140 Query: 543 IVRGVDFQYTEH 578 + + TEH Sbjct: 1141 TFSKLTAEETEH 1152 >At1g05490.1 68414.m00559 C protein immunoglobulin-A-binding beta antigen-related contains weak similarity to C protein immunoglobulin-A-binding beta antigen [Streptococcus agalactiae] gi|18028989|gb|AAL56250 Length = 731 Score = 27.1 bits (57), Expect = 9.7 Identities = 16/61 (26%), Positives = 29/61 (47%) Frame = +3 Query: 414 EEDKQNQVILQNNVEGNDEVQVDTIEADKSDMTSSRRSSTAPDIVRGVDFQYTEHELASH 593 EE+++NQ NN + N V+ + E S +SS SS++ D Y + + + Sbjct: 256 EEEEENQDSEDNNTKDNVTVESLSSEDPSSSSSSSSSSSSSSSSSSSDDESYVKEVVGDN 315 Query: 594 K 596 + Sbjct: 316 R 316 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,815,075 Number of Sequences: 28952 Number of extensions: 227901 Number of successful extensions: 539 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 523 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 539 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1216725696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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