BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10a02f
(544 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12260.1 68416.m01531 complex 1 family protein / LVR family p... 58 3e-09
At2g47220.1 68415.m05897 3' exoribonuclease family domain 1 prot... 29 2.0
At4g32300.1 68417.m04596 lectin protein kinase family protein co... 28 4.6
At1g13350.1 68414.m01550 protein kinase family protein contains ... 28 4.6
At5g22400.1 68418.m02613 rac GTPase activating protein, putative... 27 6.1
At2g18760.1 68415.m02184 SNF2 domain-containing protein / helica... 27 6.1
At3g25840.1 68416.m03219 protein kinase family protein contains ... 27 8.1
>At3g12260.1 68416.m01531 complex 1 family protein / LVR family
protein contains Pfam PF05347: Complex 1 protein (LYR
family)
Length = 133
Score = 58.4 bits (135), Expect = 3e-09
Identities = 32/91 (35%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
Frame = +1
Query: 121 PVLSSSHAEARNRVLSLYKAWYRQIPYIVKDYDIPK--SEAQCREKLKELFIKNKHVTDI 294
P S++ EAR RV ++A R IP I+ Y++ + +Q R + N H+TD
Sbjct: 13 PPNSANLTEARRRVFDFFRAACRSIPTIMDIYNLQDVVAPSQLRYAISAQIRNNAHITDP 72
Query: 295 RVIDMLVIKGQMELKESVNIWKQKGHIMAYF 387
+VID+L+ KG EL + V+ KQ+ HI+ +
Sbjct: 73 KVIDLLIFKGMEELTDIVDHAKQRHHIIGQY 103
>At2g47220.1 68415.m05897 3' exoribonuclease family domain 1
protein-related similar to polynucleotide phosphorylase
[Pisum sativum] GI:2286200, polyribonucleotide
phophorylase [Spinacia oleracea] GI:1924972; contains
Pfam profiles PF05266: Protein of unknown function
(DUF724), weak hit to PF01138: 3' exoribonuclease
family, domain 1
Length = 469
Score = 29.1 bits (62), Expect = 2.0
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = +1
Query: 271 KNKHVTDIRVIDMLVIKGQMELKESVNIWKQKGHIMAYFKPTEEPKPKNFLSKFFSG 441
K+ VT + + I GQ ELK +N WK G +++ + K +S FSG
Sbjct: 100 KDHEVTFLCLPAASYITGQEELKACLNDWKSNGLVVSGLVRDQREKLIQEVSSTFSG 156
>At4g32300.1 68417.m04596 lectin protein kinase family protein
contains Pfam domains, PF01453: Lectin (probable mannose
binding) and PF00069: Protein kinase domain
Length = 821
Score = 27.9 bits (59), Expect = 4.6
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 18 LFSLKDFCVGSSHRLIHFENVCKAGNKSWYKNRK 119
L L+ FC +HRL+ +E + K + W +K
Sbjct: 548 LVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRKK 581
>At1g13350.1 68414.m01550 protein kinase family protein contains
protein kinase domain, Pfam:PF00069 (likely that this
cDNA contains a single unspliced intron. Putative intron
removed in this gene model.)
Length = 761
Score = 27.9 bits (59), Expect = 4.6
Identities = 26/101 (25%), Positives = 44/101 (43%), Gaps = 2/101 (1%)
Frame = +1
Query: 151 RNRVLSLYKAWYRQIPYIVKDY--DIPKSEAQCREKLKELFIKNKHVTDIRVIDMLVIKG 324
RN + ++++ + + IVK Y +I + R +LFI KH+ + V+ +
Sbjct: 518 RNHLCLVFESLHLNLREIVKKYGRNIGIQLSGVRVYATQLFISLKHLKNCGVLHCDIKPD 577
Query: 325 QMELKESVNIWKQKGHIMAYFKPTEEPKPKNFLSKFFSGNE 447
M + E N K A F T E P +S+F+ E
Sbjct: 578 NMLVNEGRNTLKLCDFGSAMFAGTNEVTP-YLVSRFYRAPE 617
>At5g22400.1 68418.m02613 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 [Lotus
japonicus] GI:3695059; contains Pfam profile PF00620:
RhoGAP domain
Length = 466
Score = 27.5 bits (58), Expect = 6.1
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 169 LYKAWYRQIPYIVKDYDIPKSEAQCR 246
L KAW+R++P V D P+ QC+
Sbjct: 234 LIKAWFRELPTSVLDSLSPEQVMQCQ 259
>At2g18760.1 68415.m02184 SNF2 domain-containing protein / helicase
domain-containing protein similar to SP|Q03468 Excision
repair protein ERCC-6 (Cockayne syndrome protein CSB)
{Homo sapiens}; contains PFam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain
Length = 1187
Score = 27.5 bits (58), Expect = 6.1
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +1
Query: 322 GQMEL-KESVNIWKQKGHIMAYFKPTEEPKPKNFLSKFFSGNE 447
G+M++ E + +WKQ+GH + F T++ + L F NE
Sbjct: 727 GKMKVVAEVLKVWKQQGHRVLLFSQTQQ--MLDILESFLVANE 767
>At3g25840.1 68416.m03219 protein kinase family protein contains Pfam
profile: PF00069 eukaryotic protein kinase domain
Length = 935
Score = 27.1 bits (57), Expect = 8.1
Identities = 27/110 (24%), Positives = 49/110 (44%), Gaps = 2/110 (1%)
Frame = +1
Query: 151 RNRVLSLYKAWYRQIPYIVKDY--DIPKSEAQCREKLKELFIKNKHVTDIRVIDMLVIKG 324
RN + ++++ + + ++K + +I + R K+LFI KH+ + V+ +
Sbjct: 693 RNHLCLVFESLHLNLREVLKKFGRNIGLQLSAVRAYSKQLFIALKHLKNCGVLHCDIKPD 752
Query: 325 QMELKESVNIWKQKGHIMAYFKPTEEPKPKNFLSKFFSGNE*FIYLLCTY 474
M + E N+ K A F E P +S+F+ E I L TY
Sbjct: 753 NMLVNEGKNVLKLCDFGNAMFAGKNEVTP-YLVSRFYRSPE--IILGLTY 799
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,953,167
Number of Sequences: 28952
Number of extensions: 218735
Number of successful extensions: 608
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 599
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 608
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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