Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P07_F_F22
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo... 29 0.77
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 27 1.8
SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 27 2.4
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 27 3.1
SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 26 4.1
SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces p... 26 5.5
SPAC3H8.06 |aur1||inositol phosphorylceramide synthase |Schizosa... 26 5.5
SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 26 5.5
SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 25 7.2
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 25 9.5
SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 25 9.5
SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces pom... 25 9.5
>SPAC3C7.07c |||arginine-tRNA protein transferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 361
Score = 28.7 bits (61), Expect = 0.77
Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 3/40 (7%)
Frame = -2
Query: 111 GAI*YTTRVE-CTYQDEKLEIITSNNM--NLSKEHNXKKK 1
GA Y+ +E CTY DEK E+ M +L KE KK
Sbjct: 93 GAEKYSVTMEPCTYTDEKFEVFKKYQMQVHLEKEEEITKK 132
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 27.5 bits (58), Expect = 1.8
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +3
Query: 303 KSYTTRLKEDVKSMLENFEEIIKLAKVENESQLNRMTQIE 422
K+YT + KED +L E I K ENE LN T +E
Sbjct: 98 KAYTAKTKEDFAYLLSQLETIFK----ENEI-LNEFTPVE 132
>SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1717
Score = 27.1 bits (57), Expect = 2.4
Identities = 19/60 (31%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = +1
Query: 478 SLMKLVSDIKQYL-ILNDFPSVNEAITQNSKLFRTKQQECDQKLMSLRDDIAADLYDLED 654
SL KL+ DI L DFPS++ T E D L + D + + YD E+
Sbjct: 354 SLWKLILDIAPDAGDLFDFPSLSSISKDLCVPIETPVSEIDISLTAYNDPVISPYYDTEE 413
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 26.6 bits (56), Expect = 3.1
Identities = 19/67 (28%), Positives = 33/67 (49%)
Frame = +3
Query: 249 KEQTNMQRSLPQNKEALLKSYTTRLKEDVKSMLENFEEIIKLAKVENESQLNRMTQIEQD 428
+ Q +Q N E S K +++S E+F IK ENE +++R+ Q E+D
Sbjct: 273 QHQAELQ-DFASNIEQKANSLIMEYKNELQSAEEHFSHKIKELTSENELKISRL-QEEKD 330
Query: 429 TFEMQVR 449
+ +V+
Sbjct: 331 SLLKKVQ 337
>SPAC6C3.06c |||P-type ATPase, calcium
transporting|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1033
Score = 26.2 bits (55), Expect = 4.1
Identities = 9/25 (36%), Positives = 19/25 (76%)
Frame = -3
Query: 233 VAFNSFVSLFVIFSAVYNRDVTKQL 159
V +++ ++ +FS VY+RDV+++L
Sbjct: 863 VGYSTMYTMLPVFSIVYDRDVSEKL 887
>SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1033
Score = 25.8 bits (54), Expect = 5.5
Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Frame = +1
Query: 454 ANIVRAGESLMKLVSDIKQYLILNDFPSVNEAITQNSKL--FRTKQQECDQKLMSLRDD 624
A +V+ + +MK + +I+ I + S + + NSKL F QQ +++L D+
Sbjct: 896 AMLVKLSKKIMKCIDEIETKDIEEELGSNKKTESSNSKLPEFTPLQQSLEEELQEGADE 954
>SPAC3H8.06 |aur1||inositol phosphorylceramide synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 422
Score = 25.8 bits (54), Expect = 5.5
Identities = 11/33 (33%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +3
Query: 6 FFXYVLWISSC--YLMLLFLISHLGRYILLLLC 98
F+ YVLW+ C YL + + +G L ++C
Sbjct: 277 FYGYVLWLCWCTMYLTHHYFVDLVGGMCLAIIC 309
>SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 649
Score = 25.8 bits (54), Expect = 5.5
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +3
Query: 225 KSYASLSNKEQT-NMQRSLPQNKEALLKSYTTRLKEDVKSMLENFEEIIKLAKVENESQL 401
+S+A + +Q+ MQR L Q + +LK EDV L+N EE+ + + S L
Sbjct: 358 QSFADDDDLQQSIAMQRRLAQKRAKILKP------EDVAEQLQNAEEVTDMTDSDTASGL 411
>SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 25.4 bits (53), Expect = 7.2
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = -2
Query: 117 LCGAI*YTTRVECTYQDEKLEIITSNNMNLSKEHNXK 7
LC AI Y R C ++D KLE N+ L K +N K
Sbjct: 132 LCCAIQYLHRQGCVHRDLKLE-----NIFLDKAYNVK 163
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 25.0 bits (52), Expect = 9.5
Identities = 7/23 (30%), Positives = 16/23 (69%)
Frame = +1
Query: 76 GTFYSCCVLNCTTQLLILCTKSK 144
G+++ C VL+CT + ++ K++
Sbjct: 362 GSWFKCLVLDCTNRFVVFGNKTR 384
>SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -3
Query: 590 SCCFVRNNFEFWVMASLTDGKSLRIKYCLISDTSFISDS 474
SC ++ + + S+ DGK L I CL++ +S D+
Sbjct: 299 SCRQIKRSSVVSLCNSVFDGKKLTIAKCLLTSSSIKDDT 337
>SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 420
Score = 25.0 bits (52), Expect = 9.5
Identities = 25/100 (25%), Positives = 45/100 (45%)
Frame = +3
Query: 225 KSYASLSNKEQTNMQRSLPQNKEALLKSYTTRLKEDVKSMLENFEEIIKLAKVENESQLN 404
K S ++ +Q+S + KE +RL+ED++ L FEE + K +++
Sbjct: 136 KKLESRRQAYESLLQKSFKEKKE------DSRLEEDIRLALYKFEESTEQVK----NRMI 185
Query: 405 RMTQIEQDTFEMQVRGC*YSQSWRITYETSIRY*TVFNSQ 524
+ +E D ++ Y ++ T I T+FNSQ
Sbjct: 186 ALKDVEADQYQQLTELIVYELNF-FKESTGILN-TIFNSQ 223
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,374,155
Number of Sequences: 5004
Number of extensions: 44464
Number of successful extensions: 171
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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