Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P07_F_A08
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 198 6e-52
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 73 5e-14
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 52 7e-08
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 48 9e-07
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 40 4e-04
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 36 0.004
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 29 0.77
SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces pombe... 28 1.0
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 27 3.1
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 4.1
SPAC24B11.13 |hem3|SPAC806.01|hydroxymethylbilane synthase|Schiz... 26 4.1
SPBC4B4.01c |||fumble family pantothenate kinase |Schizosaccharo... 25 9.5
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 9.5
>SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 204
Score = 198 bits (483), Expect = 6e-52
Identities = 86/159 (54%), Positives = 123/159 (77%)
Frame = +2
Query: 125 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDT 304
MSI+ YNGG+ VAM G++CVAIA+D R G+Q+ +++ NFPKVF MG Y+GL GLATD
Sbjct: 1 MSIMEYNGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDV 60
Query: 305 QTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPY 484
QT+++ ++++NLY+ +E R ++PKTF+ ++S+ LYE+RFGPYF PV+AG+ DN P+
Sbjct: 61 QTLYELFRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVS-NDNTPF 119
Query: 485 VCNMDLIGCPNEPEDFVVSGTCSEQLYGMCEALWGAQLE 601
+C D IGC + EDF+VSGT +EQLYGMCE+++ LE
Sbjct: 120 ICGFDSIGCIDFAEDFIVSGTATEQLYGMCESVYEPNLE 158
Score = 42.3 bits (95), Expect = 6e-05
Identities = 18/22 (81%), Positives = 21/22 (95%)
Frame = +3
Query: 588 EPNLKPDELFETISQALVNAAD 653
EPNL+PD+LFETISQAL+NA D
Sbjct: 154 EPNLEPDDLFETISQALLNAQD 175
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 72.5 bits (170), Expect = 5e-14
Identities = 37/123 (30%), Positives = 66/123 (53%), Gaps = 1/123 (0%)
Frame = +2
Query: 143 NGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNF-PKVFQMGPTLYVGLPGLATDTQTVFQ 319
NGG VA+ G +A D R + ++T F P+V ++G L +G G D + +
Sbjct: 12 NGGTTVAIAGDGFAILAGDTR-SVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVK 70
Query: 320 RLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMD 499
R++ R++LY R M ++ + M+ LLY +RF PY++ +AG+D + + + + D
Sbjct: 71 RIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDK-EGKGEIYSFD 129
Query: 500 LIG 508
+G
Sbjct: 130 PVG 132
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 52.0 bits (119), Expect = 7e-08
Identities = 37/125 (29%), Positives = 62/125 (49%), Gaps = 4/125 (3%)
Frame = +2
Query: 146 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 325
G +V+A+K D V IA D + + ++ ++G VG G +D Q + QRL
Sbjct: 43 GSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQI-QRL 101
Query: 326 KFRMNLYE--LKENRMMRPKTFSAMLSNLLYERR--FGPYFIEPVIAGLDPYDNQPYVCN 493
++ + E + ++P LS +LY RR PY+ + ++AG+D + +PYV
Sbjct: 102 LEKLEIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENKEPYVAF 161
Query: 494 MDLIG 508
DL G
Sbjct: 162 ADLRG 166
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 48.4 bits (110), Expect = 9e-07
Identities = 35/145 (24%), Positives = 68/145 (46%)
Frame = +2
Query: 146 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 325
G + A++ +D V +A D R + A + K+ Q+ ++ G A DTQTV L
Sbjct: 24 GTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLL 83
Query: 326 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLI 505
K+ +++Y ++ T + + S + Y+ + ++AG D V ++ L
Sbjct: 84 KYYLSMYRIQFGHDPSVHTAATLASEMCYQNK-NMLSAGLIVAGYDEKTGGD-VYSIPLG 141
Query: 506 GCPNEPEDFVVSGTCSEQLYGMCEA 580
G ++ + + G+ S +YG C+A
Sbjct: 142 GSLHK-QPLAIGGSGSAFIYGFCDA 165
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 39.5 bits (88), Expect = 4e-04
Identities = 28/128 (21%), Positives = 58/128 (45%), Gaps = 4/128 (3%)
Frame = +2
Query: 146 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 325
G +A++G +C+ I +++ + Q VS NF K+ + + + GL D + + +
Sbjct: 30 GTTAIALRGNECIVIGVERKNVPKLQNVS-NFQKIAMVDNHVCLAFAGLNADARILIDKA 88
Query: 326 KFRMNLYELKENRMMRPKTFSAMLSNLL--YERRFG--PYFIEPVIAGLDPYDNQPYVCN 493
+ ++L + + + ++ + Y + G P+ + +IAG D DN P V
Sbjct: 89 RVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFDVGDNTPRVYQ 148
Query: 494 MDLIGCPN 517
+ G N
Sbjct: 149 TEPAGIYN 156
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 36.3 bits (80), Expect = 0.004
Identities = 23/90 (25%), Positives = 37/90 (41%)
Frame = +2
Query: 143 NGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQR 322
+G +A + Q + + D R S KV ++ P L L G A D Q
Sbjct: 60 HGTTTLAFRYQHGIVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETV 119
Query: 323 LKFRMNLYELKENRMMRPKTFSAMLSNLLY 412
L L++L+ ++ S +LSN+ Y
Sbjct: 120 LGMECRLHQLRNKELISVSAASKILSNITY 149
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 28.7 bits (61), Expect = 0.77
Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Frame = +2
Query: 149 GAVVAMKGQD-CVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLAT 298
GA+ +D VA A D G+ S + +VF++G L VG+ G T
Sbjct: 407 GALFKFDTEDEVVAWANDSPVGLAGYLFSKDISRVFRVGEALQVGMVGCNT 457
>SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 520
Score = 28.3 bits (60), Expect = 1.0
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +3
Query: 567 VCVKLSGEPNLKPDELFETISQALVNA 647
V LSG+P+LKP E+F+ A +N+
Sbjct: 153 VSQSLSGDPSLKPAEVFDRKQSAEINS 179
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 26.6 bits (56), Expect = 3.1
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = +2
Query: 53 IQYFELITLQ*FQVKKCLSFYITNMSIL----AYNGGAVVAMK 169
++YF+ + + +VK SF+I N +L AYNG V +K
Sbjct: 441 LKYFDQVVVGLKEVKDAFSFFIFNRFVLVYVPAYNGVDPVTLK 483
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 26.2 bits (55), Expect = 4.1
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 175 RLCGHRYGQAVWYTSSNSINQLPKSIPNGTHIV 273
RL G Y V Y+S NS+ LPK P+ H+V
Sbjct: 239 RLRGKTYDYKVEYSSINSLFLLPK--PDEQHVV 269
>SPAC24B11.13 |hem3|SPAC806.01|hydroxymethylbilane
synthase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +1
Query: 223 NSINQLPKSIPNGTHIVCRPSRS 291
+S+ LP +P+G I C P RS
Sbjct: 84 HSLKDLPSEMPDGMVIACIPKRS 106
>SPBC4B4.01c |||fumble family pantothenate kinase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 403
Score = 25.0 bits (52), Expect = 9.5
Identities = 8/19 (42%), Positives = 17/19 (89%)
Frame = +2
Query: 200 KRFGIQAQTVSTNFPKVFQ 256
+RFG+++ T++++F KVF+
Sbjct: 281 ERFGLKSTTIASSFGKVFR 299
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 25.0 bits (52), Expect = 9.5
Identities = 20/75 (26%), Positives = 34/75 (45%), Gaps = 6/75 (8%)
Frame = -3
Query: 336 ILNFSL*NTVWVSVARPGRPTYNVGPIWNTFGKLVDTV*ACIPNR------LSVAMATQS 175
++NFS T + S P+ N P W+ + + +PN +V A
Sbjct: 449 VVNFSTPITNFFSKIYKDNPSLNP-PSWHLITLVHRNEKSSLPNLELYIDGSAVERAACP 507
Query: 174 WPFMATTAPPLYASI 130
+PF+++T PLY S+
Sbjct: 508 YPFLSSTLQPLYVSL 522
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,851,693
Number of Sequences: 5004
Number of extensions: 61345
Number of successful extensions: 168
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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