Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P06_F_P04
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 101 7e-23
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 64 1e-11
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 62 9e-11
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 47 2e-06
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 45 1e-05
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 43 4e-05
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 37 0.003
SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Sch... 29 0.44
SPBC3F6.03 |trr1|caf4|thioredoxin reductase Trr1|Schizosaccharom... 26 4.1
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 26 5.5
SPCC23B6.01c |||oxysterol binding protein |Schizosaccharomyces p... 25 9.5
SPBC19C7.07c |sen34||tRNA-splicing endonuclease subunit Sen34|Sc... 25 9.5
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi... 25 9.5
>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 401
Score = 101 bits (243), Expect = 7e-23
Identities = 45/84 (53%), Positives = 61/84 (72%)
Frame = +2
Query: 350 RYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTN 529
RY RQ+LLS+IG+ GQ+ + + VL++GAGGLGCPA YL AGIG +GI+D D VD +N
Sbjct: 23 RYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSN 82
Query: 530 VHRQLLHHESNENTSKAFSAAESL 601
+HRQ++H S + KA SA + L
Sbjct: 83 LHRQIIHSTSKQGMHKAISAKQFL 106
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 64.5 bits (150), Expect = 1e-11
Identities = 29/64 (45%), Positives = 44/64 (68%)
Frame = +2
Query: 410 SAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSA 589
S+K+LI+GAGGLGC LA +G ++ ++D D +D+TN++RQ L +ESN + KA A
Sbjct: 44 SSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNIDEPKANVA 103
Query: 590 AESL 601
A +
Sbjct: 104 ASMI 107
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 61.7 bits (143), Expect = 9e-11
Identities = 30/71 (42%), Positives = 44/71 (61%)
Frame = +2
Query: 386 VEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNE 565
VE SAKVL+VGAGG+GC L +G+ E+ I+D D +DL+N++RQ L + +
Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV 76
Query: 566 NTSKAFSAAES 598
KA AA++
Sbjct: 77 KQPKAIVAAKT 87
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 47.2 bits (107), Expect = 2e-06
Identities = 32/101 (31%), Positives = 50/101 (49%), Gaps = 5/101 (4%)
Frame = +2
Query: 314 SVDACLPKWAIERYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCP-----AAMYLAGA 478
S + C P+ RY QI + G E Q KI S +VGAG +GC A M +A
Sbjct: 399 SEETCKPRGC--RYDGQIAV--FGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATG 454
Query: 479 GIGEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSAAESL 601
G I + D D+++ +N++RQ L + K+ A+ ++
Sbjct: 455 ESGHISVTDMDSIEKSNLNRQFLFRPRDVGKLKSECASTAV 495
Score = 32.7 bits (71), Expect = 0.048
Identities = 20/76 (26%), Positives = 36/76 (47%)
Frame = +2
Query: 353 YSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNV 532
YSRQ+ + +G E ++ + VLI+G GLG A + AG+ + + D + ++
Sbjct: 20 YSRQLYV--LGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDL 77
Query: 533 HRQLLHHESNENTSKA 580
Q E + +A
Sbjct: 78 SSQYFLTEDDIGVPRA 93
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 44.8 bits (101), Expect = 1e-05
Identities = 21/74 (28%), Positives = 44/74 (59%)
Frame = +2
Query: 383 GVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESN 562
G +G ++ ++ V++VG GG+G LA +G+ +I IVD+D V L++++R + +
Sbjct: 117 GEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSLNRHSIATLQD 176
Query: 563 ENTSKAFSAAESLR 604
T K + ++++
Sbjct: 177 VGTPKTLAIKKAIK 190
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 42.7 bits (96), Expect = 4e-05
Identities = 28/83 (33%), Positives = 39/83 (46%), Gaps = 2/83 (2%)
Frame = +2
Query: 401 KICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLL--HHESNENTS 574
+I ++K L++GAG LGC A L G+ + VDY V +N RQ L +
Sbjct: 332 RIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVDYSTVSYSNPVRQSLFTFEDCKRKLP 391
Query: 575 KAFSAAESLRWCVT*TLRRGRNI 643
KA AA+ L+ G NI
Sbjct: 392 KAECAAQRLKEIYPNMFSTGYNI 414
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 36.7 bits (81), Expect = 0.003
Identities = 26/87 (29%), Positives = 39/87 (44%)
Frame = +2
Query: 344 IERYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDL 523
+++Y RQ+ L EGQ I + V ++ A +GC A L GIG +VD +VD
Sbjct: 7 MQKYDRQVRLWK--AEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDF 64
Query: 524 TNVHRQLLHHESNENTSKAFSAAESLR 604
+ E S+A A L+
Sbjct: 65 SMDGMNFFIQYDQEGKSRARCTASLLQ 91
>SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 424
Score = 29.5 bits (63), Expect = 0.44
Identities = 32/116 (27%), Positives = 50/116 (43%), Gaps = 8/116 (6%)
Frame = +2
Query: 284 SQPISDASKSSVDACLPKWAIERYSRQILLSDIGV--EGQVKICSAKVLIVGAGGLGCP- 454
SQ I V C+P +IE Y + + +D+GV + +V G G P
Sbjct: 293 SQYIKKHPLHKVRFCMPWLSIEDYPQVMACADLGVCLHTSSSGLDLPMKVVDLFGCGVPV 352
Query: 455 -AAMYLAGAGI---GEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSAAES-LRW 607
A Y + + GE G++ D+ L+ + LL H + N+ K + ES RW
Sbjct: 353 IALSYPTISELVHDGENGLIVNDSKALSKKMQYLLTHANELNSLKLGALKESEYRW 408
>SPBC3F6.03 |trr1|caf4|thioredoxin reductase
Trr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 322
Score = 26.2 bits (55), Expect = 4.1
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +2
Query: 416 KVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAV 517
KV+I+G+G G AA+YLA GE+ V Y+ +
Sbjct: 5 KVVIIGSGPAGHTAAIYLAR---GELKPVMYEGM 35
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 25.8 bits (54), Expect = 5.5
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +2
Query: 248 SDQQSSKELNNHSQPISDASKSSVDACLPKWAIERYSRQIL 370
S++++ K + N +PIS S S+V + ++ R RQIL
Sbjct: 567 SNKEAVKPIKNKPKPISFESLSAVGNLIISDSLSRIVRQIL 607
>SPCC23B6.01c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 489
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -3
Query: 502 HDAYFANARPGKVHGSRTSQ 443
HD Y +ARP + G R SQ
Sbjct: 296 HDVYLVSARPLEEQGERESQ 315
>SPBC19C7.07c |sen34||tRNA-splicing endonuclease subunit
Sen34|Schizosaccharomyces pombe|chr 2|||Manual
Length = 284
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = +2
Query: 515 VDLTNVHRQLLHHESNENTSKAFSAAESLRW 607
VD T VH+QLL + + ++ + +SL +
Sbjct: 73 VDDTKVHKQLLENTTKDDVKQCLKKRQSLAY 103
>SPAC20G8.04c |||mitochondrial electron transfer
flavoprotein-ubiquinone
oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 632
Score = 25.0 bits (52), Expect = 9.5
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = -2
Query: 239 FLIPEVHFPFPL 204
FLIP++HFP P+
Sbjct: 171 FLIPKLHFPIPV 182
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,573,445
Number of Sequences: 5004
Number of extensions: 52169
Number of successful extensions: 138
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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