Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P06_F_J24
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 171 1e-43
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 99 5e-22
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 96 3e-21
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 90 3e-19
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 83 4e-17
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 81 1e-16
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 69 6e-13
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 29 0.58
SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosacch... 25 7.2
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 9.5
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 171 bits (415), Expect = 1e-43
Identities = 79/150 (52%), Positives = 109/150 (72%)
Frame = +2
Query: 179 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRAR 358
G+TSV + G + A V +Q+KVPD+L+D +V H+F +T IGCVMTG IAD+R+QV RAR
Sbjct: 34 GITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVSRAR 93
Query: 359 YEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTD 538
EAA ++YK G +P +L +R+A+I+QV TQ A MRPLG +M L+A DDE GP ++K D
Sbjct: 94 SEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMTLVAVDDEIGPSLFKLD 153
Query: 539 PSGYYCSYKAVAAGAKTVDGNAYLEKKLKK 628
P+G+Y YKA +AG K + +LEK+ KK
Sbjct: 154 PAGFYIGYKATSAGPKQTETINWLEKRFKK 183
Score = 62.5 bits (145), Expect = 5e-11
Identities = 28/50 (56%), Positives = 33/50 (66%)
Frame = +1
Query: 94 GFDRHITIFSPEGRLYQVEYALKAINQGWVDICGITRYRCSCSGSSTQGP 243
GFDR IT+FSPEGRLYQVEYA KA N + G+T C+C S + P
Sbjct: 6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVP 55
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 99.1 bits (236), Expect = 5e-22
Identities = 60/160 (37%), Positives = 87/160 (54%), Gaps = 2/160 (1%)
Frame = +2
Query: 179 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRAR 358
G+TSV ++ TD V+A ++K L S+ + A+T IG V +GM D R V ++R
Sbjct: 31 GVTSVGIKATDGVVLATEKKPTSELAIGASLEKVCAITPDIGMVYSGMGPDFRVLVDKSR 90
Query: 359 YEAANWQYK--YGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYK 532
+ A YK Y P IL + IA + Q TQ+ +RP G S+ L+A DE GP +Y+
Sbjct: 91 -KVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGGVRPFGVSL-LVAGMDEKGPSLYQ 148
Query: 533 TDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDD 652
DPSG Y ++KA A G + +LEK+ +L DD
Sbjct: 149 VDPSGTYFAWKATAIGKSSTAAKTFLEKRY--NDELELDD 186
Score = 36.3 bits (80), Expect = 0.004
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +1
Query: 109 ITIFSPEGRLYQVEYALKAINQG 177
+T FSP G+L Q+EYAL A+N G
Sbjct: 9 LTTFSPNGKLVQIEYALNAVNAG 31
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 96.3 bits (229), Expect = 3e-21
Identities = 58/163 (35%), Positives = 88/163 (53%), Gaps = 3/163 (1%)
Frame = +2
Query: 173 RXGLTSVALRGTDAAVVAAQRK-VPDRLLDPVSVTHLFALTDRIGCV-MTGMIADSRSQV 346
R G T++ALRG + V+ +RK VP L VS A+ D C+ G+ AD+R +
Sbjct: 28 RRGTTAIALRGNECIVIGVERKNVPK--LQNVSNFQKIAMVDNHVCLAFAGLNADARILI 85
Query: 347 QRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYD-DEAGPC 523
+AR EA N + + + L R +A + Q YTQ+ +RP G S ++ +D + P
Sbjct: 86 DKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFDVGDNTPR 145
Query: 524 VYKTDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDD 652
VY+T+P+G Y ++KA A G + YLEK K+ LS D+
Sbjct: 146 VYQTEPAGIYNAWKATAIGRASKAAREYLEKNWKE--GLSRDE 186
Score = 41.1 bits (92), Expect = 1e-04
Identities = 15/29 (51%), Positives = 24/29 (82%)
Frame = +1
Query: 91 AGFDRHITIFSPEGRLYQVEYALKAINQG 177
+G+DR +++FSP+GRL QVEY +A+ +G
Sbjct: 2 SGYDRALSVFSPDGRLLQVEYGQEAVRRG 30
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 89.8 bits (213), Expect = 3e-19
Identities = 47/144 (32%), Positives = 74/144 (51%)
Frame = +2
Query: 185 TSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRARYE 364
T + ++ D ++A ++ V +LL P + ++ IG TG I D + V+RAR E
Sbjct: 35 TCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRARDE 94
Query: 365 AANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTDPS 544
A +W+ YGS IP ++ R+ + Q++T + +RP G + YD E GP +Y +P+
Sbjct: 95 ATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSE-GPHLYMVEPN 153
Query: 545 GYYCSYKAVAAGAKTVDGNAYLEK 616
G Y Y AAG LEK
Sbjct: 154 GVYWGYNGAAAGKGRQVARNELEK 177
Score = 32.7 bits (71), Expect = 0.047
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +1
Query: 94 GFDRHITIFSPEGRLYQVEYALKAI 168
G+D + FSP+GRL+Q EYA KA+
Sbjct: 7 GYDLGL-FFSPDGRLFQAEYAYKAV 30
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 82.6 bits (195), Expect = 4e-17
Identities = 45/158 (28%), Positives = 85/158 (53%), Gaps = 6/158 (3%)
Frame = +2
Query: 173 RXGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQR 352
+ G T++ ++ DA V+ ++++ L++ SV LF + IGC ++G+ AD+R+ ++
Sbjct: 32 KLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEH 91
Query: 353 ARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAE------MRPLGCSMMLIAYDDEA 514
AR + N ++ Y + + I D++ + + + RP G + +LIA DE
Sbjct: 92 ARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVA-LLIAGIDEH 150
Query: 515 GPCVYKTDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKK 628
GP +Y ++PSG Y Y+A A G+ + + L K+ K
Sbjct: 151 GPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHK 188
Score = 41.9 bits (94), Expect = 8e-05
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +1
Query: 97 FDRHITIFSPEGRLYQVEYALKAINQG 177
+DR + FSPEGRL+QVEYA++AI G
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKLG 34
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 81.0 bits (191), Expect = 1e-16
Identities = 43/149 (28%), Positives = 79/149 (53%), Gaps = 1/149 (0%)
Frame = +2
Query: 209 DAAVVAAQRKVPDRLLDPV-SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYK 385
D V+AA++KV +LL+ S L+ + D + C + G+ AD+ + AR +
Sbjct: 41 DGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQT 100
Query: 386 YGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYK 565
+ E+P L RR+ D+ Q YTQ +RP G S + +D G +++++PSG Y S++
Sbjct: 101 FNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQ 160
Query: 566 AVAAGAKTVDGNAYLEKKLKKRSDLSTDD 652
A + G + + + ++ K D++ D+
Sbjct: 161 ANSIGGNSTSVQSLMRQEYK--DDINLDE 187
Score = 48.0 bits (109), Expect = 1e-06
Identities = 22/32 (68%), Positives = 24/32 (75%)
Frame = +1
Query: 88 SAGFDRHITIFSPEGRLYQVEYALKAINQGWV 183
S +D TIFSPEGRLYQVEYAL+AIN V
Sbjct: 2 SRSYDSRTTIFSPEGRLYQVEYALEAINHAGV 33
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 68.9 bits (161), Expect = 6e-13
Identities = 43/160 (26%), Positives = 75/160 (46%)
Frame = +2
Query: 173 RXGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQR 352
+ G +V L AV+ A ++ + L L + D IG + G+ D+R
Sbjct: 30 KQGSATVGLVSKTHAVLVALKRNAEELSSYQK--KLIRIDDHIGIAIAGLAPDARVLSNY 87
Query: 353 ARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYK 532
+ EA + + + IPV L ++A+ +Q+ TQ RP G ++I Y DE+GP + +
Sbjct: 88 MKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGY-DESGPHLLE 146
Query: 533 TDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDD 652
PSG Y + G+++ Y+E+ L D S ++
Sbjct: 147 FQPSGLVLEYLGTSMGSRSQSARTYIERNLDTFPDSSREE 186
Score = 39.9 bits (89), Expect = 3e-04
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +1
Query: 97 FDRHITIFSPEGRLYQVEYALKAINQG 177
+D T +SP+GRL+QVEYAL+AI QG
Sbjct: 6 YDGDATTWSPQGRLHQVEYALEAIKQG 32
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 29.1 bits (62), Expect = 0.58
Identities = 15/62 (24%), Positives = 25/62 (40%)
Frame = +2
Query: 317 GMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLI 496
GM +R+ Q +E +W Y + V LCR + ++ N + + LI
Sbjct: 606 GMCPYTRAVSQHVEWENDSWMYVLNVSLQVAKLCRHVGNVFMELNTNKLANAINYLISLI 665
Query: 497 AY 502
Y
Sbjct: 666 LY 667
>SPAC343.17c |||WD repeat protein, human WDR70
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 576
Score = 25.4 bits (53), Expect = 7.2
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 371 WQPRSELVVLETGYQQSSR 315
WQP S + L TGY ++R
Sbjct: 205 WQPDSSQIFLTTGYDSTAR 223
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.0 bits (52), Expect = 9.5
Identities = 20/90 (22%), Positives = 38/90 (42%), Gaps = 1/90 (1%)
Frame = +2
Query: 266 SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADIS-Q 442
++ +L+ +I CV AD Q QR + + + G P HI I + S +
Sbjct: 509 NLLNLYPDLQKIPCVFAATKADLDRQQQRYPVQPDEFTKQLGLPSPTHISTAAIWNTSKE 568
Query: 443 VYTQNAEMRPLGCSMMLIAYDDEAGPCVYK 532
+ Q AE S ++ ++++ Y+
Sbjct: 569 FFIQLAESAQYPASSIIRIPEEDSNKTNYQ 598
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,417,857
Number of Sequences: 5004
Number of extensions: 45481
Number of successful extensions: 134
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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