Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P06_F_F24
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 37 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 33 0.036
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 32 0.083
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 30 0.25
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 29 0.77
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 2.4
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 27 3.1
SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit L37|... 27 3.1
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 4.1
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 5.5
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 25 7.2
SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosacc... 25 7.2
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 25 7.2
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 25 7.2
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 25 9.5
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 9.5
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 345 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 503
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
Score = 28.7 bits (61), Expect = 0.77
Identities = 14/60 (23%), Positives = 29/60 (48%)
Frame = +3
Query: 357 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSES 536
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 33.1 bits (72), Expect = 0.036
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 339 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 506
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 31.1 bits (67), Expect = 0.15
Identities = 17/76 (22%), Positives = 32/76 (42%), Gaps = 6/76 (7%)
Frame = +3
Query: 333 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL-- 506
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 507 ----IKIRPPMSESRR 542
I R P+ E +R
Sbjct: 99 MTEKIVERDPLEEIKR 114
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 31.9 bits (69), Expect = 0.083
Identities = 25/113 (22%), Positives = 51/113 (45%), Gaps = 1/113 (0%)
Frame = +3
Query: 228 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 407
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 408 KETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKIRPPMSESRRNIV*QRI 563
+ NK E + F +D D G IS E + ++ + + R Q+I
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQI 135
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 30.3 bits (65), Expect = 0.25
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +3
Query: 330 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSG 482
G+ ++FR + S +LN+ EF + GL + E LF + G
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG 538
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 28.7 bits (61), Expect = 0.77
Identities = 24/76 (31%), Positives = 35/76 (46%)
Frame = +1
Query: 400 MASRKQGWNLIKVKPKNSSVNSTQTVVAQSVLMNSLLKSVLPCRNRVVTLXNNASFKKLD 579
M S K+ K P +S+ S+Q V AQ+ S +++ L +F LD
Sbjct: 1 MFSSKENSLGAKRAPFSSNTTSSQRVAAQAAKRASSGAFAQLTSSQIQELKE--AFALLD 58
Query: 580 KTGDGAITIDDIKGCL 627
K GDG I +D+K L
Sbjct: 59 KDGDGNIGREDVKTML 74
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 2.4
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 165 KPKTSSNIAWHRPMSAGSVQEEELMQK 245
KPK S ++W+ + GS +E EL+ K
Sbjct: 56 KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 26.6 bits (56), Expect = 3.1
Identities = 12/41 (29%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = +2
Query: 125 GCNVILNSKNVFRKTE--NIFQHCVAQTDVCWFRPGGRADA 241
GC + N+ E +F+ A + CW PG A+A
Sbjct: 331 GCRYVAEGSNMGSSAEAVEVFEKSRASGEGCWLAPGKAANA 371
>SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit
L37|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 26.6 bits (56), Expect = 3.1
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +1
Query: 382 TKKNSFMASRKQGWNLIKVKPKNSSVNSTQTVVAQSVLMNSLLKSVLP 525
+ +NS + K+ + +V PK NS T AQ V + SVLP
Sbjct: 32 SSRNSSSSLVKRSYVSSRVSPKKPQHNSDATSSAQKVANKTHTSSVLP 79
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = +3
Query: 420 LELNKSEA--EELFSQFDTDSSGSISLDEF 503
L LN S EE F + D D SG +S +EF
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEF 355
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -1
Query: 402 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLGT 304
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 25.4 bits (53), Expect = 7.2
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +3
Query: 378 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFL 506
LNK EF K+ + S AE +F+ FD D +G I EF+
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFI 86
>SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog
Taz1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 663
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +3
Query: 444 EELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIV 551
E + F TDS+ SI EF++ P S N+V
Sbjct: 273 ELVIRYFGTDSNPSIDASEFILSCLPYTSLDALNVV 308
>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 151 KCI*ENRKHLPTLRGTDRCLLVPSRRK 231
+C+ E+R+H P L +D LL +RK
Sbjct: 82 RCMEEDREHNPFLSSSDNQLLSRKKRK 108
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 25.4 bits (53), Expect = 7.2
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +3
Query: 216 SVQEEELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEF 395
S Q E + R M L +L + ++ ILGL R D+G K+ NK+
Sbjct: 182 SYQNLESINSWLRLMASLYKDLSELVPVGITAEGRTILGLKLNGRHPSDNGEKIRNKKVI 241
Query: 396 L-----YGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 503
+ + + G+ A +L +++D+D LD+F
Sbjct: 242 IIQGGSHAREWIGIPSVCYAAWQLLAKYDSDGHVRKLLDKF 282
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 342 IFRRMDDDGSKLLNKEEFLYGIKETGL-ELNKSEAE 446
IFRR+ D S + ++ FL+ G+ E+ +EAE
Sbjct: 377 IFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAE 412
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/39 (28%), Positives = 20/39 (51%)
Frame = +3
Query: 390 EFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 506
E+ G+ + + S LF +FD +GS+SL + +
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLV 602
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,382,492
Number of Sequences: 5004
Number of extensions: 46216
Number of successful extensions: 157
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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