Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P06_F_E20
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyce... 73 5e-14
SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomy... 30 0.33
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 29 0.44
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 29 0.76
SPBC409.08 |||spermine family transporter |Schizosaccharomyces p... 27 2.3
SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr 2|||... 27 3.1
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 26 5.4
SPAPB1A11.02 |||esterase/lipase |Schizosaccharomyces pombe|chr 1... 26 5.4
SPAC1071.11 |||NADH-dependent flavin oxidoreductase |Schizosacch... 25 9.4
SPAC1F8.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 9.4
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 9.4
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc... 25 9.4
>SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 520
Score = 72.5 bits (170), Expect = 5e-14
Identities = 53/192 (27%), Positives = 89/192 (46%), Gaps = 21/192 (10%)
Frame = +2
Query: 131 GLIQGSWKVSTKGRPYAS-FQGVPYARPPIGKYRFREPQQLK---PWTGVWDATRTLSSC 298
G++ G + G+ F G+ YA+PP+GK R+R P L+ ++G ++ +T+
Sbjct: 2 GVLHGLTVLDENGKEKCHRFTGIRYAKPPVGKLRWRRPVTLEDGYDYSGDYNQFKTICPQ 61
Query: 299 LQYDPFVGTITG-----SENCLFVNVYTPK-LTPTASLPVMVFIHGGAFMYGEGGFY--- 451
Y+ + E+CLF+N++ P P PV+ FIHGG G Y
Sbjct: 62 PFYNNRKNQVRNPDFKYDEDCLFLNIWVPAGEKPAEGWPVLYFIHGGWLQVGNPLHYRQC 121
Query: 452 DAGNLM----DRDMVVVTLNYRLGPLGFLS----TGDEVAPGNNGLKDQSFALHWVKNNI 607
D +L ++V+ +RL GFL+ ++ N G DQ L W +I
Sbjct: 122 DPQDLQADGSPAKFILVSPGHRLNLFGFLAGKELLEEDPKSSNFGFWDQRLGLEWTYKHI 181
Query: 608 QMFGGNPDSVTL 643
+ FGGN +++ +
Sbjct: 182 ESFGGNKENIAV 193
>SPAC3H1.09c |||vacuolar amino acid transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 656
Score = 29.9 bits (64), Expect = 0.33
Identities = 16/61 (26%), Positives = 28/61 (45%)
Frame = -3
Query: 499 IQRDDHHIPVHQISGIVKSPFTVHEGPAMDENHNRKRGRWSQLGCVHIHEQTILTTSYSS 320
I + ++P + +P V+ G DENHN ++G+ L H + + +TS
Sbjct: 50 ISPSESNLPNNVAENTTDTP--VNYGSIRDENHNSRKGKDVTLNSDEAHSENVPSTSEDP 107
Query: 319 D 317
D
Sbjct: 108 D 108
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 29.5 bits (63), Expect = 0.44
Identities = 23/88 (26%), Positives = 44/88 (50%), Gaps = 8/88 (9%)
Frame = -3
Query: 625 VPAKHLYVVLDPVEGEGLVFQAVVAWRYLVACAK--ET*RSQSIIQRDDHHIPVHQ---- 464
+P+ + L P+E L + V + L A ++ RS++++Q D+HH +
Sbjct: 310 IPSPYQPSSLQPLESRKLHSKVDVHSKKLNALSQLNPILRSENVLQNDNHHSSLSMDNDP 369
Query: 463 --ISGIVKSPFTVHEGPAMDENHNRKRG 386
+S ++ TV E P +D+N +K+G
Sbjct: 370 TNVSTKNRNNQTVGEHPYVDDNKKKKKG 397
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 28.7 bits (61), Expect = 0.76
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +2
Query: 248 LKPWTGVWDATRTLSSCLQYDPFVGTITGSE 340
LK W+ +W+ R L ++Y +V T+ GSE
Sbjct: 108 LKIWSKLWEDNRRLKKIIKYTSYV-TVKGSE 137
>SPBC409.08 |||spermine family transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 539
Score = 27.1 bits (57), Expect = 2.3
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +2
Query: 101 GFNPVVRVSQGLIQGSWKVSTKGRPYASFQGVPYARPPIG 220
G P+ ++ G+I W+ +G A F PYA P +G
Sbjct: 204 GCTPLT-LAGGVISDVWETRERGLAIAFFAAGPYAGPTLG 242
>SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 462
Score = 26.6 bits (56), Expect = 3.1
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -1
Query: 432 YMKAPPWMKTITGREAVGVSLGVYTFTNRQFSLPVIVPTNGS 307
YM+A P ++ + E V G+Y + R+ LPVI N S
Sbjct: 145 YMRATPMVRALESLEHV--FSGLYPESKRKMGLPVIFTRNWS 184
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 5.4
Identities = 17/61 (27%), Positives = 26/61 (42%)
Frame = -3
Query: 358 IHEQTILTTSYSSDEWIVL*TRGKSPRGIPNSSPRLQLLGFPKSILSNWWPCVGNSLKAS 179
IHE + S +S+ ++ + K IPN P LL I ++ C G AS
Sbjct: 311 IHEGLLFIGSKNSESKLINLSTLKDVDSIPNLGPIHDLLVLKNDIEKSFLVCAGTPRNAS 370
Query: 178 V 176
+
Sbjct: 371 L 371
>SPAPB1A11.02 |||esterase/lipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 5.4
Identities = 25/93 (26%), Positives = 38/93 (40%), Gaps = 3/93 (3%)
Frame = +2
Query: 359 VYTPKLTPTASLPVMVFIHGGAF-MYGEGGFYDAGNLMDRDM--VVVTLNYRLGPLGFLS 529
V+TP P +MVF H + M G ++ V V+++YRL P
Sbjct: 79 VFTPVSVPADYRSLMVFYHSSGWCMRGVRDDDSLFKILTPKFGCVCVSVDYRLAP----- 133
Query: 530 TGDEVAPGNNGLKDQSFALHWVKNNIQMFGGNP 628
+ +N D + WV +NI+ G NP
Sbjct: 134 -ESKFPVAHNDAID---SFKWVASNIEKLGANP 162
>SPAC1071.11 |||NADH-dependent flavin oxidoreductase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 9.4
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = -1
Query: 375 SLGVYTFTNRQFSLPVIVPTNGSYCRHEERVLVASQTPV 259
++ + R+F+ PV++ T+G H + +S TPV
Sbjct: 38 NVNAFRLLMRRFAQPVVIITSGFADGHRAGMTASSFTPV 76
>SPAC1F8.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 120
Score = 25.0 bits (52), Expect = 9.4
Identities = 7/16 (43%), Positives = 12/16 (75%)
Frame = -1
Query: 609 CMLFLTQWRAKDWSFK 562
C F++ W+ KD+SF+
Sbjct: 20 CCAFMSHWKLKDFSFR 35
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 25.0 bits (52), Expect = 9.4
Identities = 15/47 (31%), Positives = 19/47 (40%)
Frame = -1
Query: 561 PLLPGATSSPVLRKPRGPNL*FNVTTTISLSIRFPAS*NPPSPYMKA 421
P LP TS P N+ N T+ + FP PP P + A
Sbjct: 911 PPLPVKTSLNTFSHPDSVNIVANDTSVAGVMPAFPPPPPPPPPLVSA 957
>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 765
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/48 (27%), Positives = 25/48 (52%)
Frame = +1
Query: 10 IQITGADARANEDAIKIIFPRCRGGYGDRSRFQPCSEGVPRAYTRQLE 153
++ TGA + D + I FP+C G + PC+ G+ + + +L+
Sbjct: 261 LRCTGAAVKIASDLLGIAFPKC-GSRIELFCGGPCTVGLGQVVSTELK 307
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,029,874
Number of Sequences: 5004
Number of extensions: 69133
Number of successful extensions: 190
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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