Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P06_F_A23
(613 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 35 0.008
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 31 0.13
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 30 0.23
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.23
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.30
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.53
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 0.70
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 0.93
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 1.2
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 1.2
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 1.6
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 27 2.1
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 2.8
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 2.8
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 2.8
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 26 3.7
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 3.7
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 4.9
SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc... 26 4.9
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 4.9
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 6.5
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 25 6.5
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 8.6
SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit... 25 8.6
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 8.6
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 25 8.6
SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 8.6
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 8.6
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 35.1 bits (77), Expect = 0.008
Identities = 28/107 (26%), Positives = 55/107 (51%), Gaps = 6/107 (5%)
Frame = +1
Query: 190 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 360
T+E ++SL KSK + F + ++ + +L N + + L + +A KA+++LEQ
Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65
Query: 361 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 492
+ + E L K H + E+ + +EKL A ++ + S++L
Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 31.1 bits (67), Expect = 0.13
Identities = 34/148 (22%), Positives = 60/148 (40%), Gaps = 4/148 (2%)
Frame = +1
Query: 4 THSSVLQSWFASP----PHSVSRQHIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHH 171
T S S+++ P P S S + A + L + V R F + +EH+
Sbjct: 154 TSSVASYSYYSGPNPATPSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN 213
Query: 172 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEA 351
K+LE+Q + L +SKDA A S+ + S + +L N K++ +
Sbjct: 214 -HPLSKSLEEQSSFLEQSKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRAS 271
Query: 352 LEQSRQNIERTAEELRKAHPDVEKNATA 435
L+ ++ R P+V + +A
Sbjct: 272 LQHKIMSLSRNIIRRVFHKPEVHLDPSA 299
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 30.3 bits (65), Expect = 0.23
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 8/80 (10%)
Frame = +1
Query: 337 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 492
K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186
Query: 493 AKKVSSNVQETNEKLAPKIK 552
K + T +KL KI+
Sbjct: 187 YKDMEQENASTIQKLTSKIR 206
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 30.3 bits (65), Expect = 0.23
Identities = 17/81 (20%), Positives = 38/81 (46%)
Frame = +1
Query: 289 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 468
N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + +
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396
Query: 469 TVQESQKLAKKVSSNVQETNE 531
+QE ++ K + Q+ +E
Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.30
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +1
Query: 256 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 393
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 29.1 bits (62), Expect = 0.53
Identities = 17/69 (24%), Positives = 38/69 (55%)
Frame = +1
Query: 337 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 516
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 517 QETNEKLAP 543
+ ++ ++P
Sbjct: 186 RSSSRSISP 194
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 0.70
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +1
Query: 235 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 390
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.3 bits (60), Expect = 0.93
Identities = 24/88 (27%), Positives = 45/88 (51%)
Frame = +1
Query: 160 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 339
IE K F K ++ NS K+ +A F +G++ ++ + A ++L L +
Sbjct: 73 IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125
Query: 340 AKEALEQSRQNIERTAEELRKAHPDVEK 423
+EQ+R E T E++++A P++EK
Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 1.2
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +1
Query: 130 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 300
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 301 KSLQGALGDA 330
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 1.2
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +1
Query: 190 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 351
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 352 LEQSRQNIERTAEE 393
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 1.6
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 367 ASTVPKPPWPCRSRLRALPG 308
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 27.1 bits (57), Expect = 2.1
Identities = 17/73 (23%), Positives = 38/73 (52%)
Frame = +1
Query: 352 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 531
++ Q+IE T L K D+E++ +++ + V + Q+ ++++ +Q+T E
Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553
Query: 532 KLAPKIKAAYDDF 570
L+ K + DD+
Sbjct: 554 VLSKSSKES-DDY 565
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 136 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 255
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.6 bits (56), Expect = 2.8
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +1
Query: 304 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 459
S++ L + N + KE +E + RT +E EKN LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 26.6 bits (56), Expect = 2.8
Identities = 22/74 (29%), Positives = 32/74 (43%)
Frame = +1
Query: 265 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 444
S + + A K L GA KAKE ++ R +RTA E+RK +E+ R
Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199
Query: 445 KLQAAVQNTVQESQ 486
+ A Q + Q
Sbjct: 200 ERAAEAQRVAGKEQ 213
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 26.2 bits (55), Expect = 3.7
Identities = 18/71 (25%), Positives = 35/71 (49%)
Frame = +1
Query: 367 QNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPK 546
+NI + E+ R + NA+ + K Q + T++ Q+LAK + +++ E+L+
Sbjct: 136 KNILKAIEDSRYPFVANKLNASTIEVKPQ---RTTLESRQQLAKVLEGYAKDSREQLSAM 192
Query: 547 IKAAYDDFAKN 579
+ AKN
Sbjct: 193 RTELKKEIAKN 203
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 3.7
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +1
Query: 172 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 330
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
Frame = +1
Query: 499 KVSSNVQET--NEKLAPKIKAAYD 564
+V N++ET EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322
>SPAC23H4.11c |cnl2||centromere localized protein
Cnl2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 25.8 bits (54), Expect = 4.9
Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +1
Query: 343 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 516
++ L + R NI ++ + K+ D + N L+ A+ + V+E ++
Sbjct: 53 QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112
Query: 517 QETNEKLAPKIKAAY 561
E NE+L+ +++AY
Sbjct: 113 SEINERLSEAVQSAY 127
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 4.9
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 100 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 276
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 277 LQQL 288
Q+
Sbjct: 73 NNQI 76
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 25.4 bits (53), Expect = 6.5
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +1
Query: 394 LRKAHPDVEKNATALREKLQAAVQNTVQE 480
L AH D+E T++RE+L++ + +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295
>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1108
Score = 25.4 bits (53), Expect = 6.5
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +1
Query: 331 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 426
N K KE E++ + EELR+A PD E++
Sbjct: 22 NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.0 bits (52), Expect = 8.6
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = +1
Query: 466 NTVQESQKL-AKKVSSNVQETNEKLAPKIKAAYDDFAKNTQXVIRNIQE 609
NT ++S L KKV + +E + KI+ D+FA + V+RNI+E
Sbjct: 4651 NTQKKSSILDIKKVYYDTKEDGT-MDLKIQPYIDEFAFDYYLVVRNIEE 4698
>SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit
subunit Ker1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 25.0 bits (52), Expect = 8.6
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1
Query: 256 KDGSESVLQQLNAFAKSLQG 315
K GSESVL QLN L+G
Sbjct: 40 KSGSESVLSQLNRVLMYLKG 59
>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
transporter Bfr1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1530
Score = 25.0 bits (52), Expect = 8.6
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -3
Query: 446 FSRRAVAFFSTSGWALRSSSAVRSMF 369
FSR V FFS AL+S S + +MF
Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 25.0 bits (52), Expect = 8.6
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 322 GDANGKAKEALEQSRQNIERTAEE 393
G NG+AKE E+ R ++T E+
Sbjct: 473 GKGNGRAKETPEEKRARKKKTKED 496
>SPBC27B12.12c |||CorA family magnesium ion transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.0 bits (52), Expect = 8.6
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 373 CSASTVPKPPWPCRSRLRALPGDS 302
C +T+PKPP+ + L LP S
Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKS 313
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.0 bits (52), Expect = 8.6
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +1
Query: 358 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 510
++RQ E A+ +RK+ PDV + ++ +V + +E + +SS
Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.312 0.123 0.335
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,925,749
Number of Sequences: 5004
Number of extensions: 32377
Number of successful extensions: 149
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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