Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_P15
(667 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P91641 Cluster: Mediator complex subunit 20; n=5; Endop... 121 2e-26
UniRef50_Q9H944 Cluster: Mediator of RNA polymerase II transcrip... 100 3e-20
UniRef50_A7RT91 Cluster: Predicted protein; n=1; Nematostella ve... 90 4e-17
UniRef50_Q9N5A1 Cluster: Mediator protein 20; n=2; Caenorhabditi... 56 1e-06
UniRef50_Q6NPF4 Cluster: At2g28230; n=9; Magnoliophyta|Rep: At2g... 49 1e-04
UniRef50_Q54FL3 Cluster: Putative uncharacterized protein; n=1; ... 49 1e-04
UniRef50_Q5DCZ1 Cluster: SJCHGC05429 protein; n=1; Schistosoma j... 47 5e-04
UniRef50_Q55B78 Cluster: Putative uncharacterized protein; n=1; ... 33 4.7
UniRef50_Q16PK6 Cluster: Serine protease, putative; n=7; Aedes a... 33 4.7
UniRef50_A2DDB2 Cluster: Putative uncharacterized protein; n=1; ... 33 4.7
UniRef50_Q1E4V1 Cluster: Putative uncharacterized protein; n=1; ... 33 4.7
UniRef50_Q5NP42 Cluster: Putative uncharacterized protein; n=1; ... 33 6.2
UniRef50_UPI00006CFCC3 Cluster: hypothetical protein TTHERM_0059... 33 8.2
>UniRef50_P91641 Cluster: Mediator complex subunit 20; n=5;
Endopterygota|Rep: Mediator complex subunit 20 -
Drosophila melanogaster (Fruit fly)
Length = 252
Score = 121 bits (291), Expect = 2e-26
Identities = 54/96 (56%), Positives = 72/96 (75%)
Frame = +3
Query: 258 KNIHVIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFELGDFCVKLGSVTMNQNFKGILI 437
K + ++AD +FDLLM+K++ +TSKKQTKIES+G RFE GDF +KLGSVTM ++FKGILI
Sbjct: 83 KQVAIVADNIFDLLMLKMTNTFTSKKQTKIESRGARFEYGDFVIKLGSVTMMEHFKGILI 142
Query: 438 EVEYRPCVMANAVWGLLREFLQGLLGPTVPVQPPQH 545
E+EY+ CV+ W ++RE LQG LG V P +
Sbjct: 143 EIEYKSCVILAYCWEMIREMLQGFLGIAVNKDFPSY 178
Score = 97.5 bits (232), Expect = 3e-19
Identities = 43/78 (55%), Positives = 60/78 (76%), Gaps = 3/78 (3%)
Frame = +2
Query: 23 LGVTVLQQYPVPENKTGTYVVELLTKRILALGATQQGQFLVDCESYLSHPLM---GTTKT 193
+GVT+LQ YP+PE K+G ++++ L+KR+LALGAT GQFLVDCE+++S P +
Sbjct: 1 MGVTILQPYPLPEGKSGAHIIDQLSKRLLALGATHAGQFLVDCETFISTPQPHNGAPGRA 60
Query: 194 VHILHNSEQPASVFSILE 247
VH+LHNSE PAS FSI++
Sbjct: 61 VHVLHNSEYPASTFSIID 78
>UniRef50_Q9H944 Cluster: Mediator of RNA polymerase II
transcription subunit 20; n=27; Euteleostomi|Rep:
Mediator of RNA polymerase II transcription subunit 20 -
Homo sapiens (Human)
Length = 212
Score = 100 bits (240), Expect = 3e-20
Identities = 50/108 (46%), Positives = 70/108 (64%)
Frame = +3
Query: 270 VIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFELGDFCVKLGSVTMNQNFKGILIEVEY 449
+IAD FD+LM+KL + S K +KIE++G R++ DF VK+G+VTM + +GI +EVEY
Sbjct: 85 LIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEY 144
Query: 450 RPCVMANAVWGLLREFLQGLLGPTVPVQPPQHLISRMNEIYTPXDTTI 593
PCV+A+ W LL EFLQ LG P P +R + +Y P DT +
Sbjct: 145 GPCVVASDCWSLLLEFLQSFLGSHTP-GAPAVFGNRHDAVYGPADTMV 191
Score = 67.7 bits (158), Expect = 2e-10
Identities = 35/80 (43%), Positives = 51/80 (63%), Gaps = 5/80 (6%)
Frame = +2
Query: 23 LGVTVLQQYPVPENKTGTYVVELLTKRILALGATQQGQFLVDCESY-LSHPLMG----TT 187
+GVT + Q PV E K+ VELLT+++ LGA +QG F VDCE+Y + +G T
Sbjct: 1 MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTG 60
Query: 188 KTVHILHNSEQPASVFSILE 247
K ++++HNSE P S F++ E
Sbjct: 61 KLMYVMHNSEYPLSCFALFE 80
>UniRef50_A7RT91 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 187
Score = 90.2 bits (214), Expect = 4e-17
Identities = 41/91 (45%), Positives = 57/91 (62%), Gaps = 2/91 (2%)
Frame = +3
Query: 270 VIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFELGDFCVKLGSVTMNQNFKGILIEVEY 449
++A FD LM++L YT +K KIE KGP +E GDF +K+G +++ + KG+LIEVEY
Sbjct: 63 LVAQTGFDSLMVRLKAFYTQRKAAKIEVKGPSYEYGDFILKIGQISLGPSVKGVLIEVEY 122
Query: 450 RPCVMANAVWGLLREFLQGLLGP--TVPVQP 536
PC WGL+ EF+ LG T+P P
Sbjct: 123 LPCEDVEQCWGLISEFIASFLGTAFTLPSMP 153
Score = 50.0 bits (114), Expect = 5e-05
Identities = 25/56 (44%), Positives = 37/56 (66%), Gaps = 1/56 (1%)
Frame = +2
Query: 83 VELLTKRILALGATQQGQFLVDCESYLSHPL-MGTTKTVHILHNSEQPASVFSILE 247
VELL KR LGAT+ + VDCE+Y + L ++K +H++H++E P S F +LE
Sbjct: 3 VELLQKRAELLGATKTCNWCVDCETYQATSLHTSSSKVLHLVHSTEHPYSSFVVLE 58
>UniRef50_Q9N5A1 Cluster: Mediator protein 20; n=2;
Caenorhabditis|Rep: Mediator protein 20 - Caenorhabditis
elegans
Length = 198
Score = 55.6 bits (128), Expect = 1e-06
Identities = 31/111 (27%), Positives = 54/111 (48%), Gaps = 1/111 (0%)
Frame = +3
Query: 258 KNIHVIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFEL-GDFCVKLGSVTMNQNFKGIL 434
K+ + D FDL++ KLS + KIE G + L D+ +++G+ T KG++
Sbjct: 73 KSPKAVCDRGFDLILSKLSSGLIADNAGKIEIIGNEYSLYKDWMIRVGTATQGTTVKGVV 132
Query: 435 IEVEYRPCVMANAVWGLLREFLQGLLGPTVPVQPPQHLISRMNEIYTPXDT 587
+E+EY P ++ ++ EF++ + P I+ E YT DT
Sbjct: 133 VEIEYDPSIIVIQCKDMMIEFVKSVFNKYHETLPEIFKITEKPESYTALDT 183
>UniRef50_Q6NPF4 Cluster: At2g28230; n=9; Magnoliophyta|Rep:
At2g28230 - Arabidopsis thaliana (Mouse-ear cress)
Length = 219
Score = 48.8 bits (111), Expect = 1e-04
Identities = 31/85 (36%), Positives = 49/85 (57%), Gaps = 2/85 (2%)
Frame = +3
Query: 255 TKNIHVIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFELGDFCVKLGSV--TMNQNFKG 428
T+ I + AD L+M KL Q Y SK + G +++LGDF +++G V T ++N +G
Sbjct: 82 TQRIVLEADSSIQLIMEKL-QSYKSKVALYFD--GFQYQLGDFRLRVGKVVPTHSENVRG 138
Query: 429 ILIEVEYRPCVMANAVWGLLREFLQ 503
I++EVEY P ++ EFL+
Sbjct: 139 IVMEVEYLPISSMEKAQKVMEEFLE 163
>UniRef50_Q54FL3 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 195
Score = 48.8 bits (111), Expect = 1e-04
Identities = 27/73 (36%), Positives = 41/73 (56%), Gaps = 5/73 (6%)
Frame = +3
Query: 330 KKQTKIESKGPRFELGDFCVKLGSVTMNQNFKGILIEVEYRPC-VMANAVWG---LLREF 497
KK+ E+ ++ELGDF +++G + +GIL+EVEY C + + LL EF
Sbjct: 91 KKRQTTEALISKYELGDFIIRVGPIVFRTEIRGILVEVEYTACSTPVTSPFNCTKLLSEF 150
Query: 498 LQGLLGP-TVPVQ 533
+ G LGP P+Q
Sbjct: 151 INGNLGPFPEPIQ 163
>UniRef50_Q5DCZ1 Cluster: SJCHGC05429 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC05429 protein - Schistosoma
japonicum (Blood fluke)
Length = 278
Score = 46.8 bits (106), Expect = 5e-04
Identities = 34/106 (32%), Positives = 56/106 (52%), Gaps = 3/106 (2%)
Frame = +3
Query: 270 VIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFELGDFCVKLGSVTMNQ--NFKGILIEV 443
++AD F L Y KK+ ++E+KG +++LGDF V ++ M Q + KG LIEV
Sbjct: 81 LVADISFREFPGCLKAFYQRKKKLQVEAKGIKYQLGDFTVSFITLFMGQSASVKGYLIEV 140
Query: 444 EYRPCVMANAVWGLLREFLQGLLGPTVP-VQPPQHLISRMNEIYTP 578
C A+ + L R+ L+ + +P + PP + S I++P
Sbjct: 141 ----CFHASCMIHLSRDILKAFITQVLPDICPPAN--SSTEHIFSP 180
Score = 33.5 bits (73), Expect = 4.7
Identities = 21/70 (30%), Positives = 34/70 (48%)
Frame = +2
Query: 26 GVTVLQQYPVPENKTGTYVVELLTKRILALGATQQGQFLVDCESYLSHPLMGTTKTVHIL 205
GV+ + E V+E L K++ LGA + G+ ++ Y S+ KT +L
Sbjct: 3 GVSCVLLLNSSEGNCDPQVLENLMKKLELLGARKCGRVHIESAVYFSNFSRSQCKTFQVL 62
Query: 206 HNSEQPASVF 235
++E PAS F
Sbjct: 63 TSTEFPASCF 72
>UniRef50_Q55B78 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 116
Score = 33.5 bits (73), Expect = 4.7
Identities = 24/84 (28%), Positives = 35/84 (41%), Gaps = 2/84 (2%)
Frame = -1
Query: 592 IVVSXGVYISFILLIRC*GGWTGTVGXXXXXXXXXXXPQTALAITHGLYSTSIKIPLKF* 413
I +S + S + I +G+ G T + TH + STSI P
Sbjct: 16 ISISISISNSISISINSSSSCSGSSGSSRSTTHVTSSTSTGPSNTHTVSSTSIDSPTTHV 75
Query: 412 FIVTD--PSFTQKSPSSNLGPLLS 347
T PS T +PS+++GPL S
Sbjct: 76 TSSTSTGPSTTHATPSTSIGPLTS 99
>UniRef50_Q16PK6 Cluster: Serine protease, putative; n=7; Aedes
aegypti|Rep: Serine protease, putative - Aedes aegypti
(Yellowfever mosquito)
Length = 431
Score = 33.5 bits (73), Expect = 4.7
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +1
Query: 37 LTAISSAREQDRNICSGVTYKAYLSI 114
+T+ ++A++QD NICS Y AY+ +
Sbjct: 247 ITSFTAAKQQDENICSSTDYTAYIDV 272
>UniRef50_A2DDB2 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 191
Score = 33.5 bits (73), Expect = 4.7
Identities = 19/67 (28%), Positives = 33/67 (49%)
Frame = +3
Query: 255 TKNIHVIADGLFDLLMMKLSQHYTSKKQTKIESKGPRFELGDFCVKLGSVTMNQNFKGIL 434
TK+ ++ D F ++ + + KI+ G +LGDF V+ G + + GIL
Sbjct: 71 TKSTTLVGDANFQSVLKDIGLPFDKTLIFKID--GSMAQLGDFKVRYGVSIVKEKQVGIL 128
Query: 435 IEVEYRP 455
++EY P
Sbjct: 129 FDIEYLP 135
>UniRef50_Q1E4V1 Cluster: Putative uncharacterized protein; n=1;
Coccidioides immitis|Rep: Putative uncharacterized
protein - Coccidioides immitis
Length = 374
Score = 33.5 bits (73), Expect = 4.7
Identities = 14/43 (32%), Positives = 26/43 (60%), Gaps = 3/43 (6%)
Frame = +3
Query: 333 KQTKIESKGPRFELGDFCVKLGSVTMN---QNFKGILIEVEYR 452
+ T + + G FE+GDF +++G + Q +G ++E+EYR
Sbjct: 208 RHTMLVTGGTIFEVGDFRIRIGDLRQTQPVQRVRGCIVEIEYR 250
>UniRef50_Q5NP42 Cluster: Putative uncharacterized protein; n=1;
Zymomonas mobilis|Rep: Putative uncharacterized protein
- Zymomonas mobilis
Length = 304
Score = 33.1 bits (72), Expect = 6.2
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +3
Query: 453 PCVMANAVWGLLREFLQGLLGPTVPVQPPQHLISRMNEIYTPXD 584
P A AV G L ++L+G LG TVPV+P L E Y P +
Sbjct: 208 PLQQAKAVSGELSKYLRGKLGYTVPVRPVVSLPGWRIENYLPDE 251
>UniRef50_UPI00006CFCC3 Cluster: hypothetical protein
TTHERM_00599900; n=1; Tetrahymena thermophila SB210|Rep:
hypothetical protein TTHERM_00599900 - Tetrahymena
thermophila SB210
Length = 2606
Score = 32.7 bits (71), Expect = 8.2
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = -2
Query: 252 HFSRIEKTDAGCSELCNICTVFVVPMSGCDK*LSQSTRNCPC 127
+F++I+ T + CS++C CT+ + C + S NC C
Sbjct: 863 YFTKIDGTCSQCSQICKTCTLSATNCTQCFASNTLSPPNCAC 904
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 622,803,784
Number of Sequences: 1657284
Number of extensions: 11943635
Number of successful extensions: 27118
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 26390
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27113
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 50826451017
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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