Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_O12
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 144 1e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 142 4e-35
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 85 8e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 62 5e-11
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 27 3.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 6.8
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 25 6.8
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 9.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 144 bits (348), Expect = 1e-35
Identities = 67/122 (54%), Positives = 85/122 (69%)
Frame = +1
Query: 211 PQTRPSGVETILSTLSSARPELASTYPXAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGK 390
P+T + ST S + P +++VDLEP V+D+VRTG YR LFHPEQLITGK
Sbjct: 38 PETASQNSDGGFSTFFSETGQ-GKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGK 96
Query: 391 EDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMER 570
EDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ER
Sbjct: 97 EDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLER 156
Query: 571 LS 576
L+
Sbjct: 157 LA 158
Score = 104 bits (249), Expect = 1e-23
Identities = 45/64 (70%), Positives = 50/64 (78%)
Frame = +3
Query: 102 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 281
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 282 HVPR 293
+VPR
Sbjct: 61 YVPR 64
Score = 27.1 bits (57), Expect = 2.2
Identities = 9/15 (60%), Positives = 14/15 (93%)
Frame = +2
Query: 578 VDYGKKSKLEFAIYP 622
++Y KKSKL+F++YP
Sbjct: 159 MEYTKKSKLQFSVYP 173
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 142 bits (344), Expect = 4e-35
Identities = 59/96 (61%), Positives = 78/96 (81%)
Frame = +1
Query: 289 PXAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 468
P +++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 469 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 576
R++AD C+GLQGFL+FH LL+ERL+
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLN 162
Score = 97.1 bits (231), Expect = 2e-21
Identities = 49/69 (71%), Positives = 51/69 (73%), Gaps = 5/69 (7%)
Frame = +3
Query: 102 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 266
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 267 TGAGKHVPR 293
TG GK VPR
Sbjct: 60 TGQGKFVPR 68
Score = 28.3 bits (60), Expect = 0.97
Identities = 9/15 (60%), Positives = 14/15 (93%)
Frame = +2
Query: 578 VDYGKKSKLEFAIYP 622
++YGKKS L+F++YP
Sbjct: 163 MEYGKKSNLQFSVYP 177
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 85.0 bits (201), Expect = 8e-18
Identities = 39/95 (41%), Positives = 56/95 (58%)
Frame = +1
Query: 289 PXAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 468
P AV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 469 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERL 573
R+ A+ C LQGF + H LL+ ++
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKI 155
Score = 47.6 bits (108), Expect = 1e-06
Identities = 24/67 (35%), Positives = 32/67 (47%)
Frame = +3
Query: 102 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 281
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 282 HVPRCCL 302
+VPR L
Sbjct: 59 YVPRAVL 65
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 62.5 bits (145), Expect = 5e-11
Identities = 33/98 (33%), Positives = 53/98 (54%), Gaps = 2/98 (2%)
Frame = +1
Query: 289 PXAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLD 462
P A+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 463 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 576
I + AD L+GF + H L+ERL+
Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
Score = 55.6 bits (128), Expect = 6e-09
Identities = 27/66 (40%), Positives = 39/66 (59%)
Frame = +3
Query: 105 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 284
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 285 VPRCCL 302
+PR L
Sbjct: 61 IPRAIL 66
>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 649
Score = 26.6 bits (56), Expect = 3.0
Identities = 19/77 (24%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +1
Query: 235 ETILSTLSSARPELASTYPXAVFVDLE-PTVVDEVRTGTYRQLFHPEQLITG--KEDAAN 405
ET+L ++ + +A + A+ + L + + Y + E+ I +E AN
Sbjct: 545 ETLLLSVVRGKSGIAFLFFMAIMLKLLLGKAILAITQSRYESMQQREEKINSWERERKAN 604
Query: 406 NYARGHYTIGKEIVDLV 456
N+ RGH I K+ D +
Sbjct: 605 NFFRGHIEIDKKTKDFL 621
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 181 STASSLMARCPQTRPSGVETILSTLSSARPELAST 285
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 200 WPDAHRQDHRGWRRFFQHFLQR 265
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 6.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 91 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 213
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 629
Score = 25.4 bits (53), Expect = 6.8
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 552 FLIDGASLRLTTARSLNWSSPSTP 623
+L +GA L L T+ S++W TP
Sbjct: 225 YLPEGADLTLVTSYSVSWKYSDTP 248
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -3
Query: 155 ITDLDSGLANVYRDALTHFDLSF 87
+T +D GLA YRD TH + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,641,282
Number of Sequences: 5004
Number of extensions: 54602
Number of successful extensions: 159
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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