Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_J17
(570 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 75 6e-15
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 47 2e-06
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 29 0.36
SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 5.9
SPBP8B7.13 |||conserved fungal protein|Schizosaccharomyces pombe... 25 7.8
SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 25 7.8
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 25 7.8
SPAC26F1.12c |||conserved eukaryotic protein|Schizosaccharomyces... 25 7.8
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 75.4 bits (177), Expect = 6e-15
Identities = 43/135 (31%), Positives = 74/135 (54%), Gaps = 4/135 (2%)
Frame = +3
Query: 150 LSKSVELTFELPDSAEECFY-QTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQE 326
L+ ++ELTF+L + ++C+Y + Y V SGG +DV I+AP+++ + +
Sbjct: 17 LASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPSQKTVALGK 76
Query: 327 KMQYDSHQFTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGV-GDHATVLTQ-- 497
K + FT G+Y+ CF N STF+ K+V ME+ + E LP + D A +
Sbjct: 77 KRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDEAKDYKKDS 136
Query: 498 LETSAEEIHSALNRI 542
++++ EI +AL+ I
Sbjct: 137 MQSTVLEISTALSEI 151
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 47.2 bits (107), Expect = 2e-06
Identities = 23/67 (34%), Positives = 33/67 (49%)
Frame = +3
Query: 198 ECFYQTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQEKMQYDSHQFTALASGDY 377
ECFY+ + + S+ YQ GG VS+ I P QI++Q+ + FT G Y
Sbjct: 32 ECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKY 91
Query: 378 KVCFSNE 398
CF N+
Sbjct: 92 MYCFYND 98
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 29.5 bits (63), Expect = 0.36
Identities = 28/100 (28%), Positives = 43/100 (43%), Gaps = 7/100 (7%)
Frame = -3
Query: 490 RTVA*SPTPGRGCSGPTFSSIYTSLCENVLNSLLKHTL*SPDARAVNWCESYCIFSC*YI 311
R+ SPT PT + + E V N+L K+ S R + Y S Y+
Sbjct: 460 RSTTTSPTSAVASPAPTENEDSNAAIERVANTLRKNPTISRRTRRAGTMDRYATASSDYM 519
Query: 310 ICLLGA--SIFTLTSYCPPE--ITW---YSNEAAVSLSIV 212
LG+ ++ TL+ P+ TW + N + +S SIV
Sbjct: 520 ESNLGSLPNLSTLSLQSGPDSTATWHPEFPNSSGLSASIV 559
>SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 865
Score = 25.4 bits (53), Expect = 5.9
Identities = 11/45 (24%), Positives = 21/45 (46%)
Frame = +1
Query: 298 LTDKLYISKRRCNMIHTSSPLWRLVITKCVLVMSLVHFHINLCIW 432
LT K + + + WR V+ + +L + L + + LC+W
Sbjct: 613 LTQKYILMYVSNSSTESGGGYWRPVVNRILLGLELANIILFLCLW 657
>SPBP8B7.13 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 25.0 bits (52), Expect = 7.8
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 334 NMIHTSSPLWRLVITKCVLVMSLVHFH 414
N++H LW C +V+SLV+FH
Sbjct: 220 NLLHKR--LWISFFFACFVVLSLVYFH 244
>SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 629
Score = 25.0 bits (52), Expect = 7.8
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 450 EQPLPGVGDHATVLTQLETSAEEIH 524
+ P PGVG A + LE S E++H
Sbjct: 397 DHPSPGVGRGAPEIDILEGSTEKLH 421
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 25.0 bits (52), Expect = 7.8
Identities = 17/105 (16%), Positives = 44/105 (41%)
Frame = +3
Query: 240 LEYQVISGGQYDVSVKIEAPNRQIIYQQEKMQYDSHQFTALASGDYKVCFSNEFSTFSHK 419
++ Q+ S Q + + +++ N +I+ ++ + + + + ++ N F H+
Sbjct: 819 MKAQIESSNQ-EYTATVDSMNSRILELSNDLRVANSKLSECSDDVRRLTLQNSFDLREHQ 877
Query: 420 LVYMELNVGPEQPLPGVGDHATVLTQLETSAEEIHSALNRIIDHQ 554
+ ++L + + TV QLE E+ L + + Q
Sbjct: 878 TLVLQLQSNITELKQDITLQRTVRNQLEIQTTELKERLKFMEERQ 922
>SPAC26F1.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 356
Score = 25.0 bits (52), Expect = 7.8
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -1
Query: 483 LHDLLHQEEAAPVQHLVPYT 424
LHD Q VQHL+PYT
Sbjct: 11 LHDQNPQVRMLAVQHLLPYT 30
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,357,984
Number of Sequences: 5004
Number of extensions: 48659
Number of successful extensions: 115
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 242064240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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