Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_J07
(639 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1442.16c |zta1|SPCC285.01c|NADPH quinone oxidoreductase/ARE-... 49 7e-07
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 38 0.001
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 37 0.002
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 36 0.007
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 35 0.011
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 30 0.25
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 27 3.0
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 25 7.0
SPBC19G7.14c |cog5||Golgi transport complex subunit Cog5 |Schizo... 25 9.2
>SPCC1442.16c |zta1|SPCC285.01c|NADPH quinone
oxidoreductase/ARE-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 329
Score = 48.8 bits (111), Expect = 7e-07
Identities = 31/89 (34%), Positives = 48/89 (53%), Gaps = 1/89 (1%)
Frame = +2
Query: 371 FVRXAIDSPPKTPFILGFECAGEIEQVGENV-TNFKVGDQVVALPEYRAWAELVSVPAQY 547
++R + + P P+I G E AG + VG+ V +FKVGD+VV L + A+A+ +VP
Sbjct: 47 YLRTGLYTAP-LPYIPGKEAAGVVAAVGDKVEADFKVGDRVVYLTPFGAYAQYTNVPTTL 105
Query: 548 VYALPEGMSXLDAVAITTNYVVAYLLLFE 634
V + E + A A + AY L+ E
Sbjct: 106 VSKVSEKIPLKIASAALLQGLTAYTLIEE 134
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 37.9 bits (84), Expect = 0.001
Identities = 18/31 (58%), Positives = 21/31 (67%)
Frame = +2
Query: 407 PFILGFECAGEIEQVGENVTNFKVGDQVVAL 499
P ILG E AG +E VG VT +VGD V+AL
Sbjct: 67 PVILGHEGAGIVESVGPQVTTVQVGDPVIAL 97
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = +2
Query: 386 IDSPPKTPFILGFECAGEIEQVGENVTNFKVGDQVVAL 499
+D P +LG E AG +E +GE V N + GD V+ L
Sbjct: 57 VDPEGAFPIVLGHEGAGIVESIGEGVINVRPGDHVILL 94
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 35.5 bits (78), Expect = 0.007
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +2
Query: 401 KTPFILGFECAGEIEQVGENVTNFKVGDQVVALP 502
K P ILG E AG + +VG+ V++ K GD V P
Sbjct: 60 KKPMILGHESAGVVVEVGKGVSSLKPGDPVAVEP 93
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 34.7 bits (76), Expect = 0.011
Identities = 18/48 (37%), Positives = 32/48 (66%)
Frame = +1
Query: 250 LTGFGGLKTVKILKKPEPTVGEGEVLIRVKACGLNFQDLIVRQGRHRL 393
++GF LK + + P+ + GEVL+++KA LN++DLI+ +G + L
Sbjct: 11 ISGFDQLKPEEY-EVPQK-LNPGEVLVKLKAASLNYRDLIITKGLYPL 56
Score = 33.9 bits (74), Expect = 0.020
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +2
Query: 395 PPKTPFILGFECAGEIEQVGENVTNFKVGDQVV 493
P + P + G + AG IE+VGE+V F+ GD VV
Sbjct: 57 PLQLPVVPGSDGAGIIEKVGEDVEGFEKGDSVV 89
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 30.3 bits (65), Expect = 0.25
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 413 ILGFECAGEIEQVGENVTNFKVGDQVV 493
ILG E G + + G+ V N ++GD+VV
Sbjct: 94 ILGHESCGIVAEKGDEVNNLEIGDRVV 120
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 26.6 bits (56), Expect = 3.0
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +3
Query: 438 KSSKLAKMSPILRWAIKWWL 497
+SS L K+ P+LR+ I++WL
Sbjct: 154 RSSHLLKVRPLLRFLIEFWL 173
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 25.4 bits (53), Expect = 7.0
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
Frame = -2
Query: 437 HQRIRSPR*KESSVESRWRP*R--TIKSWKFRPQAFTRIRTSPS 312
H ++P S RWR R + K++ FRP TR+ ++PS
Sbjct: 73 HPPSKNPTSSSLSTRKRWRQKRLWSFKNFPFRPP--TRLTSTPS 114
>SPBC19G7.14c |cog5||Golgi transport complex subunit Cog5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 411
Score = 25.0 bits (52), Expect = 9.2
Identities = 15/62 (24%), Positives = 27/62 (43%), Gaps = 2/62 (3%)
Frame = +3
Query: 402 RLLSSWASNALVKSSKLAKMSPILRWAIKWWLFPSTALGPSWYLYRRS--MCTRCPKECL 575
RLLSS A+++ + ++SP R + PS A W + ++ +C+
Sbjct: 241 RLLSSRIQKAIIQFDSIFQLSPTTRRLLHNSTDPSAANSTIWSKFEDGWYQISKIGAQCV 300
Query: 576 XW 581
W
Sbjct: 301 FW 302
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,271,626
Number of Sequences: 5004
Number of extensions: 42165
Number of successful extensions: 118
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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