Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_J06
(483 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein L23|Schizosac... 165 4e-42
SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein L23|Schizosacc... 165 4e-42
SPBC887.07 |mrpl38||mitochondrial ribosomal protein subunit L38|... 47 2e-06
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 28 0.85
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 26 2.6
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 26 3.4
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 3.4
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 3.4
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 25 4.5
SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharom... 25 4.5
SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces po... 25 6.0
SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|c... 25 7.9
SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 25 7.9
>SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 165 bits (400), Expect = 4e-42
Identities = 78/123 (63%), Positives = 97/123 (78%)
Frame = +1
Query: 67 AGAKFRISLGLPXGSSNQLRRQHRXQRICM*SLSKXIKGRLNRLPAAGSGDMIVATVKKG 246
+G K+R++LGLP + + + + S+ RLNRLPAA GDM++ATVKKG
Sbjct: 9 SGTKYRMTLGLPVQAIMNCADNSGAKNLYIVSVF-GTGARLNRLPAASCGDMVLATVKKG 67
Query: 247 KPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVIVNNKGEMKGSAITGPVAKECADL 426
KP+LRKK+MPA+V+RQRK +RR+DGV++YFEDNAGVIVN KGEMKGSAITGPVAKECADL
Sbjct: 68 KPDLRKKIMPAIVVRQRKAWRRKDGVYLYFEDNAGVIVNPKGEMKGSAITGPVAKECADL 127
Query: 427 WPR 435
WPR
Sbjct: 128 WPR 130
>SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 139
Score = 165 bits (400), Expect = 4e-42
Identities = 78/123 (63%), Positives = 97/123 (78%)
Frame = +1
Query: 67 AGAKFRISLGLPXGSSNQLRRQHRXQRICM*SLSKXIKGRLNRLPAAGSGDMIVATVKKG 246
+G K+R++LGLP + + + + S+ RLNRLPAA GDM++ATVKKG
Sbjct: 9 SGTKYRMTLGLPVQAIMNCADNSGAKNLYIVSVF-GTGARLNRLPAASCGDMVLATVKKG 67
Query: 247 KPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVIVNNKGEMKGSAITGPVAKECADL 426
KP+LRKK+MPA+V+RQRK +RR+DGV++YFEDNAGVIVN KGEMKGSAITGPVAKECADL
Sbjct: 68 KPDLRKKIMPAIVVRQRKAWRRKDGVYLYFEDNAGVIVNPKGEMKGSAITGPVAKECADL 127
Query: 427 WPR 435
WPR
Sbjct: 128 WPR 130
>SPBC887.07 |mrpl38||mitochondrial ribosomal protein subunit
L38|Schizosaccharomyces pombe|chr 2|||Manual
Length = 126
Score = 46.8 bits (106), Expect = 2e-06
Identities = 28/79 (35%), Positives = 41/79 (51%), Gaps = 9/79 (11%)
Frame = +1
Query: 205 AGSGDMIVATVKKGKPEL---------RKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVI 357
A GD +V VKK + R + A+++R + P RR DG ++ F+DNA V+
Sbjct: 31 ASLGDEVVVVVKKARSGSSVTAANKVKRGDIHHAIIVRTKSPVRRPDGRYVRFDDNACVL 90
Query: 358 VNNKGEMKGSAITGPVAKE 414
VN + E G+ I VA E
Sbjct: 91 VNKECEPLGTRILSVVANE 109
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 27.9 bits (59), Expect = 0.85
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -2
Query: 98 RPREIRNFAPAGTTTSSLRHFVVSK 24
RP+E NF AG T+ L++++VSK
Sbjct: 1346 RPKEEINFQEAGVLTAPLKNWLVSK 1370
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 26.2 bits (55), Expect = 2.6
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = +1
Query: 238 KKGKPELRKKVMPAVVIRQRK---PFRRRDGVFIYFEDNA 348
KKG P RK P+++ PF RD +FI E NA
Sbjct: 337 KKGAPVFRKH-FPSILQELNSLDPPFYERDAIFIVDEGNA 375
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.8 bits (54), Expect = 3.4
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = -2
Query: 386 EPFISPLLLTMTPALSSKYINTPS 315
+P+ P+L + P L Y++ PS
Sbjct: 275 QPYSGPILASTAPTLDGSYLSNPS 298
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 25.8 bits (54), Expect = 3.4
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = -2
Query: 428 HRSAHSLATGPVMAEPFISPLLLTMTPALSSKYINTPSRLLNGFRCLMTTA 276
+ + H GP++ ++ L P +S Y+N + L+GF+ ++ A
Sbjct: 963 YNATHFEHIGPMLEATWMPILAALSNPLQNSDYVNELNMCLDGFQLVVRIA 1013
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.8 bits (54), Expect = 3.4
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = -2
Query: 434 RGHRSAHSLATGPVMAEPFISPLLL-TMTPALSSKYINTPSRLLNGF 297
R H+ AH+ AEP ++P++ + P+++ +TPS L F
Sbjct: 60 RRHKDAHAYDMSLTDAEPALAPVMSPAIPPSMNYPTPSTPSVLPESF 106
>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
Apc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1458
Score = 25.4 bits (53), Expect = 4.5
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = +1
Query: 223 IVATVKKGKPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGV 354
IV KGK L + P++ I R G +Y ED GV
Sbjct: 309 IVVLSSKGKVSLESPMSPSLPIEGTFRSFRVHGATLYLEDTDGV 352
>SPBC887.09c |||leucine-rich repeat protein Sog2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 886
Score = 25.4 bits (53), Expect = 4.5
Identities = 17/65 (26%), Positives = 27/65 (41%)
Frame = -2
Query: 356 MTPALSSKYINTPSRLLNGFRCLMTTAGITFFRSSGLPFLTVATIMSPEPAAGSLFRRPL 177
+ ALSS+Y S L +T +T SS T +++ +P P + R P
Sbjct: 777 LAKALSSEYTFQKSVLAGLSAATRSTKDLTILLSSSARHYTESSLATPVPLMSPIARVPA 836
Query: 176 IXLDS 162
L +
Sbjct: 837 TPLSA 841
>SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 743
Score = 25.0 bits (52), Expect = 6.0
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = -1
Query: 345 IVLKVYKYSITPSERFPLPDDHCRHYLFPEFR 250
+V +++++ E FPLP LFP R
Sbjct: 438 LVSEIFRHPTHTDEDFPLPSPSLSSLLFPTLR 469
>SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 519
Score = 24.6 bits (51), Expect = 7.9
Identities = 16/56 (28%), Positives = 22/56 (39%)
Frame = -1
Query: 357 YDTRIVLKVYKYSITPSERFPLPDDHCRHYLFPEFRFTLFDCGHNHVPGTGRRQSV 190
Y+TR + K + +E PLP LF E F C + G QS+
Sbjct: 11 YNTRNMEKESSKDLVQNEDTPLPVQKISMSLFHEIVFVFIACTAQLMTQAGLGQSI 66
>SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein
Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 968
Score = 24.6 bits (51), Expect = 7.9
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = -1
Query: 132 LSAQLITAPXWETQGDTEFRSRXNHHVLS*TFCCIKN 22
+S+ + P + G+ +R H +L FCC+KN
Sbjct: 527 VSSYRLVKPILNSYGN-HVLNRIQHDILENHFCCLKN 562
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,970,855
Number of Sequences: 5004
Number of extensions: 38948
Number of successful extensions: 100
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 94
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 186042952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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