Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_H01
(668 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 243 2e-65
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 243 2e-65
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 3.2
SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 25 7.5
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 7.5
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 25 7.5
SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 25 7.5
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 243 bits (595), Expect = 2e-65
Identities = 118/170 (69%), Positives = 136/170 (80%), Gaps = 1/170 (0%)
Frame = +1
Query: 124 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 300
K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64
Query: 301 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTXKASAHLEGGAKKVIISXP 480
G LV++GN I V +ERDP I W +GA+YV+ESTGVFTT ASAHL+GGAK+VIIS P
Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124
Query: 481 XADAPMXVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNLEXGXGL 630
DAPM VVGVN ++PS KVISNASCTTNCLAPLAKVI+D GL
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGL 174
Score = 35.1 bits (77), Expect = 0.009
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = +2
Query: 578 LPHLQRLFMITLKXVEGLMTTVHATTATQK 667
L L ++ T EGLMTTVHATTATQK
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTATQK 186
>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 335
Score = 243 bits (594), Expect = 2e-65
Identities = 118/170 (69%), Positives = 136/170 (80%), Gaps = 1/170 (0%)
Frame = +1
Query: 124 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 300
K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE +
Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64
Query: 301 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTXKASAHLEGGAKKVIISXP 480
G LV++G+ I V +ERDP I W +GAEYV+ESTGVFTT ASAHL+GGAK+VIIS P
Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124
Query: 481 XADAPMXVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNLEXGXGL 630
DAPM VVGVNL ++PS KVISNASCTTNCLAPLAKVI+D GL
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGL 174
Score = 35.1 bits (77), Expect = 0.009
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = +2
Query: 578 LPHLQRLFMITLKXVEGLMTTVHATTATQK 667
L L ++ T EGLMTTVHATTATQK
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTATQK 186
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 26.6 bits (56), Expect = 3.2
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = -3
Query: 330 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 175
+F + N+ESIL+L L + + E I++IV+ +D R HLS L +
Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392
>SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1888
Score = 25.4 bits (53), Expect = 7.5
Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = -3
Query: 333 GNFVTVNNKESILNLNTALKTAMGGIILEKINHIVKTDERVIYSDHL-SSLFNRSTEHQT 157
GN +VN+K+ +N+N KTA ++H+ E ++ L SSLF+ + ++
Sbjct: 519 GNIFSVNSKKHSVNINA--KTAAN----NGLSHLQNFSEELLKKRKLFSSLFSNNVSYKK 572
Query: 156 ANAAK 142
+ K
Sbjct: 573 SKKLK 577
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.4 bits (53), Expect = 7.5
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +1
Query: 139 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 249
GF RI +R EKG QVV D I + + +L+
Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 48 LHLVLSVRGGGQLFNK 95
LHLVL +RGG Q+F K
Sbjct: 67 LHLVLRLRGGMQIFVK 82
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 48 LHLVLSVRGGGQLFNK 95
LHLVL +RGG Q+F K
Sbjct: 143 LHLVLRLRGGMQIFVK 158
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 48 LHLVLSVRGGGQLFNK 95
LHLVL +RGG Q+F K
Sbjct: 219 LHLVLRLRGGMQIFVK 234
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 48 LHLVLSVRGGGQLFNK 95
LHLVL +RGG Q+F K
Sbjct: 295 LHLVLRLRGGMQIFVK 310
>SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 609
Score = 25.4 bits (53), Expect = 7.5
Identities = 16/77 (20%), Positives = 38/77 (49%), Gaps = 6/77 (7%)
Frame = +1
Query: 106 LADNMSKIGINGFGRIGRLVLRASIEKGAQVVAINDPFIGLD------YMVYLFKYDSTH 267
+ D+ ++ +N F +G+L++ + ++ + ND ++ YM Y+F+ +
Sbjct: 515 VGDHTGQLWLNVFDDVGKLIMHKTADE-LNDLQENDENAFMNCMAEACYMPYIFQCRAKQ 573
Query: 268 GRFKGSVEVQDGFLVVN 318
FKG + V+ + +N
Sbjct: 574 DNFKGEMRVRYTVMSIN 590
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,703,813
Number of Sequences: 5004
Number of extensions: 53097
Number of successful extensions: 172
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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