Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P05_F_C19
(401 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 211 1e-55
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 211 1e-55
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 211 1e-55
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 211 1e-55
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 123 4e-29
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 108 1e-24
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 74 4e-14
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 69 9e-13
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 51 3e-07
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 50 4e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 50 4e-07
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 45 2e-05
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 44 4e-05
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 39 0.001
At5g13650.2 68418.m01585 elongation factor family protein contai... 39 0.001
At5g13650.1 68418.m01584 elongation factor family protein contai... 39 0.001
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 36 0.013
At5g37055.1 68418.m04446 zinc finger (HIT type) family protein c... 27 6.2
At3g14080.2 68416.m01780 small nuclear ribonucleoprotein, putati... 27 6.2
At3g14080.1 68416.m01779 small nuclear ribonucleoprotein, putati... 27 6.2
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 211 bits (515), Expect = 1e-55
Identities = 98/108 (90%), Positives = 101/108 (93%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 244
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 245 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQ
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQ 108
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 211 bits (515), Expect = 1e-55
Identities = 98/108 (90%), Positives = 101/108 (93%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 244
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 245 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQ
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQ 108
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 211 bits (515), Expect = 1e-55
Identities = 98/108 (90%), Positives = 101/108 (93%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 244
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 245 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQ
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQ 108
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 211 bits (515), Expect = 1e-55
Identities = 98/108 (90%), Positives = 101/108 (93%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 244
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 245 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQ
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQ 108
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 123 bits (297), Expect = 4e-29
Identities = 50/103 (48%), Positives = 77/103 (74%)
Frame = +2
Query: 80 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 259
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 260 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +Q
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQ 340
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 108 bits (260), Expect = 1e-24
Identities = 47/109 (43%), Positives = 74/109 (67%)
Frame = +2
Query: 74 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 253
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 254 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQG*XG 400
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQ G
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIG 206
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 73.7 bits (173), Expect = 4e-14
Identities = 40/109 (36%), Positives = 57/109 (52%)
Frame = +2
Query: 62 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 241
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 242 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQ 165
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 69.3 bits (162), Expect = 9e-13
Identities = 40/106 (37%), Positives = 56/106 (52%)
Frame = +2
Query: 71 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 250
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 251 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 388
E++RGITI A ++ET+K + +D PGH D++KNMITG +Q
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQ 153
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.2 bits (117), Expect = 3e-07
Identities = 33/94 (35%), Positives = 49/94 (52%)
Frame = +2
Query: 77 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 256
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 257 AERERGITIDIALWKFETSKYYVTIIDAPGHRDF 358
E+ERGITI A K+ + IID PGH DF
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDF 174
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 50.4 bits (115), Expect = 4e-07
Identities = 34/90 (37%), Positives = 46/90 (51%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 268
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 269 RGITIDIALWKFETSKYYVTIIDAPGHRDF 358
+GITI A Y V IID PGH DF
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDF 146
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 50.4 bits (115), Expect = 4e-07
Identities = 34/90 (37%), Positives = 46/90 (51%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 268
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 269 RGITIDIALWKFETSKYYVTIIDAPGHRDF 358
+GITI A Y V IID PGH DF
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDF 146
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 45.2 bits (102), Expect = 2e-05
Identities = 31/96 (32%), Positives = 44/96 (45%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 268
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 269 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMIT 376
R IT+ + + Y + +ID+PGH DF + T
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVST 93
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 44.0 bits (99), Expect = 4e-05
Identities = 31/94 (32%), Positives = 44/94 (46%), Gaps = 4/94 (4%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 268
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 269 RGITIDIAL----WKFETSKYYVTIIDAPGHRDF 358
RGITI + + +E + + + +ID PGH DF
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDF 165
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 38.7 bits (86), Expect = 0.001
Identities = 39/111 (35%), Positives = 49/111 (44%), Gaps = 9/111 (8%)
Frame = +2
Query: 53 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 232
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 233 AWVLDKLKAERERGITI---------DIALWKFETSKYYVTIIDAPGHRDF 358
LDKL +RERGIT+ + + E S Y + +ID PGH DF
Sbjct: 103 ---LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDF 148
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 38.7 bits (86), Expect = 0.001
Identities = 29/90 (32%), Positives = 40/90 (44%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 268
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 269 RGITIDIALWKFETSKYYVTIIDAPGHRDF 358
RGITI V IID PGH DF
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDF 159
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 38.7 bits (86), Expect = 0.001
Identities = 29/90 (32%), Positives = 40/90 (44%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 268
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 269 RGITIDIALWKFETSKYYVTIIDAPGHRDF 358
RGITI V IID PGH DF
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDF 158
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 35.5 bits (78), Expect = 0.013
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 4/94 (4%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 256
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 257 AERERGITIDIALWKFETSKYYVTIIDAPGHRDF 358
E ++ + ++Y + +ID+PGH DF
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDF 111
>At5g37055.1 68418.m04446 zinc finger (HIT type) family protein
contains Pfam profile: PF04438 HIT zinc finger
Length = 171
Score = 26.6 bits (56), Expect = 6.2
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 242 IPKHI*RILYPFPGPPSRTSRWYVC 168
+P H+ L GPPS +SR Y C
Sbjct: 110 LPSHVPTYLKAAVGPPSSSSRRYFC 134
>At3g14080.2 68416.m01780 small nuclear ribonucleoprotein, putative
/ snRNP, putative / Sm protein, putative similar to U6
snRNA-associated Sm-like protein LSm1 (Small nuclear
ribonuclear CaSm, Cancer-associated Sm-like) [Homo
sapiens] SWISS-PROT:O15116; contains Pfam profile:
PF01423 Sm protein
Length = 128
Score = 26.6 bits (56), Expect = 6.2
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM 211
N+V+IG +D+ + H+I + + + +K E+EA E+
Sbjct: 75 NVVLIGELDTEREELPPHMI-RVSEAEIKRAQKVEREASEL 114
>At3g14080.1 68416.m01779 small nuclear ribonucleoprotein, putative
/ snRNP, putative / Sm protein, putative similar to U6
snRNA-associated Sm-like protein LSm1 (Small nuclear
ribonuclear CaSm, Cancer-associated Sm-like) [Homo
sapiens] SWISS-PROT:O15116; contains Pfam profile:
PF01423 Sm protein
Length = 128
Score = 26.6 bits (56), Expect = 6.2
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM 211
N+V+IG +D+ + H+I + + + +K E+EA E+
Sbjct: 75 NVVLIGELDTEREELPPHMI-RVSEAEIKRAQKVEREASEL 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,287,699
Number of Sequences: 28952
Number of extensions: 152957
Number of successful extensions: 462
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 437
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 454
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 585758608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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